BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0150.6
(396 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC217086 similar to UP|Q940Y3 (Q940Y3) At2g17400 (At2g17400/At2g... 270 1e-72
TC209945 196 1e-50
BG155228 similar to GP|15450515|gb At2g17400 {Arabidopsis thalia... 182 2e-46
TC219620 similar to UP|Q6ZFT8 (Q6ZFT8) DNA-binding protein-like,... 176 1e-44
TC217087 similar to UP|Q7XJS4 (Q7XJS4) At2g17410 protein, partia... 162 2e-40
AW234357 homologue to GP|15450515|gb| At2g17400 {Arabidopsis tha... 98 6e-21
TC218291 similar to GB|AAM91387.1|22137084|AY133557 At3g13350/MD... 33 0.23
TC220012 similar to UP|O82305 (O82305) Expressed protein (At2g25... 31 1.1
TC208244 similar to UP|Q94JV1 (Q94JV1) At1g69340/F10D13.28, part... 30 1.5
TC204530 30 1.5
TC225535 homologue to UP|HS16_SOYBN (P05478) 18.5 kDa class I he... 30 1.9
TC225537 UP|HS15_SOYBN (P04795) 17.6 kDa class I heat shock prot... 29 4.2
TC219080 similar to UP|Q9LUT4 (Q9LUT4) Gb|AAF34859.1, partial (45%) 29 4.2
TC225541 homologue to UP|HS13_SOYBN (P04793) 17.5 kDa class I he... 28 5.5
TC225534 homologue to UP|HS13_SOYBN (P04793) 17.5 kDa class I he... 28 7.2
BM309555 28 7.2
TC229069 28 9.5
TC210431 28 9.5
TC229070 28 9.5
>TC217086 similar to UP|Q940Y3 (Q940Y3) At2g17400 (At2g17400/At2g17400),
partial (24%)
Length = 949
Score = 270 bits (689), Expect = 1e-72
Identities = 138/220 (62%), Positives = 162/220 (72%), Gaps = 31/220 (14%)
Frame = +3
Query: 204 YEKALLEYEKHKREIGELQLPVGVFPQPSSVEKETTVYQAPGSGRARRDAAARAMQGWHA 263
YEKALL+YE+HK + GEL +PV P+P ++E QA SGRARRDAAARAMQGWH+
Sbjct: 3 YEKALLDYERHKIKGGELNVPVASHPEPINIEN-----QASASGRARRDAAARAMQGWHS 167
Query: 264 QRLLGYGEVAEPVIKDKNFNPTTKREKNLKSI---------------------------- 295
QRLLG GEV++P+IKD+N KREK LKSI
Sbjct: 168 QRLLGNGEVSDPIIKDRNSVSVQKREKQLKSINIHKRKKPSSPYMDNAVKAARSKPSKPQ 347
Query: 296 ---AVVDVGPPADWVKINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITGMPEHID 352
V+D+GPPADWVK+NV++TKDCFEVYALVPGLLREEVRVQSDP GRLVI+G PEH +
Sbjct: 348 LDTTVIDIGPPADWVKVNVQKTKDCFEVYALVPGLLREEVRVQSDPAGRLVISGEPEHPN 527
Query: 353 NPWGITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFE 392
NPWG+TPFKKVV+LP+RIDP QTSAVV+LHG+LFVRVPFE
Sbjct: 528 NPWGVTPFKKVVSLPSRIDPHQTSAVVTLHGQLFVRVPFE 647
>TC209945
Length = 882
Score = 196 bits (499), Expect = 1e-50
Identities = 96/101 (95%), Positives = 98/101 (96%)
Frame = +1
Query: 296 AVVDVGPPADWVKINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITGMPEHIDNPW 355
AVVDVGPPADWVKINVRETKD FEVYALVPGLLREEVRVQSDPVGRLVITG+PEH+DNPW
Sbjct: 196 AVVDVGPPADWVKINVRETKDGFEVYALVPGLLREEVRVQSDPVGRLVITGVPEHLDNPW 375
Query: 356 GITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFEQGAV 396
GITPFKKVVNLP RID LQTSAVVSLHGRLFVRVPFEQGAV
Sbjct: 376 GITPFKKVVNLPTRIDSLQTSAVVSLHGRLFVRVPFEQGAV 498
>BG155228 similar to GP|15450515|gb At2g17400 {Arabidopsis thaliana}, partial
(12%)
Length = 500
