BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206b.6
(90 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC215176 similar to GB|BAC42264.1|26450296|AK117608 GPI-anchored... 31 0.12
TC232888 weakly similar to UP|Q7EC22 (Q7EC22) Gibberellin 20-oxi... 29 0.35
TC234084 similar to UP|Q9FNG9 (Q9FNG9) Gb|AAF02137.1, partial (16%) 28 0.60
CD395017 similar to SP|Q43609|COMT Caffeic acid 3-O-methyltransf... 28 0.78
TC229830 weakly similar to UP|Q9LHF5 (Q9LHF5) Emb|CAB52464.1, pa... 28 1.0
BU761944 similar to PIR|B86411|B864 protein F3M18.4 [imported] -... 28 1.0
CO984031 27 1.7
TC222488 similar to UP|Q8LAR1 (Q8LAR1) S-adenosyl-L-methionine:s... 27 2.3
TC207253 homologue to UP|Q9SDQ5 (Q9SDQ5) Small GTP-binding prote... 27 2.3
TC204619 homologue to UP|Q8W2E3 (Q8W2E3) 3-hydroxy-3-methylgluta... 26 3.0
BG882912 26 3.0
TC234867 26 3.0
TC225389 homologue to UP|Q84T14 (Q84T14) Vacuolar ATPase subunit... 26 3.9
BF596088 25 5.0
TC215197 homologue to PIR|T01588|T01588 GTP-binding protein At2g... 25 5.0
BI967495 25 5.0
TC232263 25 5.0
BM143693 25 5.0
TC229528 25 5.0
TC218580 weakly similar to UP|Q7X8Y6 (Q7X8Y6) Tab2 protein, part... 25 5.0
>TC215176 similar to GB|BAC42264.1|26450296|AK117608 GPI-anchored protein
{Arabidopsis thaliana;} , partial (79%)
Length = 1570
Score = 30.8 bits (68), Expect = 0.12
Identities = 24/80 (30%), Positives = 31/80 (38%)
Frame = +1
Query: 6 FHILMEQFLPLAMWGSLPDEVSAVLNELSSFFRQLCGKVLNVKALDKLQHDIVLTLCHME 65
FH F P A W LP VS S F C +L + +L L + LTL
Sbjct: 58 FHFPTLSFPP*APWIPLPSMVSHTDTSSPSSFFSFCHSILTLHSLITLLPRLKLTLIPSL 237
Query: 66 MLFPLSFFTVSVHLILSLFF 85
F + S+ L+ FF
Sbjct: 238 SRFSIPITPNSLSLLRKRFF 297
>TC232888 weakly similar to UP|Q7EC22 (Q7EC22) Gibberellin 20-oxidase,
partial (8%)
Length = 770
Score = 29.3 bits (64), Expect = 0.35
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -2
Query: 61 LCHMEMLFPLSFFTVSVHLILSLFFLWLLV 90
LCH L +F+ +S HL LS FFL++ +
Sbjct: 133 LCHC*YLMTCAFYKLSNHLYLSHFFLFIKI 44
>TC234084 similar to UP|Q9FNG9 (Q9FNG9) Gb|AAF02137.1, partial (16%)
Length = 418
Score = 28.5 bits (62), Expect = 0.60
Identities = 25/79 (31%), Positives = 31/79 (38%)
Frame = +1
Query: 7 HILMEQFLPLAMWGSLPDEVSAVLNELSSFFRQLCGKVLNVKALDKLQHDIVLTLCHMEM 66
H F P A W SLP VS S F L++ L LQ + LTL
Sbjct: 58 HFPTLSFPP*APWISLPSMVSHTNPSSPSSFSSF---YLSILTLTTLQPQLRLTLTPSSS 228
Query: 67 LFPLSFFTVSVHLILSLFF 85
LF + S +L+ FF
Sbjct: 229 LFSIPITPNSPNLLRKRFF 285
>CD395017 similar to SP|Q43609|COMT Caffeic acid 3-O-methyltransferase (EC
2.1.1.68), partial (35%)
Length = 567
Score = 28.1 bits (61), Expect = 0.78
Identities = 13/44 (29%), Positives = 22/44 (49%)
Frame = -1
Query: 44 VLNVKALDKLQHDIVLTLCHMEMLFPLSFFTVSVHLILSLFFLW 87
VLN ++ L+ VL C++ +F + V +SLFF +
Sbjct: 135 VLNTHVMEFLKKA*VLIFCYLYFIFETNLLATEVSCYISLFFFF 4
