BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0265.4
(822 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232867 similar to UP|Q9MA68 (Q9MA68) F2J6.13 protein, partial ... 139 7e-33
TC231258 similar to UP|Q886T6 (Q886T6) Sensory box histidine kin... 125 6e-29
TC232398 94 1e-28
BQ133845 108 1e-23
TC222094 77 6e-23
BU546409 50 3e-06
CF920352 49 7e-06
BF595237 48 2e-05
TC232616 similar to GB|AAD25756.1|4587525|T5I8 Contains the PF|0... 31 1.9
TC232345 31 1.9
TC218742 similar to GB|AAL57703.1|18086557|AY072008 AT5g04060/F8... 30 4.3
TC217912 weakly similar to UP|MFP1_ARATH (Q9LW85) MAR binding fi... 30 5.6
TC219105 similar to GB|AAM10098.1|20148415|AY081536 endomembrane... 29 9.6
TC216883 similar to GB|AAF86349.1|9255891|AF279129 FIN219 {Arabi... 29 9.6
TC230567 similar to UP|Q9FGU0 (Q9FGU0) Arabidopsis thaliana geno... 29 9.6
>TC232867 similar to UP|Q9MA68 (Q9MA68) F2J6.13 protein, partial (3%)
Length = 1144
Score = 139 bits (349), Expect = 7e-33
Identities = 82/256 (32%), Positives = 136/256 (53%), Gaps = 10/256 (3%)
Frame = -3
Query: 180 PAFINLVSQSQYGGGSSEDPHAHMERFIRNCNTYRVSNVSSEAIRLSLFPFSLKDAAEEW 239
P+ I L+ + + G +EDP+AH+ +I CNT R++ V ++AIRLSL FSL A+ W
Sbjct: 815 PSLIQLIQSNFFHGLPNEDPYAHLATYIEICNTIRLAGVPADAIRLSLLSFSLSGEAKRW 636
Query: 240 LNSQPQGSITTWEDLAEKFTTRFFPRALLRKLKKDIMNFVQSAEENLYEAWERFKKLLRK 299
L+S S+ +W+++ EKF ++FP + + K I +F Q +E+L EA ERF+ LLRK
Sbjct: 635 LHSFKGNSLKSWDEVVEKFLKKYFPESKTAEGKAAISSFHQFPDESLSEALERFRGLLRK 456
Query: 300 CPQHNLTQAELVATFYDGLEYSWRFGLDTASSGEFDALPPQAGYDLIEKMAARAMNSEND 359
P H ++ + F D L + +D + G+ P DLIE MAA + D
Sbjct: 455 TPTHGFSEPIQLNIFIDELRPESKQLVDASVGGKIKMKTPDEAMDLIESMAASDIAILRD 276
Query: 360 R---QTRRRMFEFEAYDKLLASNKQISEK-------VAEIQNHIKVTKLVGASVAKVECV 409
R T++ + E + D LLA NK +S++ ++++ + + +S+ +V
Sbjct: 275 RAHIPTKKSLLELTSQDTLLAQNKLLSKQLETLTKTLSKLPTQLHSAQTSHSSILQVTGC 96
Query: 410 TRGGPNFSNSCTMTNQ 425
T G + C + N+
Sbjct: 95 TIFGEAHESGCCIPNE 48
>TC231258 similar to UP|Q886T6 (Q886T6) Sensory box histidine kinase/response
regulator, partial (3%)
Length = 1374
Score = 125 bits (315), Expect = 6e-29
Identities = 76/240 (31%), Positives = 122/240 (50%), Gaps = 2/240 (0%)
Frame = +2
Query: 149 RSLRELTSAPMSYDYPGSIVFPEGVGNFKLRPAFINLVSQSQYGGGSSEDPHAHMERFIR 208
R+LRE+ + +Y+ + +P+ + L+ I+L+ + + G EDPH H++ F
