BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0125.16
(229 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228623 similar to UP|Q9SA21 (Q9SA21) F3O9.2 protein, partial (... 37 0.007
TC205705 similar to GB|AAP37784.1|30725524|BT008425 At4g24690 {A... 34 0.048
TC205438 similar to UP|TUL1_HUMAN (O00294) Tubby related protein... 33 0.082
TC215270 similar to PIR|T01729|T01729 mitochondrial solute carri... 33 0.11
TC225036 similar to UP|O65357 (O65357) Aquaporin 2, partial (92%) 33 0.14
AI494721 33 0.14
TC224128 weakly similar to PRF|1604369A.0|226743|1604369A sulfat... 32 0.24
TC206672 weakly similar to UP|Q7UTL3 (Q7UTL3) 3-hydroxyisobutyra... 32 0.31
BE609293 32 0.31
TC211828 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-pho... 32 0.31
TC205920 similar to UP|14P_ARATH (O64628) UPF0071 protein At2g18... 31 0.41
BM086218 similar to PIR|T04107|T041 calmodulin-binding heat-shoc... 31 0.53
TC214871 homologue to UP|RUBB_PEA (P08927) RuBisCO subunit bindi... 31 0.53
TC233156 weakly similar to UP|Q6K270 (Q6K270) Nodulation recepto... 31 0.53
TC230795 weakly similar to UP|O23299 (O23299) Carnitine racemase... 31 0.53
TC211029 similar to UP|Q96VI4 (Q96VI4) Protease 1, partial (3%) 30 0.70
BU090189 30 0.70
AW703638 weakly similar to GP|9758683|dbj| tyrosine-specific pro... 30 0.91
BU761594 similar to GP|22654971|gb At1g80930/F23A5_23 {Arabidops... 30 0.91
TC214758 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-pho... 30 0.91
>TC228623 similar to UP|Q9SA21 (Q9SA21) F3O9.2 protein, partial (85%)
Length = 1082
Score = 37.0 bits (84), Expect = 0.007
Identities = 29/93 (31%), Positives = 40/93 (42%), Gaps = 15/93 (16%)
Frame = -3
Query: 80 LPPSVRCG----PGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVAS-- 133
LPPS P + + PP+PP S+S PS A PV+P + + S
Sbjct: 510 LPPSDSVAS*PPPPSSQPPPAPPPSASSAPPPSP-SAPGSTPVSPSCPPSSSTSPAPSPP 334
Query: 134 ---------SSRLVEPKREPVVPSASRRFSFTP 157
S+RL P P+ PS+SRR + P
Sbjct: 333 APPTVAPPLSARLPPPSFRPLPPSSSRRVTLNP 235
>TC205705 similar to GB|AAP37784.1|30725524|BT008425 At4g24690 {Arabidopsis
thaliana;} , partial (40%)
Length = 1787
Score = 34.3 bits (77), Expect = 0.048
Identities = 44/146 (30%), Positives = 63/146 (43%), Gaps = 12/146 (8%)
Frame = +3
Query: 94 PPSPPSSSSDEEDPSEIE-------ADVDAPVAPLPATAVDLTDVASSS---RLVE-PKR 142
P P + DEE E+E A APVA PA T VASSS +++ +
Sbjct: 1056 PIEPLNQMVDEEPRLELENEFPINEATFVAPVASAPAA----TSVASSSVPCPIIDLSET 1223
Query: 143 EPVVPSASRRFSF-TPSRGYRVELLVRVVEEDVLSEEVDVEMGSTRVVRRIVRKEVMDLD 201
P VPS + + PS + VEE++L E ++ + + I+RK DLD
Sbjct: 1224 TPAVPSNQQSPTVDVPSSSMGTGGGINSVEENLLKELEEMGFKQVDLNKEILRKNEYDLD 1403
Query: 202 TEMITVDSDSDTDVDNYSPSDEEEEE 227
++D+ DV S D EE
Sbjct: 1404 Q---SLDALCGVDVCGVSEWDHMLEE 1472
>TC205438 similar to UP|TUL1_HUMAN (O00294) Tubby related protein 1
