BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0142.1
(142 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW164266 38 0.001
TC216046 weakly similar to UP|Q70Z43 (Q70Z43) Translation initia... 36 0.005
TC231215 similar to UP|Q6L4C3 (Q6L4C3) Expressed protein, partia... 35 0.016
TC228274 similar to UP|Q8VYK5 (Q8VYK5) At2g36680/F13K3.8, partia... 34 0.020
BE554860 34 0.027
TC230827 weakly similar to GB|AAO42927.1|28416793|BT004681 At5g2... 32 0.078
TC216669 similar to GB|BAC06968.1|22093674|AP003815 kinase-like ... 32 0.10
BE661588 32 0.10
TC221026 similar to GB|AAM47347.1|21360463|AY113039 AT5g38560/MB... 32 0.13
TC231486 similar to UP|Q9LNR9 (Q9LNR9) F1L3.5, partial (28%) 31 0.17
TC215145 similar to GB|CAC81059.1|17939851|ATH271469 mitochondri... 31 0.23
TC221692 UP|Q9FEC5 (Q9FEC5) Glycinin subunit G7, complete 30 0.29
TC215144 similar to UP|ATPQ_ARATH (Q9FT52) ATP synthase D chain,... 30 0.38
TC212142 similar to UP|Q8L869 (Q8L869) RanGAP1 interacting prote... 30 0.38
TC216950 29 0.66
BQ297369 29 0.86
TC215147 similar to GB|CAC81059.1|17939851|ATH271469 mitochondri... 29 0.86
BE660842 similar to GP|11139266|gb PRLI-interacting factor K {Ar... 29 0.86
TC208480 29 0.86
TC208939 UP|Q39830 (Q39830) BiP isoform A, complete 28 1.1
>AW164266
Length = 435
Score = 38.1 bits (87), Expect = 0.001
Identities = 27/73 (36%), Positives = 40/73 (53%)
Frame = +3
Query: 29 FEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLK 88
F E+L L I PE T+ I E R+ ++E L L L+ F A+ E+Q+ LL
Sbjct: 30 FTEEL-LQIELPESTSLIEEKFRSNMDEILEENLTFWLK-FSAYYSEIQKFETTIKDLLT 203
Query: 89 EKERLEEKLKTAE 101
E +LEE+ K++E
Sbjct: 204 ELSKLEEREKSSE 242
>TC216046 weakly similar to UP|Q70Z43 (Q70Z43) Translation initiation factor
3, partial (11%)
Length = 909
Score = 36.2 bits (82), Expect = 0.005
Identities = 23/80 (28%), Positives = 40/80 (49%)
Frame = +2
Query: 58 LRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQ 117
LR E E + L + +E R LK +E ++KL+ AEE +R++ + + +
Sbjct: 50 LRREREERIXRILQSRRQEREKMRKLKYYLKLEEERQQKLREAEEARKREDAERKKKEEE 229
Query: 118 HALERLEKDRSEQRQECRRM 137
L +LE+ +QRQ R +
Sbjct: 230 ERLRKLEEIAEKQRQREREL 289
>TC231215 similar to UP|Q6L4C3 (Q6L4C3) Expressed protein, partial (97%)
Length = 920
Score = 34.7 bits (78), Expect = 0.016
Identities = 29/106 (27%), Positives = 50/106 (46%), Gaps = 26/106 (24%)
Frame = +1
Query: 60 PELERDLREFLAFVEEV-------------------QELSRLELKLLKEKERLEEKLKTA 100
P L++ ++F +FV EV QEL+ E+ LLK K +E ++
Sbjct: 181 PPLKKLQKKFTSFVSEVDKDEDNNYNECDKLARAFLQELTTFEIPLLKSKAIVEANVREK 360
Query: 101 EEILERDELKDR-----LSNIQHALERLEKDRSEQR--QECRRMRR 139
E E E +R S+I+ ++LE+ + E+R +EC +R+
Sbjct: 361 ENFNELKEEMNRQILLAQSDIEDLKKQLEESKVERRHKEECEAIRK 498
>TC228274 similar to UP|Q8VYK5 (Q8VYK5) At2g36680/F13K3.8, partial (55%)
Length = 1129
Score = 34.3 bits (77), Expect = 0.020