Sbjct: 437 RTLREMVAPDFTYESLCN-QYPDEDVPYVLKTGLIHLLPK--FHGLVGEDPHKHLKEFHI 607
Query: 209 NCNTYRVSNVSSEAIRLSLFPFSLKDAAEEWLNSQPQGSITTWEDLAEKFTTRFFPRALL 268
C+T + +V + I L FP SL+ A++WL SI W+DL F +FFP +
Sbjct: 608 VCSTMKPPDVQEDHIFLKAFPHSLEGVAKDWLYYLAPRSIFNWDDLKRVFLEKFFPASRT 787
Query: 269 RKLKKDIMNFVQSAEENLYEAWERFKKLLRKCPQHNLTQAELVATFYDGLEYSWRFGLDT 328
++KDI Q + E+LYE WERFKK CP H +++ L+ FY+ L R +D
Sbjct: 788 TTIRKDISGIRQLSRESLYEYWERFKKSCASCPHHQISKQLLL*YFYEELSNMKRSMIDA 967
Query: 329 ASSGEFDALPPQAGYDLIEKMAARA--MNSENDRQTRRRMFEFEAYDKLLASNKQISEKV 386
AS G + P +LI+KMA+ + ++ ND R + E NK + E +
Sbjct: 968 ASGGALGDMTPTEARNLIKKMASNSQQFSARNDAIVLRGVHEVATDSSSSTENKSLRENL 1147
>TC232398
Length = 1054
Score = 93.6 bits (231), Expect(2) = 1e-28
Identities = 41/93 (44%), Positives = 67/93 (71%)
Frame = +1
Query: 683 RRLNLGEVTPTMLSLQMADRSLKTPYGIIEDVMVKVDKYVFPIDFVVLDMEEDAKIPLIL 742
RR+ ++ PT ++LQ+AD S+ +G++ED++VKV + +F +DFV++D+EEDA+I LIL
Sbjct: 172 RRIGNQKIEPTRMTLQLADHSITRSFGVVEDILVKVHQLIFLVDFVIMDIEEDAEIRLIL 351
Query: 743 GRPFLATARAKIDVDKGQLILRVGKDKVRFSVF 775
G PF+ TA+ +D+ KG L + V K F++F
Sbjct: 352 GWPFMVTAKCVVDMGKGNLEMSVEDQKATFNLF 450
Score = 52.0 bits (123), Expect(2) = 1e-28
Identities = 28/60 (46%), Positives = 40/60 (66%)
Frame = +2
Query: 591 LHINIPFSEALEQMLIYAKFMRDILSKKRKLSEVDETVMLTEECSAILQRKIPRKRRDPG 650
L I IPF E ++QM +Y KF++DIL KK K ET+++ E C A++Q K+P K +D G
Sbjct: 2 LEITIPFGERIQQMPLYKKFLKDILIKKGKYIN-SETIVVGEYCRALIQ-KLPPKFKDLG 175
>BQ133845
Length = 389
Score = 108 bits (269), Expect = 1e-23
Identities = 55/123 (44%), Positives = 83/123 (66%)
Frame = +2
Query: 562 ELSKIPFPKALANKNLDKQFSKFLEVFKKLHINIPFSEALEQMLIYAKFMRDILSKKRKL 621
E ++P+P + K+ ++ +KFL++FKKL I +PF EAL+QM +YA F++D+L+KK
Sbjct: 23 ECKEVPYPLVPS*KDKEQHLAKFLDIFKKLEITLPFEEALQQMPLYANFLKDMLTKKNWY 202
Query: 622 SEVDETVMLTEECSAILQRKIPRKRRDPGSFTIPVEIEGLSKVDALCDLGASINLMPLSM 681
D+ +++ CSA++QR +P DPG T+P I ++ AL DLGASINLMPLSM
Sbjct: 203 IHSDK-IVVEGNCSAVIQRILPP*HTDPGFVTMPCSIGEVAVGKALIDLGASINLMPLSM 379
Query: 682 FRR 684
R+
Sbjct: 380 CRQ 388
>TC222094
Length = 984
Score = 77.0 bits (188), Expect(2) = 6e-23
Identities = 41/112 (36%), Positives = 60/112 (52%), Gaps = 1/112 (0%)
Frame = -2
Query: 156 SAPMSYDYPGSIVFPE-GVGNFKLRPAFINLVSQSQYGGGSSEDPHAHMERFIRNCNTYR 214
S+P+ Y SI PE NF + I L+ + + G +EDP+AH+ +I CNT +
Sbjct: 830 SSPIIPQYFTSIARPEVQTANFSYPYSLIQLIQGNLFYGLPTEDPYAHLATYIDICNTVK 651
