BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0201.10
(137 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220681 similar to UP|Q6Z2V7 (Q6Z2V7) NHL repeat-containing pro... 90 4e-19
TC228343 similar to UP|Q6Z2V7 (Q6Z2V7) NHL repeat-containing pro... 87 3e-18
TC228344 77 3e-15
TC220821 similar to UP|Q72D38 (Q72D38) Sodium-dependent symporte... 61 1e-10
TC229294 58 1e-09
AI930730 50 3e-07
CD400517 43 4e-05
TC221000 similar to UP|Q9CSQ9 (Q9CSQ9) Mus musculus 10 days embr... 39 6e-04
CD396933 37 0.002
TC222443 37 0.004
TC232260 36 0.007
TC234016 36 0.007
TC232194 weakly similar to UP|Q9LSN6 (Q9LSN6) Arabidopsis thalia... 35 0.009
TC227116 similar to UP|WAS2_HUMAN (Q9Y6W5) Wiskott-Aldrich syndr... 27 0.051
AW734146 similar to GP|3056592|gb|A T1F9.13 {Arabidopsis thalian... 32 0.12
CD390814 similar to GP|13122434|dbj P0666G04.17 {Oryza sativa (j... 31 0.16
TC225822 homologue to PRF|1609232A.0|226866|1609232A 31kD glycop... 31 0.16
TC224734 homologue to UP|Q09085 (Q09085) Hydroxyproline-rich gly... 30 0.36
TC229883 similar to GB|BAC20813.1|23617133|AP005183 transcriptio... 30 0.36
TC224753 homologue to UP|Q09085 (Q09085) Hydroxyproline-rich gly... 30 0.36
>TC220681 similar to UP|Q6Z2V7 (Q6Z2V7) NHL repeat-containing protein-like,
partial (27%)
Length = 703
Score = 89.7 bits (221), Expect = 4e-19
Identities = 50/115 (43%), Positives = 60/115 (51%), Gaps = 16/115 (13%)
Frame = +3
Query: 18 CSSSSETSLSWWERVRSPENK------EWWWAHGWN---KVREWSEIIVGPKWKTFIRRF 68
C SWWERVRS + + WW+ G K+REWSEI+ GPKWKTFIRRF
Sbjct: 180 CFGGQRRRSSWWERVRSHSHSAPASAGDRWWSRGLRALKKLREWSEIVAGPKWKTFIRRF 359
Query: 69 NRNNNRAGAASYDKKGSFHYDSLSYALNFDDG-------EEDVYSYGGFSTRFAS 116
NRN +Y YD SYALNFD+G D + FSTR+A+
Sbjct: 360 NRNRPNKRIPTY------QYDPFSYALNFDEGPNGDFNHHHDDAALRNFSTRYAA 506
>TC228343 similar to UP|Q6Z2V7 (Q6Z2V7) NHL repeat-containing protein-like,
partial (30%)
Length = 706
Score = 86.7 bits (213), Expect = 3e-18
Identities = 52/136 (38%), Positives = 70/136 (51%), Gaps = 31/136 (22%)
Frame = +1
Query: 14 YLIPCS-----SSSETSLSWWERVRSPENKEW-----------------WWAHG---WNK 48
+ PC S+ WWERVR+ + W WW+ G + K
Sbjct: 163 FCFPCCFGSRRDSATLGFGWWERVRATSS--WSESRSEAQPVTGSPGGRWWSGGVRAFMK 336
Query: 49 VREWSEIIVGPKWKTFIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFDDGEEDVYSYG 108
VREWSE+ GP+WKTFIRRF+R +R+G + + G + YD LSYALNFD+G +
Sbjct: 337 VREWSELAAGPRWKTFIRRFSR--SRSGGSRH-AAGKYQYDPLSYALNFDEGHNGYFDGD 507
Query: 109 G------FSTRFASVP 118
G FSTR+A+ P
Sbjct: 508 GYDGLRNFSTRYAAPP 555
>TC228344
Length = 430
Score = 77.0 bits (188), Expect = 3e-15
Identities = 46/107 (42%), Positives = 61/107 (56%), Gaps = 20/107 (18%)
Frame = +1
Query: 13 CYLIPCSSSSETSLSWWERVR--------SPENKEW---------WWAHG---WNKVREW 52
C+ C S S +WWERVR SP ++ WW+ G + KVREW
Sbjct: 121 CFGSRCHSVS-VGFAWWERVRATSSWSETSPHSEAQPATGSSSGSWWSGGVRAFMKVREW 297
Query: 53 SEIIVGPKWKTFIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFDD 99
SE+ GP+WKTFIRRF+R +R+G A + G + YD LSY+LNFD+
