BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0180a.12
(1023 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228023 similar to UP|Q9FLH2 (Q9FLH2) 2-oxoglutarate dehydrogen... 715 0.0
TC220075 similar to UP|O49541 (O49541) Oxoglutarate dehydrogenas... 354 1e-97
TC209261 similar to UP|Q9FLH2 (Q9FLH2) 2-oxoglutarate dehydrogen... 201 3e-90
BG726584 279 5e-75
BU551159 weakly similar to GP|28416717|gb At3g55410 {Arabidopsis... 152 7e-37
TC228024 weakly similar to UP|O49541 (O49541) Oxoglutarate dehyd... 111 2e-24
CA800991 similar to GP|10177333|db 2-oxoglutarate dehydrogenase ... 53 6e-07
TC206696 similar to UP|O24457 (O24457) Pyruvate dehydrogenase E1... 39 0.009
TC206697 similar to UP|O24457 (O24457) Pyruvate dehydrogenase E1... 36 0.075
TC217476 similar to UP|O63201 (O63201) Cytochrome oxidase I (Fra... 31 2.4
TC223633 weakly similar to UP|Q93VQ6 (Q93VQ6) At1g07080/F10K1_15... 30 4.1
TC218033 similar to UP|Q9FFJ5 (Q9FFJ5) Gb|AAD32776.1 (At5g05830)... 30 5.4
TC228841 29 9.2
BF595497 29 9.2
>TC228023 similar to UP|Q9FLH2 (Q9FLH2) 2-oxoglutarate dehydrogenase, E1
component, partial (36%)
Length = 1591
Score = 715 bits (1845), Expect = 0.0
Identities = 337/378 (89%), Positives = 365/378 (96%)
Frame = +2
Query: 646 LAFATLIVEGNHVRLSGQDVERGTFSHRHAVVHDQATGEKYCPLDHVIMNQNEEMFTVSN 705
LA+ATL++EGNHVRLSGQDVERGTFSHRHAVVHDQ TGEKYCPLD+VIMNQ+EEMFTVSN
Sbjct: 8 LAYATLLIEGNHVRLSGQDVERGTFSHRHAVVHDQTTGEKYCPLDNVIMNQDEEMFTVSN 187
Query: 706 SSLSEFGVLGFELGYSMENPNSLVIWEAQFGDFANGAQVIFDNFLSSGESKWLRQTGLVV 765
SSLSEFGVLGFELGYSMENPNSL+IWEAQFGDFANGA VIFDNFL+SGE+KWLRQTGLVV
Sbjct: 188 SSLSEFGVLGFELGYSMENPNSLIIWEAQFGDFANGAHVIFDNFLASGEAKWLRQTGLVV 367
Query: 766 LLPHGYDGQGPEHSSGRLERYLQMADDHPYIIPEMDPTLRKQIQECNLQIVNVTTPANFF 825
LLPHGYDGQGPEHSS RLER+LQMADD+P++IPEMDPTLRKQIQECNLQIVNVTTPANFF
Sbjct: 368 LLPHGYDGQGPEHSSARLERFLQMADDNPHVIPEMDPTLRKQIQECNLQIVNVTTPANFF 547
Query: 826 HVLRRQIHREFRKPLIVMSPKNLLRSKVCRSNLSEFDDVQGHPGFDKQGTRFKRLIKDQN 885
HVLRRQ+HR+FRKPLIVMSPKNLLRSK CRSNLSEFDDVQGHPGFDKQGTRFKRLIKDQN
Sbjct: 548 HVLRRQVHRDFRKPLIVMSPKNLLRSKACRSNLSEFDDVQGHPGFDKQGTRFKRLIKDQN 727
Query: 886 DHSNVEEGIRRLILCSGKVYYELDEQRTKDDAKDVAICRVEQLCPFPYDLVQRELKRYPN 945
H +VEEGIRRL+LCSGKVYYELDEQRTK DA DVAICRVEQLCPFPYDLVQRELKRYPN
Sbjct: 728 AHKDVEEGIRRLVLCSGKVYYELDEQRTKVDANDVAICRVEQLCPFPYDLVQRELKRYPN 907
Query: 946 AEVVWCQEEPMNMGGYSYILPRLITSMKALGRGGYEDVKYVGRAPSAATATGFLKVHQRE 1005
AEVVWCQEEPMNMGGY+Y+LPRLI+SMKA+ RGGY+DVKY+GRAPSAATATGFLKVHQ+E
Sbjct: 908 AEVVWCQEEPMNMGGYTYVLPRLISSMKAVNRGGYDDVKYIGRAPSAATATGFLKVHQKE 1087
Query: 1006 QAELVHKAMQHKPINFPY 1023
Q E+ KA+Q +PI+FP+
Sbjct: 1088 QTEIAEKAVQQEPIDFPF 1141
