BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0134.24
(366 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 321 3e-88
CO982349 229 1e-60
AW757033 152 3e-37
BI972659 139 2e-33
AI496436 112 9e-26
TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {A... 96 3e-20
TC212055 87 1e-17
CA783280 weakly similar to PIR|T01871|T01 RNA-directed DNA polym... 79 2e-15
CF922212 71 9e-13
CD417230 weakly similar to GP|2244915|emb| reverse transcriptase... 69 3e-12
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 66 3e-11
BM891241 62 5e-10
BU762692 57 2e-08
BI698930 54 8e-08
BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase ... 52 4e-07
BM891637 52 4e-07
TC234877 50 2e-06
BI784748 47 2e-05
TC213164 44 1e-04
CF920672 37 0.011
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 321 bits (823), Expect = 3e-88
Identities = 156/303 (51%), Positives = 215/303 (70%)
Frame = +1
Query: 29 LDSVLVANEVVDEARRQGKECMIFKVDYKKAYDSVRSDFLYYMMRRLGFCEKWIGWIKAC 88
L S L+ANEV+DEA+R K C++FKVDY+KAYDSV +FL YMM R+ F KWI WI+ C
Sbjct: 1 LHSALIANEVIDEAKRSNKSCLVFKVDYEKAYDSVSWNFLMYMMWRMDFSPKWIKWIEEC 180
Query: 89 IQSATVSVLVNGSPTEESVMQRGLRQGDPLAPFLFLVVAEWLSGLMRQARRLNLFKGYKV 148
++SA++SVLVNGSPT E + QRGLRQGDPL PFLF +VAE L+GLMR+A NL+K Y V
Sbjct: 181 VKSASISVLVNGSPTAEFLPQRGLRQGDPLTPFLFNIVAEGLNGLMRRAVEENLYKPYLV 360
Query: 149 GMDGMEISLLQFLDDTLFIGEASTQNVLVLKGMLRYFELLSGLKVNFSKNRFPGISLGEG 208
G +G+ IS+LQ+ DDT+F GEA+ +NV +K +LR FEL+S L++NF+K+ F + +
Sbjct: 361 GANGVPISILQYADDTIFFGEAAKENVEAIKVILRSFELVSNLRINFAKSCFGVFGVTDQ 540
Query: 209 TTQCFARMLNCRVMKEPFTYLGIFVGANPRRRSMWEPLVCKMKKRLQSWKQKMLSFGGKV 268
Q A L+C ++ PF YLGI +GAN RR MW+ ++ K +++L WK + +SFGG+V
Sbjct: 541 WKQEAANYLHCSLLAFPFIYLGIPIGANSRRCHMWDSIIRKCERKLSKWK*RHISFGGRV 720
Query: 269 CLIKSVLSSIPLFYLSFFKFPRGIAKECKRIQMDFLWGREVGAGKICWAKWSTVCKIKEE 328
LIKSVL+SIP+++ SFF+ P+ + + +I FLWG KI W +W +C KE
Sbjct: 721 TLIKSVLTSIPIYFFSFFRVPQSVVDKLVKI*RTFLWGGGPDQKKISWIRWEKLCLPKER 900
Query: 329 EGL 331
G+
Sbjct: 901 GGI 909
>CO982349
Length = 795
Score = 229 bits (584), Expect = 1e-60
Identities = 111/240 (46%), Positives = 161/240 (66%)
Frame = +3
Query: 84 WIKACIQSATVSVLVNGSPTEESVMQRGLRQGDPLAPFLFLVVAEWLSGLMRQARRLNLF 143
WI+ C+ SA+VS+LVN SP E QRGLRQGDPLAP LF +VAE L+GLMR+A F
Sbjct: 69 WIRGCLHSASVSILVNESPINEFSPQRGLRQGDPLAPLLFNIVAEGLTGLMREAVDRKRF 248
