BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0192.12
(60 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC203271 similar to UP|GPX4_CITSI (Q06652) Probable phospholipid... 103 1e-23
TC203397 similar to UP|GPX4_CITSI (Q06652) Probable phospholipid... 103 1e-23
TC203489 similar to UP|GPX4_ARATH (O48646) Probable phospholipid... 97 2e-21
TC203326 similar to UP|GPX4_ARATH (O48646) Probable phospholipid... 97 2e-21
TC226762 similar to UP|GPX1_PEA (O24296) Phospholipid hydroperox... 89 5e-19
TC226619 similar to UP|Q8W259 (Q8W259) Phospholipid hydroperoxid... 89 6e-19
TC216833 similar to GB|AAO39963.1|28372962|BT003735 At2g48150 {A... 88 1e-18
TC214520 similar to UP|Q8W2G9 (Q8W2G9) Glutathione peroxidase, p... 86 3e-18
TC216834 similar to GB|AAO39963.1|28372962|BT003735 At2g48150 {A... 85 7e-18
TC227916 weakly similar to UP|GPX3_ARATH (Q9LYB4) Probable gluta... 79 6e-16
BM886922 weakly similar to GP|28372962|gb At2g48150 {Arabidopsis... 77 1e-15
TC227915 weakly similar to UP|GPX3_ARATH (Q9LYB4) Probable gluta... 77 1e-15
BU761482 similar to GP|28372962|gb| At2g48150 {Arabidopsis thali... 74 1e-14
AW704001 weakly similar to GP|21360380|gb glutathione peroxidase... 72 6e-14
TC203436 similar to UP|Q6QHC9 (Q6QHC9) Phospholipid-hydroperoxid... 65 7e-12
TC226617 similar to UP|Q8W259 (Q8W259) Phospholipid hydroperoxid... 65 9e-12
CD393187 50 4e-11
TC234307 weakly similar to UP|GPX4_MESCR (Q9LEF0) Probable phosp... 61 1e-10
TC214504 similar to UP|GPX4_CITSI (Q06652) Probable phospholipid... 50 1e-08
BE347682 51 1e-07
>TC203271 similar to UP|GPX4_CITSI (Q06652) Probable phospholipid
hydroperoxide glutathione peroxidase (PHGPx)
(Salt-associated protein) , partial (98%)
Length = 778
Score = 103 bits (258), Expect = 1e-23
Identities = 51/60 (85%), Positives = 52/60 (86%)
Frame = +2
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTELS LYEKYK KGLEILAF CNQF AQEPG +EQIQEFV TRFK EFPVFDK
Sbjct: 218 GLTNSNYTELSQLYEKYKQKGLEILAFPCNQFGAQEPGSNEQIQEFVCTRFKAEFPVFDK 397
>TC203397 similar to UP|GPX4_CITSI (Q06652) Probable phospholipid
hydroperoxide glutathione peroxidase (PHGPx)
(Salt-associated protein) , partial (94%)
Length = 1010
Score = 103 bits (258), Expect = 1e-23
Identities = 51/60 (85%), Positives = 52/60 (86%)
Frame = +3
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTELS LYEKYK KGLEILAF CNQF AQEPG +EQIQEFV TRFK EFPVFDK
Sbjct: 450 GLTNSNYTELSQLYEKYKQKGLEILAFPCNQFGAQEPGSNEQIQEFVCTRFKAEFPVFDK 629
>TC203489 similar to UP|GPX4_ARATH (O48646) Probable phospholipid
hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (AtGPX1) , partial (65%)
Length = 606
Score = 96.7 bits (239), Expect = 2e-21
Identities = 46/60 (76%), Positives = 50/60 (82%)
Frame = +1
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTEL+ LYEKYK KGLEILAF CNQF AQEPG +E+I EF TRFK EFP+FDK
Sbjct: 88 GLTNSNYTELNQLYEKYKGKGLEILAFPCNQFGAQEPGTNEEIVEFACTRFKAEFPIFDK 267
>TC203326 similar to UP|GPX4_ARATH (O48646) Probable phospholipid
hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (AtGPX1) , partial (74%)
Length = 749
Score = 96.7 bits (239), Expect = 2e-21
Identities = 46/60 (76%), Positives = 50/60 (82%)
Frame = +2
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTEL+ LYEKYK KGLEILAF CNQF AQEPG +E+I EF TRFK EFP+FDK
Sbjct: 176 GLTNSNYTELNQLYEKYKGKGLEILAFPCNQFGAQEPGTNEEIVEFACTRFKAEFPIFDK 355
>TC226762 similar to UP|GPX1_PEA (O24296) Phospholipid hydroperoxide
glutathione peroxidase, chloroplast precursor (PHGPx) ,
partial (90%)
Length = 1008
Score = 89.0 bits (219), Expect = 5e-19
Identities = 41/60 (68%), Positives = 49/60 (81%)
