BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0122.9
(460 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP004759 polygalacturonase PG1 372 e-103
TC221920 UP|Q9SWS2 (Q9SWS2) Polygalacturonase PG2, complete 365 e-101
NP501836 dehiscence-related endopolygalaturonase [Glycine max] 295 3e-80
BE346184 223 2e-58
CA801781 216 2e-56
TC231585 weakly similar to UP|Q9M7D3 (Q9M7D3) Polygalacturonase,... 197 6e-51
TC208008 homologue to UP|Q94KI6 (Q94KI6) Polygalacturonase (Frag... 179 3e-45
TC212447 weakly similar to UP|O82586 (O82586) Endopolygalacturon... 111 2e-36
TC231081 weakly similar to UP|Q9M7D3 (Q9M7D3) Polygalacturonase,... 127 1e-35
TC222699 weakly similar to UP|Q9LW07 (Q9LW07) Polygalacturonase-... 122 3e-28
CA853310 110 1e-27
BG650346 similar to GP|6624205|dbj polygalacturonase {Cucumis sa... 114 7e-26
TC234738 similar to UP|Q94EH4 (Q94EH4) At1g48100/F21D18_17, part... 88 6e-20
TC217817 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 93 2e-19
TC217917 similar to UP|Q9M1L0 (Q9M1L0) Polygalacturonase-like pr... 86 3e-17
TC205977 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 82 4e-16
TC218204 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like pr... 80 1e-15
TC206561 75 5e-14
TC218721 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like pr... 62 9e-13
TC223574 homologue to UP|Q9SWS2 (Q9SWS2) Polygalacturonase PG2, ... 69 6e-12
>NP004759 polygalacturonase PG1
Length = 1332
Score = 372 bits (954), Expect = e-103
Identities = 184/404 (45%), Positives = 265/404 (65%), Gaps = 6/404 (1%)
Frame = +1
Query: 63 FDVTTFGAVGDGIADDTVSFKMAWDTACQSELPGNVILVPKGFSFIIQSTIFTGP-CQGG 121
F+V +GA GDG ADDT +F+ AW AC+ E G+ ++VP G F+++ F+GP C+
Sbjct: 136 FNVLDYGAKGDGHADDTKAFEDAWAAACKVE--GSTMVVPSGSVFLVKPISFSGPNCEPN 309
Query: 122 IVFKVEGTLMPPDGPESWPKNISRHQWLVFYRINGMSLEGSGLIDGRGEKWWDLPCKPHR 181
IVF+++G ++ P E+W QWL F ++N +++ G G+IDG+G WW+ +
Sbjct: 310 IVFQLDGKIIAPTSSEAWGSGTL--QWLEFSKLNTITIRGKGVIDGQGSVWWNNDSPTYN 483
Query: 182 QGPHWTTLPG--PCDSPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKVHVESIYITA 239
G P P A+RF+ S +TV G+ ++NS Q + +FD C V V I +++
Sbjct: 484 PTEVMLESNGRLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDSCTNVQVSGISVSS 663
Query: 240 PKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSGCYDVDIKNITCGPGHGISIGSLG 299
P SPNTDGIH++N+ +V IY+S ++ GDDCVSI +GC D+ + N+ CGPGHGISIGSLG
Sbjct: 664 PGDSPNTDGIHLQNSQNVVIYSSTLACGDDCVSIQTGCSDIYVHNVNCGPGHGISIGSLG 843
Query: 300 IHNSRACVSNITVRDSMMKGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNPIIIDQFY 359
N++ACV N+TVRD ++ T GVRIKTWQGGSGSV + FSN+++ V+ PI IDQ+Y
Sbjct: 844 RENTKACVRNVTVRDVTIQNTLTGVRIKTWQGGSGSVQNIMFSNVQVSGVQTPISIDQYY 1023
Query: 360 CITEGCTNKTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPA-EG 418
C C N++SAV VS I Y N+KGTY P+ FACSD++PC+ + L I+L A E
Sbjct: 1024CDGGRCRNESSAVAVSGIHYVNVKGTYTKE--PIYFACSDNLPCSGITLDTIQLESAQET 1197
Query: 419 DIAHDPFCWNAYGDLQTLTIPPVSCLMQGTP--QSWLDHDINGC 460