Query: 144 KGYKVGMDGMEISLLQFLDDTLFIGEASTQNVLVLKGMLRYFELLSGLKVNFSKNRFPGI 203
+ VG + +S+LQ+ DDT+F EA+ +N+ V+K +LR FEL SGLK+NF+++RF I
Sbjct: 249 NSFLVGKNKEPVSILQYADDTIFFEEATMENMKVIKTILRCFELASGLKINFAESRFGAI 428
Query: 204 SLGEGTTQCFARMLNCRVMKEPFTYLGIFVGANPRRRSMWEPLVCKMKKRLQSWKQKMLS 263
+ + A LNC ++ PF+YLGI + ANPR R + +P++ K + +L WKQ+ +S
Sbjct: 429 WKPDHWCKEAAEFLNCSMLSMPFSYLGIPIEANPRCREI*DPIIRKCETKLARWKQRHIS 608
Query: 264 FGGKVCLIKSVLSSIPLFYLSFFKFPRGIAKECKRIQMDFLWGREVGAGKICWAKWSTVC 323
GG+V LI ++L+++P+++ SFF+ P + ++ IQ FLWG +I W KW TVC
Sbjct: 609 LGGRVTLINAILTALPIYFFSFFRAPSKVVQKLVNIQRKFLWGGGSXQRRIAWVKWETVC 788
>AW757033
Length = 441
Score = 152 bits (383), Expect = 3e-37
Identities = 75/145 (51%), Positives = 102/145 (69%)
Frame = -2
Query: 103 TEESVMQRGLRQGDPLAPFLFLVVAEWLSGLMRQARRLNLFKGYKVGMDGMEISLLQFLD 162
T E RGLRQGDPLAP LF + AE L+GLMR+A N +K + VG +S+LQ+ D
Sbjct: 440 TREFSPHRGLRQGDPLAPLLFNIAAEGLTGLMREAVARNHYKSFAVGKYKKPVSILQYAD 261
Query: 163 DTLFIGEASTQNVLVLKGMLRYFELLSGLKVNFSKNRFPGISLGEGTTQCFARMLNCRVM 222
DT+F GEA+ +NV V+K +LR FEL SGLK+NF+K+RF IS+ + + A +NC ++
Sbjct: 260 DTIFFGEATMENVRVIKTILRGFELASGLKINFAKSRFGAISVPD*WRKEAAEFMNCSLL 81
Query: 223 KEPFTYLGIFVGANPRRRSMWEPLV 247
PF+YLGI +GANPRRR W+P++
Sbjct: 80 SLPFSYLGIPIGANPRRRETWDPII 6
>BI972659
Length = 453
Score = 139 bits (351), Expect = 2e-33
Identities = 65/150 (43%), Positives = 96/150 (63%)
Frame = +1
Query: 167 IGEASTQNVLVLKGMLRYFELLSGLKVNFSKNRFPGISLGEGTTQCFARMLNCRVMKEPF 226
+GEAS N++VLK MLR FE++SGL++N++K++F + A++LNCR + PF
Sbjct: 1 VGEASWDNIIVLKSMLRGFEMVSGLRINYAKSQFGIVGFQPNWAHDAAQLLNCRQLDIPF 180
Query: 227 TYLGIFVGANPRRRSMWEPLVCKMKKRLQSWKQKMLSFGGKVCLIKSVLSSIPLFYLSFF 286
YLG+ + R +WEPL+ K K +L W QK LS GG+V LIKSVL+++P++ LSFF
Sbjct: 181 HYLGMPIAVKASSRMVWEPLINKFKAKLSKWNQKYLSMGGRVTLIKSVLNALPIYLLSFF 360
Query: 287 KFPRGIAKECKRIQMDFLWGREVGAGKICW 316
K P+ I + +Q F+WG +I W
Sbjct: 361 KIPQRIVDKLVSLQRTFMWGGNQHHNRISW 450
>AI496436
Length = 414
Score = 112 bits (280), Expect(2) = 9e-26
Identities = 55/109 (50%), Positives = 77/109 (70%)
Frame = +1
Query: 181 MLRYFELLSGLKVNFSKNRFPGISLGEGTTQCFARMLNCRVMKEPFTYLGIFVGANPRRR 240
+LR FEL SGLK+NF+K++F I + + A LN V+ PF YLGI +GAN R
Sbjct: 4 ILRCFELSSGLKINFAKSQFGTICKSDIWRKEAADYLNSSVLSMPFVYLGIPIGANSRHS 183
Query: 241 SMWEPLVCKMKKRLQSWKQKMLSFGGKVCLIKSVLSSIPLFYLSFFKFP 289
+WEP+V K +++L SWKQK +SFGG+V LI SVL+++P++ LSFF+ P