Identities = 27/85 (31%), Positives = 46/85 (53%), Gaps = 5/85 (5%)
Frame = +3
Query: 59 RPELERDLREFLAFVEEVQELSRLEL-KLLKEKERLEEKLKTAEEILERDELKDRL--SN 115
RP E A + +++ S EL KLL +K+ ++ L + +++ ++ LKD L N
Sbjct: 258 RPSSHVPPSEAAAVIAVLKDKSVDELRKLLSDKDAYQQFLHSLDQVKIQNNLKDELCKEN 437
Query: 116 IQHALERLEKDR--SEQRQECRRMR 138
+Q A E L+K+ E R +CR +R
Sbjct: 438 LQLAEENLQKEPRIMELRNQCRIIR 512
>BE554860
Length = 411
Score = 33.9 bits (76), Expect = 0.027
Identities = 23/69 (33%), Positives = 40/69 (57%), Gaps = 2/69 (2%)
Frame = +1
Query: 74 EEVQELSRLELKLLKEKERLEEK-LKTAEEILERDELKDRLSNIQHALERLEKD-RSEQR 131
E+ Q+L RLE+ L++ E LEE+ +K EEI ++ +L + +++ L +D + +R
Sbjct: 154 EQRQKLRRLEVALIEYGESLEERGIKNLEEIEKKVQLHRKRLQVEYGLSDSGEDGQGNRR 333
Query: 132 QECRRMRRD 140
RR R D
Sbjct: 334 TSERRDRHD 360
>TC230827 weakly similar to GB|AAO42927.1|28416793|BT004681 At5g24660
{Arabidopsis thaliana;} , partial (41%)
Length = 570
Score = 32.3 bits (72), Expect = 0.078
Identities = 25/71 (35%), Positives = 37/71 (51%), Gaps = 5/71 (7%)
Frame = +2
Query: 75 EVQELSRLELKLLKEKERLEEKLKTAEEILE--RDELK---DRLSNIQHALERLEKDRSE 129
E + S LEL+L K E LEE+L ++E E R +L+ DRL+ + A ERL +
Sbjct: 71 ECETTSSLELQLKKRNEELEEELSQSKEREEHVRRQLRAALDRLTVAEEAEERLCAQLGD 250
Query: 130 QRQECRRMRRD 140
E + R+
Sbjct: 251 LEAEALQQARE 283
>TC216669 similar to GB|BAC06968.1|22093674|AP003815 kinase-like protein
{Oryza sativa (japonica cultivar-group);} , partial
(16%)
Length = 1336
Score = 32.0 bits (71), Expect = 0.10
Identities = 20/63 (31%), Positives = 32/63 (50%)
Frame = +1
Query: 77 QELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRSEQRQECRR 136
QE RLE +E+ R+E ++KTAE R+ + + +R EK+R R +
Sbjct: 727 QEKERLERIQREERARIEAQIKTAEAAA-------RMRAEEESRQRREKEREASRAAIEK 885
Query: 137 MRR 139
M+R
Sbjct: 886 MKR 894
>BE661588
Length = 697
Score = 32.0 bits (71), Expect = 0.10
Identities = 24/80 (30%), Positives = 41/80 (51%)
Frame = +1
Query: 51 RTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELK 110
RTL+E++L + + D+ E+ E ++ LK L +RL E+LK LER + +
Sbjct: 40 RTLVEQELE-KAQEDIPEYKKQAEAAEQEKGQVLKELDSTKRLIEELKLN---LERAQTE 207
Query: 111 DRLSNIQHALERLEKDRSEQ 130
+R + L +L + EQ
Sbjct: 208 ERQARQDSELAKLRVEEMEQ 267
>TC221026 similar to GB|AAM47347.1|21360463|AY113039 AT5g38560/MBB18_10
{Arabidopsis thaliana;} , partial (3%)
Length = 617
Score = 31.6 bits (70), Expect = 0.13
Identities = 22/77 (28%), Positives = 43/77 (55%), Gaps = 3/77 (3%)
Frame = -3
Query: 66 LREFLAFVEEVQELS-RLELKLLKEKERLEEKLKTAEEILERDELKDRLSNI--QHALER 122
+R F F E +++ R E + E+E LEE +T EE++ ++R + + +E+
Sbjct: 420 MRAFTNFEEGDEDVV*RRESHVETEEEELEEGFRTQEEVVGSKVKEEREG*LLFRWMMEK 241
Query: 123 LEKDRSEQRQECRRMRR 139
+ K + E+R+ +RMR+
Sbjct: 240 MPKAKVERRKRRKRMRK 190
>TC231486 similar to UP|Q9LNR9 (Q9LNR9) F1L3.5, partial (28%)
Length = 1024
Score = 31.2 bits (69), Expect = 0.17
