BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0227.9
(273 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208526 similar to UP|Q6YW97 (Q6YW97) MAP3K protein kinase-like... 245 1e-65
CO985741 138 3e-33
AW164281 similar to PIR|T11688|T11 phosphoinositide-specific pho... 35 0.047
TC230000 UP|Q43439 (Q43439) Phosphatidylinositol-specific phosph... 33 0.18
TC230320 similar to UP|Q9FZ09 (Q9FZ09) Patatin-like protein 1, p... 32 0.40
TC233681 UP|Q43442 (Q43442) Phosphoinositide-specific phospholip... 30 0.90
TC218867 homologue to UP|Q43444 (Q43444) Phosphoinositide-specif... 29 2.0
CO983198 29 2.6
TC203547 weakly similar to UP|Q75IX4 (Q75IX4) Expressed protein,... 28 3.4
BE058237 28 4.4
TC217247 UP|Q8S902 (Q8S902) Syringolide-induced protein 19-1-5, ... 28 5.8
TC218378 27 7.6
TC204297 similar to UP|Q9LJE5 (Q9LJE5) Gb|AAD10646.1 (AT3g13460/... 27 7.6
BE330663 27 7.6
TC225880 UP|O49154 (O49154) Ferredoxin-dependent glutamate synth... 27 7.6
TC211660 similar to UP|Q711G4 (Q711G4) Cullin 3B (Fragment), par... 27 9.9
>TC208526 similar to UP|Q6YW97 (Q6YW97) MAP3K protein kinase-like protein,
partial (59%)
Length = 1214
Score = 245 bits (626), Expect = 1e-65
Identities = 115/218 (52%), Positives = 158/218 (71%), Gaps = 3/218 (1%)
Frame = +2
Query: 53 LDMWDYEDTIWLC---RGPCTKYTTFQPALNVLKEVRVFLVTHPTEIITIFIDDHVTSGN 109
LDM+D+++ IWLC RG C +T FQPA+N LKEV FL +PTEI+TI I+D+V +
Sbjct: 23 LDMYDFQNDIWLCHSFRGQCYNFTAFQPAVNTLKEVEAFLTENPTEIVTIIIEDYVHTPK 202
Query: 110 GVNKVFDKARLRKFWFPVSKMPKNGSDWPTVKTMIRKNYRLIVFTSNASREASEGIAYEW 169
G+ VF A L K+WFPVSKMPK G DWPTV M++ N+RL+VFTS+AS+EA EGIAY+W
Sbjct: 203 GLTNVFTSAGLDKYWFPVSKMPKKGDDWPTVTEMVQANHRLVVFTSDASKEAGEGIAYQW 382
Query: 170 NYVVESQFGNVGIKGGSCQNRPESLPMNNATKSLVLMNYFRNVRNHDDEACRDNSSPLIA 229
++VE++ G+ G++ GSC +R ES +N+ + SL LMNYF D +C+++S+PL
Sbjct: 383 KHMVENESGDPGVQQGSCPHRKESKALNSKSHSLFLMNYFPTYPVEAD-SCKEHSAPLAE 559
Query: 230 MMHVCFRAAGNRWPNFIAVDFYKRGDGGGAPEALDLAN 267
M++ C++AAGN PNFIAV+FY R DGGG + +D N
Sbjct: 560 MVNTCYKAAGNLMPNFIAVNFYMRSDGGGVFDIVDKMN 673
>CO985741
Length = 761
Score = 138 bits (347), Expect = 3e-33
Identities = 65/108 (60%), Positives = 82/108 (75%)
Frame = -1
Query: 163 EGIAYEWNYVVESQFGNVGIKGGSCQNRPESLPMNNATKSLVLMNYFRNVRNHDDEACRD 222
EGIAY+W YVVE+Q+G+ G+K GSC +R ES MN ++SLVL+NYF + N +AC D
Sbjct: 761 EGIAYQWTYVVENQYGDDGMKAGSCPSRAESPAMNTKSRSLVLVNYFHSAPNRS-QACAD 585
Query: 223 NSSPLIAMMHVCFRAAGNRWPNFIAVDFYKRGDGGGAPEALDLANRNL 270
NS+PL+ MM C AAGNRW NFIAVD+Y+R DGGGAP A+D AN +L
Sbjct: 584 NSAPLLDMMKTCHEAAGNRWANFIAVDYYQRSDGGGAPLAVDEANGHL 441
>AW164281 similar to PIR|T11688|T11 phosphoinositide-specific phospholipase C
(EC 3.1.4.-) - cowpea, partial (21%)
Length = 433
Score = 34.7 bits (78), Expect = 0.047
Identities = 35/117 (29%), Positives = 53/117 (44%), Gaps = 11/117 (9%)
Frame = +2
Query: 1 MELPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMW--DY 58
