BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0223.4
(336 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205504 similar to UP|Q9LM78 (Q9LM78) F2D10.25, partial (20%) 30 2.0
TC220614 similar to UP|Q6R3R1 (Q6R3R1) CONSTANS-like protein CO2... 28 4.5
TC206288 homologue to UP|HMGA_SOYBN (Q00423) HMG-Y related prote... 28 4.5
TC210087 28 4.5
TC217787 weakly similar to UP|Q76EK9 (Q76EK9) Steroid 5alpha-red... 28 4.5
TC205914 weakly similar to UP|NAM8_YEAST (Q00539) NAM8 protein, ... 28 4.5
TC206289 UP|HMGB_SOYBN (Q10370) HMG-Y related protein B (SB16B p... 28 4.5
TC218189 similar to UP|Q94A38 (Q94A38) AT5g46250/MPL12_3, partia... 28 4.5
TC231948 similar to UP|Q6NNI3 (Q6NNI3) At5g28490, partial (69%) 28 5.9
TC219720 similar to UP|Q6S7C3 (Q6S7C3) TFIIE-alpha 2 (Fragment),... 28 5.9
TC224292 28 5.9
AW830527 weakly similar to SP|Q91062|VIT_ Vitellogenin precursor... 28 5.9
CA935575 28 5.9
TC228275 weakly similar to UP|Q9M3D0 (Q9M3D0) Lipase-like protei... 28 7.7
TC207130 similar to GB|AAH34435.1|21707915|BC034435 ZC3HDC3 prot... 28 7.7
TC217093 28 7.7
BQ080717 28 7.7
TC218620 similar to UP|Q8GUA8 (Q8GUA8) Ornithine aminotransferas... 27 10.0
TC229591 UP|O82585 (O82585) NADP-dependent isocitrate dehydrogen... 27 10.0
>TC205504 similar to UP|Q9LM78 (Q9LM78) F2D10.25, partial (20%)
Length = 1593
Score = 29.6 bits (65), Expect = 2.0
Identities = 18/55 (32%), Positives = 32/55 (57%), Gaps = 5/55 (9%)
Frame = +2
Query: 261 ANFREIRNRELDLQQADQQLKQSE-----LQLRQEELKFKKAENFRAYMDILNKN 310
A FR+I++R+++LQQA ++ Q LQ+R E ++ E F+A + K+
Sbjct: 26 AKFRDIQDRKVELQQAIVKMVQGGSADGILQVRAERIQSDLEELFKALAERCKKH 190
>TC220614 similar to UP|Q6R3R1 (Q6R3R1) CONSTANS-like protein CO2, partial
(33%)
Length = 640
Score = 28.5 bits (62), Expect = 4.5
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +2
Query: 12 APPTGSTGSKVSDTQCEATPDDTQHEGLDDIDLEDEDQSS 51
A P S +V A P + ++ +DD DL+D+D+++
Sbjct: 347 ANPLASRHHRVPILPIAAAPGNNDNDNVDDADLDDDDETA 466
>TC206288 homologue to UP|HMGA_SOYBN (Q00423) HMG-Y related protein A (SB16A
protein), complete
Length = 716
Score = 28.5 bits (62), Expect = 4.5
Identities = 17/41 (41%), Positives = 23/41 (55%), Gaps = 2/41 (4%)
Frame = +2
Query: 202 DPSASIDCDEYEATPPTTRPKG--KKAEKRKAKTTDTASST 240
DP+A + +ATP T RP+G KK + A + TA ST
Sbjct: 347 DPNAPPKSPKAKATPATGRPRGRPKKVARSPAVPSPTAVST 469
>TC210087
Length = 670
Score = 28.5 bits (62), Expect = 4.5
Identities = 16/60 (26%), Positives = 25/60 (41%), Gaps = 8/60 (13%)
Frame = +2
Query: 195 GADGTSTDPSASIDCDEYEATPPTTR--------PKGKKAEKRKAKTTDTASSTLSFVPH 246
