TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0215b.7
         (68 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC232040 weakly similar to UP|Q8LAM9 (Q8LAM9) Pollen coat-like p...   106  2e-24
TC224091 similar to UP|Q8LAM9 (Q8LAM9) Pollen coat-like protein,...    98  9e-22
TC233510 similar to UP|Q8LAM9 (Q8LAM9) Pollen coat-like protein,...    96  3e-21
TC226209 UP|Q9XET0 (Q9XET0) Seed maturation protein PM30, complete     51  1e-07
TC219195 similar to UP|LEA1_CICAR (O49816) Late embryogenesis ab...    43  4e-05
CK605689                                                               35  0.010
TC222193 UP|Q39873 (Q39873) Lea protein precursor, complete            30  0.31
TC225908 similar to UP|Q9ASP7 (Q9ASP7) AT3g46780/T6H20_190, part...    28  0.70
TC225910 similar to UP|Q9ASP7 (Q9ASP7) AT3g46780/T6H20_190, part...    28  0.91
AI855708                                                               28  1.2
TC215251 similar to UP|O81340 (O81340) 26S proteasome regulatory...    27  1.5

>TC232040 weakly similar to UP|Q8LAM9 (Q8LAM9) Pollen coat-like protein,
          partial (85%)
          Length = 326

 Score =  106 bits (265), Expect = 2e-24
 Identities = 53/66 (80%), Positives = 58/66 (87%)
 Frame = +3

Query: 1  MDSQKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAKETVQGAGQQIKASAQGAADAVKNA 60
          MD QKM+Y AGQA+GQA+EKT+NLMDMA NAAQSAKET Q AGQ + ASAQGAADAVKNA
Sbjct: 18 MDPQKMNYQAGQAQGQAQEKTNNLMDMAGNAAQSAKETAQQAGQTVMASAQGAADAVKNA 197

Query: 61 TGMNNN 66
          TGMN N
Sbjct: 198TGMNKN 215


>TC224091 similar to UP|Q8LAM9 (Q8LAM9) Pollen coat-like protein, partial
          (93%)
          Length = 366

 Score = 97.8 bits (242), Expect = 9e-22
 Identities = 48/63 (76%), Positives = 56/63 (88%)
 Frame = +3

Query: 3  SQKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAKETVQGAGQQIKASAQGAADAVKNATG 62
          SQ MSYNAGQAKGQA+EK  N+MD AS+AAQSA+ ++Q AGQQ++A AQGAADAVKNATG
Sbjct: 9  SQNMSYNAGQAKGQAQEKAGNMMDKASSAAQSAQNSMQQAGQQMQAKAQGAADAVKNATG 188

Query: 63 MNN 65
          MNN
Sbjct: 189MNN 197


>TC233510 similar to UP|Q8LAM9 (Q8LAM9) Pollen coat-like protein, partial
          (94%)
          Length = 253

 Score = 96.3 bits (238), Expect = 3e-21
 Identities = 48/64 (75%), Positives = 56/64 (87%)
 Frame = +2

Query: 1  MDSQKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAKETVQGAGQQIKASAQGAADAVKNA 60
          MDSQ MS NAGQAKGQA+EK + +MD ASNAAQSA+E++Q  GQQ++A AQGAADAVKNA
Sbjct: 32 MDSQNMSNNAGQAKGQAQEKANTVMDKASNAAQSAQESLQEVGQQMQAKAQGAADAVKNA 211

Query: 61 TGMN 64
          TGMN
Sbjct: 212TGMN 223


>TC226209 UP|Q9XET0 (Q9XET0) Seed maturation protein PM30, complete
          Length = 727

 Score = 50.8 bits (120), Expect = 1e-07
 Identities = 27/61 (44%), Positives = 32/61 (52%)
 Frame = +3

Query: 1   MDSQKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAKETVQGAGQQIKASAQGAADAVKNA 60
           M S + SY AGQ KG+ EEKT+  M      AQ+AKE  Q   Q  K   Q  A A K+ 
Sbjct: 93  MASHRQSYEAGQTKGRTEEKTNQTMGNIGEKAQAAKEKTQEMAQAAKEKTQQTAQAAKDK 272

Query: 61  T 61
           T
Sbjct: 273 T 275



 Score = 46.2 bits (108), Expect = 3e-06
 Identities = 24/70 (34%), Positives = 37/70 (52%), Gaps = 4/70 (5%)
 Frame = +3

Query: 3   SQKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAKETVQG----AGQQIKASAQGAADAVK 58
           SQ       Q  G A++KTS +      +AQS K+  QG     G+++K +AQGA +AVK
Sbjct: 285 SQAAKEKTQQNTGAAQQKTSEMGQSTKESAQSGKDNTQGFLQQTGEKVKGAAQGATEAVK 464

Query: 59  NATGMNNNSK 68
              G+  + +
Sbjct: 465 QTLGLGEHDQ 494


>TC219195 similar to UP|LEA1_CICAR (O49816) Late embryogenesis abundant
           protein 1 (CapLEA-1), partial (21%)
          Length = 518

 Score = 42.7 bits (99), Expect = 4e-05
 Identities = 27/86 (31%), Positives = 42/86 (48%), Gaps = 22/86 (25%)
 Frame = +2