(Tubby-like protein 1), partial (14%)
Length = 890
Score = 33.5 bits (75), Expect = 0.082
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = +3
Query: 63 RGRLTRGVLRRMELSFNLPPSVRCGPGTGRGPPSPPSSSSDEE 105
RGR+T ++ +L + PP+ GTG P P SSSD +
Sbjct: 324 RGRVTVASVKNFQLIASTPPAAVAPTGTGPTPSQPTQSSSDPD 452
>TC215270 similar to PIR|T01729|T01729 mitochondrial solute carrier protein
homolog - Arabidopsis thaliana {Arabidopsis thaliana;} ,
partial (31%)
Length = 585
Score = 33.1 bits (74), Expect = 0.11
Identities = 18/40 (45%), Positives = 21/40 (52%)
Frame = +1
Query: 94 PPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVAS 133
PPSPPSS S S +A P P PA+A + VAS
Sbjct: 223 PPSPPSSISPRRPSSPEKASSKLPATPSPASASRSSPVAS 342
>TC225036 similar to UP|O65357 (O65357) Aquaporin 2, partial (92%)
Length = 1610
Score = 32.7 bits (73), Expect = 0.14
Identities = 25/78 (32%), Positives = 29/78 (37%), Gaps = 6/78 (7%)
Frame = +1
Query: 54 RVMEDRTDER----GRLTRGVLRRMELSFNLPPSVRCGPGTGRGPP--SPPSSSSDEEDP 107
R+ + T +R G LTR PPS GP + P SPPSSSS
Sbjct: 469 RIRHEETSKRRFKVGCLTRTTTTHPPPHSTTPPSSESGPSSEPSSPSSSPPSSSSTSPSS 648
Query: 108 SEIEADVDAPVAPLPATA 125
P P PA A
Sbjct: 649 PSSATTTRQPPPPSPAAA 702
>AI494721
Length = 253
Score = 32.7 bits (73), Expect = 0.14
Identities = 21/76 (27%), Positives = 31/76 (40%)
Frame = +3
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEP 140
PP C P + PPSPP SS+ P+ + P + +SS+R P
Sbjct: 30 PPPNPCAPP--KSPPSPPPSSASPRAPTS*S*ASPTSPSSGPTSTTAAAAPSSSTRTNTP 203
Query: 141 KREPVVPSASRRFSFT 156
R +PS R ++
Sbjct: 204 SRNSSLPSPRRSLRYS 251
>TC224128 weakly similar to PRF|1604369A.0|226743|1604369A sulfated surface
glycoprotein SSG185. {Volvox carteri;} , partial (9%)
Length = 633
Score = 32.0 bits (71), Expect = 0.24
Identities = 19/44 (43%), Positives = 20/44 (45%)
Frame = -1
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPAT 124
PP R P T PP PSSSS + PS P P PAT
Sbjct: 174 PPKQRNYPHTPPTPPHTPSSSSPQSPPSPXPXPASPP--PSPAT 49
>TC206672 weakly similar to UP|Q7UTL3 (Q7UTL3) 3-hydroxyisobutyrate
dehydrogenase , partial (76%)
Length = 1230
Score = 31.6 bits (70), Expect = 0.31
Identities = 21/64 (32%), Positives = 27/64 (41%), Gaps = 1/64 (1%)
Frame = +2
Query: 88 PGTGRGPPSP-PSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVV 146
P + PP P PSS+S P AP + P++A T SSS P
Sbjct: 164 PSSTAPPPRPSPSSTSGPTSPPPPTPSQPAPTSSSPSSATPRTSAQSSSTPPPAPSPPSA 343
Query: 147 PSAS 150
P+AS
Sbjct: 344 PAAS 355
>BE609293
Length = 436
Score = 31.6 bits (70), Expect = 0.31
Identities = 23/65 (35%), Positives = 27/65 (41%)
Frame = +1
Query: 88 PGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVP 147
P T PPSPPS S+ P +P PAT+ T A+S P P
Sbjct: 7 PSTPTQPPSPPSPST------------PLPTSPSPATSSPPTPAATS-----PSSTPTAL 135
Query: 148 SASRR 152
S SRR
Sbjct: 136 STSRR 150