Identities = 27/97 (27%), Positives = 51/97 (51%), Gaps = 7/97 (7%)
Frame = +2
Query: 51 RTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERD--- 107
+ +L ED P ++ +F EE+ +L + KLL++K + EK + AE L+R
Sbjct: 119 KNILRED--PSSSEEIADFTP--EEIGKLKKELTKLLRQKPNIVEK-EIAELPLDRFLNC 283
Query: 108 ----ELKDRLSNIQHALERLEKDRSEQRQECRRMRRD 140
E+ R+SN+ + + +KD+ E+++E R +
Sbjct: 284 PSSLEVDRRISNVLGS-DSEDKDKDEEKEEEREKEEE 391
>TC215145 similar to GB|CAC81059.1|17939851|ATH271469 mitochondrial F0 ATP
synthase D chain {Arabidopsis thaliana;} , partial (62%)
Length = 795
Score = 30.8 bits (68), Expect = 0.23
Identities = 25/63 (39%), Positives = 33/63 (51%)
Frame = +1
Query: 78 ELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRSEQRQECRRM 137
EL E K LKE ERLE +EI+E ELK +LS + A E EK +++ +
Sbjct: 100 ELKEAEEKSLKESERLE------KEIVEVQELKKKLSTMT-ADEYFEKHPELRKKFDDEI 258
Query: 138 RRD 140
R D
Sbjct: 259 RND 267
>TC221692 UP|Q9FEC5 (Q9FEC5) Glycinin subunit G7, complete
Length = 1674
Score = 30.4 bits (67), Expect = 0.29
Identities = 23/82 (28%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = +2
Query: 58 LRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNI- 116
+RP LE ++RE EV+E + +KER ++ + EE+++ E++ R +I
Sbjct: 746 IRPPLEHEVRE-----AEVEEKPKTREHCECQKERKHKEGEGEEEVVQEKEIRKRKHHIG 910
Query: 117 QHALERLEKDRSEQRQECRRMR 138
+H +D+ E+ Q R R
Sbjct: 911 EHEGCGECEDKEEEEQSRSRER 976
>TC215144 similar to UP|ATPQ_ARATH (Q9FT52) ATP synthase D chain,
mitochondrial , complete
Length = 843
Score = 30.0 bits (66), Expect = 0.38
Identities = 25/63 (39%), Positives = 32/63 (50%)
Frame = +3
Query: 78 ELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRSEQRQECRRM 137
EL E K LKE ERLE +EI E ELK +LS + A E EK +++ +
Sbjct: 393 ELKEAEEKSLKESERLE------KEIAEVQELKKKLSTMT-ADEYFEKHPELRKKFDDEI 551
Query: 138 RRD 140
R D
Sbjct: 552 RND 560
>TC212142 similar to UP|Q8L869 (Q8L869) RanGAP1 interacting protein, partial
(16%)
Length = 701
Score = 30.0 bits (66), Expect = 0.38
Identities = 33/89 (37%), Positives = 44/89 (49%), Gaps = 6/89 (6%)
Frame = +3
Query: 44 TTIAELIRT--LLEEDLRPEL---ERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLK 98
T +A L+R +LE L L E + + + QE+ R LKE E L EKL
Sbjct: 213 TKVASLMRKFGILEGQLAVALASREAAEKSLASAFKSRQEMERKLADTLKEMELLREKLV 392
Query: 99 TAEEILERDELKDRLSNIQHALE-RLEKD 126
AE L ++E + LSN+ HA RLE D
Sbjct: 393 NAE--LAQEE-ANNLSNMVHADNVRLEHD 470
>TC216950
Length = 873
Score = 29.3 bits (64), Expect = 0.66
Identities = 26/74 (35%), Positives = 33/74 (44%), Gaps = 3/74 (4%)
Frame = +2
Query: 55 EEDLRPE---LERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKD 111
EE R E LER L E VEE Q LE ++ R EE+ K EE+ + E
Sbjct: 419 EEQARKEKEDLERMLEENRRKVEEAQRREALE-----QQRREEERYKELEEMQRQKEEAM 583
Query: 112 RLSNIQHALERLEK 125
R + ERL +
Sbjct: 584 RKKKHEEEQERLNQ 625
>BQ297369
Length = 419
Score = 28.9 bits (63), Expect = 0.86