Query: 215 VSNVSSEAIRLSLFPFSLKDAAEEWLNSQPQGSITTWEDLAEKFTTRFFPRA 266
+ V +AI L LF FSL A WL S ++ TW + EKF ++FP +
Sbjct: 650 IVGVPEDAIHLDLFCFSLAGEARTWLRSFKGNNLRTWNEXXEKFLKKYFPES 495
Score = 49.7 bits (117), Expect(2) = 6e-23
Identities = 28/80 (35%), Positives = 43/80 (53%)
Frame = -3
Query: 272 KKDIMNFVQSAEENLYEAWERFKKLLRKCPQHNLTQAELVATFYDGLEYSWRFGLDTASS 331
K +I +F Q E+L EA +RF LL K P H ++ + F DG++ + LD ++
Sbjct: 478 KVEISSFHQHPHESLSEALDRFHGLLWKTPTHGFSEPVQLNIFIDGMQPHSKQLLDASAG 299
Query: 332 GEFDALPPQAGYDLIEKMAA 351
G+ P+ +LIE MAA
Sbjct: 298 GKIKLKTPEEAIELIENMAA 239
Score = 35.4 bits (80), Expect = 0.10
Identities = 23/56 (41%), Positives = 31/56 (55%)
Frame = -3
Query: 452 LEELLESFINRMESNYKIQDAAIKNLKSQFGQLAKQMAKIPQGKFSPNALILSKHE 507
LEELL F+ S+ K DAAI+NL+ QLAK + + P F+ N + K E
Sbjct: 181 LEELLVQFMQETRSHQKSTDAAIRNLEV---QLAKLVPERPTETFAVNTKMKPKKE 23
>BU546409
Length = 528
Score = 50.4 bits (119), Expect = 3e-06
Identities = 37/98 (37%), Positives = 48/98 (48%)
Frame = -1
Query: 452 LEELLESFINRMESNYKIQDAAIKNLKSQFGQLAKQMAKIPQGKFSPNALILSKHENADD 511
LEE L F+ + SN+K ++A++NL+ Q GQLAKQ+ F N K E D
Sbjct: 321 LEETLTQFMKVIMSNHKSTESALENLEVQVGQLAKQITDKSSNSFMVNTEKNPK-EECKD 145
Query: 512 VTTRSGRVLHESKKQVEGEKNDKVEEKDEGVIKKRVSE 549
V TR SK+ VE E D V K + KK E
Sbjct: 144 VMTR-------SKRFVEAEDEDNVVSKKKFAEKKGTDE 52
>CF920352
Length = 571
Score = 49.3 bits (116), Expect = 7e-06
Identities = 40/111 (36%), Positives = 50/111 (45%)
Frame = -2
Query: 427 FQGQSSGLFQQVQEQVDVKDGGKKSLEELLESFINRMESNYKIQDAAIKNLKSQFGQLAK 486
F G G + Q+Q LEE L F+ SN K ++AIKNL+ Q GQLAK
Sbjct: 456 FNGDQGGSSIRPQQQGSSLYDRTTKLEETLAQFM*VSMSNQKSTESAIKNLEVQVGQLAK 277
Query: 487 QMAKIPQGKFSPNALILSKHENADDVTTRSGRVLHESKKQVEGEKNDKVEE 537
++A FS N K E V TRS H EG+ K+EE
Sbjct: 276 KLADRSSSSFSANTEKNPK-EECKAVMTRSKMTTHVD----EGKAEKKMEE 139
>BF595237
Length = 415
Score = 47.8 bits (112), Expect = 2e-05
Identities = 31/76 (40%), Positives = 41/76 (53%)
Frame = +3
Query: 424 NQGFQGQSSGLFQQVQEQVDVKDGGKKSLEELLESFINRMESNYKIQDAAIKNLKSQFGQ 483
NQ + Q S Q + V++ + K LEE L F+ SNY ++IKNL+ Q GQ
Sbjct: 156 NQFNKEQRSQPIQNSNQVVNLYEKTSK-LEETLNQFM*MFMSNYMSTKSSIKNLEIQMGQ 332
Query: 484 LAKQMAKIPQGKFSPN 499
LAKQMA+ P F N
Sbjct: 333 LAKQMAERPTNNFGAN 380
>TC232616 similar to GB|AAD25756.1|4587525|T5I8 Contains the PF|00650
CRAL/TRIO phosphatidyl-inositol-transfer protein domain.
