BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0150.5
(168 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216013 homologue to UP|Q8L4X6 (Q8L4X6) WD-repeat protein GhTTG... 114 2e-26
TC216011 homologue to UP|Q8L4X6 (Q8L4X6) WD-repeat protein GhTTG... 72 3e-17
TC216015 similar to UP|Q9M610 (Q9M610) Ttg1-like protein, partia... 62 1e-10
TC216012 similar to UP|Q9M610 (Q9M610) Ttg1-like protein, partia... 52 1e-07
BG352594 similar to GP|22324809|gb| WD-repeat protein GhTTG4 {Go... 35 1e-04
TC204966 similar to UP|HMD1_ARATH (P14891) 3-hydroxy-3-methylglu... 32 0.14
TC219477 similar to UP|Q8LSC5 (Q8LSC5) Geranylgeranyl pyrophosph... 32 0.14
CA783163 similar to GP|8777474|dbj| contains similarity to rab3 ... 31 0.24
TC206672 weakly similar to UP|Q7UTL3 (Q7UTL3) 3-hydroxyisobutyra... 30 0.71
AW279280 similar to PIR|T04047|T0 isoleucine-tRNA ligase homolog... 29 0.93
TC218792 homologue to UP|O44991 (O44991) Lipid depleted protein ... 29 0.93
TC232974 weakly similar to GB|AAK54457.1|20384750|AY032998 DNA r... 29 0.93
TC216321 similar to UP|Q948Y7 (Q948Y7) VMP3 protein, partial (3%) 29 0.93
TC205210 similar to UP|Q9DWH4 (Q9DWH4) Pr4.1, partial (7%) 29 0.93
TC217047 homologue to UP|Q6TXM4 (Q6TXM4) Proton P-ATPase, partia... 29 0.93
AW317661 weakly similar to GP|18491291|gb| F14B2.25/F14B2.25 {Ar... 29 0.93
TC217708 similar to UP|O65896 (O65896) Cytidine deaminase 1 (At... 29 1.2
TC229074 weakly similar to GB|AAL69470.1|18491291|AY074654 F14B2... 28 1.6
TC205209 28 1.6
TC211828 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-pho... 28 2.1
>TC216013 homologue to UP|Q8L4X6 (Q8L4X6) WD-repeat protein GhTTG2, complete
Length = 1584
Score = 114 bits (286), Expect = 2e-26
Identities = 73/135 (54%), Positives = 80/135 (59%), Gaps = 7/135 (5%)
Frame = +1
Query: 33 NWSVRHDKKYHLAIASLLEQCPNCIEIV*LDDSNARSDPTRISPSSTRTHHQGHLHPR*G 92
NWSVR DKKY LAIASLLEQ PN +EIV LDDSN R PS + H
Sbjct: 163 NWSVRRDKKYRLAIASLLEQYPNRVEIVQLDDSNGE---IRSDPSLSFEH---------- 303
Query: 93 VPAPRPPRHLQRLPPRVA---DLRRR*NPTLR----RNPSVDLKSLLNGNKSSEYCGPLT 145
P PP +P + DL + LR SV+LKSLLNGNK+SEYCGPLT
Sbjct: 304 ---PYPPTKSIFIPDKDCHRPDLLATSSDFLRVWHISESSVELKSLLNGNKNSEYCGPLT 474
Query: 146 SFDWNEAEPCRIGTS 160
SFDWNEAEP RIGTS
Sbjct: 475 SFDWNEAEPRRIGTS 519
>TC216011 homologue to UP|Q8L4X6 (Q8L4X6) WD-repeat protein GhTTG2, partial
(71%)
Length = 852
Score = 71.6 bits (174), Expect(2) = 3e-17
Identities = 32/36 (88%), Positives = 35/36 (96%)
Frame = +3
Query: 125 SVDLKSLLNGNKSSEYCGPLTSFDWNEAEPCRIGTS 160
+V+LKSLLNGNK+SEYCGPLTSFDWNEAEP RIGTS
Sbjct: 66 AVELKSLLNGNKNSEYCGPLTSFDWNEAEPRRIGTS 173
Score = 32.7 bits (73), Expect(2) = 3e-17
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +2
Query: 94 PAPRPPRHLQRLPPRVADLRRR 115
P PR PRHL LPPR+A LR R
Sbjct: 2 PPPRSPRHLL*LPPRLAHLRIR 67
>TC216015 similar to UP|Q9M610 (Q9M610) Ttg1-like protein, partial (91%)
Length = 1151
Score = 62.0 bits (149), Expect = 1e-10
