BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0260.11
(337 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE211208 44 1e-04
CO982036 40 0.002
TC228194 weakly similar to PIR|T04514|T04514 xyloglucan endo-1,4... 32 0.41
BF068614 similar to GP|27817858|db OJ1081_B12.7 {Oryza sativa (j... 32 0.41
BU550037 31 0.91
TC228193 weakly similar to PIR|T04514|T04514 xyloglucan endo-1,4... 30 1.2
TC221458 weakly similar to UP|Q940U2 (Q940U2) AT5g05220/K18I23_2... 30 1.5
BI316958 30 1.5
BU764568 30 1.5
TC231899 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Frag... 30 2.0
TC229375 28 5.9
BU548243 28 5.9
TC218991 similar to UP|Q8SMI2 (Q8SMI2) Acyl-ACP thioesterase, pa... 28 5.9
AW307380 28 5.9
>BE211208
Length = 413
Score = 43.9 bits (102), Expect = 1e-04
Identities = 35/106 (33%), Positives = 54/106 (50%), Gaps = 10/106 (9%)
Frame = +2
Query: 138 LHSVFPIKDLGELHYFL---MKHCKSASTLMIHD-FVCQMSVLFNLT*HFVKKRSILALL 193
L+S F +K LG+L YFL + H + S L+ ++C + L K S +
Sbjct: 104 LNSNFSLKQLGQLDYFLGIEVHHTPTGSVLLTQSKYICDL--LHKTDMAEAKPISSPMVT 277
Query: 194 SIQ*NSFGFSFNSHDSWLYKSIVGALH------PDIAFCVNKVCQF 233
+++ + G S D +Y+S+VGAL P+I+F NKVCQF
Sbjct: 278 NLRLSKNGDDLLS-DPTMYRSVVGALQYPTITRPEISFAANKVCQF 412
>CO982036
Length = 674
Score = 39.7 bits (91), Expect = 0.002
Identities = 38/108 (35%), Positives = 53/108 (48%), Gaps = 12/108 (11%)
Frame = -2
Query: 138 LHSVFPIKDLGELHYFLMKHCKSASTLMIHDFVCQMSVLFNLT*HFVKKRSILALLSIQ* 197
L+S FP+K LG+L YF+ KS L+ F + S+ F + F +K A
Sbjct: 577 LNSSFPLKLLGKLDYFVEIEVKSMPDLL---FSLRTSI-FEI---FCRKPR*QAQPISSP 419
Query: 198 NSFGFSFNSHDSWL------YKSIVGALH------PDIAFCVNKVCQF 233
+ + DS L Y+S+VGAL P+I+F VNKVCQF
Sbjct: 418 MTTTCKLSKSDSDLFSGPTFYRSVVGALQYTTVIRPEISFAVNKVCQF 275
>TC228194 weakly similar to PIR|T04514|T04514 xyloglucan
endo-1,4-beta-D-glucanase F16A16.40 - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (78%)
Length = 1053
Score = 32.0 bits (71), Expect = 0.41
Identities = 19/40 (47%), Positives = 26/40 (64%), Gaps = 2/40 (5%)
Frame = +3
Query: 126 LFFLTHLFFYLGL--HSVFPIKDLGELHYFLMKHCKSAST 163
LFFL+HLF + L HS PI+ L +HYF+ +C S S+
Sbjct: 858 LFFLSHLFVFQSL*FHSC*PIQ-LNNVHYFV--YCNSVSS 968
>BF068614 similar to GP|27817858|db OJ1081_B12.7 {Oryza sativa (japonica
cultivar-group)}, partial (3%)
Length = 413
Score = 32.0 bits (71), Expect = 0.41
Identities = 16/32 (50%), Positives = 20/32 (62%), Gaps = 6/32 (18%)
Frame = +1
Query: 208 DSWLYKSIVGALH------PDIAFCVNKVCQF 233
D LY S+VGAL P+ ++ VNKVCQF
Sbjct: 301 DPTLYGSVVGALQYATLTRPEFSYSVNKVCQF 396
>BU550037
Length = 728
Score = 30.8 bits (68), Expect = 0.91
Identities = 27/108 (25%), Positives = 50/108 (46%), Gaps = 8/108 (7%)
Frame = -3
Query: 134 FYLGLHSVFPIKDLGELHYFL-MKHCKSASTLMIHDFVCQMSVLFNLT*HFVKKRSILAL 192
++LG+ S F + DLG++ YFL M +S + I +L H + + I +
Sbjct: 375 YFLGMESEFEMTDLGQMKYFLGM*IFQSEDGIFISQKKYAWEILRKF--HMERCKPIATV 202
Query: 193 LSIQ*N-SFGFSFNSHDSWLYKSIVGAL------HPDIAFCVNKVCQF 233
L + S N D+ +Y+S++G+L P++ F + +F
Sbjct: 201 LVVNEKFSKDEEDNQGDASVYRSLIGSLLYLSATRPNLMFAATLLSRF 58
>TC228193 weakly similar to PIR|T04514|T04514 xyloglucan
endo-1,4-beta-D-glucanase F16A16.40 - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (40%)
Length = 771
Score = 30.4 bits (67), Expect = 1.2
Identities = 18/39 (46%), Positives = 25/39 (63%), Gaps = 2/39 (5%)
Frame = +2