Sbjct: 184 DVWEPVVRKCERKLASWKQKYVSFGGRVTLINSVLTALPIYLLSFFRIP 330
Score = 22.3 bits (46), Expect(2) = 9e-26
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = +2
Query: 299 IQMDFLWGREVGAGKICW 316
IQ FLWG ++ KI W
Sbjct: 359 IQRRFLWGGDLE*RKIAW 412
>TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {Arabidopsis
thaliana;} , partial (18%)
Length = 759
Score = 95.9 bits (237), Expect = 3e-20
Identities = 43/98 (43%), Positives = 59/98 (59%)
Frame = +2
Query: 265 GGKVCLIKSVLSSIPLFYLSFFKFPRGIAKECKRIQMDFLWGREVGAGKICWAKWSTVCK 324
GG+V LIKSVLS++P+ LSFFK P+ I + +Q FLWG +I W KW+ +C
Sbjct: 5 GGRVTLIKSVLSALPIXXLSFFKIPQRIVDKLVTLQRQFLWGGTQHHNRIPWVKWADICN 184
Query: 325 IKEEEGLGVKDLERFNRALMSKWRWRNLGEEDRLWGEV 362
K + GLG+KDL FN AL +W W ++LW +
Sbjct: 185 PKIDGGLGIKDLSNFNAALRGRWIWGLASNHNQLWARL 298
>TC212055
Length = 776
Score = 87.0 bits (214), Expect = 1e-17
Identities = 46/113 (40%), Positives = 71/113 (62%), Gaps = 8/113 (7%)
Frame = +1
Query: 122 LFLVVAEWLSGLMRQARRLNLFKGYKVGMDGMEISLLQFLDDTLFIGEASTQNVLVLKGM 181
LF +VAE L+GLMR+A +L+ VG + + +++LQ+ DDT+F+GEA+ QNV+ +K M
Sbjct: 1 LFNIVAEGLTGLMREALDKSLYSSLMVGKNKIPVNILQYADDTIFLGEATMQNVMTIKSM 180
Query: 182 LRYFELLSGLKVNFSKNRFPGI--------SLGEGTTQCFARMLNCRVMKEPF 226
LR FEL SGLK++F+K+ F I G T C +++ R + + F
Sbjct: 181 LRVFELASGLKIHFAKSSFGAIGKPDHWQKEAGLNTFNCRNLIVHIRQLDQAF 339
>CA783280 weakly similar to PIR|T01871|T01 RNA-directed DNA polymerase
homolog T24M8.8 - Arabidopsis thaliana, partial (4%)
Length = 456
Score = 79.3 bits (194), Expect = 2e-15
Identities = 36/83 (43%), Positives = 46/83 (55%)
Frame = +2
Query: 284 SFFKFPRGIAKECKRIQMDFLWGREVGAGKICWAKWSTVCKIKEEEGLGVKDLERFNRAL 343
SFF P I + + +Q FLWG E + KI W W TVC K + GLG+KDL FN L
Sbjct: 5 SFFSLP*KIIAKLESLQRRFLWGGEADSRKIAWVNWKTVCLPKAKGGLGIKDLRTFNTTL 184
Query: 344 MSKWRWRNLGEEDRLWGEVLSSR 366
+ KWRW + W +VL S+
Sbjct: 185 LGKWRWDLFYIQQEPWAKVLQSK 253
>CF922212
Length = 445
Score = 70.9 bits (172), Expect = 9e-13
Identities = 33/78 (42%), Positives = 44/78 (56%)
Frame = -2
Query: 289 PRGIAKECKRIQMDFLWGREVGAGKICWAKWSTVCKIKEEEGLGVKDLERFNRALMSKWR 348
P + + R+Q FLWG +G KI W W +VC KE+EGLG +DL +FN AL+ K R
Sbjct: 438 PNKVLDKLVRLQQWFLWGGGLGQKKIAWVNWDSVCLPKEKEGLGNRDLRKFNYALLGKRR 259
Query: 349 WRNLGEEDRLWGEVLSSR 366
W + L VL S+
Sbjct: 258 WNLFHHQGELGARVLDSK 205
>CD417230 weakly similar to GP|2244915|emb| reverse transcriptase like
