TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0132.5
         (458 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC210419 similar to UP|NFS1_ARATH (O49543) Cysteine desulfurase,...   180  2e-77
BE024008 similar to SP|O49543|NFS1_ Cysteine desulfurase  mitoch...   141  7e-34
TC218074 similar to UP|Q93WX6 (Q93WX6) NIFS-like protein CpNifsp...    90  2e-18
BE210295 homologue to SP|O49543|NFS1_ Cysteine desulfurase  mito...    78  7e-15
BE474515                                                               42  4e-04
TC217615 similar to UP|SGPL_ARATH (Q9C509) Sphingosine-1-phospha...    42  8e-04
TC214997 similar to UP|Q9SS31 (Q9SS31) Calmodulin-like protein (...    30  1.7
TC216400 homologue to UP|Q6NPD6 (Q6NPD6) At2g22125, partial (69%)      29  3.9
TC205663 similar to UP|Q6PT61 (Q6PT61) Induced protein MgI1, par...    28  8.6

>TC210419 similar to UP|NFS1_ARATH (O49543) Cysteine desulfurase,
           mitochondrial precursor  , partial (34%)
          Length = 646

 Score =  180 bits (456), Expect(2) = 2e-77
 Identities = 90/101 (89%), Positives = 95/101 (93%)
 Frame = +1

Query: 297 IPTPLVVGMGAACEVAMKEMEYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLN 356
           +PTPLVVGMG+ACEVA  EMEYDEKRISALQ+RLLNGIREKLDGVVVNGS+ERRY GNLN
Sbjct: 1   VPTPLVVGMGSACEVAKNEMEYDEKRISALQQRLLNGIREKLDGVVVNGSLERRYVGNLN 180

Query: 357 LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALG 397
           LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYV   +G
Sbjct: 181 LSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVXEGVG 303



 Score =  127 bits (320), Expect(2) = 2e-77
 Identities = 62/65 (95%), Positives = 64/65 (98%)
 Frame = +2

Query: 394 RALGVEEDMAHTSIRFGIGRFTTEAEIDRAIQLTVHQVEKLREMSPLYEMVKEGINIKDI 453
           RALGVEEDMAHTSIRFGIGRFTTEAEIDRA++LTV QVEKLREMSPLYEMVKEGINIKDI
Sbjct: 293 RALGVEEDMAHTSIRFGIGRFTTEAEIDRAVELTVQQVEKLREMSPLYEMVKEGINIKDI 472

Query: 454 QWAQH 458
           QWAQH
Sbjct: 473 QWAQH 487


>BE024008 similar to SP|O49543|NFS1_ Cysteine desulfurase  mitochondrial
           precursor (EC 4.4.1.-). [Mouse-ear cress] {Arabidopsis
           thaliana}, partial (16%)
          Length = 271

 Score =  141 bits (355), Expect = 7e-34
 Identities = 69/90 (76%), Positives = 79/90 (87%)
 Frame = +2

Query: 369 MGLKEVAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEAEIDRAIQLTV 428
           MGLK+VAVSSGSACT+A LEPSYVLRALGVEEDMAHTSI FGIGR TT+AEIDRA++LT 
Sbjct: 2   MGLKDVAVSSGSACTTAILEPSYVLRALGVEEDMAHTSITFGIGRITTDAEIDRAVELTD 181

Query: 429 HQVEKLREMSPLYEMVKEGINIKDIQWAQH 458
            QVEKLRE++PLY MV  G+ + D+ WAQH
Sbjct: 182 QQVEKLRELNPLY*MVMVGVTVFDLSWAQH 271


>TC218074 similar to UP|Q93WX6 (Q93WX6) NIFS-like protein CpNifsp precursor
           (Cysteine desulfurase)  (At1g08490/T27G7_14) , partial
           (77%)
          Length = 1302

 Score = 90.1 bits (222), Expect = 2e-18
 Identities = 87/353 (24%), Positives = 157/353 (43%), Gaps = 22/353 (6%)
 Frame = +2