M P + Y T HNS+ TG+ L+ + I L+ GVR + LD+W
Sbjct: 68 MTAPLSHYFIYTGHNSYL-------TGNQ-LSSDCSDVPIIKALQRGVRVIELDLWPNSD 223
Query: 59 EDTIWLCRGPC-------TKYTTFQPALNVLKEVR--VFLVTHPTEIITIFIDDHVT 106
ED I + G C + TT + LK ++ F+ +H IIT ++DH+T
Sbjct: 224 EDDIEVVHGRC*LILSSISTLTTPVSLIQCLKSIKEYAFVKSHYPLIIT--LEDHLT 388
>TC230000 UP|Q43439 (Q43439) Phosphatidylinositol-specific phospholipase C ,
complete
Length = 2116
Score = 32.7 bits (73), Expect = 0.18
Identities = 30/108 (27%), Positives = 48/108 (43%), Gaps = 2/108 (1%)
Frame = +1
Query: 1 MELPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMWD--Y 58
M LP + Y T HNS+ TG+ L+ + I + LK GVR + LD+W
Sbjct: 439 MTLPLSHYFIYTGHNSYL-------TGNQ-LSSDCSDVPIINALKRGVRVIELDIWPNAS 594
Query: 59 EDTIWLCRGPCTKYTTFQPALNVLKEVRVFLVTHPTEIITIFIDDHVT 106
+D I + G TT + L+ ++ + I ++DH+T
Sbjct: 595 KDNIDVLHG--RTLTTPVELIRCLRSIKDHAFVASEYPVVITLEDHLT 732
>TC230320 similar to UP|Q9FZ09 (Q9FZ09) Patatin-like protein 1, partial (89%)
Length = 1422
Score = 31.6 bits (70), Expect = 0.40
Identities = 16/52 (30%), Positives = 25/52 (47%)
Frame = +2
Query: 156 NASREASEGIAYEWNYVVESQFGNVGIKGGSCQNRPESLPMNNATKSLVLMN 207
N + + S+G +E+N I GG C N P + MN TK +++ N
Sbjct: 554 NFNNQDSKGNVHEFNL----------IDGGVCANNPTLVAMNEVTKQIIMQN 679
>TC233681 UP|Q43442 (Q43442) Phosphoinositide-specific phospholipase C P12 ,
complete
Length = 1880
Score = 30.4 bits (67), Expect = 0.90
Identities = 28/108 (25%), Positives = 46/108 (41%), Gaps = 2/108 (1%)
Frame = +1
Query: 1 MELPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMW--DY 58
M P + Y T HNS+ TG+ L+ + I L+ GVR + LD+W
Sbjct: 316 MNAPLSHYFIYTGHNSYL-------TGNQ-LSSDCSDVPIIKALQRGVRVIELDLWPNST 471
Query: 59 EDTIWLCRGPCTKYTTFQPALNVLKEVRVFLVTHPTEIITIFIDDHVT 106
+D I + G T + LK ++ + + I ++DH+T
Sbjct: 472 KDDIDVVHG--RTLTAPVSLIQCLKSIKEYAFVKSDYPVIITLEDHLT 609
>TC218867 homologue to UP|Q43444 (Q43444) Phosphoinositide-specific
phospholipase C P25 , complete
Length = 2341
Score = 29.3 bits (64), Expect = 2.0
Identities = 27/108 (25%), Positives = 47/108 (43%), Gaps = 2/108 (1%)
Frame = +3
Query: 1 MELPFNEYSWLTTHNSFAAKGVNWSTGSPVLAFTNQEDSITDQLKNGVRGLMLDMWDYE- 59
M P + Y T HNS+ TG+ L+ + I + L+ GVR + LD+W E
Sbjct: 342 MSSPLSHYFIYTGHNSYL-------TGNQ-LSSDCSDIPIINALQKGVRVIELDIWPNES 497
Query: 60 -DTIWLCRGPCTKYTTFQPALNVLKEVRVFLVTHPTEIITIFIDDHVT 106
D + + G T+ + L+ ++ + I ++DH+T
Sbjct: 498 KDNVDVLHG--RTLTSPVALIKCLRSIKQHAFVASEYPVVITLEDHLT 635
>CO983198
Length = 736
Score = 28.9 bits (63), Expect = 2.6
Identities = 12/37 (32%), Positives = 23/37 (61%)
Frame = -1
Query: 218 EACRDNSSPLIAMMHVCFRAAGNRWPNFIAVDFYKRG 254
++C+++ PL M++ C++AAGN +AV + G
Sbjct: 166 DSCKEHLVPLADMVNTCYKAAGNHVLPNLAVKRHVNG 56
>TC203547 weakly similar to UP|Q75IX4 (Q75IX4) Expressed protein, partial
(6%)
Length = 302
Score = 28.5 bits (62), Expect = 3.4
Identities = 9/23 (39%), Positives = 18/23 (78%)
Frame = +3