ESTs gb|T76582, gb|N06574 and gb|Z25700 come from this
gene. {Arabidopsis thaliana;} , partial (48%)
Length = 1214
Score = 31.2 bits (69), Expect = 1.9
Identities = 19/40 (47%), Positives = 25/40 (62%)
Frame = -3
Query: 30 NFSSTTSTFSSAISTRCPDRFVFSSR*GRLSFF*DGCLFP 69
+FSST++ FSS+ S P F FSS G+ SFF +FP
Sbjct: 459 SFSSTSTLFSSSSSFSSPS-FFFSSFSGKASFFGSKRVFP 343
>TC232345
Length = 1083
Score = 31.2 bits (69), Expect = 1.9
Identities = 43/171 (25%), Positives = 68/171 (39%), Gaps = 2/171 (1%)
Frame = +2
Query: 487 QMAKIPQGKFSPNALILSKHENADDVTTRSGRVLHESKKQVEGEKNDKVEEKDEGVIKKR 546
Q+ KI K + IL K E A + RS +VL E+ VE K+
Sbjct: 263 QVDKIGSSKLT----ILKKLEEATEEVIRSKQVLTEALNSVESANR-----------KQH 397
Query: 547 VSEPVKARVLSTPSPELSKIPFPKALANKNLDKQFSKFLEVFKKLHINIPFSEALEQMLI 606
+E R + P +L P + K D+ + + +I + + +L+
Sbjct: 398 AAEEALRRWI--PQDDLIGQPVDNYVKPKKFDQSGNCHDCPLPDVTRSITMNNDPKPILM 571
Query: 607 YAKFMRDILSKKRKLSEVDETVMLTE--ECSAILQRKIPRKRRDPGSFTIP 655
+ MRD+LSKK+ E T + E E L + + R+D TIP
Sbjct: 572 SSVSMRDVLSKKQVPEEYTTTKEMEEHSERKVALSQMLRALRQDQTLPTIP 724
>TC218742 similar to GB|AAL57703.1|18086557|AY072008 AT5g04060/F8F6_270
{Arabidopsis thaliana;} , partial (30%)
Length = 939
Score = 30.0 bits (66), Expect = 4.3
Identities = 9/26 (34%), Positives = 21/26 (80%)
Frame = +2
Query: 710 IIEDVMVKVDKYVFPIDFVVLDMEED 735
++ED+M+++D+ + P+ F+++ EED
Sbjct: 509 LLEDIMLEMDRLIRPLGFIIIRDEED 586
>TC217912 weakly similar to UP|MFP1_ARATH (Q9LW85) MAR binding filament-like
protein 1, partial (16%)
Length = 756
Score = 29.6 bits (65), Expect = 5.6
Identities = 38/194 (19%), Positives = 78/194 (39%), Gaps = 4/194 (2%)
Frame = +2
Query: 346 IEKMAARAMNSENDRQTRRRMFEFEAYDKLLASNKQISEKVAEIQNHIKVTKLVGASVAK 405
++ + + + R++ R E EA L N+ E+Q + LV + +
Sbjct: 35 LQALEQQVSKDKESRKSLERDLE-EATKSLEEMNRNAVILSGELQ---RANSLVSSLEKE 202
Query: 406 VECVTRGGPNFSNSCTMTNQGFQGQSSGLFQQVQEQVDVKDGGKKSLEEL--LESFINRM 463
+ + + N N+C + + + + +E+ +++ GKK EEL + I R+
Sbjct: 203 KDVLIKSLTNQRNACKEAQDNIEDAHNLIMKLGKERENLEKKGKKFEEELASAKGEILRL 382
Query: 464 ESNYKIQDAAIKNLKSQFGQLAKQ--MAKIPQGKFSPNALILSKHENADDVTTRSGRVLH 521
+S A+ N G + K K+ K + N K E + VT + + +
Sbjct: 383 KSRINSSKVAVNN-----GPVQKDGGEKKVNPSKVAVNNEQAQKDEGENKVTVSARKTVR 547
