BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0185.2
(122 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228097 100 9e-23
TC219449 92 3e-20
CO982038 92 3e-20
TC203476 weakly similar to PIR|C86287|C86287 F9L1.24 protein - A... 92 4e-20
CO978969 91 6e-20
TC203507 91 6e-20
BI969375 67 1e-12
TC213060 64 1e-11
AW102128 38 7e-04
TC232340 similar to GB|AAP37726.1|30725408|BT008367 At4g04670 {A... 28 0.59
TC218664 weakly similar to GB|AAP37831.1|30725618|BT008472 At4g3... 28 0.77
AW568877 28 0.77
TC229279 homologue to UP|Q9XH50 (Q9XH50) 1-D-deoxyxylulose 5-pho... 27 1.7
TC217779 similar to GB|AAO11592.1|27363346|BT002676 At4g31560/F3... 27 1.7
BI971272 26 2.2
BU544848 26 2.9
AI461176 similar to GP|10177317|dbj cleavage stimulation factor ... 26 2.9
TC220713 similar to UP|Q94KA1 (Q94KA1) F6N15.8-like protein (Fra... 26 2.9
BM309730 similar to PIR|T01364|T01 homeodomain transcription fac... 26 2.9
TC224612 homologue to UP|Q9ZSR1 (Q9ZSR1) Cytoplasmic glucose-6-p... 25 3.8
>TC228097
Length = 832
Score = 100 bits (249), Expect = 9e-23
Identities = 48/87 (55%), Positives = 65/87 (74%), Gaps = 1/87 (1%)
Frame = +2
Query: 4 NELTKYLEDGLEERGS-LDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGR 62
++L KYLE+ + R S +ILNWWK++ RYPIL+ +AR++L P+ST+A E FS GGR
Sbjct: 323 SDLDKYLEEPIFPRNSDFNILNWWKVHMPRYPILSMMARDVLGTPMSTMAPELAFSTGGR 502
Query: 63 VVDPYRSSLTPKTLEALICTQDWIKGK 89
V+D RSSL P T EALICTQDW++ +
Sbjct: 503 VLDSSRSSLNPDTREALICTQDWLQNE 583
>TC219449
Length = 702
Score = 92.0 bits (227), Expect = 3e-20
Identities = 43/90 (47%), Positives = 64/90 (70%), Gaps = 1/90 (1%)
Frame = +3
Query: 1 GSKNELTKYLEDGLEERG-SLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSA 59
G+K++L KYLE+ L R +ILNWW+++ RYP+L+ +AR +L IP+S VA E F+
Sbjct: 192 GTKSDLDKYLEEPLFPRNVDFNILNWWRVHTPRYPVLSMMARNVLGIPMSKVAPELAFNH 371
Query: 60 GGRVVDPYRSSLTPKTLEALICTQDWIKGK 89
RV+D SSL P T++AL+C+QDWI+ +
Sbjct: 372 SARVLDRDWSSLNPATVQALVCSQDWIRSE 461
>CO982038
Length = 675
Score = 92.0 bits (227), Expect = 3e-20
Identities = 46/85 (54%), Positives = 59/85 (69%), Gaps = 1/85 (1%)
Frame = -1
Query: 3 KNELTKYL-EDGLEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGG 61
K+EL YL ED L DIL WWK N +YP L +IA+++LAI +STVASES FS GG
Sbjct: 366 KSELDNYL*EDVLPRAVDFDILMWWKFNGVKYPTLQAIAKDILAILVSTVASESAFSTGG 187
Query: 62 RVVDPYRSSLTPKTLEALICTQDWI 86
+V+ P+RS L TLEAL+C + W+
Sbjct: 186 QVLSPHRSRLQWTTLEALMCARSWL 112
>TC203476 weakly similar to PIR|C86287|C86287 F9L1.24 protein - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (19%)
Length = 1227
Score = 91.7 bits (226), Expect = 4e-20
Identities = 40/86 (46%), Positives = 62/86 (71%), Gaps = 1/86 (1%)
Frame = +1
Query: 3 KNELTKYLEDGLEER-GSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGG 61
K+EL +YLE+ L R D+L WWKLN +YP L+ +AR++L++P+S+V ES F
Sbjct: 667 KSELDQYLEESLLPRVPDFDVLGWWKLNKLKYPTLSKMARDILSVPVSSVPPESVFDTKV 846
Query: 62 RVVDPYRSSLTPKTLEALICTQDWIK 87
+ +D YRSSL P+T+EA++C +DW++
Sbjct: 847 KEMDQYRSSLRPETVEAIVCAKDWMQ 924
>CO978969
Length = 747
Score = 91.3 bits (225), Expect = 6e-20
Identities = 43/88 (48%), Positives = 63/88 (70%), Gaps = 1/88 (1%)
Frame = -3
Query: 3 KNELTKYLEDGLEER-GSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGG 61
