BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0134.16
(212 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC206759 weakly similar to GB|AAF40464.1|7211993|F13M7 ESTs gb|T... 265 1e-71
TC220010 weakly similar to GB|AAF40464.1|7211993|F13M7 ESTs gb|T... 108 1e-24
BE058300 56 1e-08
BI497720 53 1e-07
TC229047 weakly similar to GB|AAN40705.2|28933849|AF226656 perox... 33 0.12
TC215627 similar to UP|Q9LNJ1 (Q9LNJ1) F6F3.12 protein, partial ... 30 0.62
TC229046 similar to UP|Q9SHX4 (Q9SHX4) F1E22.18, partial (36%) 29 1.8
BI321604 28 3.1
TC216488 weakly similar to UP|CFI_PHAVU (P14298) Chalcone--flavo... 27 6.9
TC218397 27 6.9
TC216347 homologue to UP|O81413 (O81413) Ferric leghemoglobin re... 27 6.9
BI786305 weakly similar to GP|18700073|gb AT5g12080/MXC9_3 {Arab... 27 8.9
>TC206759 weakly similar to GB|AAF40464.1|7211993|F13M7 ESTs gb|T76367 and
gb|AA404955 come from this gene. {Arabidopsis thaliana;}
, partial (21%)
Length = 2131
Score = 265 bits (676), Expect = 1e-71
Identities = 135/199 (67%), Positives = 162/199 (80%), Gaps = 1/199 (0%)
Frame = +3
Query: 1 VKGMQVGLTVNLKNQEGTLKLSLLDYGCYVGDLSIKLDGGTAWLYQLLVDAFGGNIASSV 60
V +QVGLTVNL+NQEGTLKL LLD GC+V DLSIKL GG AWLYQ+LVDAF GNIAS+V
Sbjct: 693 VNDLQVGLTVNLRNQEGTLKLILLDSGCHVRDLSIKLHGGAAWLYQVLVDAFAGNIASAV 872
Query: 61 EEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNVSFVGNPVLSNSSIAIAINGLFTER 120
EEA+S+KINEGI+ LD LQSLPK IPLD+T+ALNVSFV NPVLS+S+I + INGLFT R
Sbjct: 873 EEAISKKINEGISTLDLLLQSLPKTIPLDETAALNVSFVDNPVLSDSAIELEINGLFTGR 1052
Query: 121 SEVVESEGYKKGFKISSACG-GLPNMIKVSLHEYVIQSASLVYFNAGKMQLIIDELPDQV 179
+EV+ + Y+ +S++CG P MI +SLHE V +S SLVYF A MQ I+DELPDQ
Sbjct: 1053NEVLVPQAYRF*SDLSASCGDSSPKMITISLHESVFKSGSLVYFTADSMQWIVDELPDQA 1232
Query: 180 ILNTAECRFIVPQLYKQYP 198
+LNTAE RF++PQLYK+YP
Sbjct: 1233LLNTAEWRFLIPQLYKKYP 1289
>TC220010 weakly similar to GB|AAF40464.1|7211993|F13M7 ESTs gb|T76367 and
gb|AA404955 come from this gene. {Arabidopsis thaliana;}
, partial (38%)
Length = 815
Score = 108 bits (271), Expect = 1e-24
Identities = 54/104 (51%), Positives = 77/104 (73%)
Frame = +3
Query: 1 VKGMQVGLTVNLKNQEGTLKLSLLDYGCYVGDLSIKLDGGTAWLYQLLVDAFGGNIASSV 60
V+GM+VGLT+ L+NQEG+LKL L D G V D+SIKLDGG +WLYQ +VDAF I S+V
Sbjct: 504 VEGMEVGLTLGLENQEGSLKLKLKDCGSNVKDISIKLDGGASWLYQGIVDAFEDKIGSTV 683
Query: 61 EEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNVSFVGNPVL 104
E A+++K+ +GI+ LD + + K P+D +++NV+F GN V+
Sbjct: 684 ENAIAKKLTKGISSLDSYFEKXSKGGPVDDHASMNVTF-GNDVI 812
>BE058300