G + DP +D ++ PP + KGKK +K +K+ + L VPH
Sbjct: 257 GGSYQANDPGVHLDISDFTDQPPESHHHKVEKPGKKGKKKDKEGSKSDHQFAF*LKMVPH 436
>TC217787 weakly similar to UP|Q76EK9 (Q76EK9) Steroid 5alpha-reductase,
partial (77%)
Length = 930
Score = 28.5 bits (62), Expect = 4.5
Identities = 19/41 (46%), Positives = 23/41 (55%), Gaps = 1/41 (2%)
Frame = +2
Query: 203 PSASIDCDEYEATPPTTRPKGKKAEKRKAKTTDTA-SSTLS 242
PS S C + A+ PTT PK + + TT TA SSTLS
Sbjct: 185 PSGSPSCSSHWASTPTT-PKPRPSSPPS*STTSTAPSSTLS 304
>TC205914 weakly similar to UP|NAM8_YEAST (Q00539) NAM8 protein, partial (9%)
Length = 1513
Score = 28.5 bits (62), Expect = 4.5
Identities = 35/147 (23%), Positives = 59/147 (39%), Gaps = 8/147 (5%)
Frame = +3
Query: 148 AEAHALFQVDHKKDFTHENVWRMVKDE-PKWKGQSMKTNSRGQKKSGAGADGTSTDPSAS 206
A H++F D D T + + P +G + T+ + G G S + +
Sbjct: 237 APEHSIFVGDLAPDVTDYLLQETFRAHYPSVRGAKVVTDPNTARSKGYGFVKFSDENERN 416
Query: 207 IDCDEYEATPPTTRPKGKKAEKRKAKTTDTASSTLSFVPHP--DVLAMGKAKMEMMANFR 264
E +TRP A K KTT ++ + VP P V A +++
Sbjct: 417 RAMTEMNGVYCSTRPMRISAATPK-KTTGAYAAPAAPVPKPVYPVPAYTSPVVQVQPPDY 593
Query: 265 EIRNR-----ELDLQQADQQLKQSELQ 286
++ N LDL ++++LKQ+ LQ
Sbjct: 594 DVNNTTIFVGNLDLNVSEEELKQNSLQ 674
>TC206289 UP|HMGB_SOYBN (Q10370) HMG-Y related protein B (SB16B protein)
(Fragment), partial (76%)
Length = 549
Score = 28.5 bits (62), Expect = 4.5
Identities = 17/41 (41%), Positives = 23/41 (55%), Gaps = 2/41 (4%)
Frame = +1
Query: 202 DPSASIDCDEYEATPPTTRPKG--KKAEKRKAKTTDTASST 240
DP+A + +ATP T RP+G KK + A + TA ST
Sbjct: 166 DPNAPPKSPKAKATPATGRPRGRPKKVARSPAVPSPTAVST 288
>TC218189 similar to UP|Q94A38 (Q94A38) AT5g46250/MPL12_3, partial (7%)
Length = 754
Score = 28.5 bits (62), Expect = 4.5
Identities = 24/102 (23%), Positives = 36/102 (34%), Gaps = 4/102 (3%)
Frame = +2
Query: 128 GCYNKVTTPWKSGHSEKDIMAEAHALFQVDHKKDFTHENVWRMVKDEPKWK---GQSMKT 184
G Y WK HSEK+ + +H + HE+ + K GQ +
Sbjct: 77 GKYGHKKQAWKGHHSEKNSSRPEQTGDEENHGSNDHHEDTHEEEDGDHLSKDKGGQRYRN 256
Query: 185 NSRGQKKSGAGADGTSTDPSASIDCDEYEATPPTTR-PKGKK 225
R +K +G + S E +PP R P G +
Sbjct: 257 QGRSRKHKYRAGNGMGHGSAPSTHAAEASKSPPGPRMPDGTR 382
>TC231948 similar to UP|Q6NNI3 (Q6NNI3) At5g28490, partial (69%)
Length = 1023
Score = 28.1 bits (61), Expect = 5.9
Identities = 30/126 (23%), Positives = 41/126 (31%)
Frame = +1
Query: 164 HENVWRMVKDEPKWKGQSMKTNSRGQKKSGAGADGTSTDPSASIDCDEYEATPPTTRPKG 223
H WR W S TNS QK + T PS + A + RP
Sbjct: 535 HRYRWRCAAGRTCWNSCSTLTNSGRQKCT------THHAPSLASPTLRRPAPARSARPGA 696
Query: 224 KKAEKRKAKTTDTASSTLSFVPHPDVLAMGKAKMEMMANFREIRNRELDLQQADQQLKQS 283