protein {Arabidopsis thaliana}, partial (7%)
Length = 688
Score = 69.3 bits (168), Expect = 3e-12
Identities = 43/122 (35%), Positives = 64/122 (52%), Gaps = 3/122 (2%)
Frame = -3
Query: 88 CIQSATVSVLVNGSPTEESVMQRGLRQGDPLAPFLFLVVAEWLSGLMRQARRLNLFKGYK 147
C+ + T+S+L NG + G+RQ DP+AP+LF++ E LS L+ L + +
Sbjct: 668 CMSTTTMSLLWNGEVLDHFSPNIGVRQRDPIAPYLFVLCLERLSHLINLVMNKKL*RSIQ 489
Query: 148 VGMDGMEISLLQFLDDTLFIGEASTQNVLVLKGMLRYFELLSGLKVNFSKNR---FPGIS 204
V G+ IS L F+DD + EA+ V V+ L F SG KVN K + FP +
Sbjct: 488 VNRGGLLISHLAFVDDLILFVEANMDQVDVINLTLELFSDSSGAKVNVDKTKSFLFPKML 309
Query: 205 LG 206
+G
Sbjct: 308 IG 303
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 65.9 bits (159), Expect = 3e-11
Identities = 29/74 (39%), Positives = 46/74 (61%)
Frame = -3
Query: 50 MIFKVDYKKAYDSVRSDFLYYMMRRLGFCEKWIGWIKACIQSATVSVLVNGSPTEESVMQ 109
+ K+D KA+D++ DFL ++ + G+ + WI+ + SA +S+ VNG P Q
Sbjct: 765 LALKIDIVKAFDTLDRDFLISVLNKFGYSHTFCNWIRVILLSAKLSISVNGEPVGFFSCQ 586
Query: 110 RGLRQGDPLAPFLF 123
RG+RQGDPL+P L+
Sbjct: 585 RGVRQGDPLSPSLY 544
>BM891241
Length = 407
Score = 61.6 bits (148), Expect = 5e-10
Identities = 23/54 (42%), Positives = 35/54 (64%)
Frame = -2
Query: 313 KICWAKWSTVCKIKEEEGLGVKDLERFNRALMSKWRWRNLGEEDRLWGEVLSSR 366
KI W KW VC K + GLG+KD+ +FN AL+ KW W ++ +LW +++S+
Sbjct: 385 KIPWVKWEVVCLPKTKGGLGIKDISKFNIALLGKWIWALASDQQQLWARIINSK 224
>BU762692
Length = 423
Score = 56.6 bits (135), Expect = 2e-08
Identities = 28/59 (47%), Positives = 38/59 (63%)
Frame = -3
Query: 181 MLRYFELLSGLKVNFSKNRFPGISLGEGTTQCFARMLNCRVMKEPFTYLGIFVGANPRR 239
+LR FEL+SGLK+NF+K+ F + + A LNC ++ PF Y GI +GANPRR
Sbjct: 181 ILRTFELVSGLKINFAKSCFGAFGMTDRWKTEAASCLNCSLLAIPFVYQGIPIGANPRR 5
>BI698930
Length = 384
Score = 54.3 bits (129), Expect = 8e-08
Identities = 30/71 (42%), Positives = 39/71 (54%)
Frame = +3
Query: 252 KRLQSWKQKMLSFGGKVCLIKSVLSSIPLFYLSFFKFPRGIAKECKRIQMDFLWGREVGA 311
K L WK ++LSF G+ CLI S+LSS+PLF SFFK P + I +F+ G +
Sbjct: 75 K*LALWKHEVLSFAGRTCLINSMLSSLPLFSFSFFKVPLNVGMRFISI*RNFV-GEWRES 251
Query: 312 GKICWAKWSTV 322
I W W V
Sbjct: 252 *MIPWVNWDKV 284
>BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase (EC
2.7.7.49) (clone AmLi2) - garden snapdragon (fragment),
partial (22%)
Length = 111
Score = 52.0 bits (123), Expect = 4e-07
Identities = 23/36 (63%), Positives = 27/36 (74%)
Frame = +1