Length = 262
Score = 56.2 bits (134), Expect = 1e-08
Identities = 25/42 (59%), Positives = 34/42 (80%)
Frame = +1
Query: 157 SASLVYFNAGKMQLIIDELPDQVILNTAECRFIVPQLYKQYP 198
SA+ +Y++A M I+D++PDQ +LNTA RFIVPQLYK+YP
Sbjct: 7 SATALYYDAKFMHWIVDQIPDQSLLNTAGWRFIVPQLYKKYP 132
>BI497720
Length = 424
Score = 52.8 bits (125), Expect = 1e-07
Identities = 25/39 (64%), Positives = 30/39 (76%)
Frame = +2
Query: 8 LTVNLKNQEGTLKLSLLDYGCYVGDLSIKLDGGTAWLYQ 46
LT+ L+NQE +LKL L D G V D+SIKLDGG +WLYQ
Sbjct: 5 LTLGLENQEESLKLKLKDCGSNVKDISIKLDGGASWLYQ 121
>TC229047 weakly similar to GB|AAN40705.2|28933849|AF226656 peroxisomal
N1-acetyl-spermine/spermidine oxidase {Mus musculus;} ,
partial (7%)
Length = 706
Score = 32.7 bits (73), Expect = 0.12
Identities = 39/139 (28%), Positives = 60/139 (43%)
Frame = +1
Query: 60 VEEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSALNVSFVGNPVLSNSSIAIAINGLFTE 119
V +AV + GI K + ++ PK +P K A+ +GN + IA+ + +F
Sbjct: 232 VADAVIVTVPIGILKAN-LIEFSPK-LPHWKAEAIKDIGMGN----ENKIALRFDAVFWP 393
Query: 120 RSEVVESEGYKKGFKISSACGGLPNMIKVSLHEYVIQSASLVYFNAGKMQLIIDELPDQV 179
EV+ S ACG N+ K + H LVY AGK +++L D+
Sbjct: 394 NVEVLGIVA-----PTSYACGYFLNLHKATGHPI------LVYMAAGKFAYDLEKLSDES 540
Query: 180 ILNTAECRFIVPQLYKQYP 198
N F + QL K +P
Sbjct: 541 AAN-----FAMQQLKKMFP 582
>TC215627 similar to UP|Q9LNJ1 (Q9LNJ1) F6F3.12 protein, partial (4%)
Length = 697
Score = 30.4 bits (67), Expect = 0.62
Identities = 21/73 (28%), Positives = 37/73 (49%), Gaps = 2/73 (2%)
Frame = +1
Query: 19 LKLSLLDYGCYVGDLSIKLDGGTAWLYQLL--VDAFGGNIASSVEEAVSEKINEGIAKLD 76
LK + +G Y D ++ + GT + +++ V ++ S+ + V+EK+ G D
Sbjct: 85 LKKRTISHGSYTDDHTVNITQGTKFGRKVVKAVTYRVKSVPSTSKPCVNEKLENG----D 252
Query: 77 KFLQSLPKQIPLD 89
K L SLP+ P D
Sbjct: 253 KLLSSLPEPDPTD 291
>TC229046 similar to UP|Q9SHX4 (Q9SHX4) F1E22.18, partial (36%)
Length = 893
Score = 28.9 bits (63), Expect = 1.8
Identities = 21/63 (33%), Positives = 29/63 (45%)
Frame = +1
Query: 136 SSACGGLPNMIKVSLHEYVIQSASLVYFNAGKMQLIIDELPDQVILNTAECRFIVPQLYK 195
S ACG N+ K + H LVY AGK +++L D+ N F + QL K
Sbjct: 253 SYACGYFLNLHKATGHPI------LVYMAAGKFAYDLEKLSDESAAN-----FAMQQLKK 399
Query: 196 QYP 198
+P
Sbjct: 400 MFP 408
>BI321604
Length = 386
Score = 28.1 bits (61), Expect = 3.1
Identities = 23/86 (26%), Positives = 37/86 (42%), Gaps = 11/86 (12%)
Frame = +2
Query: 57 ASSVEEAVSEKINEGIAKLDKFLQSLPKQIPLDKTSAL-----------NVSFVGNPVLS 105
AS + + E N+GI L L L KQI ++ + VS+V +PV +
Sbjct: 95 ASKPRKQLGEPANDGIGSLSLELNQLKKQIQAERIVCIKEKNSEQ*EEATVSYVRSPVRN 274