Query: 94  HFYGWESDEAVENARAQVASLIGA-SPKEIVFTSGATESNN-ISIKGVLHFYKEKKRHVI 151
           HF    +    E+AR +VAS I A   +EIVFT  A+E+ N ++    L   K     ++
Sbjct: 5   HFLSARATNEYESARRKVASFINAIDSREIVFTKNASEAINLVAYSWGLSNLKPDDEIIL 184

Query: 152 TTQTEHKCVLDSCRHLQQEGFDVTYLPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIG 211
           T    H  ++      Q+ G  + ++ +  D + D++KL+  +   T +V V  V+N + 
Sbjct: 185 TVAEHHSAIVPWQIVAQKTGAVLKFVDLNQDEIPDIDKLKEMLSRKTKIVVVHHVSNVLA 364

Query: 212 VFQPMEEIGRICKEFNVPFHTDAAQALGKVPVDVEKWNVSLMSLSGHKIYGPKGVGALYL 271
              P+ +I +   +       DA Q++  + VDV+  N   +  S HK+ GP G+G LY 
Sbjct: 365 SVLPIRDIAQWAHDVGAKVLVDACQSVPHMMVDVQSLNADFLVASSHKMCGPTGIGFLYG 544

Query: 272 RRRPRIRVEPQMNGGGQERGI--------------RSGTIPTPLVVGMGAACE----VAM 313
           +      + P + GG     +               +GT      +G+GAA +    + M
Sbjct: 545 KIDLLSSMPPFLGGGEMISDVYLDHSTYAEPPSRFEAGTPAIGEAIGLGAAIDYLSGIGM 724

Query: 314 KEM-EYDEKRISALQERLLNGIREKLDGVVVNGSMERRYAGNLNLSFAYVEGESLLMGLK 372
           + + +Y+ +    L ERLL+    ++ G   +  +ER    + N+   +    + L+  +
Sbjct: 725 QTIHDYEVELGRYLYERLLSVPNIRIYGPAPSEKVERAALCSFNVENLHPTDLATLLDQQ 904

Query: 373 E-VAVSSGSACTSASLEPSYVLRALGVEEDMAHTSIRFGIGRFTTEAEIDRAI 424
             VA+ SG  C     +P +  R LGV      +S R  +  + T+ ++D  I
Sbjct: 905 HGVAIRSGHHCA----QPLH--RYLGVS-----SSARASLYFYNTKEDVDNFI 1030


>BE210295 homologue to SP|O49543|NFS1_ Cysteine desulfurase  mitochondrial
           precursor (EC 4.4.1.-). [Mouse-ear cress] {Arabidopsis
           thaliana}, partial (5%)
          Length = 420

 Score = 78.2 bits (191), Expect = 7e-15
 Identities = 42/65 (64%), Positives = 49/65 (74%)
 Frame = +3

Query: 1   MTSKLLASTLRHHLTKPTQLLRRHFLSTAAAAAAAAEPQQHHDESAGLTMKGVKIAGRPL 60
           MTSKL+AS LRH +TK  Q LRR  LSTAAAAA   E   ++  S G+TMKGV+I+GRPL
Sbjct: 228 MTSKLIASNLRHQITKSAQNLRRQLLSTAAAAAVP-EHDGNNSNSLGMTMKGVRISGRPL 404

Query: 61  YLDVQ 65
           YLDVQ
Sbjct: 405 YLDVQ 419


>BE474515 
          Length = 455

 Score = 42.4 bits (98), Expect = 4e-04
 Identities = 34/109 (31%), Positives = 49/109 (44%), Gaps = 13/109 (11%)
 Frame = +1

Query: 177 LPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPMEEIGRICKEFNVPFHTDA-- 234
           +PV  +   D++ +R  I  +T L+   A     GV  P+EE+G +   F + FH D   
Sbjct: 40  VPVNKNFQADVKAIRRHINKNTILIVGSAPGFPHGVIDPIEELGHLASSFGICFHVDLCL 219

Query: 235 -------AQALGK--VPVDVEKWNVSLMSLSGHKIYG--PKGVGALYLR 272
                  A+ LG    P D     VS +S+  HK YG  PKG   +  R
Sbjct: 220 GGFVLPFARELGYHIPPFDFSVKGVSSISVDVHK-YGLAPKGTSVVLYR 363