Sbjct: 298 SELAAGPRWKTFIRRFSR--SRSGWARH-AAGKYQYDPLSYSLNFDE 429
>TC220821 similar to UP|Q72D38 (Q72D38) Sodium-dependent symporter family
protein, partial (5%)
Length = 576
Score = 61.2 bits (147), Expect = 1e-10
Identities = 35/87 (40%), Positives = 50/87 (57%), Gaps = 3/87 (3%)
Frame = +2
Query: 41 WWAHGWNKVREWSEIIVGPKWKTFIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFDDG 100
W + K++E+SE+I GPKWKTFIR+ + + +K F YD SYALNF+ G
Sbjct: 218 WLSCKLRKIKEFSEVIAGPKWKTFIRKISGYGRK-----QQQKNRFQYDEHSYALNFNSG 382
Query: 101 E--EDVYSYGGFSTRF-ASVPASTKPT 124
+ ED + FS RF A P++ + T
Sbjct: 383 DKSEDDDTPPSFSARFSAPFPSARRQT 463
>TC229294
Length = 741
Score = 58.2 bits (139), Expect = 1e-09
Identities = 34/80 (42%), Positives = 43/80 (53%), Gaps = 2/80 (2%)
Frame = +1
Query: 39 EWWWAHGWNKVREWSEIIVGPKWKTFIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFD 98
E W K +E SE+I GPKWKTFIR+ + G ++ F YD SYALNF+
Sbjct: 319 ESWVVEKLRKAKEVSEVIAGPKWKTFIRKI----SGYGKKVKQQRNRFQYDEHSYALNFN 486
Query: 99 DG--EEDVYSYGGFSTRFAS 116
G ED FS+RFA+
Sbjct: 487 SGAQSEDEGMPHSFSSRFAA 546
>AI930730
Length = 494
Score = 50.1 bits (118), Expect = 3e-07
Identities = 25/60 (41%), Positives = 34/60 (56%)
Frame = +2
Query: 39 EWWWAHGWNKVREWSEIIVGPKWKTFIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFD 98
E W + K +E SE+I GPKWKTFIR+ + + ++ F YD SYALNF+
Sbjct: 308 ESWVVNKLRKAKEVSEVIAGPKWKTFIRKISGYGKKVKY----QRNRFQYDEHSYALNFN 475
>CD400517
Length = 561
Score = 43.1 bits (100), Expect = 4e-05
Identities = 29/65 (44%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
Frame = -2
Query: 53 SEIIVGPKWKTFIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFDDG--EEDVYSYGGF 110
SEI+V PK +RR + A +K F YD SYALNFDDG EED + F
Sbjct: 344 SEILVWPKKSFSLRRLRTCISEA--KEMKRKMPFQYDPXSYALNFDDGSIEEDDGVFLDF 171
Query: 111 STRFA 115
S R+A
Sbjct: 170 SARYA 156
>TC221000 similar to UP|Q9CSQ9 (Q9CSQ9) Mus musculus 10 days embryo whole
body cDNA, RIKEN full-length enriched library,
clone:2610528D21 product:formin binding protein 4, full
insert sequence. (Fragment), partial (5%)
Length = 727
Score = 39.3 bits (90), Expect = 6e-04
Identities = 27/72 (37%), Positives = 33/72 (45%), Gaps = 5/72 (6%)
Frame = +2
Query: 64 FIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFDD----GEEDVYSYGGFSTRF-ASVP 118
FI R + R +A FHYD+LSYALNF+D E V FS R AS P
Sbjct: 212 FINRIGGRHRRRHSAD------FHYDALSYALNFEDYATADERHVDELKSFSARLPASPP 373
Query: 119 ASTKPTFTPVMV 130
PT + +
Sbjct: 374 PKVSPTSAAIAI 409
>CD396933
Length = 572
Score = 37.4 bits (85), Expect = 0.002
Identities = 21/59 (35%), Positives = 30/59 (50%), Gaps = 5/59 (8%)
Frame = -3
Query: 47 NKVREWSEIIVGPK-----WKTFIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFDDG 100
+K E+S I P+ W+ +RRF R + K SF YD +SY+ NFD+G
Sbjct: 411 HKEYEYSRIYYSPRKRVRRWRNILRRFMRESK---TLCRSKPMSFQYDPVSYSQNFDEG 244
>TC222443
Length = 503
Score = 36.6 bits (83), Expect = 0.004
Identities = 30/103 (29%), Positives = 48/103 (46%)
Frame = +1
Query: 17 PCSSSSETSLSWWERVRSPENKEWWWAHGWNKVREWSEIIVGPKWKTFIRRFNRNNNRAG 76
