BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0175.3
(520 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229525 similar to UP|Q8VXU8 (Q8VXU8) AT5g23690/MQM1_4, partial... 318 3e-87
CO984589 208 4e-54
AI900305 147 9e-36
BG352266 similar to GP|18377862|gb| AT5g23690/MQM1_4 {Arabidopsi... 95 9e-20
AW472374 weakly similar to GP|18377862|gb| AT5g23690/MQM1_4 {Ara... 87 1e-17
AI960816 homologue to EGAD|134728|143 lipoxygenase {Phaseolus vu... 30 2.0
TC216572 30 3.4
TC219545 similar to UP|Q8W4E1 (Q8W4E1) ATP-dependent RNA helicas... 30 3.4
TC222927 homologue to UP|Q9M5A6 (Q9M5A6) Unconventional myosin X... 29 4.4
TC232934 weakly similar to UP|Q7RC64 (Q7RC64) Immediate early pr... 29 4.4
TC210833 29 5.8
TC229072 28 7.6
TC204086 similar to UP|Q8H2B9 (Q8H2B9) 60s acidic ribosomal prot... 28 7.6
TC226260 similar to UP|O22285 (O22285) Expressed protein (At2g39... 28 9.9
TC204024 28 9.9
>TC229525 similar to UP|Q8VXU8 (Q8VXU8) AT5g23690/MQM1_4, partial (25%)
Length = 1107
Score = 318 bits (816), Expect = 3e-87
Identities = 165/250 (66%), Positives = 186/250 (74%), Gaps = 25/250 (10%)
Frame = +3
Query: 295 RFGLLDILLPFQAVYFVHHRFRRRDKRTNMLL-------------------------SLF 329
RFGLLDILLPFQA YFV FRRRDKRTN+LL S F
Sbjct: 9 RFGLLDILLPFQAAYFVRGGFRRRDKRTNLLLVMNH*RHPSH*RDKRPSSSEKLMEGSFF 188
Query: 330 SNLDKLLAPNRPCHSSLWVGILALHKTLSDQPRNPLVVAAFSLAVHNGGNLLEAVDISRR 389
SN+DKLLAPNRPCHSSLWV ILALHK LSD+PR+ VVAAFSLA+HNGGNL EA+ I+RR
Sbjct: 189 SNVDKLLAPNRPCHSSLWVVILALHKALSDKPRDSSVVAAFSLALHNGGNLSEAISIARR 368
Query: 390 INQPHDDRFPELLDPSDLEAEALADEILDLAESVKESLLQMTTRHMVSQAMADYPQAPHS 449
IN+ HD RFPELLDPS L+ E L EILDLAESVK SLLQM RH VS AMADYPQAPHS
Sbjct: 369 INKTHDARFPELLDPSGLDEEDLEAEILDLAESVKGSLLQMKDRHFVSGAMADYPQAPHS 548
Query: 450 DMVFIPLGMYLKTVSIFDCLRARAGRKFLSKQDRKTDYESLAHGDLQEVRHMLAMVVFDT 509
D+V IP+ MYL+ ++ FDC+R A + FLSKQ R+ DYESLA GDL EVRH+ A +VFDT
Sbjct: 549 DLVLIPIPMYLRALNFFDCVRVSASKNFLSKQGRQIDYESLARGDLLEVRHVFARIVFDT 728
Query: 510 IYPLHLDRDQ 519
+YPLH +DQ
Sbjct: 729 VYPLHPGKDQ 758
>CO984589
Length = 778
Score = 208 bits (530), Expect = 4e-54
Identities = 103/154 (66%), Positives = 128/154 (82%)
Frame = -2
Query: 367 VAAFSLAVHNGGNLLEAVDISRRINQPHDDRFPELLDPSDLEAEALADEILDLAESVKES 426
VAAFSLAVHNGGNLLEAV ++ IN+PHD RFPELLDPS L+AEAL EILDLAESV+ +
Sbjct: 777 VAAFSLAVHNGGNLLEAVSMAGMINKPHDVRFPELLDPSGLDAEALEAEILDLAESVRGT 598
Query: 427 LLQMTTRHMVSQAMADYPQAPHSDMVFIPLGMYLKTVSIFDCLRARAGRKFLSKQDRKTD 486
+LQMT + VSQAMADYPQAP S++VFIP+ +Y+K ++FDC+R +KFLSKQ RK D
Sbjct: 597 ILQMTNEYFVSQAMADYPQAPRSNLVFIPITLYVKVYNMFDCVRRSTVKKFLSKQHRKID 418
Query: 487 YESLAHGDLQEVRHMLAMVVFDTIYPLHLDRDQS 520
Y+SLA G+LQEVRH+ A +VFDT+YPL +++QS
Sbjct: 417 YQSLALGNLQEVRHVFARIVFDTVYPLCPNQNQS 316
>AI900305
Length = 427
Score = 147 bits (372), Expect = 9e-36
Identities = 77/102 (75%), Positives = 84/102 (81%)
Frame = +3