Score = 182 bits (462), Expect = 2e-46
Identities = 85/117 (72%), Positives = 102/117 (86%)
Frame = +2
Query: 276 VIKDKNFNPTTKREKNLKSIAVVDVGPPADWVKINVRETKDCFEVYALVPGLLREEVRVQ 335
V+K + P+ + +++ +VVD+GPPADWVKINV++TKDCFEVYALVPGLLREEVRVQ
Sbjct: 86 VVKVAHSQPSRPQLRHISDTSVVDIGPPADWVKINVQKTKDCFEVYALVPGLLREEVRVQ 265
Query: 336 SDPVGRLVITGMPEHIDNPWGITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFE 392
SDP GRLVI+G PEH DNPWG+TPFKKVV+LP+RID QTSAVV+LHG+LFVRVPFE
Sbjct: 266 SDPAGRLVISGEPEHSDNPWGVTPFKKVVSLPSRIDTQQTSAVVTLHGQLFVRVPFE 436
>TC219620 similar to UP|Q6ZFT8 (Q6ZFT8) DNA-binding protein-like, partial
(24%)
Length = 658
Score = 176 bits (447), Expect = 1e-44
Identities = 82/97 (84%), Positives = 92/97 (94%)
Frame = +2
Query: 296 AVVDVGPPADWVKINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITGMPEHIDNPW 355
+VVD+GPPADWVKINV++TKDCFEVYALVPGLLREEVRVQSDP GRLVI+G PEH DNPW
Sbjct: 113 SVVDIGPPADWVKINVQKTKDCFEVYALVPGLLREEVRVQSDPAGRLVISGEPEHSDNPW 292
Query: 356 GITPFKKVVNLPARIDPLQTSAVVSLHGRLFVRVPFE 392
G+TPFKKVV+LP+RID QTSAVV+LHG+LFVRVPFE
Sbjct: 293 GVTPFKKVVSLPSRIDTQQTSAVVTLHGQLFVRVPFE 403
>TC217087 similar to UP|Q7XJS4 (Q7XJS4) At2g17410 protein, partial (36%)
Length = 764
Score = 162 bits (411), Expect = 2e-40
Identities = 82/132 (62%), Positives = 95/132 (71%), Gaps = 25/132 (18%)
Frame = +1
Query: 286 TKREKNLKSIAVVDVGPPADWVKINVRET-------------------------KDCFEV 320
+K K V+D+GPPADWVK+NV++T KDCFEV
Sbjct: 58 SKPSKPQLDTTVIDIGPPADWVKVNVQKTVSSLSCSNK*VIYVIPYFNENSSVQKDCFEV 237
Query: 321 YALVPGLLREEVRVQSDPVGRLVITGMPEHIDNPWGITPFKKVVNLPARIDPLQTSAVVS 380
YALVPGLLREEVRVQSDP GRLVI+G PEH +NPWG+TPFKKVV+LP+RIDP QTSAVV+
Sbjct: 238 YALVPGLLREEVRVQSDPAGRLVISGEPEHPNNPWGVTPFKKVVSLPSRIDPHQTSAVVT 417
Query: 381 LHGRLFVRVPFE 392
LHG+LFVRVPFE
Sbjct: 418 LHGQLFVRVPFE 453
>AW234357 homologue to GP|15450515|gb| At2g17400 {Arabidopsis thaliana},
partial (7%)
Length = 409
Score = 98.2 bits (243), Expect = 6e-21
Identities = 52/104 (50%), Positives = 65/104 (62%)
Frame = +3
Query: 72 EVKTGSENQLELEDVKTPLHQELVTPKSRERNVREMKSVLNDTEVVDYDEPGASLEREAF 131
+ T N LE+E+ ++ P ++ + L+ D +E G E+ AF
Sbjct: 96 DTNTEIMNVLEVENKAD--EKQATEPADNGKSNSKYMLFLDADHSYDGNESGTEEEQSAF 269
Query: 132 MKELENFYRERSLEFKPPKFYGEPLNCLKLWRAVIRLGGYDVVT 175
MKELENF+RERS+EFKPPKFYGE LNCLKLWRAV RLGGYD VT
Sbjct: 270 MKELENFFRERSMEFKPPKFYGEGLNCLKLWRAVTRLGGYDKVT 401
>TC218291 similar to GB|AAM91387.1|22137084|AY133557 At3g13350/MDC11_14
{Arabidopsis thaliana;} , partial (22%)
Length = 697
Score = 33.1 bits (74), Expect = 0.23
Identities = 22/83 (26%), Positives = 38/83 (45%)
Frame = +3
Query: 131 FMKELENFYRERSLEFKPPKFYGEPLNCLKLWRAVIRLGGYDVVTGSKLWRQVGESFHPP 190
F L+ F++ ++K G L+ +L+ V GG + V + W++V +F+
Sbjct: 252 FWGTLQAFHKILGTKYKVATVGGTSLDLHRLFVEVTSRGGIEKVIVDRKWKEVILTFNFK 431
Query: 191 KTCTTVSWTFRIFYEKALLEYEK 213
T T+ S R Y L YE+
Sbjct: 432 DTITSASXVVRKSYLSMLYHYEQ 500