P S TS SW+ ++ SP + + H +VR G K+ + R ++ ++
Sbjct: 37 PISPQGSTSSSWF-KIMSPTSTNFGDPHP--RVR-------GRSLKSRMGRKLLHHRQSQ 186
Query: 77 AASYDKKGSFHYDSLSYALNFDDGEEDVYSYGGFSTRFASVPA 119
+A F YD SYALNF+D + + FS+R S P+
Sbjct: 187 SAD------FSYDPSSYALNFEDDSPQEFPFRNFSSRLPSSPS 297
>TC232260
Length = 559
Score = 35.8 bits (81), Expect = 0.007
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +2
Query: 82 KKGSFHYDSLSYALNFDDGEEDVYSYGGFSTRFASVPASTKP 123
+ F YD SYALNF+D + + FS+R P S+ P
Sbjct: 146 QSADFSYDPSSYALNFEDDSPEEIPFRNFSSRLPPSPPSSTP 271
>TC234016
Length = 668
Score = 35.8 bits (81), Expect = 0.007
Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Frame = +2
Query: 51 EWSEIIVGPK-----WKTFIRRFNRNNNRAGAASYDKKGSFHYDSLSYALNFDDG 100
E+S I P+ W+ +RRF R + K SF YD +SY+ NFD+G
Sbjct: 164 EYSRIHYNPRKRVRRWRNILRRFMRESK---TLCRSKPMSFQYDPVSYSQNFDEG 319
>TC232194 weakly similar to UP|Q9LSN6 (Q9LSN6) Arabidopsis thaliana genomic
DNA, chromosome 3, TAC clone:K14A17, partial (40%)
Length = 550
Score = 35.4 bits (80), Expect = 0.009
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +1
Query: 20 SSSETSLSWWERVRSPENKEWWWAHGWNKVREW 52
S S T+ W R K WWW WN++R+W
Sbjct: 235 SHSSTTYWIWRRWWKWNWKRWWWLRRWNRLRKW 333
Score = 25.4 bits (54), Expect = 8.9
Identities = 8/25 (32%), Positives = 9/25 (36%)
Frame = +1
Query: 28 WWERVRSPENKEWWWAHGWNKVREW 52
WW WWW W R+W
Sbjct: 337 WWR-------SRWWWTRTWCWDRKW 390
>TC227116 similar to UP|WAS2_HUMAN (Q9Y6W5) Wiskott-Aldrich syndrome protein
family member 2 (WASP-family protein member 2)
(Verprolin homology domain-containing protein 2),
partial (5%)
Length = 1154
Score = 26.9 bits (58), Expect(2) = 0.051
Identities = 12/28 (42%), Positives = 18/28 (63%), Gaps = 1/28 (3%)
Frame = -3
Query: 19 SSSSETSLSWWERV-RSPENKEWWWAHG 45
+S E S+S+WERV S +++W W G
Sbjct: 225 TSKREDSVSFWERVGESMSSRKWVWQCG 142
Score = 24.6 bits (52), Expect(2) = 0.051
Identities = 7/17 (41%), Positives = 10/17 (58%)
Frame = -2
Query: 40 WWWAHGWNKVREWSEII 56
WWW W + R+W E +
Sbjct: 58 WWWRWRWRR-RKWEEAL 11
>AW734146 similar to GP|3056592|gb|A T1F9.13 {Arabidopsis thaliana}, partial
(9%)
Length = 409
Score = 31.6 bits (70), Expect = 0.12
Identities = 17/37 (45%), Positives = 22/37 (58%), Gaps = 2/37 (5%)
Frame = +3
Query: 82 KKGSFHYDSLSYALNFDDG--EEDVYSYGGFSTRFAS 116
++ F YD SYALNF+ G ED FS+RFA+
Sbjct: 264 QRNRFQYDEHSYALNFNSGAQSEDEGMPHSFSSRFAA 374
>CD390814 similar to GP|13122434|dbj P0666G04.17 {Oryza sativa (japonica
cultivar-group)}, partial (14%)
Length = 492
Score = 31.2 bits (69), Expect = 0.16
Identities = 30/95 (31%), Positives = 42/95 (43%), Gaps = 10/95 (10%)
Frame = -1
Query: 32 VRSPENKEW--WWAHGWNKVREWSEIIVGPK---WKTFIRRFNRNNNRAGAASYDKKGSF 86
+RS N W + G++ + +E++ K WK R+ R R + F
Sbjct: 450 IRSNSNLSWSSYERIGYDPIVCVNELVTRLKMGSWKALWRKIKRERRRF----FRPSPVF 283
Query: 87 H--YDSLSYALNFDDG---EEDVYSYGGFSTRFAS 116
H YD SY NFDDG + D S FS RFA+
Sbjct: 282 HVQYDPTSYLQNFDDGYSTDPDNVS-RSFSARFAA 181
>TC225822 homologue to PRF|1609232A.0|226866|1609232A 31kD glycoprotein.
