BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0135.12
(76 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC214462 homologue to gb|AF036501.1| Lithophragma trifoliata lar... 116 2e-27
TC214600 homologue to gb|AF036489.1|AF036489 Ephedra distachya l... 67 1e-12
TC214602 similar to PIR|T47605|T47605 RING finger-like protein -... 60 3e-10
TC214539 44 2e-05
TC212489 homologue to gb|AF223570.1|AF223570 Spathidium amphorif... 34 4e-05
BQ630627 33 0.021
TC218491 homologue to UP|Q8SAG1 (Q8SAG1) ATP sulfurylase, partia... 28 1.1
TC207468 27 2.5
TC225163 emb|X02559.1|MIOBRN26 Oenothera berteriana mitochondria... 25 5.6
BM891964 25 7.3
TC206816 similar to UP|Q6ZH45 (Q6ZH45) Sialyltransferase-like pr... 25 9.5
>TC214462 homologue to gb|AF036501.1| Lithophragma trifoliata large subunit 26S
ribosomal RNA gene, partial sequence, partial (63%)
Length = 2159
Score = 116 bits (291), Expect = 2e-27
Identities = 56/71 (78%), Positives = 59/71 (82%), Gaps = 2/71 (2%)
Frame = -3
Query: 8 SPNSELEALSHNPTHGSFAPLTSQTSVMTNCANQRFLFN*VE--LRHFHQ*GKTNMSHDG 65
SP+S+LEA SHNPTHGSFAPL Q S MTNCANQRFL *VE LRH HQ*GKTN+SHDG
Sbjct: 1899 SPDSDLEAFSHNPTHGSFAPLAFQPSAMTNCANQRFLSY*VELLLRHCHQ*GKTNLSHDG 1720
Query: 66 LIPTHVPYWWV 76
L P HVPYWWV
Sbjct: 1719 LNPAHVPYWWV 1687
>TC214600 homologue to gb|AF036489.1|AF036489 Ephedra distachya large subunit
26S ribosomal RNA gene, partial sequence, partial (14%)
Length = 677
Score = 67.4 bits (163), Expect = 1e-12
Identities = 35/68 (51%), Positives = 43/68 (62%), Gaps = 3/68 (4%)
Frame = -1
Query: 12 ELEALSHNPTHGSFAPLTSQTSVMTNCANQRFLFN*VE---LRHFHQ*GKTNMSHDGLIP 68
++E SHNPT G +A L Q + +T NQRFL V + HQ*GKTN+SHDG+ P
Sbjct: 539 DVEGASHNPTDGGWAALAVQLTAVTKGGNQRFL*EGVGGLVRQRTHQ*GKTNLSHDGVNP 360
Query: 69 THVPYWWV 76
HVPY WV
Sbjct: 359 AHVPY*WV 336
>TC214602 similar to PIR|T47605|T47605 RING finger-like protein - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (10%)
Length = 858
Score = 59.7 bits (143), Expect = 3e-10
Identities = 27/37 (72%), Positives = 29/37 (77%)
Frame = -3
Query: 8 SPNSELEALSHNPTHGSFAPLTSQTSVMTNCANQRFL 44
SP+S+LEA SHNPTHG F PL Q MTNCANQRFL
Sbjct: 112 SPDSDLEAFSHNPTHGVFPPLGFQPRAMTNCANQRFL 2
>TC214539
Length = 407
Score = 43.5 bits (101), Expect = 2e-05
Identities = 18/24 (75%), Positives = 21/24 (87%)
Frame = -1
Query: 8 SPNSELEALSHNPTHGSFAPLTSQ 31
SP+S+LEA SHNPTHGSFAPL +
Sbjct: 77 SPDSDLEAFSHNPTHGSFAPLAGR 6
>TC212489 homologue to gb|AF223570.1|AF223570 Spathidium amphoriforme
internal transcribed spacer 1, 5.8S ribosomal RNA gene,
internal transcribed spacer 2, and 28S ribosomal RNA
gene, complete sequence, partial (4%)
Length = 263
Score = 34.3 bits (77), Expect(2) = 4e-05
Identities = 14/19 (73%), Positives = 16/19 (83%)
Frame = -2
Query: 55 Q*GKTNMSHDGLIPTHVPY 73
+* KTN+SHDGL P HVPY
Sbjct: 76 R*DKTNLSHDGLNPAHVPY 20
Score = 27.7 bits (60), Expect(2) = 4e-05
Identities = 17/41 (41%), Positives = 21/41 (50%)
Frame = -3
Query: 10 NSELEALSHNPTHGSFAPLTSQTSVMTNCANQRFLFN*VEL 50
N++LE SHN G F L ++ TN RFL *V L
Sbjct: 225 NTDLETFSHNSAAGRFNALLFPITLYTN*LI*RFLSY*VRL 103
>BQ630627
Length = 296
Score = 33.5 bits (75), Expect = 0.021
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = -1
Query: 8 SPNSELEALSHNPTHG 23
SP+S+LEA SHNPTHG
Sbjct: 53 SPDSDLEAFSHNPTHG 6
>TC218491 homologue to UP|Q8SAG1 (Q8SAG1) ATP sulfurylase, partial (36%)
Length = 651
Score = 27.7 bits (60), Expect = 1.1
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = +3
Query: 7 YSPNSELEALSHNPTHGSFAPLTSQTSVMTNCANQRF 43
Y +S+ + L HNP+ S A +QTS ++ ++ F
Sbjct: 93 YKAHSQRDQLQHNPSMASMATFFTQTSFPSHSLSKTF 203
>TC207468
Length = 638
Score = 26.6 bits (57), Expect = 2.5
Identities = 10/33 (30%), Positives = 18/33 (54%)
Frame = +2
Query: 5 HRYSPNSELEALSHNPTHGSFAPLTSQTSVMTN 37
H+Y P L+A ++P H +P+ T+ T+
Sbjct: 86 HKYIPRFHLQACKNHPPHHPSSPVFCSTTTTTS 184
>TC225163 emb|X02559.1|MIOBRN26 Oenothera berteriana mitochondrial gene for 26S
ribosomal RNA, partial (83%)
Length = 3184
Score = 25.4 bits (54), Expect = 5.6
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -3
Query: 59 TNMSHDGLIPTHVP 72
T +SHD L PTHVP
Sbjct: 2912 TELSHDVLNPTHVP 2871
>BM891964
Length = 423
Score = 25.0 bits (53), Expect = 7.3
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -2
Query: 18 HNPTHGSFAPLTSQTSVMTNCANQR 42
H+P H A L+ Q V +CA++R
Sbjct: 383 HDPQHQQAASLSPQPQVQHSCASRR 309
>TC206816 similar to UP|Q6ZH45 (Q6ZH45) Sialyltransferase-like protein,
partial (7%)
Length = 550
Score = 24.6 bits (52), Expect = 9.5
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +3
Query: 6 RYSPNSELEALSHNPTHGSFAPLTSQTSVMTNC 38
RY + + L H+P HGS + + +V+ C
Sbjct: 168 RYVKRTTMYPLEHSPGHGSLCTVPTD*TVLRFC 266
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.332 0.139 0.486
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,239,205
Number of Sequences: 63676
Number of extensions: 53205
Number of successful extensions: 452
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 450
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 450
length of query: 76
length of database: 12,639,632
effective HSP length: 52
effective length of query: 24
effective length of database: 9,328,480
effective search space: 223883520
effective search space used: 223883520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0135.12