BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0147a.4
(354 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP595172 polyprotein [Glycine max] 172 3e-43
BI425021 115 3e-26
CO982196 100 8e-22
CA820403 weakly similar to GP|13273463|gb| pol protein integrase... 70 2e-16
BQ299538 77 9e-15
TC211627 75 6e-14
BI424213 67 1e-11
AW830191 64 1e-10
TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 57 3e-09
BE660092 weakly similar to GP|9884624|dbj retroelement pol polyp... 50 1e-06
BI317638 weakly similar to GP|9294238|dbj| contains similarity t... 50 2e-06
TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, parti... 49 5e-06
TC233069 45 7e-05
TC234722 similar to UP|Q6WAY9 (Q6WAY9) Pol (Fragment), partial (... 42 4e-04
TC208972 33 0.15
BE801213 weakly similar to GP|6691193|gb| F7F22.17 {Arabidopsis ... 32 0.57
TC228057 28 6.3
TC215826 homologue to UP|Q40213 (Q40213) RAB7C, complete 28 6.3
BQ454192 28 8.2
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 172 bits (435), Expect = 3e-43
Identities = 84/165 (50%), Positives = 111/165 (66%), Gaps = 1/165 (0%)
Frame = +1
Query: 4 PTTVWENIYMDSIGGLPKSKGKETILVVVDRLTKFAHFLPLCHLFSASEVAAGFIQEIIR 63
P VWE++ MD I GLP S G I+VV+DRLTK+AHF+PL +++ VA F+ I++
Sbjct: 3415 PQQVWEDVAMDFITGLPNSFGLSVIMVVIDRLTKYAHFIPLKADYNSKVVAEAFMSHIVK 3594
Query: 64 LHGFPSTIVSDHDS-FPE*FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRL 122
LHG P +IVSD D F F + L + GT L SSAYHPQ+DGQ+EV+N+CLE YLR
Sbjct: 3595 LHGIPRSIVSDRDRVFTSTFWQHLFKLQGTTLAMSSAYHPQSDGQSEVLNKCLEMYLRCF 3774
Query: 123 IWTCPKNWSNCLAWAEFWFNTNYNTSRNMSPFNALSGQDPLSLLK 167
+ PK W L WAEFW+NT Y+ S M+PF AL G++P +L +
Sbjct: 3775 TYEHPKGWVKALPWAEFWYNTAYHMSLGMTPFRALYGREPPTLTR 3909
Score = 32.7 bits (73), Expect = 0.26
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = +1
Query: 218 VFLKLQPYFRRCLARRINEKLSPRFYGPLQ 247
V +KLQPY + R N+KLS R++GP +
Sbjct: 4072 VLVKLQPYRQHSAVLRKNQKLSMRYFGPFK 4161
>BI425021
Length = 426
Score = 115 bits (288), Expect = 3e-26
Identities = 64/136 (47%), Positives = 83/136 (60%), Gaps = 1/136 (0%)
Frame = -1
Query: 4 PTTVWENIYMDSIGGLPKSKGKETILVVVDRLTKFAHFLPLCHLFSASEVAAGFIQEIIR 63
P WE++ MD I GLP G TI VVV+R +K H L +A VA+ F+ +I+
Sbjct: 408 PQRPWEDLSMDFIVGLPPYHGHTTIFVVVNRFSKGIHLGTLPTSHTAHMVASLFLNIVIK 229
Query: 64 LHGFPSTIVSDHDS-FPE*FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRL 122
LHGFP +IVSD D F F + L + +GT L+ SSAYHPQTDGQTEV+NR +E YLR
Sbjct: 228 LHGFPRSIVSDRDPLFISHFWQDLFRLSGTVLRMSSAYHPQTDGQTEVLNRVIEQYLRAF 49
Query: 123 IWTCPKNWSNCLAWAE 138
+ P+N + W E
Sbjct: 48 VHGRPRNLGRFIPWVE 1
>CO982196
Length = 812
Score = 100 bits (250), Expect = 8e-22
Identities = 47/71 (66%), Positives = 59/71 (82%)
Frame = +1
Query: 4 PTTVWENIYMDSIGGLPKSKGKETILVVVDRLTKFAHFLPLCHLFSASEVAAGFIQEIIR 63
PT VW +I MD IGGLPK++GK+ ILVVVDRLTK+AHF L H ++A EVA FI+E++R
Sbjct: 571 PTKVWTDISMDFIGGLPKAQGKDNILVVVDRLTKYAHFFALSHPYTAKEVAELFIKELVR 750
Query: 64 LHGFPSTIVSD 74
LHGFP++IVSD
Sbjct: 751 LHGFPASIVSD 783
