BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0128.2
(265 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227939 similar to UP|Q9LK77 (Q9LK77) Similarity to acyl-CoA th... 31 0.50
TC232323 UP|PUR1_SOYBN (P52418) Amidophosphoribosyltransferase, ... 30 0.86
TC205641 UP|Q8S5C1 (Q8S5C1) 4-coumarate:CoA ligase isoenzyme 2 ... 30 0.86
TC208278 similar to UP|Q9VYT9 (Q9VYT9) CG15741-PA, partial (21%) 30 0.86
TC206671 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotrans... 30 0.86
TC225477 homologue to UP|Q6R4U3 (Q6R4U3) PPase, partial (57%) 29 1.9
TC206767 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6... 29 2.5
TC214467 UP|Q6LB28 (Q6LB28) Histone H3 variant H3.3, complete 28 3.2
AI748283 similar to PIR|S20007|S200 beta-conglycinin alpha chain... 28 4.2
TC218783 similar to UP|Q40363 (Q40363) NuM1 protein, partial (19%) 28 4.2
TC233531 similar to UP|Q84U29 (Q84U29) Zinc finger-like protein ... 28 4.2
TC230134 weakly similar to UP|NUCL_HUMAN (P19338) Nucleolin (Pro... 28 4.2
BF595056 28 5.5
TC213512 28 5.5
BE661927 28 5.5
TC207256 similar to UP|Q6YC80 (Q6YC80) Lecithin diacylglycerol c... 28 5.5
TC229651 28 5.5
BF595007 similar to PIR|S57766|S577 dioscorin class A precursor ... 27 7.2
TC216972 similar to UP|OXA1_ARATH (Q42191) Inner membrane protei... 27 7.2
TC217730 similar to GB|AAQ56808.1|34098851|BT010365 At5g07720 {A... 27 7.2
>TC227939 similar to UP|Q9LK77 (Q9LK77) Similarity to acyl-CoA thioesterase,
partial (84%)
Length = 1459
Score = 31.2 bits (69), Expect = 0.50
Identities = 31/120 (25%), Positives = 45/120 (36%), Gaps = 13/120 (10%)
Frame = +1
Query: 120 RTSDKCIDDKCLFRSVWNSVQSLKNNRLTARGFTRVNAETGHNDDLSLNIAP----CD-- 173
R S + +K LF ++ N R T RG + + E G + L +A CD
Sbjct: 478 RLSPENAHEKLLFEQA----EARSNLRKTKRGGEKRDFENGEKNRLRALLAEGRIFCDMP 645
Query: 174 ---DNEVVTLKPSGADG----EPAWFYLHGYIFNELFMKLPFSTFVCDVLTFLNVAPCQL 226
D + LK + + +P +HG IF M F F +APC L
Sbjct: 646 ALADRNSILLKDTSLENALICQPQQRNIHGRIFGGFLMHRAFELAFSTAYAFAGLAPCFL 825
>TC232323 UP|PUR1_SOYBN (P52418) Amidophosphoribosyltransferase, chloroplast
precursor (Glutamine phosphoribosylpyrophosphate
amidotransferase) (ATASE) (GPAT) , partial (72%)
Length = 1231
Score = 30.4 bits (67), Expect = 0.86
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = +1
Query: 72 TTVSQEVITVDSDSESHNSPSSSDPTQKRTVSITSHLSTGNPSTSEDW 119
T S + DS + +PSS+ P R S +S L S+SE W
Sbjct: 463 TATSSTTAPSEPDSNTTTAPSSTPPPTPRLSSTSSPLQNTGHSSSESW 606
>TC205641 UP|Q8S5C1 (Q8S5C1) 4-coumarate:CoA ligase isoenzyme 2 , complete
Length = 1946
Score = 30.4 bits (67), Expect = 0.86