A T + P P A + M FR R EL ++ ++
Sbjct: 697 ASTPSSAASAPPTRKTAADPKPTPSAHAPSGSTCMMFVIFRP-RPEELATRRRERGQNNK 873
Query: 284 ELQLRQ 289
LQ++Q
Sbjct: 874 TLQMQQ 891
>TC219720 similar to UP|Q6S7C3 (Q6S7C3) TFIIE-alpha 2 (Fragment), partial
(35%)
Length = 1039
Score = 28.1 bits (61), Expect = 5.9
Identities = 29/126 (23%), Positives = 44/126 (34%), Gaps = 12/126 (9%)
Frame = +3
Query: 92 IRDQFDEYRDFDTPP---------RTGKMLKCRFGKLSKDIQFFTGCYNKVTTPWKSGHS 142
+ DQ +D P R M + G +S D + YN P+
Sbjct: 15 LMDQLSRVKDLPVPEFGSLLAWEARASAMGRAANGDMSGDSKMSQLGYNGAPMPYSG--- 185
Query: 143 EKDIMAEAHALFQVDHKKDFTHENVWRMVKDEPKW---KGQSMKTNSRGQKKSGAGADGT 199
+ ++ + + + KD E +K P W G + RG+ K DGT
Sbjct: 186 DTKVVVDFNGTEDI---KDVKSETGSASLKVLPPWMIKSGMVLTKEQRGEAKEEIKMDGT 356
Query: 200 STDPSA 205
ST SA
Sbjct: 357 STSSSA 374
>TC224292
Length = 670
Score = 28.1 bits (61), Expect = 5.9
Identities = 13/45 (28%), Positives = 27/45 (59%)
Frame = +2
Query: 272 DLQQADQQLKQSELQLRQEELKFKKAENFRAYMDILNKNTSGMND 316
+L +A +QLK+ + + R++E+ ++ F ++ N NT +ND
Sbjct: 101 NLIKAQEQLKKLKTENRKKEMSLLMSQYFTIENNLENSNTVDLND 235
>AW830527 weakly similar to SP|Q91062|VIT_ Vitellogenin precursor (VTG)
[Contains: Lipovitellin LV-1N; Lipovitellin LV-1C;
Lipovitellin LV-2]., partial (2%)
Length = 373
Score = 28.1 bits (61), Expect = 5.9
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -2
Query: 22 VSDTQCEATPDDTQHEGLDDIDLEDEDQS 50
++D Q E DD+Q EG DD + ED+++S
Sbjct: 294 INDNQ-EEGEDDSQDEGEDDSEYEDDEES 211
>CA935575
Length = 410
Score = 28.1 bits (61), Expect = 5.9
Identities = 21/65 (32%), Positives = 32/65 (48%), Gaps = 6/65 (9%)
Frame = +1
Query: 275 QADQQLKQSELQL--RQEELKFKKAENFRAYMDILNKNTSGMN----DEELRTHNALRAF 328
Q + LK+ E ++ QEELK KAE+ + +L+K N +EELR +
Sbjct: 136 QLKENLKEVEAEVIHLQEELKEAKAESMKLKESLLDKENEFQNIFEENEELRLRESTSIK 315
Query: 329 ALSEL 333
+ EL
Sbjct: 316 KVEEL 330
>TC228275 weakly similar to UP|Q9M3D0 (Q9M3D0) Lipase-like protein, partial
(25%)
Length = 1153
Score = 27.7 bits (60), Expect = 7.7
Identities = 20/80 (25%), Positives = 36/80 (45%)
Frame = +2
Query: 66 LVQSWLNISKDPTVGTDQTAAKFWDRIRDQFDEYRDFDTPPRTGKMLKCRFGKLSKDIQF 125
L +W + + VG A + ++++ R + PPR G M + L + Q+
Sbjct: 560 LADTWAAMPDNKMVGK---AIRDPEKLKVIASNPRRYTGPPRVGTMRE-----LLRVTQY 715
Query: 126 FTGCYNKVTTPWKSGHSEKD 145
++KVTTP+ + H D
Sbjct: 716 VQDNFSKVTTPFFTAHGTSD 775
>TC207130 similar to GB|AAH34435.1|21707915|BC034435 ZC3HDC3 protein {Homo
sapiens;} , partial (6%)
Length = 1005
Score = 27.7 bits (60), Expect = 7.7
Identities = 28/96 (29%), Positives = 41/96 (42%), Gaps = 11/96 (11%)