Identities = 50/167 (29%), Positives = 73/167 (42%), Gaps = 12/167 (7%)
Frame = +1
Query: 6 KTSQRTTRLSETSHRPVESKA---AKYQTKNWSVRHDKKYHLAIASLLEQCPNCIEIV*L 62
+T + +E SH E+ + Y S + LA+ S +E+ N ++I+
Sbjct: 13 ETRTKMENSTEESHLRSENSVTYESPYPIYGMSFSPSHPHRLALGSFIEEYNNRVDIL-- 186
Query: 63 DDSNARSDPTRISPSSTRTHHQGHLHPR*GVPAPRPPRHL-----QRLPPRVADLRRR*N 117
+ D ++P HP P PP L + P +DL
Sbjct: 187 ---SFHPDTLSVTP-----------HPSLSFDHPYPPTKLMFHPRKPSPSSSSDLLATSG 324
Query: 118 PTLR----RNPSVDLKSLLNGNKSSEYCGPLTSFDWNEAEPCRIGTS 160
LR R+ SVD SL N +K+SE+C PLTSFDWN+ +P RI TS
Sbjct: 325 DYLRLWEIRDNSVDAVSLFNNSKTSEFCAPLTSFDWNDIDPNRIATS 465
>TC216012 similar to UP|Q9M610 (Q9M610) Ttg1-like protein, partial (36%)
Length = 440
Score = 52.0 bits (123), Expect = 1e-07
Identities = 41/153 (26%), Positives = 69/153 (44%), Gaps = 4/153 (2%)
Frame = +1
Query: 5 RKTSQRTTRLSETSHRPVESKAAKYQTKNWSVRHDKKYHLAIASLLEQCPNCIEIV*LDD 64
R + +T+ S S + Y S + LA+ S +E+ N ++I+
Sbjct: 16 RTKMENSTQESHLRSENSVSYESPYPIYGMSFSPSHPHRLALGSFIEEYTNRVDIL---- 183
Query: 65 SNARSDPTRISPSSTRTHHQGHLHPR*GVPAPRPPRHLQRLPPRVADLRRR*NPTLR--- 121
+ D ++P+ + + HP P + H ++ P +DL LR
Sbjct: 184 -SFHPDTLSLTPNPSLSFD----HP---YPPTKLMFHPRKPPSSSSDLLATSGDYLRLWE 339
Query: 122 -RNPSVDLKSLLNGNKSSEYCGPLTSFDWNEAE 153
R+ SV+ SL N +K+SE+C PLTSFDWN+ +
Sbjct: 340 VRDNSVEAVSLFNNSKTSEFCAPLTSFDWNDID 438
>BG352594 similar to GP|22324809|gb| WD-repeat protein GhTTG4 {Gossypium
hirsutum}, partial (15%)
Length = 244
Score = 34.7 bits (78), Expect(2) = 1e-04
Identities = 19/40 (47%), Positives = 22/40 (54%), Gaps = 2/40 (5%)
Frame = +1
Query: 68 RSDPTRISP--SSTRTHHQGHLHPR*GVPAPRPPRHLQRL 105
R DP R P ++ HQ HLHPR G+P P HL RL
Sbjct: 103 RRDPIRSLPVLGASVPCHQMHLHPRQGLPPAGPACHLMRL 222
Score = 26.9 bits (58), Expect(2) = 1e-04
Identities = 14/21 (66%), Positives = 16/21 (75%), Gaps = 2/21 (9%)
Frame = +2
Query: 54 PNCIEIV*LDDSNA--RSDPT 72
PN +EIV LDDSN RSDP+
Sbjct: 65 PNRVEIVQLDDSNGEIRSDPS 127
>TC204966 similar to UP|HMD1_ARATH (P14891)
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
(HMG-CoA reductase 1) (HMGR1) , partial (30%)
Length = 753
Score = 32.0 bits (71), Expect = 0.14
Identities = 23/52 (44%), Positives = 26/52 (49%)
Frame = +2
Query: 82 HHQGHLHPR*GVPAPRPPRHLQRLPPRVADLRRR*NPTLRRNPSVDLKSLLN 133
H HL PR RP HL RLP R+ RRR N +L RN + L LN
Sbjct: 326 HRLLHLPPRLLRHRLRPVLHLTRLP-RLLGPRRRRNSSLPRNYQIALTGYLN 478
>TC219477 similar to UP|Q8LSC5 (Q8LSC5) Geranylgeranyl pyrophosphate
synthase, partial (77%)
Length = 1106
Score = 32.0 bits (71), Expect = 0.14
Identities = 36/106 (33%), Positives = 43/106 (39%), Gaps = 16/106 (15%)
Frame = +1
Query: 69 SDPTRISP-SSTRTHHQGHLHPR--------*GVPAP-RPPRHLQRLPP-----RVADLR 113
S P SP + R H+GH HPR V P RP H QR PP + DLR
Sbjct: 355 SAPCSASPHAXARRWHRGHRHPRRLRRRDDPHHVAHPRRPALHGQRRPPPWKADKPQDLR 534
Query: 114 R-R*NPTLRRNPSVDLKSLLNGNKSSEYCGPLTSFDWNEAEPCRIG 158
R R P RR P +++ G CG + D E R G