>TC220012 similar to UP|O82305 (O82305) Expressed protein
(At2g25920/F17H15.5), partial (47%)
Length = 1118
Score = 30.8 bits (68), Expect = 1.1
Identities = 20/73 (27%), Positives = 32/73 (43%), Gaps = 15/73 (20%)
Frame = +2
Query: 28 DQNAGSSPMVLAPNSL-AQIPKDEVMVDQGDDDNR--------------IGDSTPNNMLE 72
D N G SP +P++ + P++EV +D R +GDS P+N++E
Sbjct: 161 DSNLGGSPSASSPSAKRTRDPEEEVYLDNLRSHKRYLSEIMASSLNGLTVGDSLPDNLME 340
Query: 73 VKTGSENQLELED 85
SE+ D
Sbjct: 341 SPARSESMFSXRD 379
>TC208244 similar to UP|Q94JV1 (Q94JV1) At1g69340/F10D13.28, partial (37%)
Length = 917
Score = 30.4 bits (67), Expect = 1.5
Identities = 12/36 (33%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +1
Query: 243 APGSGRARR-DAAARAMQGWHAQRLLGYGEVAEPVI 277
AP R + + A A +GW+ +L+GYG++ P +
Sbjct: 1 APDQRRMEQWEKTAEAQRGWNCAKLIGYGDLGRPTL 108
>TC204530
Length = 957
Score = 30.4 bits (67), Expect = 1.5
Identities = 24/95 (25%), Positives = 36/95 (37%)
Frame = -2
Query: 155 PLNCLKLWRAVIRLGGYDVVTGSKLWRQVGESFHPPKTCTTVSWTFRIFYEKALLEYEKH 214
P+N +W A IR+ V+ G G P T ++S + R F AL H
Sbjct: 632 PVNWFPIWPASIRIAALSVLAGLP-----GSKMEPNSTNLSLSLSLRNFSRHALELEAAH 468
Query: 215 KREIGELQLPVGVFPQPSSVEKETTVYQAPGSGRA 249
++ P + P S E + G+ RA
Sbjct: 467 DSDLTLFFEPDSLRPSESPNEPSMSTPTLEGASRA 363
>TC225535 homologue to UP|HS16_SOYBN (P05478) 18.5 kDa class I heat shock
protein (HSP 18.5), complete
Length = 791
Score = 30.0 bits (66), Expect = 1.9
Identities = 29/97 (29%), Positives = 47/97 (47%), Gaps = 11/97 (11%)
Frame = +3
Query: 308 KINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITG----MPEHIDNPW-----GIT 358
+++ +ET + A +PGL +EEV+VQ + L I+G E +N W
Sbjct: 246 RVDWKETPEAHVFKADIPGLKKEEVKVQIEDDKVLQISGERNVEKEDRNNTWHRVERSSG 425
Query: 359 PFKKVVNLP--ARIDPLQTSAVVSLHGRLFVRVPFEQ 393
F + LP A++D ++ S +G L V VP E+
Sbjct: 426 KFMRRFRLPENAKVDKVKASME---NGVLTVTVPKEE 527
>TC225537 UP|HS15_SOYBN (P04795) 17.6 kDa class I heat shock protein (HSP
17.6-L), complete
Length = 617
Score = 28.9 bits (63), Expect = 4.2
Identities = 14/39 (35%), Positives = 26/39 (65%)
Frame = +2
Query: 308 KINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITG 346
+++ +ET++ + A +PGL +EEV+VQ + L I+G
Sbjct: 224 RVDWKETQEAHVLKADIPGLKKEEVKVQIEDDRVLQISG 340
>TC219080 similar to UP|Q9LUT4 (Q9LUT4) Gb|AAF34859.1, partial (45%)
Length = 1105
Score = 28.9 bits (63), Expect = 4.2
Identities = 25/80 (31%), Positives = 38/80 (47%), Gaps = 3/80 (3%)
Frame = +1
Query: 160 KLWRAVIRLGGYDVVT-GSKLWRQ--VGESFHPPKTCTTVSWTFRIFYEKALLEYEKHKR 216
++WR + G Y +VT G+ L+R + ES SWT ++ + L EK
Sbjct: 523 EIWRVLKDKGVYVLVTYGAPLYRLRLLQES---------CSWTIKLHVIEKLASEEKSDN 675
Query: 217 EIGELQLPVGVFPQPSSVEK 236
+ EL PV + SSVE+
Sbjct: 676 PVWELTKPVPLNDDGSSVEE 735
>TC225541 homologue to UP|HS13_SOYBN (P04793) 17.5 kDa class I heat shock
protein (HSP 17.5-M), complete
Length = 849
Score = 28.5 bits (62), Expect = 5.5
Identities = 24/69 (34%), Positives = 34/69 (48%)