Frame = +1
Query: 193 GAGADGTSTDPSASIDCDEYEATPPTTR--------PKGKKAEKRKAKTTDTASST---L 241
G ADG S C +EAT T+ PK + K++ +T D +S
Sbjct: 406 GYCADGNECRKKHSYVCPTFEATGTCTQGTGCKLHHPKKQSKGKKRKRTADQNNSRGRYF 585
Query: 242 SFVPHPDVLAMGKAKMEMMANFREIRNRELDLQQAD 277
F+P DV G M+A R +N EL+ + +D
Sbjct: 586 GFIP-ADVSKSGM----MVAPKRHRQNGELEEELSD 678
>TC217093
Length = 1829
Score = 27.7 bits (60), Expect = 7.7
Identities = 17/82 (20%), Positives = 34/82 (40%)
Frame = +2
Query: 217 PTTRPKGKKAEKRKAKTTDTASSTLSFVPHPDVLAMGKAKMEMMANFREIRNRELDLQQA 276
P TRPK K + K + + S PH K + + +E+ +
Sbjct: 1208 PITRPKSPKLGRHKGSAVNNSEDKSSSSPHGKQQQNDSTKAKAKGSNKEVIPK------- 1366
Query: 277 DQQLKQSELQLRQEELKFKKAE 298
+ +++S+ +L+ +E +K E
Sbjct: 1367 -KPIRKSQAKLQSQESAIRKTE 1429
>BQ080717
Length = 426
Score = 27.7 bits (60), Expect = 7.7
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = +2
Query: 192 SGAGADGTSTDPSASIDCDEYEATPPTTRPKGKKAEKRKAKTT 234
SG+ TST PS S C + P+T P + K KTT
Sbjct: 257 SGSPTPPTSTSPSPSFRCSKPSCQSPST-PSASCSVKNHTKTT 382
>TC218620 similar to UP|Q8GUA8 (Q8GUA8) Ornithine aminotransferase , partial
(49%)
Length = 960
Score = 27.3 bits (59), Expect = 10.0
Identities = 18/67 (26%), Positives = 29/67 (42%), Gaps = 7/67 (10%)
Frame = +3
Query: 88 FWDRIRDQFDEYR------DFDTP-PRTGKMLKCRFGKLSKDIQFFTGCYNKVTTPWKSG 140
+W +RD +Y + T RTGKML C + ++ DI P +
Sbjct: 15 YWKAVRDICSKYNVSMIADEIQTGLARTGKMLACEWEEVRPDIVILGKALGGGVIPVSAV 194
Query: 141 HSEKDIM 147
++KD+M
Sbjct: 195 LADKDVM 215
>TC229591 UP|O82585 (O82585) NADP-dependent isocitrate dehydrogenase, partial
(97%)
Length = 1668
Score = 27.3 bits (59), Expect = 10.0
Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +3
Query: 228 KRKAKTTDTASSTLSFVPHPDVLAMGKAKMEMMANFREIRN-RELDLQQADQQLKQSELQ 286
+R+ K T L F+P +VL +G+ MEM+ + RN ++L L+Q + + Q L
Sbjct: 927 RREVKPAQTV--LLQFLPGREVLHIGQNWMEMLDCWTSQRNWKQLALEQLNWER*QRILH 1100
Query: 287 LRQEELKF 294
+L+F
Sbjct: 1101FLFMDLRF 1124
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.313 0.129 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,888,829
Number of Sequences: 63676
Number of extensions: 174134
Number of successful extensions: 928
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 854
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 926
length of query: 336
length of database: 12,639,632
effective HSP length: 98
effective length of query: 238
effective length of database: 6,399,384
effective search space: 1523053392
effective search space used: 1523053392
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0223.4