Identities = 16/58 (27%), Positives = 28/58 (47%)
Frame = -1
Query: 96 PTQKRTVSITSHLSTGNPSTSEDWRTSDKCIDDKCLFRSVWNSVQSLKNNRLTARGFT 153
P + + +S L + + STS +S +C +C F SVW +S + ++ R T
Sbjct: 650 PEE*GNATTSSGLISTSTSTSASPLSSHRCEKWRCCFSSVWGGQESTNTSLMSRRSLT 477
>TC208278 similar to UP|Q9VYT9 (Q9VYT9) CG15741-PA, partial (21%)
Length = 1428
Score = 30.4 bits (67), Expect = 0.86
Identities = 19/55 (34%), Positives = 28/55 (50%)
Frame = +2
Query: 62 LNSDSDDGDKTTVSQEVITVDSDSESHNSPSSSDPTQKRTVSITSHLSTGNPSTS 116
L + D+G+ S VIT SE++ + S P QK ++ TS S+ STS
Sbjct: 746 LEDNDDEGEADAAS--VITTSEWSETYQNLISPSPVQKPVLTTTSPTSSTTSSTS 904
>TC206671 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransferase-like
protein (At5g07010), partial (40%)
Length = 1308
Score = 30.4 bits (67), Expect = 0.86
Identities = 22/60 (36%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = +3
Query: 207 PFSTFVCDVLTFLNVAPCQLLPNAWGFTRCFELLCEPL-NFGPPYPLFLFFYKISIAKPS 265
P TFV + FLN + LP + FE C+ + FGP + L ++K SIA+PS
Sbjct: 573 PLDTFVSTWI-FLNKIKPEHLPE-FELGEAFEKYCKGIIGFGPTWDQMLGYWKESIARPS 746
>TC225477 homologue to UP|Q6R4U3 (Q6R4U3) PPase, partial (57%)
Length = 1488
Score = 29.3 bits (64), Expect = 1.9
Identities = 23/101 (22%), Positives = 45/101 (43%), Gaps = 10/101 (9%)
Frame = -2
Query: 74 VSQEVITVDSDSESHNSPSSSDPTQKRTVSITSHLST----------GNPSTSEDWRTSD 123
VS I + + + +SP ++ T+++ ++ LST NP+ + + + +
Sbjct: 320 VSIACIVLCNKPNNKSSP*TNTHTEEQLPALYFLLSTKVENCEGCWQSNPTNNCNSNSHE 141
Query: 124 KCIDDKCLFRSVWNSVQSLKNNRLTARGFTRVNAETGHNDD 164
C DDK LF S +N + SL ++ + + H D
Sbjct: 140 YCRDDKLLF*SWFNFLDSLDLKEISCKESSNQTDNNAHRTD 18
>TC206767 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-phosphate
reductase, complete
Length = 1323
Score = 28.9 bits (63), Expect = 2.5
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +3
Query: 98 QKRTVSITSHLSTGNPSTSEDWRTSDKCIDDKCL 131
QK + +T+H G + + +W + C+DD+ L
Sbjct: 636 QKHGICVTAHTPLGGAAANAEWFGTVSCLDDQVL 737
>TC214467 UP|Q6LB28 (Q6LB28) Histone H3 variant H3.3, complete
Length = 835
Score = 28.5 bits (62), Expect = 3.2
Identities = 15/35 (42%), Positives = 21/35 (59%)
Frame = +2
Query: 13 HHCSLHSYYFTHPRLVPLFSLYFSCFSIHLLSMKL 47
H+ S+ S+Y R V LY++ FS HL+S KL
Sbjct: 683 HNVSVFSFYL---RDVVTLELYWTTFSCHLISFKL 778
>AI748283 similar to PIR|S20007|S200 beta-conglycinin alpha chain precursor -
soybean, partial (5%)
Length = 448
Score = 28.1 bits (61), Expect = 4.2
Identities = 17/41 (41%), Positives = 21/41 (50%)
Frame = -2
Query: 83 SDSESHNSPSSSDPTQKRTVSITSHLSTGNPSTSEDWRTSD 123
S S S +S SSS P RT S+T+H + S S W D
