BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0252c.1
(325 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ273711 84 1e-16
CF922015 61 8e-10
TC226202 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp... 37 0.012
TC226200 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp... 37 0.012
TC226201 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp... 37 0.012
TC223754 similar to UP|Q86EQ4 (Q86EQ4) Clone ZZD1536 mRNA sequen... 37 0.016
TC205091 weakly similar to UP|Q6TXI7 (Q6TXI7) LRRGT00012, partia... 35 0.061
TC216231 similar to UP|Q41188 (Q41188) Glycine-rich protein 2 (G... 34 0.079
TC206177 similar to UP|Q41188 (Q41188) Glycine-rich protein 2 (G... 33 0.23
TC222094 33 0.23
TC206176 weakly similar to UP|Q8LPA7 (Q8LPA7) Cold shock protein... 33 0.23
TC219583 weakly similar to UP|GRP2_NICSY (P27484) Glycine-rich p... 33 0.23
TC233976 32 0.30
TC225188 similar to UP|Q9FYB7 (Q9FYB7) Splicing factor-like prot... 32 0.30
TC232867 similar to UP|Q9MA68 (Q9MA68) F2J6.13 protein, partial ... 32 0.30
TC212368 weakly similar to UP|Q8W2K5 (Q8W2K5) Phragmoplastin-int... 32 0.39
AI416791 32 0.39
TC227588 similar to PIR|T00837|T00837 glycine-rich protein T13L1... 31 0.67
CO979236 31 0.67
TC227589 31 0.67
>BQ273711
Length = 409
Score = 83.6 bits (205), Expect = 1e-16
Identities = 41/101 (40%), Positives = 60/101 (58%)
Frame = -2
Query: 55 ELHLRQREASLDQNRGLNNFIRQNPPKFTGGTDPDEADLWIQEIEKIFEVLQTSEGAKVG 114
E +++R+ + RGL F + +PPKF+G DP+ A LW+ E EKIFE + E KV
Sbjct: 303 ETLVQERDVEPAEYRGLMAFRKNHPPKFSGDYDPEGARLWLAETEKIFEAMGCLEEHKVL 124
Query: 115 LATYLLLGDAEYWWRGARGMMEANHVEVNWNSFRAAFLEKY 155
AT++L G+AE WW+ + A + WN+F+ FLE Y
Sbjct: 123 YATFMLQGEAENWWKFVKPSFVAPGGVIPWNAFKDKFLENY 1
>CF922015
Length = 172
Score = 60.8 bits (146), Expect = 8e-10
Identities = 27/55 (49%), Positives = 38/55 (69%)
Frame = -3
Query: 70 GLNNFIRQNPPKFTGGTDPDEADLWIQEIEKIFEVLQTSEGAKVGLATYLLLGDA 124
GL+ F R NPP F GG DP+ A+ W++EIEKIF V++ + KV AT++L +A
Sbjct: 167 GLDRFQRNNPPTFKGGYDPEGAEAWLREIEKIFRVMECQDHQKVLFATHMLADEA 3
>TC226202 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp22 splicing
factor), partial (72%)
Length = 1003
Score = 37.0 bits (84), Expect = 0.012
Identities = 11/27 (40%), Positives = 20/27 (73%)
Frame = +3
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKC 321
GG G ++G ++ C++CGE GH++ +C
Sbjct: 795 GGRGGRSGGSDLKCYECGEPGHFAREC 875
>TC226200 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp22 splicing
factor), partial (89%)
Length = 936
Score = 37.0 bits (84), Expect = 0.012
Identities = 11/27 (40%), Positives = 20/27 (73%)
Frame = +2
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKC 321
GG G ++G ++ C++CGE GH++ +C
Sbjct: 299 GGRGGRSGGSDLKCYECGEPGHFAREC 379
>TC226201 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp22 splicing
factor), partial (72%)
Length = 820
Score = 37.0 bits (84), Expect = 0.012
Identities = 11/27 (40%), Positives = 20/27 (73%)