{Glycine max;} , partial (44%)
Length = 820
Score = 31.2 bits (69), Expect = 0.16
Identities = 14/43 (32%), Positives = 22/43 (50%), Gaps = 2/43 (4%)
Frame = +1
Query: 12 ICYLIPCSSSSETSLSW--WERVRSPENKEWWWAHGWNKVREW 52
+C+L CSS ++ W +V SP WWA K+R++
Sbjct: 106 VCFLCCCSSFGSMAMPWRRLPKVPSPNENWLWWAFFGGKMRKF 234
>TC224734 homologue to UP|Q09085 (Q09085) Hydroxyproline-rich glycoprotein
(HRGP) (Fragment), partial (48%)
Length = 990
Score = 30.0 bits (66), Expect = 0.36
Identities = 21/69 (30%), Positives = 25/69 (35%), Gaps = 5/69 (7%)
Frame = -3
Query: 11 CICYLIPCSSSSETSLSWWERVRSPENKEWWWAHGW-----NKVREWSEIIVGPKWKTFI 65
C C+LIP S WW S WWW GW +R W G W+ F+
Sbjct: 559 CCCFLIP----SLLVDGWWRG--SINVNWWWWRLGWWWRAHVVIRRWGRRRWG--WRRFV 404
Query: 66 RRFNRNNNR 74
N R
Sbjct: 403 VVGRMRNGR 377
>TC229883 similar to GB|BAC20813.1|23617133|AP005183 transcription
factor-like protein {Oryza sativa (japonica
cultivar-group);} , partial (15%)
Length = 1063
Score = 30.0 bits (66), Expect = 0.36
Identities = 19/69 (27%), Positives = 29/69 (41%)
Frame = -3
Query: 20 SSSETSLSWWERVRSPENKEWWWAHGWNKVREWSEIIVGPKWKTFIRRFNRNNNRAGAAS 79
S + ++ W R RS +K WW W + +WS I W +I N G S
Sbjct: 461 SGNN*TIVWRGRGRSSRSK*QWWIWRWKWL*QWSTI-----WAMYI---NSRMKWRGRRS 306
Query: 80 YDKKGSFHY 88
+ G +H+
Sbjct: 305 R*RYGGYHH 279
>TC224753 homologue to UP|Q09085 (Q09085) Hydroxyproline-rich glycoprotein
(HRGP) (Fragment), partial (48%)
Length = 1088
Score = 30.0 bits (66), Expect = 0.36
Identities = 21/69 (30%), Positives = 25/69 (35%), Gaps = 5/69 (7%)
Frame = -2
Query: 11 CICYLIPCSSSSETSLSWWERVRSPENKEWWWAHGW-----NKVREWSEIIVGPKWKTFI 65
C C+LIP S WW S WWW GW +R W G W+ F+
Sbjct: 634 CCCFLIP----SLLVDGWWRG--SINVNWWWWRLGWWWRAHVVIRRWGRRRWG--WRRFV 479
Query: 66 RRFNRNNNR 74
N R
Sbjct: 478 VVGRMRNGR 452
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.133 0.452
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,995,929
Number of Sequences: 63676
Number of extensions: 139008
Number of successful extensions: 1466
Number of sequences better than 10.0: 123
Number of HSP's better than 10.0 without gapping: 1404
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1447
length of query: 137
length of database: 12,639,632
effective HSP length: 87
effective length of query: 50
effective length of database: 7,099,820
effective search space: 354991000
effective search space used: 354991000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0201.10