Query: 1   MDSQKMSYNAGQAKGQAEEKTSNLM-----------DMASNAAQSAKET----------- 38
           M S + ++NAGQ +GQ + K    +           D A+ AA +  +T           
Sbjct: 68  MSSAQQNFNAGQTQGQTQVKAEEFVQSTKETASAATDKANAAANTTGQTAQQNKDESAGF 247

Query: 39  VQGAGQQIKASAQGAADAVKNATGMN 64
           +Q  G+Q+K  AQGA D+VK+  GM+
Sbjct: 248 LQQTGEQVKNMAQGAVDSVKHTLGMD 325


>CK605689 
          Length = 818

 Score = 34.7 bits (78), Expect = 0.010
 Identities = 26/91 (28%), Positives = 40/91 (43%), Gaps = 30/91 (32%)
 Frame = +1

Query: 3   SQKMSYNAGQAKGQAEEKTSNLM--------DMASNAAQSAKETVQGA------------ 42
           +++ +  AG    +A++K  + +        + A +AAQ  KET QGA            
Sbjct: 394 AKEKTSQAGDKAREAKDKAGSYLSETGEAIKNKAQDAAQYTKETAQGAAQYTKETAEAGR 573

Query: 43  ----------GQQIKASAQGAADAVKNATGM 63
                     G+ +K  A GAADAVK+  GM
Sbjct: 574 DKTGGFLSQTGEHVKQMAMGAADAVKHTFGM 666



 Score = 27.3 bits (59), Expect = 1.5
 Identities = 17/58 (29%), Positives = 20/58 (34%)
 Frame = +1

Query: 4   QKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAKETVQGAGQQIKASAQGAADAVKNAT 61
           QK    A  AK +  +        A   AQSAKE      Q  +  A     A K  T
Sbjct: 235 QKAHETAQSAKDKTSQTAQAAQQKAHETAQSAKEKTSQTAQTAQQKAHETTQAAKEKT 408


>TC222193 UP|Q39873 (Q39873) Lea protein precursor, complete
          Length = 1748

 Score = 29.6 bits (65), Expect = 0.31
 Identities = 17/52 (32%), Positives = 22/52 (41%)
 Frame = +3

Query: 8   YNAGQAKGQAEEKTSNLMDMASNAAQSAKETVQGAGQQIKASAQGAADAVKN 59
           Y A + K  A + T         AAQ  K+   G  Q+ K  A GAA   K+
Sbjct: 717 YAAQKTKDYASDATDAAKKTKDYAAQKTKDYASGGAQKTKDYASGAAQKTKD 872


>TC225908 similar to UP|Q9ASP7 (Q9ASP7) AT3g46780/T6H20_190, partial (38%)
          Length = 1293

 Score = 28.5 bits (62), Expect = 0.70
 Identities = 14/53 (26%), Positives = 30/53 (56%)
 Frame = +1

Query: 2   DSQKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAKETVQGAGQQIKASAQGAA 54
           D ++ +Y   Q K +AEE+     + A+ A++SAK+  +   + ++  A+ A+
Sbjct: 520 DGRRKAYAEMQEKAKAEEEARVAAEKANEASESAKKLEEEVKKLLQQEARAAS 678


>TC225910 similar to UP|Q9ASP7 (Q9ASP7) AT3g46780/T6H20_190, partial (32%)
          Length = 1272

 Score = 28.1 bits (61), Expect = 0.91
 Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
 Frame = +2

Query: 2   DSQKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAK---ETVQGAGQQIKASAQGAADAVK 58
           D ++ +Y   Q K +AEE+     + A  A++SAK   E V+   QQ   +A  A +A +
Sbjct: 461 DGRRKAYAEMQEKTKAEEEARVAAEKAREASESAKKLEEEVKRLSQQETRAASLAQEAQE 640

Query: 59  NA 60
            A
Sbjct: 641 KA 646


>AI855708 
          Length = 394

 Score = 27.7 bits (60), Expect = 1.2
 Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
 Frame = +3

Query: 2   DSQKMSYNAGQAKGQAEEKTSNLMDMASNAAQSAK---ETVQGAGQQIKASAQGAADAVK 58
           D ++ +Y   Q K +AEE+     + A  A++SAK   E V+   QQ   +A  A +A +
Sbjct: 132 DGRRKAYAEMQDKTKAEEEARVAAEKAREASESAKKLEEEVKRLSQQETRAASLAQEAQE 311

Query: 59  NA 60
            A
Sbjct: 312 KA 317


>TC215251 similar to UP|O81340 (O81340) 26S proteasome regulatory subunit
           S5A, partial (35%)
          Length = 751

 Score = 27.3 bits (59), Expect = 1.5
 Identities = 14/30 (46%), Positives = 18/30 (59%)
 Frame = +3

Query: 6   MSYNAGQAKGQAEEKTSNLMDMASNAAQSA 35
           M+  AG A  +AE KTS+LMD  +   Q A
Sbjct: 87  MTERAGAATSEAENKTSDLMDDENALLQQA 176


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.295    0.108    0.262 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 970,795
Number of Sequences: 63676
Number of extensions: 3679
Number of successful extensions: 30
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 26
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28
length of query: 68
length of database: 12,639,632
effective HSP length: 44
effective length of query: 24
effective length of database: 9,837,888
effective search space: 236109312
effective search space used: 236109312
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 44 (21.9 bits)
S2: 52 (24.6 bits)