>CA820403 weakly similar to GP|13273463|gb| pol protein integrase region
{Ginkgo biloba}, partial (52%)
Length = 421
Score = 70.5 bits (171), Expect(3) = 2e-16
Identities = 39/78 (50%), Positives = 49/78 (62%), Gaps = 2/78 (2%)
Frame = -3
Query: 57 FIQEIIRLHGFPSTIVSDHDSF--PE*FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRC 114
FI+E ++LHG S+IVSD D *F L + GT+LKFS AYHPQ D T+VVNRC
Sbjct: 335 FIKEAVKLHGCSSSIVSDWDRLFLIS*FWTELFKMEGTKLKFSLAYHPQPDSHTKVVNRC 156
Query: 115 LEAYLRRLIWTCPKNWSN 132
+E L+ L + K W N
Sbjct: 155 IEMNLQCLTTSKRKQWPN 102
Score = 33.1 bits (74), Expect(3) = 2e-16
Identities = 14/34 (41%), Positives = 24/34 (70%)
Frame = -1
Query: 28 ILVVVDRLTKFAHFLPLCHLFSASEVAAGFIQEI 61
I+V+V R TK+AHF+ L H + A EV+ ++++
Sbjct: 421 IMVIVYRFTKYAHFVVLSHPYLAKEVSEVLLKKL 320
Score = 19.6 bits (39), Expect(3) = 2e-16
Identities = 5/10 (50%), Positives = 9/10 (90%)
Frame = -2
Query: 131 SNCLAWAEFW 140
+ CL+WAE++
Sbjct: 108 AKCLSWAEYF 79
>BQ299538
Length = 426
Score = 77.4 bits (189), Expect = 9e-15
Identities = 38/86 (44%), Positives = 54/86 (62%)
Frame = +3
Query: 77 SFPE*FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRLIWTCPKNWSNCLAW 136
+F E LE +++ GT LK S++YHP DGQT VN CLE +LR + PK L+W
Sbjct: 102 NFCEFILEGIVKLPGTYLKMSTSYHP*IDGQT--VNHCLETFLRCFVADQPKM*VQWLSW 275
Query: 137 AEFWFNTNYNTSRNMSPFNALSGQDP 162
AE+W+NTN++ S +PF + G+ P
Sbjct: 276 AEYWYNTNFHASTGTTPFEVVYGRKP 353
>TC211627
Length = 1034
Score = 74.7 bits (182), Expect = 6e-14
Identities = 34/65 (52%), Positives = 47/65 (72%)
Frame = +2
Query: 90 AGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRLIWTCPKNWSNCLAWAEFWFNTNYNTSR 149
+GT+L+FS+AYHPQTDGQTEV+NR LE YLR + P++W L+ AE +NT+ ++
Sbjct: 41 SGTKLRFSTAYHPQTDGQTEVINRILEQYLRAFVHDHPQHWFKFLSLAE*CYNTSVHSGI 220
Query: 150 NMSPF 154
SPF
Sbjct: 221 GFSPF 235
>BI424213
Length = 426
Score = 67.0 bits (162), Expect = 1e-11
Identities = 36/85 (42%), Positives = 48/85 (56%)
Frame = +1
Query: 82 FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRLIWTCPKNWSNCLAWAEFWF 141
F + L GT+L FS+ HPQTDGQT+VVNR L LR L+ K+W L EF +
Sbjct: 10 FWKTLWAKLGTKLLFSTTCHPQTDGQTKVVNRSLSTLLRALLKGNHKSWDEYLPHVEFAY 189
Query: 142 NTNYNTSRNMSPFNALSGQDPLSLL 166
N + + SPF + G +PL+ L
Sbjct: 190 NRGVHRTTKQSPFEVVYGFNPLTPL 264
>AW830191
Length = 372
Score = 63.5 bits (153), Expect = 1e-10
Identities = 29/50 (58%), Positives = 33/50 (66%)
Frame = +3
Query: 119 LRRLIWTCPKNWSNCLAWAEFWFNTNYNTSRNMSPFNALSGQDPLSLLKG 168
LR L T P+ W L+WAEFWFNTNYN S ++PF L G DP LLKG
Sbjct: 6 LRCLTGTKPQQWPKRLSWAEFWFNTNYNNSLKLTPFKVLYGCDPPHLLKG 155
Score = 32.0 bits (71), Expect = 0.44
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = +3
Query: 218 VFLKLQPYFRRCLARRINEK 237
V+LKLQPY +R LAR+ NEK
Sbjct: 312 VYLKLQPYRQRSLARKTNEK 371
>TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 730
Score = 57.4 bits (137), Expect(2) = 3e-09
Identities = 48/137 (35%), Positives = 70/137 (51%), Gaps = 7/137 (5%)
Frame = +1