>TC229830 weakly similar to UP|Q9LHF5 (Q9LHF5) Emb|CAB52464.1, partial (13%)
Length = 744
Score = 27.7 bits (60), Expect = 1.0
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +1
Query: 47 VKALDKLQHDIVLTLCHMEMLFPLSFFTVSVHLILSLFFL 86
VK +++ D V+ +CH+ LF LSF H ++ FL
Sbjct: 391 VKLGHQIKGDPVIQVCHLLQLFHLSFSVRKSHPLIVFAFL 510
>BU761944 similar to PIR|B86411|B864 protein F3M18.4 [imported] - Arabidopsis
thaliana, partial (10%)
Length = 427
Score = 27.7 bits (60), Expect = 1.0
Identities = 12/26 (46%), Positives = 20/26 (76%), Gaps = 1/26 (3%)
Frame = +3
Query: 58 VLTLCHMEMLFPLSFFTVS-VHLILS 82
++T+C ME + L+FF +S +HL+LS
Sbjct: 246 LMTMCSMERILYLTFFQISALHLLLS 323
>CO984031
Length = 862
Score = 26.9 bits (58), Expect = 1.7
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -1
Query: 57 IVLTLCHMEMLFPLSFFTVSVHLILSLFFLWLL 89
+V+ H+ +L L+FF V + IL LW+L
Sbjct: 106 LVMPSIHIRLLILLAFFCVCLFYILLYSLLWML 8
>TC222488 similar to UP|Q8LAR1 (Q8LAR1) S-adenosyl-L-methionine:salicylic
acid carboxyl methyltransferase-like protein, partial
(17%)
Length = 593
Score = 26.6 bits (57), Expect = 2.3
Identities = 22/72 (30%), Positives = 34/72 (46%), Gaps = 6/72 (8%)
Frame = +1
Query: 23 PDEVSAVLNELSSFFRQLCGKVLNVKALDKLQHDIVL------TLCHMEMLFPLSFFTVS 76
PD+ S V L++ R + G +++ DKL ++ L T E+L L FF
Sbjct: 1 PDDDSEVGRALANSCRSVSGVLVDAHIGDKLSEELFLRVERRATSHGKELLEQLQFF--- 171
Query: 77 VHLILSLFFLWL 88
H++ SL F L
Sbjct: 172-HIVASLSFALL 204
>TC207253 homologue to UP|Q9SDQ5 (Q9SDQ5) Small GTP-binding protein Sar1BNt,
partial (59%)
Length = 817
Score = 26.6 bits (57), Expect = 2.3
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 57 IVLTLCHMEMLFPLSFFTVSVHLILSLFFLWLL 89
++L LC+ + FF VS L+L + WLL
Sbjct: 713 VLLFLCYE*DILVSIFFGVSCQLVLQFYIYWLL 811
>TC204619 homologue to UP|Q8W2E3 (Q8W2E3) 3-hydroxy-3-methylglutaryl coenzyme
A, partial (56%)
Length = 1513
Score = 26.2 bits (56), Expect = 3.0
Identities = 12/39 (30%), Positives = 24/39 (60%)
Frame = -2
Query: 51 DKLQHDIVLTLCHMEMLFPLSFFTVSVHLILSLFFLWLL 89
D L I+++ + +L+P+ + V + L+ L+F+WLL
Sbjct: 1053 DGLLIRILVSNTSLSVLWPVHWTIVDISLLDLLYFMWLL 937
>BG882912
Length = 412
Score = 26.2 bits (56), Expect = 3.0
Identities = 10/28 (35%), Positives = 18/28 (63%)
Frame = +3
Query: 63 HMEMLFPLSFFTVSVHLILSLFFLWLLV 90
H+ + F LSFF +++ +LF +WL +
Sbjct: 192 HLFLSFFLSFFLSIIYVSFALFVVWLAI 275
>TC234867
Length = 309
Score = 26.2 bits (56), Expect = 3.0
Identities = 13/32 (40%), Positives = 22/32 (68%)
Frame = +3
Query: 52 KLQHDIVLTLCHMEMLFPLSFFTVSVHLILSL 83
K + D+ + +CH +L + FF+VSV L++SL
Sbjct: 57 KKKGDMEVPICHSMLL--VHFFSVSVKLLISL 146
>TC225389 homologue to UP|Q84T14 (Q84T14) Vacuolar ATPase subunit E
(Fragment), complete
Length = 1222
Score = 25.8 bits (55), Expect = 3.9
Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = -2