>TC211828 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-phosphate
isomerase (Phosphoriboisomerase) , partial (47%)
Length = 729
Score = 31.6 bits (70), Expect = 0.31
Identities = 22/78 (28%), Positives = 34/78 (43%)
Frame = +2
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEP 140
PPS + + PP+ PSS+S P+ A AP+ P+TA + +S+
Sbjct: 347 PPSSSPKTTSRKSPPTRPSSTSS---PAWSSASAPAPLPSTPSTASASSSAKENSKTSSE 517
Query: 141 KREPVVPSASRRFSFTPS 158
P P+ S +PS
Sbjct: 518 SPPPPKPTTRPSPSASPS 571
>TC205920 similar to UP|14P_ARATH (O64628) UPF0071 protein At2g18990, partial
(86%)
Length = 985
Score = 31.2 bits (69), Expect = 0.41
Identities = 22/55 (40%), Positives = 27/55 (49%)
Frame = -3
Query: 48 RATYRGRVMEDRTDERGRLTRGVLRRMELSFNLPPSVRCGPGTGRGPPSPPSSSS 102
RA++RGR R R+ RR LS ++R G G G SPPSSSS
Sbjct: 416 RASFRGR------SGRRRVRWP*RRRRSLSRRESRNIRSGRGRSSGSASPPSSSS 270
>BM086218 similar to PIR|T04107|T041 calmodulin-binding heat-shock protein -
common tobacco, partial (8%)
Length = 430
Score = 30.8 bits (68), Expect = 0.53
Identities = 17/44 (38%), Positives = 24/44 (53%)
Frame = +2
Query: 92 RGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSS 135
RGPP PP+SS+ + + +P++ P TA T ASSS
Sbjct: 212 RGPPPPPTSSTRSHASAASSSPTTSPISVPPTTA--STPTASSS 337
>TC214871 homologue to UP|RUBB_PEA (P08927) RuBisCO subunit binding-protein
beta subunit, chloroplast precursor (60 kDa chaperonin
beta subunit) (CPN-60 beta), partial (23%)
Length = 542
Score = 30.8 bits (68), Expect = 0.53
Identities = 22/63 (34%), Positives = 28/63 (43%), Gaps = 4/63 (6%)
Frame = +2
Query: 80 LPPSVRCGPGTG-RGPPSPPSSSSDEEDPS---EIEADVDAPVAPLPATAVDLTDVASSS 135
LPPS++C P + PPSPPS + PS A +P P T+ SS
Sbjct: 101 LPPSLQCPPASSPPPPPSPPSPPAAAPTPSFCRGAAAPPGSPPWPRSCTSTKTAPQLRSS 280
Query: 136 RLV 138
R V
Sbjct: 281 RAV 289
>TC233156 weakly similar to UP|Q6K270 (Q6K270) Nodulation receptor
kinase-like protein, partial (9%)
Length = 629
Score = 30.8 bits (68), Expect = 0.53
Identities = 32/114 (28%), Positives = 43/114 (37%), Gaps = 3/114 (2%)
Frame = +2
Query: 81 PPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEP 140
PP V P + PPSPP+ + PS + D P A P
Sbjct: 119 PPPVTPPPSSAPPPPSPPTGTPSPPSPS--PSPEDEPGA-------------------SP 235
Query: 141 KREPVVPSAS---RRFSFTPSRGYRVELLVRVVEEDVLSEEVDVEMGSTRVVRR 191
+R P P+AS R S P EL+VR E + EV + +T R+
Sbjct: 236 RRIPTTPTASGIPRSSSLLPLYQMLPELVVR---EGMARNEVPASVHTTYYQRK 388
>TC230795 weakly similar to UP|O23299 (O23299) Carnitine racemase like
protein (AT4g14430/dl3255c), partial (47%)
Length = 749
Score = 30.8 bits (68), Expect = 0.53
Identities = 23/74 (31%), Positives = 30/74 (40%), Gaps = 6/74 (8%)
Frame = +3
Query: 80 LPPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAP------VAPLPATAVDLTDVAS 133
LP S+R + + PP+PPSS D S + P P A+A T A
Sbjct: 72 LPSSLRSPKSSPKPPPAPPSSPPRTADSSATASTSAGPRPPATSPPPASASAACPTPSAP 251