Sbjct: 535 RTRGRPRRRRAPRARVRA-RGGVDGGRVCGARGARDRGVGEMHRRG 669
>CA783163 similar to GP|8777474|dbj| contains similarity to rab3
GTPase-activating protein non-catalitic
subunit~gene_id:K15M2.5, partial (20%)
Length = 458
Score = 31.2 bits (69), Expect = 0.24
Identities = 22/45 (48%), Positives = 22/45 (48%), Gaps = 4/45 (8%)
Frame = +3
Query: 67 ARSDPTRISPSSTRTHHQGHLHPR*GVPAPRPPR----HLQRLPP 107
A DPTR P R H L PR PRPPR HL RLPP
Sbjct: 312 APQDPTRPLPHRCRVH----LRPR-VARLPRPPRHRRRHLLRLPP 431
>TC206672 weakly similar to UP|Q7UTL3 (Q7UTL3) 3-hydroxyisobutyrate
dehydrogenase , partial (76%)
Length = 1230
Score = 29.6 bits (65), Expect = 0.71
Identities = 22/55 (40%), Positives = 27/55 (49%), Gaps = 3/55 (5%)
Frame = +3
Query: 64 DSNARSDPTRISPSST-RTH-HQGHLHPR*GVPAPRPPRHLQRLPP-RVADLRRR 115
D + R P + P R H H+ HP G PRP L+ LPP R + LRRR
Sbjct: 99 DRHGRHGPVHVRPPHPCRLHPHRLQPHPLQGPAPPRPRGLLRPLPPRRRSPLRRR 263
>AW279280 similar to PIR|T04047|T0 isoleucine-tRNA ligase homolog F24G24.120
- Arabidopsis thaliana, partial (11%)
Length = 493
Score = 29.3 bits (64), Expect = 0.93
Identities = 22/51 (43%), Positives = 24/51 (46%), Gaps = 7/51 (13%)
Frame = +1
Query: 68 RSDPTRISPSSTRTHHQGHLHPR*GVPA-PRPPRH------LQRLPPRVAD 111
R P + P S R HHQGH H +P RPPRH L R P R D
Sbjct: 121 RHRPPSLRPHSRR-HHQGHRH---ALPLHDRPPRHPPIRLRLPRPPRRERD 261
>TC218792 homologue to UP|O44991 (O44991) Lipid depleted protein 6, partial
(4%)
Length = 756
Score = 29.3 bits (64), Expect = 0.93
Identities = 17/45 (37%), Positives = 21/45 (45%)
Frame = +1
Query: 71 PTRISPSSTRTHHQGHLHPR*GVPAPRPPRHLQRLPPRVADLRRR 115
P +PS++ H LHP P P R L+RLP RRR
Sbjct: 136 PLSPAPSNSHLHLHRVLHPPPSSPPPSRRRVLRRLPQSTILCRRR 270
>TC232974 weakly similar to GB|AAK54457.1|20384750|AY032998 DNA repair
protein XRCC3 {Arabidopsis thaliana;} , partial (40%)
Length = 909
Score = 29.3 bits (64), Expect = 0.93
Identities = 24/57 (42%), Positives = 26/57 (45%), Gaps = 9/57 (15%)
Frame = +1
Query: 66 NARSDPTR----ISPSSTRTHHQGHLHPR*GVPAPRPP-----RHLQRLPPRVADLR 113
+A PTR P S R+ HLHP VP P PP L RLPPR LR
Sbjct: 136 DAALPPTRPLRPAPPLSRRSLRLLHLHPH-RVPLPLPPPPPPLTRLPRLPPRPPLLR 303
>TC216321 similar to UP|Q948Y7 (Q948Y7) VMP3 protein, partial (3%)
Length = 661
Score = 29.3 bits (64), Expect = 0.93
Identities = 26/85 (30%), Positives = 37/85 (42%)
Frame = +2
Query: 69 SDPTRISPSSTRTHHQGHLHPR*GVPAPRPPRHLQRLPPRVADLRRR*NPTLRRNPSVDL 128
S PTR +PSS+ ++ R P+P PP L PR A P L RNP
Sbjct: 200 SPPTRPAPSSSSSNESRPWRERSTPPSPPPP----ALAPRSA------RPRLLRNP--PN 343
Query: 129 KSLLNGNKSSEYCGPLTSFDWNEAE 153
++L++ +S+ S W E
Sbjct: 344 RALISSPPNSKTPQRCLSCTWKSCE 418
>TC205210 similar to UP|Q9DWH4 (Q9DWH4) Pr4.1, partial (7%)
Length = 581
Score = 29.3 bits (64), Expect = 0.93
Identities = 22/60 (36%), Positives = 25/60 (41%), Gaps = 20/60 (33%)
Frame = +1
Query: 76 PSSTRTHHQGHLHPR*GVPAPRPPRH--------------------LQRLPPRVADLRRR 115
P R HQ LHPR PAP+PP H L RLP R++ L RR
Sbjct: 211 PPGDRLRHQV-LHPR---PAPQPPSHPTHRFEESRCRKAHEGADLRLLRLPSRLSQLHRR 378