Query: 218 VFLKLQPYFRRCLARRINEKLSPRFYGPLQG*ER*LTSHSYLPEVRYTWDF-----MFPL 272
VFLK+QPY RR LA+RINEKLSPRFY P Q + + + Y D + P+
Sbjct: 154 VFLKMQPYRRRSLAKRINEKLSPRFYAPFQ-------VFNKVGTIAYKLDLPSHIKIHPV 312
Query: 273 LRRWWELISRFSSCL--KCCLRITSS*FSLQMFWTLGKQLRGILRC*FNGRIFLLVRLVG 330
L+ + S L C LRI + +F + R + *F+G+I L V++VG
Sbjct: 313 FH--VSLLKKASPNLYHLCYLRIGNYKLIQILF*IVVNYNRAT*KF*FSGKISLPVKIVG 486
Query: 331 SRLRGC*KLYQPLTLRT 347
+ + ++ +TLRT
Sbjct: 487 NLWLNYKRFFRFITLRT 537
Score = 21.6 bits (44), Expect(2) = 3e-09
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = +2
Query: 176 KR*IS*YVVEMSCWLI*GKIC*HDAFFC 203
K+ I+* ++ M WL+* KIC* C
Sbjct: 17 KKSIN**LLGMDYWLL*EKIC*SLKISC 100
>BE660092 weakly similar to GP|9884624|dbj retroelement pol polyprotein-like
{Arabidopsis thaliana}, partial (13%)
Length = 378
Score = 50.4 bits (119), Expect = 1e-06
Identities = 25/78 (32%), Positives = 42/78 (53%)
Frame = -3
Query: 83 LEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRLIWTCPKNWSNCLAWAEFWFN 142
+ L++ G + S+ YHPQT+GQ E+ NR ++ L +++ K+WS L A +
Sbjct: 376 MHALLKKYGVVHRVSTPYHPQTNGQAEISNREIKRILEKIVQPSRKDWSTRLDDALWAHR 197
Query: 143 TNYNTSRNMSPFNALSGQ 160
T Y MSP+ + G+
Sbjct: 196 TAYKAPIGMSPYRVVFGK 143
>BI317638 weakly similar to GP|9294238|dbj| contains similarity to reverse
transcriptase~gene_id:K11J14.5 {Arabidopsis thaliana},
partial (5%)
Length = 420
Score = 49.7 bits (117), Expect = 2e-06
Identities = 29/73 (39%), Positives = 38/73 (51%)
Frame = -2
Query: 4 PTTVWENIYMDSIGGLPKSKGKETILVVVDRLTKFAHFLPLCHLFSASEVAAGFIQEIIR 63
P WE++ +D I GL ILVVVD +K H L +A VA FI + +
Sbjct: 257 PHRPWEDLSLDFITGLLPYHVHTAILVVVDHFSKGIHLGMLPSSHTAHTVACLFIDSVAK 78
Query: 64 LHGFPSTIVSDHD 76
LHG P ++VSD D
Sbjct: 77 LHGLPRSLVSDCD 39
>TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, partial (7%)
Length = 810
Score = 48.5 bits (114), Expect = 5e-06
Identities = 44/141 (31%), Positives = 69/141 (48%), Gaps = 4/141 (2%)
Frame = +3
Query: 218 VFLKLQPYFRRCLARRINEKLSPRFYGPLQG*ER--*LTSHSYLPEVRYTWDFMFPLLRR 275
V+LKLQPY + LA++ NEKLSPRFYGP Q ++ + LP R L +
Sbjct: 60 VYLKLQPYRLKSLAKKRNEKLSPRFYGPYQIKKQIGLVAFELDLPPARKIHPVFHASLLK 239
Query: 276 WWELISRFSSCLKCCLR--ITSS*FSLQMFWTLGKQLRGILRC*FNGRIFLLVRLVGSRL 333
+ L L ++S F L+ ++ L +L+ FN +IF +++ +G+
Sbjct: 240 KAVAATANPQPLPLMLSEDLSSEFFQLKSKLSI-TILMVLLKFSFNWKIFRILKPLGNPW 416
Query: 334 RGC*KLYQPLTLRTT*NSWGQ 354
+ + TLRT * WG+
Sbjct: 417 KSSRNSFPHFTLRTR*LFWGE 479
>TC233069
Length = 881
Score = 44.7 bits (104), Expect = 7e-05
Identities = 45/149 (30%), Positives = 64/149 (42%), Gaps = 7/149 (4%)
Frame = +2
Query: 4 PTTVWENIYMDSIGGLPKSKGKETILVVVDRLTKFAHFLPL-CHLFSASEVAAGFIQEII 62
P VW++I MD + LP S GK+ I V+VD TK++HFL L HL GF+ E
Sbjct: 326 P*QVWKDISMDFVTHLPPS*GKKMIWVIVDCWTKYSHFLSLPAHLSYIQP*G*GFVFE-- 499
Query: 63 RLHGFPSTIVSDHDSFPE*------FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLE 116
G + D + P * +V Q G + +P+ G +E
Sbjct: 500 --GGSIDIDIQDKHTCPV*T*SRCLLAQVKHQQKGRE*GLRKTSYPKVKGL-----NAME 658