Frame = +1
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKC 321
GG G ++G ++ C++CGE GH++ +C
Sbjct: 208 GGRGGRSGGSDLKCYECGEPGHFAREC 288
>TC223754 similar to UP|Q86EQ4 (Q86EQ4) Clone ZZD1536 mRNA sequence, partial
(22%)
Length = 742
Score = 36.6 bits (83), Expect = 0.016
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = +2
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKC 321
GG GS G + TCF CG+ GH++ +C
Sbjct: 383 GGGGSGGGGGKSTCFNCGKPGHFAREC 463
Score = 32.0 bits (71), Expect = 0.39
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +2
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKC 321
GG GS G CF+CGE+GH + C
Sbjct: 293 GGGGSGGG-----CFRCGEVGHLARDC 358
Score = 31.2 bits (69), Expect = 0.67
Identities = 15/41 (36%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = +2
Query: 286 LYRPTIAAAGGA-GSQAGSREITCFKCGEIGHYSTKCPKGK 325
L R + GG+ G G +CF+CG GH + C GK
Sbjct: 161 LARDCVRGGGGSVGIGGGGGGGSCFRCGGFGHMARDCATGK 283
Score = 30.8 bits (68), Expect = 0.87
Identities = 14/37 (37%), Positives = 18/37 (47%)
Frame = +2
Query: 288 RPTIAAAGGAGSQAGSREITCFKCGEIGHYSTKCPKG 324
R + + GG GS G CF CG GH + C +G
Sbjct: 89 RSSNSGGGGGGSGGG-----CFNCGGFGHLARDCVRG 184
>TC205091 weakly similar to UP|Q6TXI7 (Q6TXI7) LRRGT00012, partial (5%)
Length = 1360
Score = 34.7 bits (78), Expect = 0.061
Identities = 20/45 (44%), Positives = 24/45 (52%), Gaps = 1/45 (2%)
Frame = +1
Query: 85 GTDPDEADL-WIQEIEKIFEVLQTSEGAKVGLATYLLLGDAEYWW 128
G D EA L W ++E++F SE KV LAT G A YWW
Sbjct: 1153 GKDNVEAYLDWEMKVEQLFACHHISEERKVPLATLSFQGYALYWW 1287
>TC216231 similar to UP|Q41188 (Q41188) Glycine-rich protein 2 (GRP2)
(AT4g38680/F20M13_240), partial (42%)
Length = 891
Score = 34.3 bits (77), Expect = 0.079
Identities = 24/77 (31%), Positives = 28/77 (36%)
Frame = +3
Query: 248 RAGTGGPMRTSSRSYQGKGKLQRKKPYQRPTGEGFTPGLYRPTIAAAGGAGSQAGSREIT 307
R G GG R Y G G G+ G Y GG G G
Sbjct: 102 RGGYGGGGRGGRGGYGGGGG-------------GYGGGGYGGGGGYGGGGGGGGGG---- 230
Query: 308 CFKCGEIGHYSTKCPKG 324
C+KCGE GH + C +G
Sbjct: 231 CYKCGETGHIARDCSQG 281
Score = 32.7 bits (73), Expect = 0.23
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = +3
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKCP 322
GG G GS C+ CGE GH++ CP
Sbjct: 303 GGGGGGGGS----CYNCGESGHFARDCP 374
>TC206177 similar to UP|Q41188 (Q41188) Glycine-rich protein 2 (GRP2)
(AT4g38680/F20M13_240), partial (22%)
Length = 406
Score = 32.7 bits (73), Expect = 0.23
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = +1
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKCP 322
GG G GS C+ CGE GH++ CP
Sbjct: 79 GGGGGGGGS----CYSCGESGHFARDCP 150
>TC222094
Length = 984
Score = 32.7 bits (73), Expect = 0.23
Identities = 20/65 (30%), Positives = 32/65 (48%)
Frame = -2
Query: 115 LATYLLLGDAEYWWRGARGMMEANHVEVNWNSFRAAFLEKYFPDSARDERESQFLTLRQG 174
L + L G+A W R +G N++ WN FL+KYFP ES+ + + G
Sbjct: 614 LFCFSLAGEARTWLRSFKG----NNLRT-WNEXXEKFLKKYFP-------ESKTIXXKGG 471