>TC217047 homologue to UP|Q6TXM4 (Q6TXM4) Proton P-ATPase, partial (4%)
Length = 512
Score = 29.3 bits (64), Expect = 0.93
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = -2
Query: 137 SSEYCGPLTSF---DWNEAEPCRIGTSLTC 163
SS + PLT + EPC +GTSLTC
Sbjct: 154 SSPFTSPLTQHVLSXYXAPEPCNLGTSLTC 65
>AW317661 weakly similar to GP|18491291|gb| F14B2.25/F14B2.25 {Arabidopsis
thaliana}, partial (60%)
Length = 310
Score = 29.3 bits (64), Expect = 0.93
Identities = 19/57 (33%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +1
Query: 67 ARSDPTRISPSSTRTHHQGHLHPR*GVPAPRPPRH-LQRLPPRVADLRRR*NPTLRR 122
+R+ TR+ P HH+ + H R P+ PRH +QR P ++R +P +RR
Sbjct: 148 SRTGETRVPPHRNLRHHRPNQHSRGTGPSQPLPRHAVQRDPFH----KKRLHPRVRR 306
>TC217708 similar to UP|O65896 (O65896) Cytidine deaminase 1 (At2g19570) ,
partial (69%)
Length = 954
Score = 28.9 bits (63), Expect = 1.2
Identities = 21/54 (38%), Positives = 23/54 (41%), Gaps = 6/54 (11%)
Frame = +2
Query: 71 PTRISPSSTRTHHQGHLHPR*GVPAP------RPPRHLQRLPPRVADLRRR*NP 118
P + P T HHQ P G P RP R L +PPR RRR NP
Sbjct: 212 PPPLRPRRTVPHHQPL--PECGAPPGVPGRLRRPLRALPPIPPRTPRRRRRENP 367
>TC229074 weakly similar to GB|AAL69470.1|18491291|AY074654 F14B2.25/F14B2.25
{Arabidopsis thaliana;} , partial (83%)
Length = 707
Score = 28.5 bits (62), Expect = 1.6
Identities = 18/56 (32%), Positives = 26/56 (46%)
Frame = +3
Query: 67 ARSDPTRISPSSTRTHHQGHLHPR*GVPAPRPPRHLQRLPPRVADLRRR*NPTLRR 122
+R R+ P R HH+ + R VP+ PRH P V ++R +P RR
Sbjct: 249 SRRGEARVPPQRNRRHHRPNRPSRGTVPSRPLPRHAL---PLVPFHKKRRHPRFRR 407
>TC205209
Length = 704
Score = 28.5 bits (62), Expect = 1.6
Identities = 18/40 (45%), Positives = 21/40 (52%)
Frame = +1
Query: 76 PSSTRTHHQGHLHPR*GVPAPRPPRHLQRLPPRVADLRRR 115
P R HQ LHPR PAP+PP H R+ + RRR
Sbjct: 205 PPGDRLRHQV-LHPR---PAPQPPSHTTH---RLEEARRR 303
>TC211828 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-phosphate
isomerase (Phosphoriboisomerase) , partial (47%)
Length = 729
Score = 28.1 bits (61), Expect = 2.1
Identities = 22/54 (40%), Positives = 24/54 (43%), Gaps = 5/54 (9%)
Frame = +3
Query: 67 ARSDPTRISPSSTRTHHQG---HLH--PR*GVPAPRPPRHLQRLPPRVADLRRR 115
AR P R +P +T HLH PR G P PR P RLPP RR
Sbjct: 456 ARRRPHRRAPPPRKTQRHRRNPHLHQNPRPG-PLPRHPPLRSRLPPHRRSRHRR 614
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.328 0.139 0.451
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,191,282
Number of Sequences: 63676
Number of extensions: 156456
Number of successful extensions: 1586
Number of sequences better than 10.0: 92
Number of HSP's better than 10.0 without gapping: 1534
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1575
length of query: 168
length of database: 12,639,632
effective HSP length: 90
effective length of query: 78
effective length of database: 6,908,792
effective search space: 538885776
effective search space used: 538885776
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0150.5