Frame = +1
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G T+SNY+ELS LYEKYK +GLEILAF CNQF QEPG +E I++F TR+K EFP+FDK
Sbjct: 355 GLTSSNYSELSRLYEKYKNQGLEILAFPCNQFGMQEPGSNEDIKQFACTRYKAEFPIFDK 534
>TC226619 similar to UP|Q8W259 (Q8W259) Phospholipid hydroperoxide
glutathione peroxidase, complete
Length = 956
Score = 88.6 bits (218), Expect = 6e-19
Identities = 41/60 (68%), Positives = 48/60 (79%)
Frame = +3
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G T +NY EL+ LYEKYK +G EILAF CNQF QEPG++E+IQE V TRFK EFP+FDK
Sbjct: 342 GLTQTNYKELNVLYEKYKNQGFEILAFPCNQFAGQEPGNNEEIQEVVCTRFKAEFPIFDK 521
>TC216833 similar to GB|AAO39963.1|28372962|BT003735 At2g48150 {Arabidopsis
thaliana;} , partial (80%)
Length = 538
Score = 87.8 bits (216), Expect = 1e-18
Identities = 39/60 (65%), Positives = 48/60 (80%)
Frame = +2
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GFTNSNYT+L+ LY KYK +GLEILAF CNQF QEPG S++ +EF TR+K E+P+F K
Sbjct: 185 GFTNSNYTQLTELYSKYKDRGLEILAFPCNQFLKQEPGSSQEAEEFACTRYKAEYPIFGK 364
>TC214520 similar to UP|Q8W2G9 (Q8W2G9) Glutathione peroxidase, partial (69%)
Length = 415
Score = 86.3 bits (212), Expect = 3e-18
Identities = 40/60 (66%), Positives = 49/60 (81%)
Frame = +2
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTEL+ LY+KYK +GLEILAF CNQF QEP +++I +FV +RFK EFP+FDK
Sbjct: 161 GLTNSNYTELNQLYDKYKDQGLEILAFPCNQFGKQEPESNDKIVDFVCSRFKSEFPIFDK 340
>TC216834 similar to GB|AAO39963.1|28372962|BT003735 At2g48150 {Arabidopsis
thaliana;} , partial (98%)
Length = 821
Score = 85.1 bits (209), Expect = 7e-18
Identities = 37/60 (61%), Positives = 47/60 (77%)
Frame = +3
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GFTN+NYT+L+ LY KYK +GLEILAF CNQF QEPG S+ ++EF TR+K +P+F K
Sbjct: 243 GFTNTNYTQLTELYSKYKDRGLEILAFPCNQFLKQEPGSSQDVEEFACTRYKAAYPIFGK 422
>TC227916 weakly similar to UP|GPX3_ARATH (Q9LYB4) Probable glutathione
peroxidase At3g63080 , partial (95%)
Length = 1013
Score = 78.6 bits (192), Expect = 6e-16
Identities = 34/60 (56%), Positives = 46/60 (76%)
Frame = +1
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GF ++NYT+L+ LY YK +GLEILAF CNQF +EPG S++ Q+F TR+K E+P+F K
Sbjct: 304 GFADANYTQLTQLYSTYKSRGLEILAFPCNQFLKKEPGTSQEAQDFACTRYKAEYPIFGK 483
>BM886922 weakly similar to GP|28372962|gb At2g48150 {Arabidopsis thaliana},
partial (61%)
Length = 423
Score = 77.4 bits (189), Expect = 1e-15
Identities = 33/60 (55%), Positives = 46/60 (76%)
Frame = +2
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GF ++NY++L+ +Y YK +GLEILAF CNQF +EPG S++ QEF TR+K E+P+F K
Sbjct: 182 GFADANYSQLTQIYSTYKSRGLEILAFPCNQFLKKEPGTSQEAQEFACTRYKAEYPIFGK 361
>TC227915 weakly similar to UP|GPX3_ARATH (Q9LYB4) Probable glutathione
peroxidase At3g63080 , partial (95%)
Length = 885
Score = 77.4 bits (189), Expect = 1e-15
Identities = 33/60 (55%), Positives = 46/60 (76%)
Frame = +1
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GF ++NY++L+ +Y YK +GLEILAF CNQF +EPG S++ QEF TR+K E+P+F K
Sbjct: 235 GFADANYSQLTQIYSTYKSRGLEILAFPCNQFLKKEPGTSQEAQEFACTRYKAEYPIFGK 414
>BU761482 similar to GP|28372962|gb| At2g48150 {Arabidopsis thaliana},
partial (54%)
Length = 445
Score = 74.3 bits (181), Expect = 1e-14
Identities = 34/50 (68%), Positives = 40/50 (80%)
Frame = +3
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TR 50
GFTNSNYT+L+ LY KYK +GLEILAF CNQF QEPG S++ +EF TR
Sbjct: 294 GFTNSNYTQLTELYSKYKDRGLEILAFPCNQFLKQEPGSSQEAEEFACTR 443
>AW704001 weakly similar to GP|21360380|gb glutathione peroxidase 1 {Oryza
sativa}, partial (80%)
Length = 424
Score = 72.0 bits (175), Expect = 6e-14
Identities = 40/60 (66%), Positives = 42/60 (69%)