Sbjct: 444 SCSSSSSSSSSSQPPWPRTSSMTAHFCPSHDSCS--WSQQD 328
>TC218783 similar to UP|Q40363 (Q40363) NuM1 protein, partial (19%)
Length = 870
Score = 28.1 bits (61), Expect = 4.2
Identities = 26/90 (28%), Positives = 39/90 (42%), Gaps = 12/90 (13%)
Frame = +3
Query: 46 KLPKMSAK*QSYYSLSLNSDSDDGDKTTVSQEV------------ITVDSDSESHNSPSS 93
K K +A S S NSD D+ KT V+ + SDS+S +S SS
Sbjct: 180 KKGKPAASSSSSESSDDNSDEDEAPKTKVAPAAGKNGHASTKKTQPSESSDSDSSDSDSS 359
Query: 94 SDPTQKRTVSITSHLSTGNPSTSEDWRTSD 123
SD + + T+ L T + ++ +SD
Sbjct: 360 SDEGKSKKKPTTAKLPTLPVAPAKKVESSD 449
>TC233531 similar to UP|Q84U29 (Q84U29) Zinc finger-like protein (Fragment),
partial (68%)
Length = 926
Score = 28.1 bits (61), Expect = 4.2
Identities = 27/116 (23%), Positives = 44/116 (37%)
Frame = +2
Query: 49 KMSAK*QSYYSLSLNSDSDDGDKTTVSQEVITVDSDSESHNSPSSSDPTQKRTVSITSHL 108
+M A S YSL+ D K ++E ++++E+ S S Q
Sbjct: 197 QMKAGNSSRYSLASLMLGDVVFKKVEAEEAEEAEAEAEASCELSRSRTLQDIIREEKPSN 376
Query: 109 STGNPSTSEDWRTSDKCIDDKCLFRSVWNSVQSLKNNRLTARGFTRVNAETGHNDD 164
S+ N WR + K S W+S ++ N+ R + ET H+ D
Sbjct: 377 SSNNTKDRNSWRAFTAKLRLKRAIGSAWSSTTTITNHSNDDRNLPNI-PETRHDSD 541
>TC230134 weakly similar to UP|NUCL_HUMAN (P19338) Nucleolin (Protein C23),
partial (8%)
Length = 581
Score = 28.1 bits (61), Expect = 4.2
Identities = 19/56 (33%), Positives = 30/56 (52%)
Frame = -3
Query: 59 SLSLNSDSDDGDKTTVSQEVITVDSDSESHNSPSSSDPTQKRTVSITSHLSTGNPS 114
SLS +S S +++S + S SES +SP SS +++ S +S S +PS
Sbjct: 468 SLSSSSSSSSSSSSSLSPPPLASGSSSESASSPPSS---PEKSSSSSSSSSPSSPS 310
>BF595056
Length = 421
Score = 27.7 bits (60), Expect = 5.5
Identities = 25/100 (25%), Positives = 45/100 (45%), Gaps = 7/100 (7%)
Frame = +1
Query: 59 SLSLNSDSDDGDKTTVSQEVITVDSDSESHNSPSSSDPTQ-KRTVSITS------HLSTG 111
S S +S S ++ + Q +IT+ + + + N+PSSS + +RT+S H T
Sbjct: 112 SSSSSSSSVRIEEEIMKQGIITILNSNTNTNTPSSSSSSSLRRTLSADMSSKNYLHAKTI 291
Query: 112 NPSTSEDWRTSDKCIDDKCLFRSVWNSVQSLKNNRLTARG 151
S E+ D+ ++ +W+S+Q K G
Sbjct: 292 ADSEKEEEDDEDEAERERL---EIWSSIQRNKKEEQEKSG 402
>TC213512
Length = 560
Score = 27.7 bits (60), Expect = 5.5
Identities = 14/37 (37%), Positives = 21/37 (55%), Gaps = 1/37 (2%)
Frame = +3
Query: 87 SHNSPSS-SDPTQKRTVSITSHLSTGNPSTSEDWRTS 122
+ +SPSS SDP +R+ T PS+ E+W+ S
Sbjct: 330 ARSSPSSGSDPRTRRSTMPGQSWPTSRPSSPENWKRS 440
>BE661927
Length = 768
Score = 27.7 bits (60), Expect = 5.5
Identities = 18/63 (28%), Positives = 28/63 (43%)
Frame = +3
Query: 190 AWFYLHGYIFNELFMKLPFSTFVCDVLTFLNVAPCQLLPNAWGFTRCFELLCEPLNFGPP 249
++F+ H ++F + PF C + + AP + P T + LL PLN P
Sbjct: 330 SFFHSHHFLF---LLPPPFCCCCCCLFINYHHAPQTIQPKLATLTTLWPLLLXPLNGLSP 500