Query: 106 NSSIAIAINGLFTERSEVVESEGYKK 131
+ G FT+R + +S + K
Sbjct: 275 IN------KGFFTDRGK*EKSNSFFK 334
>TC216488 weakly similar to UP|CFI_PHAVU (P14298) Chalcone--flavonone
isomerase (Chalcone isomerase) , partial (95%)
Length = 913
Score = 26.9 bits (58), Expect = 6.9
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +1
Query: 28 CYVGDLSIKLDGGTAWLYQLL 48
C+ G SI G T+WLYQ+L
Sbjct: 91 CHCGVPSIPRTGDTSWLYQIL 153
>TC218397
Length = 1398
Score = 26.9 bits (58), Expect = 6.9
Identities = 16/62 (25%), Positives = 29/62 (45%), Gaps = 8/62 (12%)
Frame = -1
Query: 136 SSACGGLPNMIKVSLHEYVIQSASLV--------YFNAGKMQLIIDELPDQVILNTAECR 187
S+ G + +S+++ + SL +F + L+IDE PD +L + + R
Sbjct: 1122 STTTSGRTGFLSISINDVSDTAPSLKEEDLNRTSFFPEASVMLLIDEFPDVSLLPSPDVR 943
Query: 188 FI 189
FI
Sbjct: 942 FI 937
>TC216347 homologue to UP|O81413 (O81413) Ferric leghemoglobin reductase-2
precursor , complete
Length = 1897
Score = 26.9 bits (58), Expect = 6.9
Identities = 33/151 (21%), Positives = 62/151 (40%), Gaps = 23/151 (15%)
Frame = +3
Query: 1 VKGMQVGLTVNLKNQEGTLKLSLLDYGCYVGDLSIKLDGGTAWLYQLLVDAFG--GNIAS 58
+K Q+GL + GTL + L+ GC + ++ +Y AF G S
Sbjct: 240 IKAAQLGLKTTCIEKRGTLGGTCLNVGCIPSKALLH----SSHMYHEAKHAFANHGVKFS 407
Query: 59 SVE---EAVSEKINEGIAKLDKFLQSL-----------------PKQIPLDKTSALNVSF 98
SVE A+ + ++ ++ L K ++ L P ++ +D T N
Sbjct: 408 SVEVDLPAMMAQKDKAVSNLTKGIEGLFKKNKVNYVKGYGKFVSPSEVSVDTTEGGNTVV 587
Query: 99 VG-NPVLSNSSIAIAINGLFTERSEVVESEG 128
G + +++ S ++ G+ + +VV S G
Sbjct: 588 KGKHIIIATGSDVKSLPGITIDEKKVVSSTG 680
>BI786305 weakly similar to GP|18700073|gb AT5g12080/MXC9_3 {Arabidopsis
thaliana}, partial (15%)
Length = 421
Score = 26.6 bits (57), Expect = 8.9
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = +1
Query: 35 IKLDGGTAWLYQLLVDAFGGNIASSVEEAVSEKINEG 71
+K + +AW ++LVDA + S++ A+ E +EG
Sbjct: 298 MKQEKVSAWTMKVLVDAMTTSGLSTISSALDESFDEG 408
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.138 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,892,075
Number of Sequences: 63676
Number of extensions: 89626
Number of successful extensions: 391
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 389
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 390
length of query: 212
length of database: 12,639,632
effective HSP length: 93
effective length of query: 119
effective length of database: 6,717,764
effective search space: 799413916
effective search space used: 799413916
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0134.16