Frame = +2
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G TNSNYTELS EKYK KGLE LAF NQ AQEPG +EQ+Q V TRFK E P DK
Sbjct: 122 GLTNSNYTELSQRCEKYKQKGLEKLAFPYNQSGAQEPGSNEQMQ*SVCTRFKAE*PELDK 301
>TC203436 similar to UP|Q6QHC9 (Q6QHC9) Phospholipid-hydroperoxide
glutathione peroxidase , partial (97%)
Length = 1109
Score = 65.1 bits (157), Expect = 7e-12
Identities = 30/40 (75%), Positives = 33/40 (82%)
Frame = +2
Query: 21 GLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
GLEILAF CNQF AQEPG +E+I EF TRFK EFP+FDK
Sbjct: 497 GLEILAFPCNQFGAQEPGTNEEIVEFACTRFKAEFPIFDK 616
Score = 39.3 bits (90), Expect = 4e-04
Identities = 17/22 (77%), Positives = 19/22 (86%)
Frame = +3
Query: 1 GFTNSNYTELSPLYEKYK*KGL 22
G TNSNYTEL+ LYEKYK KG+
Sbjct: 252 GLTNSNYTELNQLYEKYKGKGM 317
>TC226617 similar to UP|Q8W259 (Q8W259) Phospholipid hydroperoxide
glutathione peroxidase, partial (59%)
Length = 871
Score = 64.7 bits (156), Expect = 9e-12
Identities = 29/40 (72%), Positives = 33/40 (82%)
Frame = +3
Query: 21 GLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G EILAF CNQF QEPG++E+IQE V TRFK EFP+FDK
Sbjct: 78 GFEILAFPCNQFAGQEPGNNEEIQEVVCTRFKAEFPIFDK 197
>CD393187
Length = 566
Score = 49.7 bits (117), Expect(2) = 4e-11
Identities = 23/39 (58%), Positives = 29/39 (73%)
Frame = -2
Query: 22 LEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
LEILAF CNQF QEP +++I +FV + FK EFP+F K
Sbjct: 418 LEILAFPCNQFGKQEPESNDKIVDFVCSGFKSEFPIFHK 302
Score = 32.7 bits (73), Expect(2) = 4e-11
Identities = 13/17 (76%), Positives = 15/17 (87%)
Frame = -3
Query: 1 GFTNSNYTELSPLYEKY 17
G TNSNYTEL+ LY+KY
Sbjct: 492 GLTNSNYTELNQLYDKY 442
>TC234307 weakly similar to UP|GPX4_MESCR (Q9LEF0) Probable phospholipid
hydroperoxide glutathione peroxidase (PHGPx) , partial
(66%)
Length = 558
Score = 60.8 bits (146), Expect = 1e-10
Identities = 29/60 (48%), Positives = 41/60 (68%)
Frame = +2
Query: 1 GFTNSNYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
G T +NY +L+ +Y +YK +GLEI F CNQF QEPG S I+EF+ + K+ + V+DK
Sbjct: 149 GLTRANYAQLNDIYNEYKDRGLEIAGFPCNQFGNQEPGCSVDIKEFL-LKNKVTWDVYDK 325
>TC214504 similar to UP|GPX4_CITSI (Q06652) Probable phospholipid
hydroperoxide glutathione peroxidase (PHGPx)
(Salt-associated protein) , partial (84%)
Length = 857
Score = 49.7 bits (117), Expect(2) = 1e-08
Identities = 23/39 (58%), Positives = 29/39 (73%)
Frame = +3
Query: 22 LEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFDK 60
LEILAF CNQF QEP +++I +FV + FK EFP+F K
Sbjct: 408 LEILAFPCNQFGKQEPESNDKIVDFVCSGFKSEFPIFHK 524
Score = 24.6 bits (52), Expect(2) = 1e-08
Identities = 10/13 (76%), Positives = 11/13 (83%)
Frame = +2
Query: 1 GFTNSNYTELSPL 13
G TNSNYTEL+ L
Sbjct: 368 GLTNSNYTELNQL 406
>BE347682
Length = 163
Score = 50.8 bits (120), Expect = 1e-07
Identities = 27/54 (50%), Positives = 36/54 (66%)
Frame = -1
Query: 6 NYTELSPLYEKYK*KGLEILAFSCNQFRAQEPGDSEQIQEFV*TRFKIEFPVFD 59
+YT+LS LY++ K K L ILAF Q+ A E +E+ +E TRFK EFP+FD
Sbjct: 163 SYTKLSQLYKESKYKDLIILAFPYKQYGALESVYNEETEELASTRFKDEFPIFD 2
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.337 0.151 0.469
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,154,901
Number of Sequences: 63676
Number of extensions: 16527
Number of successful extensions: 98
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of query: 60
length of database: 12,639,632
effective HSP length: 36
effective length of query: 24
effective length of database: 10,347,296
effective search space: 248335104
effective search space used: 248335104
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0192.12