Query: 522 ESKKQVEGEKNDKV 535
K + ++N +V
Sbjct: 548 RRKANPQ*QRN*RV 589
>TC219105 similar to GB|AAM10098.1|20148415|AY081536 endomembrane protein
EMP70 precusor isolog {Arabidopsis thaliana;} , partial
(59%)
Length = 1358
Score = 28.9 bits (63), Expect = 9.6
Identities = 15/74 (20%), Positives = 40/74 (53%)
Frame = -1
Query: 521 HESKKQVEGEKNDKVEEKDEGVIKKRVSEPVKARVLSTPSPELSKIPFPKALANKNLDKQ 580
H+S K+ + + K E ++K+R+ V+ + +TP ++ +IPF + + + +
Sbjct: 842 HQSSKEDDHHERVKNGEPMNLMLKERM---VQVDIKATPESDIGRIPFDR-ICHVQMFSY 675
Query: 581 FSKFLEVFKKLHIN 594
F + ++ ++++N
Sbjct: 674 FQRLWDILSEINMN 633
>TC216883 similar to GB|AAF86349.1|9255891|AF279129 FIN219 {Arabidopsis
thaliana;} , partial (46%)
Length = 1250
Score = 28.9 bits (63), Expect = 9.6
Identities = 17/59 (28%), Positives = 28/59 (46%)
Frame = +1
Query: 529 GEKNDKVEEKDEGVIKKRVSEPVKARVLSTPSPELSKIPFPKALANKNLDKQFSKFLEV 587
G++ + V G+ + R+ + VK +PEL I L N N+DK K L++
Sbjct: 271 GQEYEIVMTNPAGLYRYRLGDVVKVMGFHNSTPELKFIRRSSLLLNINIDKNTEKDLQL 447
>TC230567 similar to UP|Q9FGU0 (Q9FGU0) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K21L19, partial (9%)
Length = 1418
Score = 28.9 bits (63), Expect = 9.6
Identities = 33/110 (30%), Positives = 46/110 (41%), Gaps = 10/110 (9%)
Frame = +3
Query: 460 INRMESNYKIQDAAIKN--LKSQFGQLAKQMAKIP----QGKFSPNALILSKHENADDVT 513
+N+ S+ K +D N S+ G+ A + AK Q S A + EN D T
Sbjct: 255 VNKSSSSLKFKDIDKDNNDASSEAGKPAYEPAKTVDADNQHILSVEARDSADMENRDADT 434
Query: 514 TRSGR----VLHESKKQVEGEKNDKVEEKDEGVIKKRVSEPVKARVLSTP 559
SG + KK+ E K EK+ VIKK SEP+ + P
Sbjct: 435 KESGDRHLDTVERLKKRSERFKLPMPSEKEALVIKKLESEPLPSAKSENP 584
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.139 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,162,894
Number of Sequences: 63676
Number of extensions: 409428
Number of successful extensions: 2625
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 2590
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2619
length of query: 822
length of database: 12,639,632
effective HSP length: 105
effective length of query: 717
effective length of database: 5,953,652
effective search space: 4268768484
effective search space used: 4268768484
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0265.4