Query: 250 YPL 252
PL
Sbjct: 501 IPL 509
>TC207256 similar to UP|Q6YC80 (Q6YC80) Lecithin diacylglycerol cholesterol
acyltransferase, partial (86%)
Length = 1846
Score = 27.7 bits (60), Expect = 5.5
Identities = 16/59 (27%), Positives = 25/59 (42%)
Frame = +3
Query: 10 CSDHHCSLHSYYFTHPRLVPLFSLYFSCFSIHLLSMKLPKMSAK*QSYYSLSLNSDSDD 68
C D H F R V +Y + + LL PKM A+ +++S + + DD
Sbjct: 720 CRDVWTEYHEMGFEGVRAVAEHKVYTAGSVVDLLQFVAPKMMARGSAHFSYGIADNLDD 896
>TC229651
Length = 1257
Score = 27.7 bits (60), Expect = 5.5
Identities = 16/45 (35%), Positives = 24/45 (52%)
Frame = -3
Query: 4 IPVTFQCSDHHCSLHSYYFTHPRLVPLFSLYFSCFSIHLLSMKLP 48
+PV F + C + SY+ ++P VP+F + SC S LL P
Sbjct: 520 LPVIFS---NCCPIFSYFISYPHSVPVF-VNISCHSNSLLHKWFP 398
>BF595007 similar to PIR|S57766|S577 dioscorin class A precursor - Dioscorea
cayenensis, partial (6%)
Length = 413
Score = 27.3 bits (59), Expect = 7.2
Identities = 20/50 (40%), Positives = 25/50 (50%)
Frame = +2
Query: 13 HHCSLHSYYFTHPRLVPLFSLYFSCFSIHLLSMKLPKMSAK*QSYYSLSL 62
H CSLH F R++PL LY LL+ L +S SY S+SL
Sbjct: 194 HLCSLHLSLFLSFRILPLCCLYSFLVQHVLLACSL*SVS----SYPSVSL 331
>TC216972 similar to UP|OXA1_ARATH (Q42191) Inner membrane protein OXA1,
mitochondrial precursor (Oxidase assembly 1 protein)
(AtOXA1), partial (32%)
Length = 705
Score = 27.3 bits (59), Expect = 7.2
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +2
Query: 82 DSDSESHNSPSSSDPTQKRTVSITSHLSTGNPSTSEDWRTSDKC 125
+S ++SHN+PSS+ Q T+S+ ST S W + C
Sbjct: 329 ESSADSHNTPSSTH--QWSTISLFYISSTKTSYVSNKWTKLNAC 454
>TC217730 similar to GB|AAQ56808.1|34098851|BT010365 At5g07720 {Arabidopsis
thaliana;} , partial (46%)
Length = 950
Score = 27.3 bits (59), Expect = 7.2
Identities = 17/51 (33%), Positives = 25/51 (48%), Gaps = 1/51 (1%)
Frame = -3
Query: 73 TVSQEVITVDSDSESHNSPSSSDPTQKRT-VSITSHLSTGNPSTSEDWRTS 122
T + +T+ S+S SP + PT+ S T+ T PSTS W T+
Sbjct: 408 TATSRPLTLGDTSQS--SPHTDQPTRPNNRASRTNSQETSCPSTSPSWTTA 262
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.137 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,604,037
Number of Sequences: 63676
Number of extensions: 259544
Number of successful extensions: 1918
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 1901
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1917
length of query: 265
length of database: 12,639,632
effective HSP length: 96
effective length of query: 169
effective length of database: 6,526,736
effective search space: 1103018384
effective search space used: 1103018384
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0128.2