Query: 126 LFFLTHLFFYLGL--HSVFPIKDLGELHYFLMKHCKSAS 162
LFFL+HLF + L HS PI+ L +HYF+ + S+S
Sbjct: 548 LFFLSHLFVFQSL*FHSC*PIQ-LNNVHYFVYCNIVSSS 661
>TC221458 weakly similar to UP|Q940U2 (Q940U2) AT5g05220/K18I23_2, partial
(25%)
Length = 545
Score = 30.0 bits (66), Expect = 1.5
Identities = 14/49 (28%), Positives = 26/49 (52%), Gaps = 6/49 (12%)
Frame = -3
Query: 125 PLFFLTHLFFYLGLHSVFPIKDLGELHY------FLMKHCKSASTLMIH 167
P + LF ++ H+ FP++ L LH+ F++K C S+ + +H
Sbjct: 459 PSVIIHRLFSHIFTHNSFPLQTLQHLHHPFSFSTFVLKLCHSSLLMCVH 313
>BI316958
Length = 408
Score = 30.0 bits (66), Expect = 1.5
Identities = 17/42 (40%), Positives = 26/42 (61%)
Frame = +3
Query: 152 YFLMKHCKSASTLMIHDFVCQMSVLFNLT*HFVKKRSILALL 193
YFL + ++ LM+ F C S FNL +FV+K+ I++LL
Sbjct: 114 YFLFE---ISTNLMVPLFKCLYSKYFNLNIYFVQKKLIISLL 230
>BU764568
Length = 420
Score = 30.0 bits (66), Expect = 1.5
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +3
Query: 236 LIFLTAKKVVVVS*SNTEAEYRSIAFDTI*PIWLQ 270
LI +KK VV+ S+ EAEYR++A T IWL+
Sbjct: 198 LISWKSKKQSVVAKSSAEAEYRAMALVTCELIWLK 302
>TC231899 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Fragment),
partial (30%)
Length = 687
Score = 29.6 bits (65), Expect = 2.0
Identities = 15/35 (42%), Positives = 23/35 (64%)
Frame = +2
Query: 236 LIFLTAKKVVVVS*SNTEAEYRSIAFDTI*PIWLQ 270
L+ +KK VV+ S+ EAEYRS+A T +W++
Sbjct: 104 LVSWKSKKQTVVARSSAEAEYRSMAMVTCELMWIK 208
>TC229375
Length = 1226
Score = 28.1 bits (61), Expect = 5.9
Identities = 13/44 (29%), Positives = 23/44 (51%)
Frame = +3
Query: 136 LGLHSVFPIKDLGELHYFLMKHCKSASTLMIHDFVCQMSVLFNL 179
+G ++ I+D+ H FLM+H K+ + C + +L NL
Sbjct: 813 IGSIALVHIEDIYSAHIFLMEHSKAEGRYICSSQSCPLDMLANL 944
>BU548243
Length = 599
Score = 28.1 bits (61), Expect = 5.9
Identities = 16/35 (45%), Positives = 22/35 (62%)
Frame = -1
Query: 236 LIFLTAKKVVVVS*SNTEAEYRSIAFDTI*PIWLQ 270
LI ++K V + S+TEAEYRSIA + W+Q
Sbjct: 515 LISWWSRKQQVTAQSSTEAEYRSIAQTSAELTWIQ 411
>TC218991 similar to UP|Q8SMI2 (Q8SMI2) Acyl-ACP thioesterase, partial (17%)
Length = 785
Score = 28.1 bits (61), Expect = 5.9
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = -3
Query: 48 KRSSYEL*KAFYG*QWDHHGWFDKHNFISTLDKKKLYISSF 88
+ ++ E K +G W H WF +F+S +Y+ SF
Sbjct: 474 RETTQERQKQNHGFGWKHT*WFSSWSFLSPFRSSPVYLKSF 352
>AW307380
Length = 422
Score = 28.1 bits (61), Expect = 5.9
Identities = 13/44 (29%), Positives = 23/44 (51%)
Frame = +2
Query: 136 LGLHSVFPIKDLGELHYFLMKHCKSASTLMIHDFVCQMSVLFNL 179
+G ++ I+D+ H FLM+H K+ + C + +L NL
Sbjct: 17 MGSIALVHIEDICSAHIFLMEHSKAEGRYICSSQSCPLDMLANL 148
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.364 0.164 0.596
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,429,181
Number of Sequences: 63676
Number of extensions: 284587
Number of successful extensions: 4365
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 3238
Number of HSP's successfully gapped in prelim test: 126
Number of HSP's that attempted gapping in prelim test: 1062
Number of HSP's gapped (non-prelim): 3445
length of query: 337
length of database: 12,639,632
effective HSP length: 98
effective length of query: 239
effective length of database: 6,399,384
effective search space: 1529452776
effective search space used: 1529452776
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.5 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0260.11