>TC217615 similar to UP|SGPL_ARATH (Q9C509) Sphingosine-1-phosphate lyase
           (SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) ,
           partial (56%)
          Length = 1422

 Score = 41.6 bits (96), Expect = 8e-04
 Identities = 51/186 (27%), Positives = 75/186 (39%), Gaps = 26/186 (13%)
 Frame = +3

Query: 177 LPVESDGLVDLEKLRAAIRPDTGLVSVMAVNNEIGVFQPME--------EIGRICKEFNV 228
           +PV  +   D++ +R  I  +T L+   A     GV  P+E        E+G +   F +
Sbjct: 54  VPVNKNFQADVKAIRRHINKNTILIVGSAPGFPHGVIDPIEIIWQFLP*ELGHLASSFGI 233

Query: 229 PFHTDA---------AQALGK--VPVDVEKWNVSLMSLSGHKIYG--PKGVGALYLR--- 272
            FH D          A+ LG    P D     VS +S+  HK YG  PKG   +  R   
Sbjct: 234 CFHVDLCLGGFVLPFARELGYHIPPFDFSVKGVSSISVDVHK-YGLAPKGTSVVLYRNHE 410

Query: 273 -RRPRIRVEPQMNGGGQERGIRSGTIPTPLVVGMGAACEVAMKE-MEYDEKRISALQERL 330
            R+ +     + +GG       +G+ P  L+ G  AA     KE    + K I     R+
Sbjct: 411 IRKHQFVAVTEWSGGLYVSPTIAGSRPGSLIAGAWAAMISLGKEGYLKNTKEIMEGSRRI 590

Query: 331 LNGIRE 336
             GI E
Sbjct: 591 QQGIEE 608


>TC214997 similar to UP|Q9SS31 (Q9SS31) Calmodulin-like protein (At3g10190),
           partial (45%)
          Length = 1107

 Score = 30.4 bits (67), Expect = 1.7
 Identities = 19/51 (37%), Positives = 27/51 (52%)
 Frame = -2

Query: 22  RRHFLSTAAAAAAAAEPQQHHDESAGLTMKGVKIAGRPLYLDVQATSPVDP 72
           R H  STAAAA +      H  E++G T+ GV + G  L   V+ ++  DP
Sbjct: 344 RSHRTSTAAAATS-----DHSPETSGRTLVGVWLPGEGL*FAVEESTEEDP 207


>TC216400 homologue to UP|Q6NPD6 (Q6NPD6) At2g22125, partial (69%)
          Length = 857

 Score = 29.3 bits (64), Expect = 3.9
 Identities = 18/57 (31%), Positives = 28/57 (48%), Gaps = 3/57 (5%)
 Frame = -3

Query: 82  YISRYGNPHSRTHFY---GWESDEAVENARAQVASLIGASPKEIVFTSGATESNNIS 135
           YI+R+   H  T F    GW S    +  +A+ ASL+ A P     +  A  +NN++
Sbjct: 246 YITRFCRAHMATTFISSVGWSSSTFTKARQAETASLVRARPSF*NRSPRAKVANNLA 76


>TC205663 similar to UP|Q6PT61 (Q6PT61) Induced protein MgI1, partial (60%)
          Length = 1519

 Score = 28.1 bits (61), Expect = 8.6
 Identities = 12/36 (33%), Positives = 23/36 (63%)
 Frame = -1

Query: 86  YGNPHSRTHFYGWESDEAVENARAQVASLIGASPKE 121
           YG+ H+  H YG+  +++  ++ AQV++ I   PK+
Sbjct: 892 YGHAHAHDHCYGYWEEQSHASSEAQVSTQI-TDPKQ 788


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.318    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,772,608
Number of Sequences: 63676
Number of extensions: 226579
Number of successful extensions: 1134
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1126
length of query: 458
length of database: 12,639,632
effective HSP length: 100
effective length of query: 358
effective length of database: 6,272,032
effective search space: 2245387456
effective search space used: 2245387456
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)