>TC220075 similar to UP|O49541 (O49541) Oxoglutarate dehydrogenase - like
protein , partial (19%)
Length = 566
Score = 354 bits (908), Expect = 1e-97
Identities = 166/188 (88%), Positives = 180/188 (95%)
Frame = +2
Query: 463 IFHVNGDDVESVVHVCELAAEWRQTFHSDVVVDLVCYRRFGHNEIDEPSFTQPKMYKVIR 522
IFHVNGDDVE+VVH CELAAEWRQTFHSDVVVDLVCYRRFGHNEIDEPSFTQPKMYKVIR
Sbjct: 2 IFHVNGDDVEAVVHACELAAEWRQTFHSDVVVDLVCYRRFGHNEIDEPSFTQPKMYKVIR 181
Query: 523 NHPSALEIYQKKLLELGELTQEDIDKIHKKVTSILNDEFLASKDYIPKRRDWLSAYWSGF 582
+HPS LEIY+K LLE GELTQE+ID+IHKKVTSILN+EFLASK+YIPKRRDWLSAYW GF
Sbjct: 182 SHPSTLEIYEKNLLESGELTQEEIDRIHKKVTSILNEEFLASKEYIPKRRDWLSAYWLGF 361
Query: 583 KSPEQLSRIRNTGVKPDILKNVGKAITALPESLNPHRAVKKVYEQRAQMVETGEDIDWGF 642
KSPEQLSRIRNTGVKP+ILKNVGKAIT +PE+ PHRAVK++YEQRAQM+ETGEDIDWGF
Sbjct: 362 KSPEQLSRIRNTGVKPEILKNVGKAITTIPENFTPHRAVKRIYEQRAQMIETGEDIDWGF 541
Query: 643 AEALAFAT 650
AEALA+AT
Sbjct: 542 AEALAYAT 565
>TC209261 similar to UP|Q9FLH2 (Q9FLH2) 2-oxoglutarate dehydrogenase, E1
component, partial (18%)
Length = 766
Score = 201 bits (510), Expect(2) = 3e-90
Identities = 96/111 (86%), Positives = 101/111 (90%)
Frame = +2
Query: 831 QIHREFRKPLIVMSPKNLLRSKVCRSNLSEFDDVQGHPGFDKQGTRFKRLIKDQNDHSNV 890
QIHREFRKPLIVM KNLLRSK CRSNLSEFDDVQGHPGFDKQGT FKRLIKDQN HS+V
Sbjct: 218 QIHREFRKPLIVMPAKNLLRSKTCRSNLSEFDDVQGHPGFDKQGT*FKRLIKDQNKHSDV 397
Query: 891 EEGIRRLILCSGKVYYELDEQRTKDDAKDVAICRVEQLCPFPYDLVQRELK 941
EEGIRRL+LCSGKVYYELDEQRTK+DAKDVA+CRVE+LCPFPYD R K
Sbjct: 398 EEGIRRLVLCSGKVYYELDEQRTKEDAKDVAVCRVEKLCPFPYDCPTRAQK 550
Score = 151 bits (381), Expect(2) = 3e-90
Identities = 72/79 (91%), Positives = 75/79 (94%)
Frame = +3
Query: 935 LVQRELKRYPNAEVVWCQEEPMNMGGYSYILPRLITSMKALGRGGYEDVKYVGRAPSAAT 994
+VQRELKRYPNAEVVWCQEEPMNMGGY+YILPRLITSMKA+GRGGYEDVKY GRAPSAAT
Sbjct: 528 IVQRELKRYPNAEVVWCQEEPMNMGGYTYILPRLITSMKAVGRGGYEDVKYAGRAPSAAT 707
Query: 995 ATGFLKVHQREQAELVHKA 1013
ATGFLKVH EQAELV KA
Sbjct: 708 ATGFLKVHLNEQAELVQKA 764
>BG726584
Length = 419
Score = 279 bits (713), Expect = 5e-75
Identities = 133/139 (95%), Positives = 136/139 (97%)
Frame = +3
Query: 367 HLEAVNPLVVGKTRAKQYYSNDAERMKNMGVLIHGDGSFAGQGVVYETLHLSALPNYTTG 426
HLEAVNPLVVGKTRAKQYYSND +RMKNMGVLIHGDGSFAGQGVVYETLHLSALPNYTTG
Sbjct: 3 HLEAVNPLVVGKTRAKQYYSNDVDRMKNMGVLIHGDGSFAGQGVVYETLHLSALPNYTTG 182
Query: 427 GTIHIVFNNQVAFTTDPESGRSSQYSTDVAKALNAPIFHVNGDDVESVVHVCELAAEWRQ 486
GTIHIVFNNQVAFTTDP+SGRSSQY TDVAKALNAPIFHVNGDDVE+VVHVCELAAEWRQ
Sbjct: 183 GTIHIVFNNQVAFTTDPKSGRSSQYCTDVAKALNAPIFHVNGDDVEAVVHVCELAAEWRQ 362
Query: 487 TFHSDVVVDLVCYRRFGHN 505
TFHSDVVVD VCYRRFGHN
Sbjct: 363 TFHSDVVVDFVCYRRFGHN 419
>BU551159 weakly similar to GP|28416717|gb At3g55410 {Arabidopsis thaliana},