K EL +YLE+ LE R DIL+WW++N +YP L+ +A ++L +PIST+++++ F
Sbjct: 361 KTELDEYLEEPLEPRVQEFDILSWWRINGLKYPTLSRLASDILCMPISTLSADAVFDTEI 182
Query: 62 RVVDPYRSSLTPKTLEALICTQDWIKGK 89
R +D YRSSL TLEALICT+DW++ K
Sbjct: 181 RKMDSYRSSLDSLTLEALICTKDWLQYK 98
>TC203507
Length = 709
Score = 91.3 bits (225), Expect = 6e-20
Identities = 40/86 (46%), Positives = 62/86 (71%), Gaps = 1/86 (1%)
Frame = +3
Query: 3 KNELTKYLEDGLEER-GSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGG 61
K+EL +YLE+ L R D+L WWKLN +YP L+ +AR++L++P+S++ ES F
Sbjct: 120 KSELDQYLEESLLPRVPDFDVLGWWKLNKLKYPTLSKMARDILSVPVSSLPPESVFDTKV 299
Query: 62 RVVDPYRSSLTPKTLEALICTQDWIK 87
+ +D YRSSL P+T+EAL+C +DW++
Sbjct: 300 KEMDQYRSSLRPETVEALVCAKDWMQ 377
>BI969375
Length = 510
Score = 66.6 bits (161), Expect = 1e-12
Identities = 32/86 (37%), Positives = 54/86 (62%), Gaps = 1/86 (1%)
Frame = -3
Query: 2 SKNELTKYLEDGLEER-GSLDILNWWKLNASRYPILASIARELLAIPISTVASESTFSAG 60
+K E+ + LE+ L R D+L WW++N + L+ + R++L+ P+ T A +S +
Sbjct: 376 TKYEIDQCLEESLLPRVPHSDVLAWWEINTIKCLTLSKMGRDILSTPVFTGALDSVCYSK 197
Query: 61 GRVVDPYRSSLTPKTLEALICTQDWI 86
+ +D YRSSL P+TLEAL+C + W+
Sbjct: 196 SKEIDEYRSSLRPETLEALVCAKGWM 119
>TC213060
Length = 499
Score = 63.5 bits (153), Expect = 1e-11
Identities = 29/87 (33%), Positives = 51/87 (58%), Gaps = 4/87 (4%)
Frame = +2
Query: 21 DILNWWKLNASRYPILASIARELLAIPISTVASESTFSAGGRVVDPYRSSLTPKTLEALI 80
D+L WWK+N++RYP L+ +AR+ LA+ ++V E F G +D R + + +A++
Sbjct: 131 DVLEWWKVNSTRYPRLSVMARDFLAVQATSVVPEELFCGKGDEIDKQRICMPHDSTQAIL 310
Query: 81 CTQDWI----KGKYSKSLLSNEEVMEL 103
C + WI K K+ + + E +M+L
Sbjct: 311 CIKSWIQVGVKFKFKSTEIDYERLMKL 391
>AW102128
Length = 426
Score = 37.7 bits (86), Expect = 7e-04
Identities = 16/50 (32%), Positives = 32/50 (64%)
Frame = +2
Query: 38 SIARELLAIPISTVASESTFSAGGRVVDPYRSSLTPKTLEALICTQDWIK 87
++AR++L++P++++ ES +D Y SL P+T +AL+C D ++
Sbjct: 8 TMARDILSVPVTSLPPESVCDTEVT*MDQYLRSLRPQTEQALVCATDCMR 157
>TC232340 similar to GB|AAP37726.1|30725408|BT008367 At4g04670 {Arabidopsis
thaliana;} , partial (13%)
Length = 844
Score = 28.1 bits (61), Expect = 0.59
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 37 ASIARELLAIPISTVASESTFSAGGRVVDP 66
A I ELL++P++ V +ES F + +V P
Sbjct: 479 AKIRTELLSVPVAKVNAESGFRSSENIVQP 568
>TC218664 weakly similar to GB|AAP37831.1|30725618|BT008472 At4g31350
{Arabidopsis thaliana;} , partial (29%)
Length = 673
Score = 27.7 bits (60), Expect = 0.77
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = -2
Query: 14 LEERGSLDILNWWKLNASRYPILASIARELLAIPISTVASES 55
L++ L +LN W ++A+ YP+ +E ++ I +ASES
Sbjct: 360 LDQNSRLKVLNNWPIDATNYPLF----QE*IS*QIGNMASES 247
>AW568877
Length = 362
Score = 27.7 bits (60), Expect = 0.77
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = +3
Query: 60 GGRVVDPYRSSLTPKTLEALI--CTQDWIKGKYSKSLLSNEEVMELEKYEQGIITNY 114
G R+ + + TP+ +A + D + K KSL SNE + +L+KY+ I +Y
Sbjct: 132 GRRLYSSFSNDSTPELSDAYSHKSSSDSEENKKGKSLSSNEILKKLKKYKISKILSY 302
>TC229279 homologue to UP|Q9XH50 (Q9XH50) 1-D-deoxyxylulose 5-phosphate
synthase, partial (15%)
Length = 1074
Score = 26.6 bits (57), Expect = 1.7
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = -2