Query: 86 KACIQSATVSVLVNGSPTEESVMQRGLRQGDPLAPF 121
K C+ SA++S+LVNGSPTEE RGLRQG P PF
Sbjct: 1 KECLSSASISILVNGSPTEEFKPXRGLRQGGPFGPF 108
>BM891637
Length = 427
Score = 52.0 bits (123), Expect = 4e-07
Identities = 20/54 (37%), Positives = 30/54 (55%)
Frame = -1
Query: 313 KICWAKWSTVCKIKEEEGLGVKDLERFNRALMSKWRWRNLGEEDRLWGEVLSSR 366
KI W W C + GLG+KD++ N AL+ KW+W + +LW +L S+
Sbjct: 415 KIAWISWRQCCASGDVGGLGIKDIKILNNALLIKWKWLMFHQPHQLWNRILISK 254
>TC234877
Length = 534
Score = 50.1 bits (118), Expect = 2e-06
Identities = 30/96 (31%), Positives = 46/96 (47%), Gaps = 2/96 (2%)
Frame = +1
Query: 225 PFTYLGIFVGANPRRRSMWEPLVCKMKKRLQSWKQKMLSFGGKVCLIKSVLSSIPLFYLS 284
PF YL + + R P+ ++K LQSWK +LSF G V LI S++ + L
Sbjct: 40 PFIYLSVPLFQEKPRPVHLRPIADRIKAELQSWKGNLLSFMGCVQLIISIIDGMLL*SFH 219
Query: 285 FFKFPRGIAKECKRIQMDFLWGREVGAGK--ICWAK 318
+ +P+ + E + +F+W R K CW K
Sbjct: 220 LYSWPKALLIEMQTWTRNFIWTRGYFKEKTGYCWLK 327
>BI784748
Length = 259
Score = 46.6 bits (109), Expect = 2e-05
Identities = 21/32 (65%), Positives = 27/32 (83%)
Frame = +2
Query: 16 DESQSAFIGGRHMLDSVLVANEVVDEARRQGK 47
D+ QSAFI GRHML S+++ANEV+DEA+R K
Sbjct: 5 DKRQSAFIQGRHMLHSMMIANEVIDEAKRGQK 100
>TC213164
Length = 446
Score = 43.9 bits (102), Expect = 1e-04
Identities = 20/40 (50%), Positives = 30/40 (75%)
Frame = +3
Query: 1 KLLSKRMGRVLGEVIDESQSAFIGGRHMLDSVLVANEVVD 40
KLLS R +VL +IDE Q+ F+ GRH L +V++ANE+++
Sbjct: 303 KLLSGRHKKVLPTIIDERQTVFMEGRHTLHNVVIANEIME 422
>CF920672
Length = 792
Score = 37.4 bits (85), Expect = 0.011
Identities = 19/52 (36%), Positives = 27/52 (51%)
Frame = -2
Query: 289 PRGIAKECKRIQMDFLWGREVGAGKICWAKWSTVCKIKEEEGLGVKDLERFN 340
P +A++ +IQ F WG + KI W KW C + E+E L VK +N
Sbjct: 791 PNRVAEKLTQIQRRFXWGGGLDQKKIAWVKWD--C*LMEQEPLRVKYPRLYN 642
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.140 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,157,562
Number of Sequences: 63676
Number of extensions: 280695
Number of successful extensions: 1948
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 1932
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1947
length of query: 366
length of database: 12,639,632
effective HSP length: 99
effective length of query: 267
effective length of database: 6,335,708
effective search space: 1691634036
effective search space used: 1691634036
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0134.24