Query: 175 SMTIP 179
++ +P
Sbjct: 470 NLILP 456
>TC206176 weakly similar to UP|Q8LPA7 (Q8LPA7) Cold shock protein-1, partial
(77%)
Length = 883
Score = 32.7 bits (73), Expect = 0.23
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = +3
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKCP 322
GG G GS C+ CGE GH++ CP
Sbjct: 564 GGGGGGGGS----CYSCGESGHFARDCP 635
Score = 28.1 bits (61), Expect = 5.7
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +3
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKCPKG 324
GG G G+ C+ CGE GH + C G
Sbjct: 408 GGRGGGGGA----CYNCGESGHLARDCSGG 485
>TC219583 weakly similar to UP|GRP2_NICSY (P27484) Glycine-rich protein 2,
partial (45%)
Length = 995
Score = 32.7 bits (73), Expect = 0.23
Identities = 13/28 (46%), Positives = 16/28 (56%)
Frame = +2
Query: 295 GGAGSQAGSREITCFKCGEIGHYSTKCP 322
GG G G CF CGE GH++ +CP
Sbjct: 518 GGGGGGGGG----CFNCGEEGHFARECP 589
Score = 29.6 bits (65), Expect = 1.9
Identities = 16/47 (34%), Positives = 20/47 (42%)
Frame = +2
Query: 279 GEGFTPGLYRPTIAAAGGAGSQAGSREITCFKCGEIGHYSTKCPKGK 325
G G G Y G G + G E C+ CG IGH + C G+
Sbjct: 344 GRGRGGGRYGGGEGRGRGFGRRGGGPE--CYNCGRIGHLARDCYHGQ 478
>TC233976
Length = 763
Score = 32.3 bits (72), Expect = 0.30
Identities = 24/80 (30%), Positives = 28/80 (35%), Gaps = 10/80 (12%)
Frame = +1
Query: 253 GPMRTSSRSYQGKGKLQRKKPYQRPTGEGFTPGLYRPTIAAA----------GGAGSQAG 302
G RTS + G Q +P G G G P I G +
Sbjct: 145 GGQRTSGGHHLAGGSSQLVNRVSQPAGRG---GSGAPAIVTTPLRCRKCGRLGHNAHECT 315
Query: 303 SREITCFKCGEIGHYSTKCP 322
RE+TCF GH ST CP
Sbjct: 316 YREVTCFNYQGKGHLSTNCP 375
>TC225188 similar to UP|Q9FYB7 (Q9FYB7) Splicing factor-like protein, partial
(62%)
Length = 1131
Score = 32.3 bits (72), Expect = 0.30
Identities = 20/70 (28%), Positives = 27/70 (38%)
Frame = +1
Query: 252 GGPMRTSSRSYQGKGKLQRKKPYQRPTGEGFTPGLYRPTIAAAGGAGSQAGSREITCFKC 311
GGP + R Y G+G P G G + I +AG + C++C
Sbjct: 298 GGPRGSRDREYMGRGP---------PPGSG---RCFNCGIDGHWARDCKAGDWKNKCYRC 441
Query: 312 GEIGHYSTKC 321
GE GH C
Sbjct: 442 GERGHIEKNC 471
>TC232867 similar to UP|Q9MA68 (Q9MA68) F2J6.13 protein, partial (3%)
Length = 1144
Score = 32.3 bits (72), Expect = 0.30
Identities = 29/121 (23%), Positives = 54/121 (43%), Gaps = 8/121 (6%)
Frame = -3
Query: 108 SEGAKVGLATYLLLGDAEYWWRGARGMMEANHVEVNWNSFRAAFLEKYFPDSARDERESQ 167
++ ++ L ++ L G+A+ W +G N ++ +W+ FL+KYFP+S E ++
Sbjct: 695 ADAIRLSLLSFSLSGEAKRWLHSFKG----NSLK-SWDEVVEKFLKKYFPESKTAEGKAA 531
Query: 168 FLTLRQGSMTIPEYAAKLESLAKHFRFFRDQV--------DEPYMCKRFVRGLRADIEDS 219
+ Q P+ ESL++ FR + EP F+ LR + +
Sbjct: 530 ISSFHQ----FPD-----ESLSEALERFRGLLRKTPTHGFSEPIQLNIFIDELRPESKQL 378
Query: 220 V 220
V
Sbjct: 377 V 375
>TC212368 weakly similar to UP|Q8W2K5 (Q8W2K5) Phragmoplastin-interacting
protein PHIP1, partial (4%)
Length = 480
Score = 32.0 bits (71), Expect = 0.39
Identities = 16/34 (47%), Positives = 18/34 (52%), Gaps = 3/34 (8%)
Frame = +1