partial (8%)
Length = 676
Score = 152 bits (384), Expect = 7e-37
Identities = 73/85 (85%), Positives = 77/85 (89%)
Frame = -3
Query: 939 ELKRYPNAEVVWCQEEPMNMGGYSYILPRLITSMKALGRGGYEDVKYVGRAPSAATATGF 998
ELKRYPNAEVVWCQEEPMNM Y+YILPRLI+SMKA GRGGYEDVKYVGRA SAATATGF
Sbjct: 674 ELKRYPNAEVVWCQEEPMNMXXYTYILPRLISSMKAXGRGGYEDVKYVGRAXSAATATGF 495
Query: 999 LKVHQREQAELVHKAMQHKPINFPY 1023
LKVH EQAELV KA+Q +PINFPY
Sbjct: 494 LKVHLNEQAELVQKAIQREPINFPY 420
>TC228024 weakly similar to UP|O49541 (O49541) Oxoglutarate dehydrogenase -
like protein , partial (6%)
Length = 454
Score = 111 bits (277), Expect = 2e-24
Identities = 50/65 (76%), Positives = 60/65 (91%)
Frame = +2
Query: 959 GGYSYILPRLITSMKALGRGGYEDVKYVGRAPSAATATGFLKVHQREQAELVHKAMQHKP 1018
GGY+YILPRLI+SMKA+ RGGY+DVKYVGRAPSAATATGFLKVHQ+EQ E+ KA+Q +P
Sbjct: 2 GGYTYILPRLISSMKAVNRGGYDDVKYVGRAPSAATATGFLKVHQKEQTEIAEKALQQEP 181
Query: 1019 INFPY 1023
I+FP+
Sbjct: 182 IDFPF 196
>CA800991 similar to GP|10177333|db 2-oxoglutarate dehydrogenase E1
component {Arabidopsis thaliana}, partial (1%)
Length = 441
Score = 53.1 bits (126), Expect = 6e-07
Identities = 30/55 (54%), Positives = 35/55 (63%), Gaps = 8/55 (14%)
Frame = +2
Query: 1 MAWFRAAALSIAKHAIRRNLFRGGSSYYVTRTTNLPST--------SRKLHTTIF 47
MAWFRA SIAKHAIRR L +GGS+Y V+R LPS SR H+T+F
Sbjct: 278 MAWFRAGT-SIAKHAIRRTLSKGGSTYLVSRARFLPSIPSSSSSPYSRSFHSTVF 439
>TC206696 similar to UP|O24457 (O24457) Pyruvate dehydrogenase E1 alpha
subunit (At1g01090/T25K16_8) , partial (43%)
Length = 765
Score = 39.3 bits (90), Expect = 0.009
Identities = 35/133 (26%), Positives = 53/133 (39%), Gaps = 2/133 (1%)
Frame = +3
Query: 429 IHIVFNNQVAFTTDPESGRSSQYSTDVAKALNAPIFHVNGDDVESVVHVCELAAEWRQTF 488
+ +V NN A S A P HV+G DV V V + A +
Sbjct: 18 VFVVENNLWAIGMSHLRATSDPQIWKKGPAFGMPGVHVDGMDVLQVREVAKEAVGRARRV 197
Query: 489 HSDVVVDLVCYRRFGHNEIDEPSFTQP--KMYKVIRNHPSALEIYQKKLLELGELTQEDI 546
+V+ YR GH+ D P K + +R+ +AL+ Y L E ++++
Sbjct: 198 EGPTLVECETYRFRGHSLADPDELRDPAEKEHYAVRDPITALKKY---LFENNLANEQEL 368
Query: 547 DKIHKKVTSILND 559
I KK+ IL D
Sbjct: 369 KTIEKKIDEILED 407
>TC206697 similar to UP|O24457 (O24457) Pyruvate dehydrogenase E1 alpha
subunit (At1g01090/T25K16_8) , partial (33%)
Length = 566
Score = 36.2 bits (82), Expect = 0.075
Identities = 31/110 (28%), Positives = 50/110 (45%), Gaps = 4/110 (3%)
Frame = +3
Query: 465 HVNGDDVESVVHVCELAAEWRQTFHSDVVVDLVCYRRFGHNEIDEPSFTQP--KMYKVIR 522
HV+G DV V V + A E + +V+ YR GH+ D P K + R
Sbjct: 3 HVDGMDVLKVREVAKEAIERARRGEGPTLVECETYRFRGHSLADPDELRDPAEKAHYAGR 182
Query: 523 NHPSALEIYQKKLLELGELTQEDIDKIHKKVTSILND--EFLASKDYIPK 570
+ SAL+ Y ++E ++++ I KK+ I+ D EF + P+
Sbjct: 183 DPISALKKY---MIENKLASEQEFKTIEKKIEEIVEDAVEFADESPHSPR 323