Query: 43 KVLNVKALDKLQ-HDIVLTLCHMEMLFPLSFFTVSVHLILSL 83
+ LN + K+ H I++ + H + SFF + H+ILSL
Sbjct: 471 QTLNNEIFQKVSVHMIIMMMTHSQKFLGCSFFHGTDHVILSL 346
>BF596088
Length = 418
Score = 25.4 bits (54), Expect = 5.0
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 67 LFPLSFFTVSVHLILSLFFLWLL 89
LF S F++ H +LSL LW L
Sbjct: 223 LFIFSIFSLGAHSVLSLSSLWTL 155
>TC215197 homologue to PIR|T01588|T01588 GTP-binding protein At2g44610 -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(97%)
Length = 981
Score = 25.4 bits (54), Expect = 5.0
Identities = 19/57 (33%), Positives = 30/57 (52%)
Frame = +1
Query: 30 LNELSSFFRQLCGKVLNVKALDKLQHDIVLTLCHMEMLFPLSFFTVSVHLILSLFFL 86
L +S F ++LC +L ++ D I L +E+LF T+ HL+LSLF +
Sbjct: 256 LQSVSIFCQKLC--ILKIELFD-CNCGIQLARKDLEVLFQA---TLGTHLLLSLFMM 408
>BI967495
Length = 710
Score = 25.4 bits (54), Expect = 5.0
Identities = 18/47 (38%), Positives = 26/47 (55%), Gaps = 5/47 (10%)
Frame = -1
Query: 49 ALDKLQHDIVL-----TLCHMEMLFPLSFFTVSVHLILSLFFLWLLV 90
A +KL D++L + EML P SF+ V V ++S+ L LLV
Sbjct: 287 AQNKLDPDLILC*LVISRKEKEMLHPFSFYWVLVLGLVSIKLLHLLV 147
>TC232263
Length = 897
Score = 25.4 bits (54), Expect = 5.0
Identities = 24/71 (33%), Positives = 32/71 (44%)
Frame = -1
Query: 20 GSLPDEVSAVLNELSSFFRQLCGKVLNVKALDKLQHDIVLTLCHMEMLFPLSFFTVSVHL 79
G+L S+ + SSFFR LC + L+ L M L S + S L
Sbjct: 600 GTLISSQSSFSSSSSSFFRSLCNPGIIEAKLESLSSSEKTKPRSMNPL--SSSNSRSDKL 427
Query: 80 ILSLFFLWLLV 90
+LSL FL LL+
Sbjct: 426 LLSLLFLVLLL 394
>BM143693
Length = 421
Score = 25.4 bits (54), Expect = 5.0
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 64 MEMLFPLSFFTVSVHLILSLF 84
M + PLS F S+H I+S+F
Sbjct: 317 MTLHLPLSLFKTSIHFIVSVF 379
>TC229528
Length = 918
Score = 25.4 bits (54), Expect = 5.0
Identities = 13/44 (29%), Positives = 22/44 (49%)
Frame = -1
Query: 40 LCGKVLNVKALDKLQHDIVLTLCHMEMLFPLSFFTVSVHLILSL 83
+CG+ + A + IV+ + H+E L L F +H I S+
Sbjct: 351 ICGRSRSHYAAITIIRTIVIAVVHVEPLVTLQFSNSRIHGIFSM 220
>TC218580 weakly similar to UP|Q7X8Y6 (Q7X8Y6) Tab2 protein, partial (7%)
Length = 672
Score = 25.4 bits (54), Expect = 5.0
Identities = 12/32 (37%), Positives = 19/32 (58%)
Frame = +1
Query: 19 WGSLPDEVSAVLNELSSFFRQLCGKVLNVKAL 50
W + SAV E+S+F R +CG L+++ L
Sbjct: 163 WAFVQLPYSAVREEISTFERGVCGSGLDLELL 258
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.333 0.145 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,068,552
Number of Sequences: 63676
Number of extensions: 63296
Number of successful extensions: 907
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 906
length of query: 90
length of database: 12,639,632
effective HSP length: 66
effective length of query: 24
effective length of database: 8,437,016
effective search space: 202488384
effective search space used: 202488384
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0206b.6