Frame = +1
Query: 278 KDKNFNPTTKREKNLKSIAVVDVGPPADWVKINVRETKDCFEVYALVPGLLREEVRVQSD 337
KD +F PT+ +N S V DW +ET + A +PGL +EEV+VQ +
Sbjct: 82 KDFHF-PTSLSAENSAS-----VNTRVDW-----KETPEAHVFKADIPGLKKEEVKVQIE 228
Query: 338 PVGRLVITG 346
L I+G
Sbjct: 229 DDRVLQISG 255
>TC225534 homologue to UP|HS13_SOYBN (P04793) 17.5 kDa class I heat shock
protein (HSP 17.5-M), complete
Length = 594
Score = 28.1 bits (61), Expect = 7.2
Identities = 14/39 (35%), Positives = 25/39 (63%)
Frame = +2
Query: 308 KINVRETKDCFEVYALVPGLLREEVRVQSDPVGRLVITG 346
+++ +ET + + A +PGL +EEV+VQ + L I+G
Sbjct: 230 RVDWKETPEAHVLKADIPGLKKEEVKVQIEDDRVLQISG 346
>BM309555
Length = 435
Score = 28.1 bits (61), Expect = 7.2
Identities = 16/64 (25%), Positives = 35/64 (54%)
Frame = +1
Query: 60 NRIGDSTPNNMLEVKTGSENQLELEDVKTPLHQELVTPKSRERNVREMKSVLNDTEVVDY 119
N++ +S P+ + V + E+ + +TPL+ E TP+++ R+ KS++ + ++
Sbjct: 58 NQLVNSQPHYEI-VNASNIPSYEIVNAETPLNSEEPTPETQPSKRRKKKSIVWEHFTIET 234
Query: 120 DEPG 123
PG
Sbjct: 235 VSPG 246
>TC229069
Length = 526
Score = 27.7 bits (60), Expect = 9.5
Identities = 23/92 (25%), Positives = 39/92 (42%), Gaps = 7/92 (7%)
Frame = +3
Query: 3 GGEKPVDNMEIVDDDTTLLPQNSDKDQNAGSSPMVLAPNSLAQIPKDEVMVDQG------ 56
G P + IVD + PQ + Q S P V S++ + M++ G
Sbjct: 246 GNAAPSNYSSIVDPHS---PQGNGSPQ---SQPSVSTNRSISDVDSAVQMLEAGSGVTVH 407
Query: 57 -DDDNRIGDSTPNNMLEVKTGSENQLELEDVK 87
D D R+G+ + + +EN+ +LE+ K
Sbjct: 408 VDLDERVGEYVVKIKRSINSTAENEHKLEETK 503
>TC210431
Length = 422
Score = 27.7 bits (60), Expect = 9.5
Identities = 17/65 (26%), Positives = 35/65 (53%)
Frame = +3
Query: 59 DNRIGDSTPNNMLEVKTGSENQLELEDVKTPLHQELVTPKSRERNVREMKSVLNDTEVVD 118
D+ I +S P+ + V + E+ + +TPL+ E TP+++ R+ KS++ + ++
Sbjct: 69 DHPIVNSRPHYEI-VNASNIPSYEIINAETPLNSEEPTPETQPSKRRKKKSIVWEHFTIE 245
Query: 119 YDEPG 123
PG
Sbjct: 246 TVSPG 260
>TC229070
Length = 1026
Score = 27.7 bits (60), Expect = 9.5
Identities = 23/92 (25%), Positives = 39/92 (42%), Gaps = 7/92 (7%)
Frame = +2
Query: 3 GGEKPVDNMEIVDDDTTLLPQNSDKDQNAGSSPMVLAPNSLAQIPKDEVMVDQG------ 56
G P + IVD + PQ + Q S P V S++ + M++ G
Sbjct: 281 GNAAPSNYSSIVDPHS---PQGNGSPQ---SQPSVSTNRSISDVDSAVQMLEAGSGVTVH 442
Query: 57 -DDDNRIGDSTPNNMLEVKTGSENQLELEDVK 87
D D R+G+ + + +EN+ +LE+ K
Sbjct: 443 VDLDERVGEYVVKIKRSINSTAENEHKLEETK 538
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.315 0.135 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,680,129
Number of Sequences: 63676
Number of extensions: 235042
Number of successful extensions: 773
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 770
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 771
length of query: 396
length of database: 12,639,632
effective HSP length: 99
effective length of query: 297
effective length of database: 6,335,708
effective search space: 1881705276
effective search space used: 1881705276
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0150.6