>TC217476 similar to UP|O63201 (O63201) Cytochrome oxidase I (Fragment),
partial (12%)
Length = 603
Score = 31.2 bits (69), Expect = 2.4
Identities = 34/123 (27%), Positives = 52/123 (41%), Gaps = 1/123 (0%)
Frame = +3
Query: 14 HAIRRNLFRGGSSYYVTRTTNLPSTSRKLHTTIFKSEAHAAPVPRPVPLSRLTDNFLDGT 73
H IRR L R GS + L S R+ + + APVP P+PL ++ +FL
Sbjct: 36 HVIRRGLCRSGS-HECRHHRPLRSVLRRRR--LLAAPPLTAPVPPPLPLPQIYPHFLQFL 206
Query: 74 S-SAYLEELQRAWEADPSSVDESWDNFFRNFVGQASTSPGISGQTIQESMRLLLLVRAYQ 132
S L +L R + + S+ RN + + IS SM ++ V ++
Sbjct: 207 SPQIQLRQLSR--QQQQKRLSRSFSPSLRNILPLSYVEA*ISTGV---SMEIVYTVNSFV 371
Query: 133 VNG 135
NG
Sbjct: 372 QNG 380
>TC223633 weakly similar to UP|Q93VQ6 (Q93VQ6) At1g07080/F10K1_15, partial
(50%)
Length = 732
Score = 30.4 bits (67), Expect = 4.1
Identities = 17/28 (60%), Positives = 18/28 (63%), Gaps = 4/28 (14%)
Frame = -1
Query: 363 ANPSHLEAVNPLVV----GKTRAKQYYS 386
A PSHLEAVNPLV+ GKT Y S
Sbjct: 369 AQPSHLEAVNPLVMSPL*GKTLQTPYKS 286
>TC218033 similar to UP|Q9FFJ5 (Q9FFJ5) Gb|AAD32776.1 (At5g05830), partial
(61%)
Length = 1053
Score = 30.0 bits (66), Expect = 5.4
Identities = 14/39 (35%), Positives = 25/39 (63%)
Frame = +3
Query: 646 LAFATLIVEGNHVRLSGQDVERGTFSHRHAVVHDQATGE 684
L FA + + + +++S D+E+G +HRH+VV +GE
Sbjct: 144 LCFALVG*KMSTLQMSRVDLEQGNVNHRHSVVGSDVSGE 260
>TC228841
Length = 584
Score = 29.3 bits (64), Expect = 9.2
Identities = 24/79 (30%), Positives = 33/79 (41%), Gaps = 7/79 (8%)
Frame = -2
Query: 112 GISGQTIQESMRLLLLVRAYQV-------NGHMKAKLDPLGLKERNIPDELDPGLYGFTE 164
GI G TI S L L R+ ++ + +K LDP L+ IP+E
Sbjct: 280 GIPGPTIDHSSINLSLSRSLRIFSLHALESDDLKRFLDPFSLRPSEIPNESSSSTLSVGG 101
Query: 165 ADLDREFFLGVWNMSGFLS 183
D D+E G + G LS
Sbjct: 100 EDEDQEKLGGSRVVRGMLS 44
>BF595497
Length = 366
Score = 29.3 bits (64), Expect = 9.2
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = +1
Query: 25 SSYYVTRTTNLPSTSRKLHTTIFKSEAHAAPVPRPVPLSRLTD 67
S+Y++TR L S T K H + VP P SRL D
Sbjct: 22 SNYFITRIIPLTSLQDYQRITELKRRGHNSIVPSPTITSRLKD 150
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.137 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,764,625
Number of Sequences: 63676
Number of extensions: 598149
Number of successful extensions: 2577
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 2541
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2576
length of query: 1023
length of database: 12,639,632
effective HSP length: 107
effective length of query: 916
effective length of database: 5,826,300
effective search space: 5336890800
effective search space used: 5336890800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0180a.12