Query: 348 VGILALHKTLSDQPRNPLVVAAFSLAVHNGGNLLEAVDISRRINQPHDDRFPELLDPSDL 407
V ILALHK LSD+PR+ VVAAFSLA+HNGGNL EA+ I+RRIN+ HD RFPELLDPS L
Sbjct: 120 VVILALHKALSDKPRDSSVVAAFSLALHNGGNLSEAISIARRINKTHDARFPELLDPSGL 299
Query: 408 EAEALADEILDLAESVKESLLQMTTRHMVSQAMADYPQAPHS 449
+ E L EILDLAESVK SLLQM RH VS AMADYPQAPHS
Sbjct: 300 DEEDLEAEILDLAESVKGSLLQMKDRHFVSGAMADYPQAPHS 425
>BG352266 similar to GP|18377862|gb| AT5g23690/MQM1_4 {Arabidopsis thaliana},
partial (10%)
Length = 403
Score = 94.7 bits (234), Expect = 9e-20
Identities = 46/61 (75%), Positives = 51/61 (83%)
Frame = +3
Query: 53 NKKTPAWKKLNSKELGLRNSMIAHPTKRVLSTLKKKGYDVYLVGGCVRDLILKQTPKDFD 112
N K AWKKLNSKE+GLRNSMIA PTK+VL+ LKKKG+DVYLVG CVR+LIL KDFD
Sbjct: 93 NSKVRAWKKLNSKEIGLRNSMIAIPTKKVLNVLKKKGHDVYLVGRCVRELILXGNTKDFD 272
Query: 113 I 113
I
Sbjct: 273 I 275
>AW472374 weakly similar to GP|18377862|gb| AT5g23690/MQM1_4 {Arabidopsis
thaliana}, partial (6%)
Length = 443
Score = 87.4 bits (215), Expect = 1e-17
Identities = 40/69 (57%), Positives = 55/69 (78%)
Frame = +1
Query: 452 VFIPLGMYLKTVSIFDCLRARAGRKFLSKQDRKTDYESLAHGDLQEVRHMLAMVVFDTIY 511
VFIP+ +Y+K ++FDC+R +KFLSKQ RK DY+SLA G+LQEVRH+ A +VFDT+Y
Sbjct: 139 VFIPITLYVKVYNMFDCVRRSTVKKFLSKQHRKIDYQSLALGNLQEVRHVFARIVFDTVY 318
Query: 512 PLHLDRDQS 520
PL +++QS
Sbjct: 319 PLCPNQNQS 345
>AI960816 homologue to EGAD|134728|143 lipoxygenase {Phaseolus vulgaris},
partial (10%)
Length = 203
Score = 30.4 bits (67), Expect = 2.0
Identities = 15/39 (38%), Positives = 25/39 (63%), Gaps = 1/39 (2%)
Frame = +3
Query: 7 LKFVCKPNLHLRIAPFQCVRKVSQLKLNLNHT-HHLASS 44
++F+ +++AP+QC RK S L +N + + HHL SS
Sbjct: 33 VEFISLQMHSVKLAPYQCSRKSSALMVNKSSSFHHLMSS 149
>TC216572
Length = 1368
Score = 29.6 bits (65), Expect = 3.4
Identities = 11/13 (84%), Positives = 12/13 (91%)
Frame = +3
Query: 45 FISVCFPQNKKTP 57
FISVCFP NK+TP
Sbjct: 1257 FISVCFPSNKRTP 1295
>TC219545 similar to UP|Q8W4E1 (Q8W4E1) ATP-dependent RNA helicase-like
protein, partial (48%)
Length = 1075
Score = 29.6 bits (65), Expect = 3.4
Identities = 14/30 (46%), Positives = 19/30 (62%)
Frame = -3
Query: 440 MADYPQAPHSDMVFIPLGMYLKTVSIFDCL 469
+A +P++P + M FIP LKT S F CL
Sbjct: 596 LASFPRSPCNSMAFIPRASSLKTTS-FSCL 510
>TC222927 homologue to UP|Q9M5A6 (Q9M5A6) Unconventional myosin XI, partial
(12%)
Length = 718
Score = 29.3 bits (64), Expect = 4.4
Identities = 11/42 (26%), Positives = 24/42 (56%)
Frame = +2
Query: 7 LKFVCKPNLHLRIAPFQCVRKVSQLKLNLNHTHHLASSFISV 48
+K VC + H+++ P C+R +K + N + H+ +S ++
Sbjct: 215 MKLVCFLDQHMKLLPKSCIRHSKTIKGSANPSWHVVTSQFAI 340
>TC232934 weakly similar to UP|Q7RC64 (Q7RC64) Immediate early protein
homolog (Fragment), partial (3%)
Length = 761
Score = 29.3 bits (64), Expect = 4.4
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +2
Query: 340 RPCHSSLWVGILALHKTLSDQPRNPLV 366
RP H S+W+ + LHKT S++P PL+
Sbjct: 293 RPRHKSVWLWFVNLHKT-SEKPWKPLL 370