Identities = 22/82 (26%), Positives = 42/82 (50%), Gaps = 2/82 (2%)
Frame = +3
Query: 56 EDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILE--RDELKDRL 113
+D EL R + E ++ + SR+ + + L+ +++TA + LE R L DRL
Sbjct: 69 KDFSTELIRKIEEAISGTSNNSKSSRVTARF-RSISSLKYQIQTALDQLEASRKMLLDRL 245
Query: 114 SNIQHALERLEKDRSEQRQECR 135
I +E+ +++ E+ +CR
Sbjct: 246 LEIDQTMEKPKEEDIERVGKCR 311
>TC215147 similar to GB|CAC81059.1|17939851|ATH271469 mitochondrial F0 ATP
synthase D chain {Arabidopsis thaliana;} , partial (81%)
Length = 443
Score = 28.9 bits (63), Expect = 0.86
Identities = 24/63 (38%), Positives = 32/63 (50%)
Frame = +1
Query: 78 ELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRSEQRQECRRM 137
E+ E K LKE ERLE +EI E ELK +LS + A E EK +++ +
Sbjct: 184 EVKEAEEKSLKESERLE------KEIAEVQELKKKLSTMT-ADEYFEKHPELRKKFDDEI 342
Query: 138 RRD 140
R D
Sbjct: 343 RND 351
>BE660842 similar to GP|11139266|gb PRLI-interacting factor K {Arabidopsis
thaliana}, partial (26%)
Length = 854
Score = 28.9 bits (63), Expect = 0.86
Identities = 21/57 (36%), Positives = 27/57 (46%)
Frame = +3
Query: 74 EEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRSEQ 130
E+ + R LKLLKEK+ EE K E I E R I A +L+ D+ Q
Sbjct: 90 EQRERKERARLKLLKEKKAKEEAQKQKEAI----EAVQRSRRIDAAQAQLKADQQMQ 248
>TC208480
Length = 1576
Score = 28.9 bits (63), Expect = 0.86
Identities = 25/102 (24%), Positives = 49/102 (47%), Gaps = 6/102 (5%)
Frame = +3
Query: 23 ELRKKAFEEDLFLNIR-----HPEGTTTIAELIRTLLEEDLRPELERDLREFLAFVEEVQ 77
+++K+A ED N++ H + T E I+ L EE+ ++ D
Sbjct: 69 DVQKEAISEDTIRNLKEKNDVHVQKETLSQETIKKLKEEN-EVHIQEDF----------- 212
Query: 78 ELSRLELKLLKEK-ERLEEKLKTAEEILERDELKDRLSNIQH 118
LS+ +K LKE+ ++L +K+ + EE++ + + L +H
Sbjct: 213 -LSKETIKNLKEENDKLLQKVVSLEEVINNLQTDNELQTQKH 335
>TC208939 UP|Q39830 (Q39830) BiP isoform A, complete
Length = 2452
Score = 28.5 bits (62), Expect = 1.1
Identities = 23/99 (23%), Positives = 44/99 (44%)
Frame = +3
Query: 5 EQFSVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELER 64
E+F+ +KK+ I A L + + + + + + LE D + ++E
Sbjct: 1713 EEFAEEDKKVKERIDARNSLETYVY---------NMKNQVSDKDKLADKLESDEKEKVET 1865
Query: 65 DLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEI 103
++E L ++++ Q + EKE EEKLK E +
Sbjct: 1866 AVKEALEWLDDNQSV---------EKEEYEEKLKEVEAV 1955
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.135 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,736,192
Number of Sequences: 63676
Number of extensions: 29944
Number of successful extensions: 309
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 307
length of query: 142
length of database: 12,639,632
effective HSP length: 88
effective length of query: 54
effective length of database: 7,036,144
effective search space: 379951776
effective search space used: 379951776
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0142.1