Query: 218 EACRDNSSPLIAMMHVCFRAAGN 240
++C+++ PL M++ C++AAGN
Sbjct: 39 DSCKEHLVPLADMVNTCYKAAGN 107
>BE058237
Length = 413
Score = 28.1 bits (61), Expect = 4.4
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -2
Query: 222 DNSSPLIAMMHVCFRAAGNRWPNFIAVDFYKRG 254
+NS LI M+ C A N++AVD+YK G
Sbjct: 376 NNSGGLIDMLQTCHGA------NYVAVDYYKAG 296
>TC217247 UP|Q8S902 (Q8S902) Syringolide-induced protein 19-1-5, complete
Length = 1205
Score = 27.7 bits (60), Expect = 5.8
Identities = 18/72 (25%), Positives = 25/72 (34%), Gaps = 8/72 (11%)
Frame = +2
Query: 8 YSWLTTHNSFAAKGVNWST--------GSPVLAFTNQEDSITDQLKNGVRGLMLDMWDYE 59
Y W F + W+ GSP+ F N E KN + +W+ +
Sbjct: 497 YLWFDPTADFHTYSILWNPQRIVFSVDGSPIREFKNMESKGVPFPKNQAMRIYSSLWNAD 676
Query: 60 DTIWLCRGPCTK 71
D W RG K
Sbjct: 677 D--WATRGGLVK 706
>TC218378
Length = 805
Score = 27.3 bits (59), Expect = 7.6
Identities = 13/30 (43%), Positives = 22/30 (73%)
Frame = -3
Query: 146 KNYRLIVFTSNASREASEGIAYEWNYVVES 175
K+ ++ +FT++ASR S+GI +WNY +S
Sbjct: 761 KHGKVSIFTNSASRVFSQGI-QQWNYKEKS 675
>TC204297 similar to UP|Q9LJE5 (Q9LJE5) Gb|AAD10646.1 (AT3g13460/MRP15_10),
partial (48%)
Length = 2995
Score = 27.3 bits (59), Expect = 7.6
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +3
Query: 89 LVTHPTEIITIFIDDHVTSGNGVNKVFDKARLR 121
+VTHP IIT+ + + VTSG + + + LR
Sbjct: 444 MVTHPLPIITVVMMELVTSGMSIQDM*IRKELR 542
>BE330663
Length = 385
Score = 27.3 bits (59), Expect = 7.6
Identities = 12/41 (29%), Positives = 21/41 (50%), Gaps = 3/41 (7%)
Frame = -1
Query: 57 DYEDTIWLCRGPCTKYTTFQPALNVLKEVRVFL---VTHPT 94
++ D WL P TTFQ +N++ + ++ + HPT
Sbjct: 322 NHSDHAWLIDIPTQYQTTFQSHINIISSILCYIGDAIFHPT 200
>TC225880 UP|O49154 (O49154) Ferredoxin-dependent glutamate synthase
(Fragment) , partial (55%)
Length = 2224
Score = 27.3 bits (59), Expect = 7.6
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +1
Query: 56 WDYEDTIWLCRGPCTKYTTFQ 76
W++ IW C+G C YT F+
Sbjct: 232 WNWHCCIWGCKGQC*HYTDFR 294
>TC211660 similar to UP|Q711G4 (Q711G4) Cullin 3B (Fragment), partial (29%)
Length = 747
Score = 26.9 bits (58), Expect = 9.9
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 74 TFQPALNVLKEVRVFLVTHPTEIITIFIDDHVTSG 108
TFQ ALN E + L E I++F+DD + G
Sbjct: 76 TFQNALNSSFEYFINLNARSPEFISLFVDDKLRRG 180
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.136 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,257,410
Number of Sequences: 63676
Number of extensions: 175458
Number of successful extensions: 773
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 769
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 770
length of query: 273
length of database: 12,639,632
effective HSP length: 96
effective length of query: 177
effective length of database: 6,526,736
effective search space: 1155232272
effective search space used: 1155232272
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0227.9