Query: 117 AYLRRLIWTCPKNWSNCLAWAEFWFNTNY 145
++L L CP N L F++NY
Sbjct: 659 SHLSCLRTLCPARPENSLT----*FSSNY 733
>TC234722 similar to UP|Q6WAY9 (Q6WAY9) Pol (Fragment), partial (32%)
Length = 482
Score = 42.0 bits (97), Expect = 4e-04
Identities = 20/61 (32%), Positives = 35/61 (56%)
Frame = -3
Query: 100 YHPQTDGQTEVVNRCLEAYLRRLIWTCPKNWSNCLAWAEFWFNTNYNTSRNMSPFNALSG 159
YHPQT+GQ EV N+ ++ L ++ + K+W+ L A + + + T +SPF + G
Sbjct: 234 YHPQTNGQAEVSNKEIKRVLENIVVSSRKDWALKLDDAFWAYRIAFKTPIGLSPFQLVYG 55
Query: 160 Q 160
+
Sbjct: 54 K 52
>TC208972
Length = 1190
Score = 33.5 bits (75), Expect = 0.15
Identities = 28/87 (32%), Positives = 41/87 (46%), Gaps = 5/87 (5%)
Frame = -3
Query: 38 FAHFLPLCHLFSASEVAAGFIQEIIRLHGFPSTIVSDH---DSFPE*FLEVLIQGAGTQL 94
+AH + L F+ + VA+ F EI L+G P I+ H L + A
Sbjct: 1047 YAHLIALPTTFTTTRVASRFFVEIAHLNGIP-*IIDRH*L*QGLH*CVLVGAFRVARNFS 871
Query: 95 KFSSAYH--PQTDGQTEVVNRCLEAYL 119
+FS+ YH Q+DG E +RCL + L
Sbjct: 870 EFSTTYHLEIQSDGSHESFHRCLPSML 790
>BE801213 weakly similar to GP|6691193|gb| F7F22.17 {Arabidopsis thaliana},
partial (3%)
Length = 416
Score = 31.6 bits (70), Expect = 0.57
Identities = 20/67 (29%), Positives = 33/67 (48%), Gaps = 1/67 (1%)
Frame = +3
Query: 59 QEIIRLHGFPSTIVSDHDS-FPE*FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEA 117
+ I G P ++SD S F L +++ + K ++YHPQT+GQ +V N E
Sbjct: 36 KNIFSRFGMPRILISDGGSHFYYSQLNKVLKHDSVRHKVETSYHPQTNGQAKVSNIHKEN 215
Query: 118 YLRRLIW 124
+ +W
Sbjct: 216 S*EKKMW 236
>TC228057
Length = 774
Score = 28.1 bits (61), Expect = 6.3
Identities = 15/67 (22%), Positives = 27/67 (39%)
Frame = +3
Query: 3 PPTTVWENIYMDSIGGLPKSKGKETILVVVDRLTKFAHFLPLCHLFSASEVAAGFIQEII 62
PP TVW T++ D + HF+ CH+ ++A G +++
Sbjct: 210 PPHTVW------------------TVVRCFDNPQAYKHFIKSCHVKEGFQLAVGSTRDVH 335
Query: 63 RLHGFPS 69
+ G P+
Sbjct: 336 VISGLPA 356
>TC215826 homologue to UP|Q40213 (Q40213) RAB7C, complete
Length = 1000
Score = 28.1 bits (61), Expect = 6.3
Identities = 10/31 (32%), Positives = 15/31 (48%)
Frame = +3
Query: 114 CLEAYLRRLIWTCPKNWSNCLAWAEFWFNTN 144
C+ + L++ NWSN + W WF N
Sbjct: 774 CVRRHHCTLVFAQETNWSNAIRWVLVWFCDN 866
>BQ454192
Length = 175
Score = 27.7 bits (60), Expect = 8.2
Identities = 9/29 (31%), Positives = 19/29 (65%)
Frame = -1
Query: 257 SYLPEVRYTWDFMFPLLRRWWELISRFSS 285
+YL ++ TW P+++ W++I R++S
Sbjct: 112 NYLMNLQVTWAIPPPIIKMQWDVIGRYTS 26
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.349 0.154 0.574
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,240,815
Number of Sequences: 63676
Number of extensions: 342606
Number of successful extensions: 3276
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 3218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3265
length of query: 354
length of database: 12,639,632
effective HSP length: 98
effective length of query: 256
effective length of database: 6,399,384
effective search space: 1638242304
effective search space used: 1638242304
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0147a.4