Query: 293 AAGGAGSQAGS---REITCFKCGEIGHYSTKCPK 323
A G S GS R TC++CGE GH CPK
Sbjct: 64 ADGDKPSSTGSGKMRRRTCYECGEKGHTFAACPK 165
>AI416791
Length = 420
Score = 32.0 bits (71), Expect = 0.39
Identities = 33/126 (26%), Positives = 54/126 (42%), Gaps = 7/126 (5%)
Frame = -3
Query: 141 EVNWNSFRAAFLEKYFPDSARDERESQFLTLRQGSMTIPEYAAKLESLAKHFRFFRDQVD 200
++ W S + A LE+Y D E ++GS + +Y + E L ++
Sbjct: 382 DLTWESLKIALLERYGGHGDGDVYEQLTELKQEGS--VEDYITEFEYLIAQIPRLPEKQF 209
Query: 201 EPYMCKRFVRGLRADIEDSVRPLGIM----RFQAL-VEKATEVELM--KNRRMDRAGTGG 253
+ Y F+ GL+++I VR L M R + L V +A E E+ R G
Sbjct: 208 QGY----FLHGLQSEIRGRVRTLATMGEMSRTKLLQVTRAVEKEVKGGNGSGFGRGPKNG 41
Query: 254 PMRTSS 259
P R++S
Sbjct: 40 PYRSNS 23
>TC227588 similar to PIR|T00837|T00837 glycine-rich protein T13L16.11 -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(10%)
Length = 1300
Score = 31.2 bits (69), Expect = 0.67
Identities = 10/21 (47%), Positives = 16/21 (75%)
Frame = +1
Query: 301 AGSREITCFKCGEIGHYSTKC 321
+G+ +CFKCGE GH++ +C
Sbjct: 484 SGATPSSCFKCGEEGHFAREC 546
Score = 30.0 bits (66), Expect = 1.5
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +1
Query: 305 EITCFKCGEIGHYSTKCPK 323
EI+C+KCG++GH C +
Sbjct: 409 EISCYKCGQLGHTGLACSR 465
>CO979236
Length = 729
Score = 31.2 bits (69), Expect = 0.67
Identities = 36/178 (20%), Positives = 73/178 (40%), Gaps = 13/178 (7%)
Frame = -1
Query: 102 FEVLQTSEGAKVGLATYLLLGDAEYWWRGARGMMEANHVEVNWNSFRAAFLEKYFPDSAR 161
F+V +TS+ KV LA + +++ ++ + + W + +++Y
Sbjct: 729 FQVQETSDEVKVRLAQISMENGTVHFFN----LLLLENENLTWEELKYELMQRYAGKGTA 562
Query: 162 DERESQFLTLRQGSMTIPEYAAKLESLA---------KHFRFFRDQVDEPYMCKRFVRGL 212
E+ L+ Q + +I +Y + E L ++F F + ++ RG
Sbjct: 561 YEQ----LSALQQTRSIDDYIQRFECLIAQIPKLQDEQYFACFTHGLKSLHVANLLTRGR 394
Query: 213 RADIEDSVRPLGIMRFQALVEKATEVELMKNRRMDRAGTGGPM----RTSSRSYQGKG 266
+I +V+ + +A V + M++ +G P+ RT SR+Y GKG
Sbjct: 393 LMNIARAVKMEMTSKNRAWVGRGDTC--MESGTRTASGQRNPLVATGRTGSRNYGGKG 226
>TC227589
Length = 547
Score = 31.2 bits (69), Expect = 0.67
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +2
Query: 305 EITCFKCGEIGHYSTKCPK 323
EI+C+KCG++GH C K
Sbjct: 395 EISCYKCGQLGHTGLACSK 451
Score = 27.3 bits (59), Expect = 9.7
Identities = 8/15 (53%), Positives = 12/15 (79%)
Frame = +2
Query: 307 TCFKCGEIGHYSTKC 321
+C KCGE GH++ +C
Sbjct: 488 SCCKCGEAGHFAQEC 532
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.136 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,153,830
Number of Sequences: 63676
Number of extensions: 131044
Number of successful extensions: 758
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 721
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 755
length of query: 325
length of database: 12,639,632
effective HSP length: 97
effective length of query: 228
effective length of database: 6,463,060
effective search space: 1473577680
effective search space used: 1473577680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0252c.1