Query: 134 SSRLVEPKREPVVP 147
SS P P +P
Sbjct: 252 SSPRSSPSPSPPLP 293
>TC211029 similar to UP|Q96VI4 (Q96VI4) Protease 1, partial (3%)
Length = 858
Score = 30.4 bits (67), Expect = 0.70
Identities = 21/76 (27%), Positives = 37/76 (48%)
Frame = +1
Query: 76 LSFNLPPSVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSS 135
L LPP+ PG+ PP+ +S+S +P+ +AP P+++ L +S+
Sbjct: 352 LPLTLPPT----PGSSSQPPNTTTSTSTNTNPT------PPSLAPTPSSS-SLPPTSSTP 498
Query: 136 RLVEPKREPVVPSASR 151
+ P P +PS S+
Sbjct: 499 QFSTPHGTPPLPSTSQ 546
>BU090189
Length = 307
Score = 30.4 bits (67), Expect = 0.70
Identities = 16/50 (32%), Positives = 22/50 (44%)
Frame = +3
Query: 58 DRTDERGRLTRGVLRRMELSFNLPPSVRCGPGTGRGPPSPPSSSSDEEDP 107
D DE R ++ R+ S LP C PG G S+ +E+DP
Sbjct: 141 DHPDELKRRRDRIVERLYASSALPQCRNCDPGAGEIRTQSSPSAEEEKDP 290
>AW703638 weakly similar to GP|9758683|dbj| tyrosine-specific protein
phosphatase-like protein {Arabidopsis thaliana}, partial
(6%)
Length = 404
Score = 30.0 bits (66), Expect = 0.91
Identities = 14/31 (45%), Positives = 18/31 (57%), Gaps = 2/31 (6%)
Frame = +3
Query: 80 LPPSVRCGPGTGRGPPSPPSSS--SDEEDPS 108
LPPS G GR PPSPP + ++ E P+
Sbjct: 183 LPPSPSSSNGDGRNPPSPPKPA*RTESETPT 275
>BU761594 similar to GP|22654971|gb At1g80930/F23A5_23 {Arabidopsis
thaliana}, partial (13%)
Length = 397
Score = 30.0 bits (66), Expect = 0.91
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +1
Query: 207 VDSDSDTDVDNYSPSDEEEEEEL 229
+DS+SD D D SDEEEEE++
Sbjct: 214 LDSESDDDDDEDDESDEEEEEQM 282
>TC214758 similar to UP|RPIA_ARATH (Q9ZU38) Probable-ribose 5-phosphate
isomerase (Phosphoriboisomerase) , partial (79%)
Length = 836
Score = 30.0 bits (66), Expect = 0.91
Identities = 29/110 (26%), Positives = 41/110 (36%), Gaps = 19/110 (17%)
Frame = +3
Query: 68 RGVLRRMELSFNLPPSVRCGPG------TGRGPPSPPSSS-------------SDEEDPS 108
RG L+ + L++ P + P PP PPSSS S P+
Sbjct: 54 RG*LQPLTLTWPFPTPISSPPRKPPWTPASSTPPPPPSSSPKTI*RKSPPTRPSSTWSPA 233
Query: 109 EIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSASRRFSFTPS 158
A AP +P+TA + +SSR P P+ S +PS
Sbjct: 234 *SSASAPAPPPSMPSTASASSSARASSRTSSESPPPQKPTNKPSPSGSPS 383
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.315 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,852,489
Number of Sequences: 63676
Number of extensions: 133780
Number of successful extensions: 1537
Number of sequences better than 10.0: 135
Number of HSP's better than 10.0 without gapping: 1359
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1494
length of query: 229
length of database: 12,639,632
effective HSP length: 94
effective length of query: 135
effective length of database: 6,654,088
effective search space: 898301880
effective search space used: 898301880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0125.16