Query: 4 NELTKYLEDGLEERGSLDILNWWKLNASR 32
NE+TK+ + E DILN+ KL A R
Sbjct: 788 NEVTKFYKSYTERMRKFDILNYQKLFAMR 702
>TC217779 similar to GB|AAO11592.1|27363346|BT002676 At4g31560/F3L17_130
{Arabidopsis thaliana;} , partial (22%)
Length = 722
Score = 26.6 bits (57), Expect = 1.7
Identities = 21/67 (31%), Positives = 32/67 (47%)
Frame = +2
Query: 41 RELLAIPISTVASESTFSAGGRVVDPYRSSLTPKTLEALICTQDWIKGKYSKSLLSNEEV 100
RE ++T A ST S + P SSL T+ A + D + + + + NE +
Sbjct: 137 RERRGSAVATRAGPSTSSILFAIALP--SSLLGVTILAALRMGDKLDRDWLEEMARNEAL 310
Query: 101 MELEKYE 107
MELE Y+
Sbjct: 311 MELETYD 331
>BI971272
Length = 782
Score = 26.2 bits (56), Expect = 2.2
Identities = 12/43 (27%), Positives = 22/43 (50%)
Frame = +3
Query: 73 PKTLEALICTQDWIKGKYSKSLLSNEEVMELEKYEQGIITNYV 115
P+ +E+ + WI+ S +LLS + L K + ++ YV
Sbjct: 42 PRYMESNCVSSQWIQRNISNALLSTYSIFSLAKTQLLLVEIYV 170
>BU544848
Length = 542
Score = 25.8 bits (55), Expect = 2.9
Identities = 14/38 (36%), Positives = 18/38 (46%)
Frame = +2
Query: 48 ISTVASESTFSAGGRVVDPYRSSLTPKTLEALICTQDW 85
+ T+ FS GG+VVD Y S T K +C W
Sbjct: 392 VRTMDFNREFSKGGKVVDIYVSRRTNKGGFVWVCVV*W 505
>AI461176 similar to GP|10177317|dbj cleavage stimulation factor subunit
1-like protein {Arabidopsis thaliana}, partial (18%)
Length = 409
Score = 25.8 bits (55), Expect = 2.9
Identities = 16/42 (38%), Positives = 21/42 (49%)
Frame = -1
Query: 41 RELLAIPISTVASESTFSAGGRVVDPYRSSLTPKTLEALICT 82
R L ++P TV SE S + PKTLEA+IC+
Sbjct: 127 RFL*SLPSCTVCSELLLSISEERERETANWTEPKTLEAVICS 2
>TC220713 similar to UP|Q94KA1 (Q94KA1) F6N15.8-like protein (Fragment),
partial (45%)
Length = 665
Score = 25.8 bits (55), Expect = 2.9
Identities = 20/62 (32%), Positives = 28/62 (44%), Gaps = 6/62 (9%)
Frame = -1
Query: 23 LNWWKLNASRYPILASIARELLAIPISTVASESTFS------AGGRVVDPYRSSLTPKTL 76
+N+ N+SRY + A A E +A + S STF V +P P+TL
Sbjct: 521 INY*SKNSSRYRV*AVAATEGIASFQLILVSHSTFQ*YHAALKSCEVTEPTMKRPNPRTL 342
Query: 77 EA 78
EA
Sbjct: 341 EA 336
>BM309730 similar to PIR|T01364|T01 homeodomain transcription factor
(ATHB-14) [imported] - Arabidopsis thaliana, partial
(16%)
Length = 428
Score = 25.8 bits (55), Expect = 2.9
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = +2
Query: 20 LDILNWWKLNASRYPILAS 38
+DILNWW AS Y + S
Sbjct: 359 IDILNWWPHRASFYNLCTS 415
>TC224612 homologue to UP|Q9ZSR1 (Q9ZSR1) Cytoplasmic glucose-6-phosphate
1-dehydrogenase , partial (38%)
Length = 791
Score = 25.4 bits (54), Expect = 3.8
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +2
Query: 62 RVVDPYRSSLTPKTLEALICTQDWIKGK 89
++ PY SLTP+ L+ L C GK
Sbjct: 29 KMTQPYLMSLTPQHLQLLFCEYTMKDGK 112
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.139 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,230,402
Number of Sequences: 63676
Number of extensions: 60043
Number of successful extensions: 327
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 327
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 327
length of query: 122
length of database: 12,639,632
effective HSP length: 98
effective length of query: 24
effective length of database: 6,399,384
effective search space: 153585216
effective search space used: 153585216
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0185.2