>TC210833
Length = 608
Score = 28.9 bits (63), Expect = 5.8
Identities = 20/77 (25%), Positives = 36/77 (45%), Gaps = 1/77 (1%)
Frame = -1
Query: 301 ILLPFQAVYFVHHRFRRRDKRTNMLLSLFSNLDKLLAPNRPCHSSLWVGILALHKTLS-D 359
+++ + ++ HH R N+LLS S+L L N CH + + H+ S +
Sbjct: 362 LIVGLRNIWVSHHTVRNIWP*LNLLLSRLSSLKHFLWLNISCHCCFLLLQVHFHRLSSFN 183
Query: 360 QPRNPLVVAAFSLAVHN 376
+N L +A + VH+
Sbjct: 182 TKKNMLDLACTAFTVHS 132
>TC229072
Length = 1166
Score = 28.5 bits (62), Expect = 7.6
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 5/64 (7%)
Frame = +3
Query: 29 SQLKLNLNHTHHLASSFISVCFPQNKKTPAWK-----KLNSKELGLRNSMIAHPTKRVLS 83
S LNL+ L+S + FPQN TP++ +N + + L+ + P RV++
Sbjct: 93 SMATLNLSSLQALSSHRLCSSFPQNTPTPSFNFQLKPSINPRPISLKPYVSEKPRARVVA 272
Query: 84 TLKK 87
K
Sbjct: 273 LAVK 284
>TC204086 similar to UP|Q8H2B9 (Q8H2B9) 60s acidic ribosomal protein,
complete
Length = 907
Score = 28.5 bits (62), Expect = 7.6
Identities = 28/93 (30%), Positives = 41/93 (43%), Gaps = 4/93 (4%)
Frame = +1
Query: 356 TLSDQPRNPLVVAAFSLAVHNGGNLLEAVDISRRIN----QPHDDRFPELLDPSDLEAEA 411
TLS R VVAA+ LAV G N A DI + + +DR L +E
Sbjct: 349 TLSLSLRKMKVVAAYLLAVLGGNNTPSADDIKEILGSVGIEADEDRIESFL------SEV 510
Query: 412 LADEILDLAESVKESLLQMTTRHMVSQAMADYP 444
+I++L + KE L + + + A+A P
Sbjct: 511 KGKDIVELIAAGKEKLASVPSGGGGAVAVAAAP 609
>TC226260 similar to UP|O22285 (O22285) Expressed protein (At2g39750/T5I7.5),
partial (79%)
Length = 2458
Score = 28.1 bits (61), Expect = 9.9
Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 5/80 (6%)
Frame = -2
Query: 44 SFISVCFPQNKKTPAWKKLNSKELGLRNSMIAHPTKRVLSTLKKKG---YDVYLVG--GC 98
S +V FPQ+ TP+ K SK + + P + ++S G ++ +++G
Sbjct: 240 SSTTVSFPQSSGTPSSKCPISKSSDMVPLSLRIPKRLIVSRSSGGGGGCWEGFVLGVWAS 61
Query: 99 VRDLILKQTPKDFDILTSAD 118
+R L LK K D++TSAD
Sbjct: 60 IRALTLKNWLKLGDMVTSAD 1
>TC204024
Length = 423
Score = 28.1 bits (61), Expect = 9.9
Identities = 20/72 (27%), Positives = 36/72 (49%), Gaps = 7/72 (9%)
Frame = -3
Query: 162 TRFVHDIEAPNGCDNEDFLRWKNCL-------NRDFTINGLMFDPYAKIVYDYMGGMEDI 214
++F HDIE PNG +N +NC+ + D T+NG + ++V+ G +
Sbjct: 205 SQFRHDIEIPNG-ENA-----RNCVANMILKFHDDPTVNGFEIVVFLRLVWWAAGKRKGF 44
Query: 215 VKAKVRTVVPAA 226
+ + +T +P A
Sbjct: 43 ERRRGKTKMPRA 8
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.138 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,580,638
Number of Sequences: 63676
Number of extensions: 335745
Number of successful extensions: 2209
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 2192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2208
length of query: 520
length of database: 12,639,632
effective HSP length: 102
effective length of query: 418
effective length of database: 6,144,680
effective search space: 2568476240
effective search space used: 2568476240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0175.3