BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0177.9
(113 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC207638 UP|RPC2_SOYBN (Q8HVY3) DNA-directed RNA polymerase beta... 198 4e-52
TC208515 weakly similar to UP|Q9FNL5 (Q9FNL5) Similarity to guan... 29 0.29
BQ094303 PIR|S63667|S63 phragmoplastin 12 - soybean, partial (13%) 29 0.29
TC220780 weakly similar to UP|Q9LVH0 (Q9LVH0) DNA-directed RNA p... 28 0.84
BG839610 similar to GP|1174336|emb glucose-6-phosphate 1-dehydro... 27 1.4
AW567823 26 3.2
BG882711 25 4.2
TC234566 25 4.2
TC229604 similar to UP|Q9M276 (Q9M276) Homeobox-leucine zipper p... 25 7.1
AW568201 similar to PIR|S56674|S566 annexin homolog RJ4 (clone R... 25 7.1
TC207100 UP|Q39828 (Q39828) SDL5A, complete 25 7.1
TC220702 UP|Q39821 (Q39821) SDL12A, complete 25 7.1
BG239595 24 9.3
TC220164 weakly similar to UP|Q6KA57 (Q6KA57) Lipase class 3-lik... 24 9.3
TC220834 similar to UP|Q9LWR7 (Q9LWR7) ESTs D41826(S4655), parti... 24 9.3
>TC207638 UP|RPC2_SOYBN (Q8HVY3) DNA-directed RNA polymerase beta'' chain
(PEP) (Plastid-encoded RNA polymerase beta'' subunit)
(RNA polymerase beta'' subunit) , complete
Length = 4156
Score = 198 bits (503), Expect = 4e-52
Identities = 102/113 (90%), Positives = 109/113 (96%)
Frame = +2
Query: 1 AQSRISLVNKIQRVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELIGLLRA 60
AQSRISLVNKIQ+VYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVF GELIGLLRA
Sbjct: 3599 AQSRISLVNKIQKVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFLPGELIGLLRA 3778
Query: 61 QRTGRALEESICYRTILLGITKTSMNTQSFISEASFQETARVLAKAALRGRID 113
+R GR+LEE+ICYR +LLGITKTS+NTQSFISEASFQETARVL+KAALRGRID
Sbjct: 3779 ERAGRSLEEAICYRVLLLGITKTSLNTQSFISEASFQETARVLSKAALRGRID 3937
>TC208515 weakly similar to UP|Q9FNL5 (Q9FNL5) Similarity to guanylate
binding protein, partial (3%)
Length = 705
Score = 29.3 bits (64), Expect = 0.29
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +1
Query: 16 RSQGVHIHNRHIEIIVRQITSKVLVSE 42
R QGVH HN+ + + ++ K+LVSE
Sbjct: 190 REQGVHTHNQKMVVPSLRVLKKILVSE 270
>BQ094303 PIR|S63667|S63 phragmoplastin 12 - soybean, partial (13%)
Length = 423
Score = 29.3 bits (64), Expect = 0.29
Identities = 20/78 (25%), Positives = 37/78 (46%), Gaps = 1/78 (1%)
Frame = +3
Query: 2 QSRISLVNKIQRVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELIGL-LRA 60
++ ISLVNKIQR + G H N + + + + +V L ++G
Sbjct: 102 ENLISLVNKIQRACTALGDHGENSALPTLWDSLPAIAVVGGQSSGKSSVLESVVGKDFLP 281
Query: 61 QRTGRALEESICYRTILL 78
+ +GR S+C +++L+
Sbjct: 282 RGSGRRRSLSVCLKSMLV 335
>TC220780 weakly similar to UP|Q9LVH0 (Q9LVH0) DNA-directed RNA polymerase II
largest chain, partial (12%)
Length = 1049
Score = 27.7 bits (60), Expect = 0.84
Identities = 15/39 (38%), Positives = 26/39 (66%), Gaps = 2/39 (5%)
Frame = +3
Query: 77 LLGITKTSMNT--QSFISEASFQETARVLAKAALRGRID 113
+LGI + ++ +S + ASF+ TA +L +A++RGR D
Sbjct: 396 ILGINRFGISKMGKSVLMLASFERTADILFQASVRGRDD 512
>BG839610 similar to GP|1174336|emb glucose-6-phosphate 1-dehydrogenase
{Arabidopsis thaliana}, partial (22%)
Length = 529
Score = 26.9 bits (58), Expect = 1.4
Identities = 11/26 (42%), Positives = 18/26 (68%)
Frame = -2
Query: 52 GELIGLLRAQRTGRALEESICYRTIL 77
G ++ L+ R GRALE+S C++ +L
Sbjct: 303 GNVLQLVLCYRRGRALEQSSCFQFLL 226
>AW567823
Length = 426
Score = 25.8 bits (55), Expect = 3.2
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -1
Query: 21 HIHNRHIEIIVRQI 34
HIH RHI II+R I
Sbjct: 183 HIHGRHITIIIRAI 142
>BG882711
Length = 402
Score = 25.4 bits (54), Expect = 4.2
Identities = 8/25 (32%), Positives = 15/25 (60%)
Frame = -1
Query: 7 LVNKIQRVYRSQGVHIHNRHIEIIV 31
L++ + Y + VH+H R I I++
Sbjct: 168 LISCFESAYSNSSVHVHRRKISIVI 94
>TC234566
Length = 578
Score = 25.4 bits (54), Expect = 4.2
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = +1
Query: 20 VHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELIGLLRAQRTGRALEESIC 72
VH+ +HI + + Q +VL+SE V SL L Q TG A ES+C
Sbjct: 118 VHLAEQHISMDITQGIREVLLSEAFSGPVSSL-HLFEKPTDQHTGSA-TESVC 270
>TC229604 similar to UP|Q9M276 (Q9M276) Homeobox-leucine zipper protein
ATHB-12, partial (44%)
Length = 992
Score = 24.6 bits (52), Expect = 7.1
Identities = 12/35 (34%), Positives = 21/35 (59%)
Frame = -2
Query: 1 AQSRISLVNKIQRVYRSQGVHIHNRHIEIIVRQIT 35
+Q IS++NKIQ+ V + + + +I+RQ T
Sbjct: 775 SQGTISMLNKIQKAMFIF*VKVFHLYASVIIRQNT 671
>AW568201 similar to PIR|S56674|S566 annexin homolog RJ4 (clone RJ4) - garden
strawberry (fragment), partial (29%)
Length = 379
Score = 24.6 bits (52), Expect = 7.1
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +2
Query: 20 VHIHNRHIEIIVRQITSKVLVSEDGMSNV 48
++ HN+ E +VR +K +DG+S V
Sbjct: 155 INDHNKFYEKVVRNAINKFGTDDDGLSRV 241
>TC207100 UP|Q39828 (Q39828) SDL5A, complete
Length = 2284
Score = 24.6 bits (52), Expect = 7.1
Identities = 15/55 (27%), Positives = 25/55 (45%)
Frame = +2
Query: 2 QSRISLVNKIQRVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELIG 56
++ ISLVNKIQR + G H N + + + + +V L ++G
Sbjct: 155 ENLISLVNKIQRACTALGDHGENSALPTLWDSLPAIAVVGGQSSGKSSVLESVVG 319
>TC220702 UP|Q39821 (Q39821) SDL12A, complete
Length = 2213
Score = 24.6 bits (52), Expect = 7.1
Identities = 15/55 (27%), Positives = 25/55 (45%)
Frame = +1
Query: 2 QSRISLVNKIQRVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELIG 56
++ ISLVNKIQR + G H N + + + + +V L ++G
Sbjct: 178 ENLISLVNKIQRACTALGDHGENSALPTLWDSLPAIAVVGGQSSGKSSVLESVVG 342
>BG239595
Length = 232
Score = 24.3 bits (51), Expect = 9.3
Identities = 16/44 (36%), Positives = 23/44 (51%)
Frame = +3
Query: 16 RSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELIGLLR 59
RSQ +H HNR ++ R I ++ + G SL + GLLR
Sbjct: 24 RSQRLHRHNRSTSLLHRLILARDTLCLTG--G*HSLTPIFGLLR 149
>TC220164 weakly similar to UP|Q6KA57 (Q6KA57) Lipase class 3-like, partial
(49%)
Length = 596
Score = 24.3 bits (51), Expect = 9.3
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +3
Query: 18 QGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLGELIGLLRAQRTGRALEES 70
QG+H +R EI ++ + + V D SNV+ G +G A TG+ + ++
Sbjct: 270 QGLHQTSRS-EIAIQAVQNTVATVGD--SNVWLAGHSLGSAMAMLTGKTMAKN 419
>TC220834 similar to UP|Q9LWR7 (Q9LWR7) ESTs D41826(S4655), partial (54%)
Length = 576
Score = 24.3 bits (51), Expect = 9.3
Identities = 10/41 (24%), Positives = 21/41 (50%)
Frame = -3
Query: 12 QRVYRSQGVHIHNRHIEIIVRQITSKVLVSEDGMSNVFSLG 52
+++ +++ +HN+HI + V V S + N+ LG
Sbjct: 352 KKILKTRSQQVHNKHIILPVYAAPFYVRDSTSSLKNIVELG 230
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.134 0.352
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,356,625
Number of Sequences: 63676
Number of extensions: 29564
Number of successful extensions: 141
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of query: 113
length of database: 12,639,632
effective HSP length: 89
effective length of query: 24
effective length of database: 6,972,468
effective search space: 167339232
effective search space used: 167339232
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0177.9