++ PFCW AYG+L+T T+PPV CL +G P + ++ +I+ C
Sbjct: 1198KNSNVPFCWEAYGELKTTTVPPVECLQRGNPSKEGGINSNIDSC 1329
>TC221920 UP|Q9SWS2 (Q9SWS2) Polygalacturonase PG2, complete
Length = 1660
Score = 365 bits (937), Expect = e-101
Identities = 181/391 (46%), Positives = 257/391 (65%), Gaps = 4/391 (1%)
Frame = +1
Query: 63 FDVTTFGAVGDGIADDTVSFKMAWDTACQSELPGNVILVPKGFSFIIQSTIFTGP-CQGG 121
F+V +GA G ADDT +F+ AW AC+ E G+ ++VP G F+++ F+GP C+
Sbjct: 208 FNVLDYGAKDGGHADDTKAFQNAWVAACKVE--GSTMVVPSGSVFLVKPISFSGPNCEPN 381
Query: 122 IVFKVEGTLMPPDGPESWPKNISRHQWLVFYRINGMSLEGSGLIDGRGEKWWDLPCKPHR 181
IVF+++G ++ P G +W QWL F ++N +++ G G+IDG+G WW+ +
Sbjct: 382 IVFQLDGKIIAPTGSAAWGSGTL--QWLEFSKLNKITIRGKGVIDGQGSVWWNNDSPTYN 555
Query: 182 QGPHWTTLPG--PCDSPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKVHVESIYITA 239
G P P A+RF+ S +TV G+ ++NS Q + +FD C V V I +++
Sbjct: 556 PTEVMLESNGRLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDSCTNVQVSGISVSS 735
Query: 240 PKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSGCYDVDIKNITCGPGHGISIGSLG 299
P SPNTDGIH++N+ +V IY+S ++ GDDC+SI +GC D+ + N+ CGPGHGISIGSLG
Sbjct: 736 PGDSPNTDGIHLQNSQNVVIYSSTLACGDDCISIQTGCSDIYVHNVNCGPGHGISIGSLG 915
Query: 300 IHNSRACVSNITVRDSMMKGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNPIIIDQFY 359
N++ACV N+TVRD ++ T GVRIKTWQGGSGSV + FSN+++ V+ PI+IDQ+Y
Sbjct: 916 RENTKACVRNVTVRDVTIQNTLTGVRIKTWQGGSGSVQNIMFSNVQVSGVQIPILIDQYY 1095
Query: 360 CITEGCTNKTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPA-EG 418
C C N++SAV VS I Y NIKGTY + P+ FACSD++PCT + L I L A E
Sbjct: 1096CDGGKCRNESSAVAVSAIHYVNIKGTYTKQ--PIYFACSDNLPCTGITLDTIRLESAQET 1269
Query: 419 DIAHDPFCWNAYGDLQTLTIPPVSCLMQGTP 449
++ PFCW AYG+L+T T+PPV CL +G P
Sbjct: 1270KNSNVPFCWEAYGELKTKTVPPVECLQRGNP 1362
>NP501836 dehiscence-related endopolygalaturonase [Glycine max]
Length = 1287
Score = 295 bits (755), Expect = 3e-80
Identities = 156/352 (44%), Positives = 216/352 (61%), Gaps = 2/352 (0%)
Frame = +1
Query: 64 DVTTFGAVGDGIADDTVSFKMAWDTACQSELPGNVILVPKGFSFIIQSTIFTGPCQGGIV 123
+V +GA GDG DDT +FK AW+ AC S G V +VP+ +++++ F+GPC+ I
Sbjct: 187 NVNDYGAPGDGKTDDTQAFKEAWEVACSSG--GAVFVVPRK-NYLLKPFTFSGPCESDIE 357
Query: 124 FKVEGTLMPPDGPESWPKNISRHQWLVFYRINGMSLEGSGLIDGRGEKWWDLPCKPHRQG 183
++ G + + + ++++ WLVF I +S++G G IDG G WW CK + +
Sbjct: 358 VQISGIIEASENLSDYSEDLTH--WLVFDSIEKLSVKGGGTIDGNGNIWWQNSCKVNEKL 531
Query: 184 PHWTTLPGPC-DSPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKVHVESIYITAPKL 242
P C ++P A+ F+ +LTV+ L +KN Q F V V + +TAP
Sbjct: 532 P--------CKNAPTALTFYKCKDLTVEDLTIKNGQQMQVSFQNSENVQVSDLTVTAPGD 687
Query: 243 SPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSGCYDVDIKNITCGPGHGISIGSLGIHN 302
SPNTDGIH+ NT +++I NS+I GDDC+SI SG DV +I CGPGHGISIGSLG
Sbjct: 688 SPNTDGIHVTNTQNIQISNSVIGTGDDCISIVSGSKDVLATDIICGPGHGISIGSLGAEG 867
Query: 303 SRACVSNITVRDSMMKGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNPIIIDQFYCIT 362
S+ VS ITV+ + + GT NG+RIKTWQGGSGS S ++F NI+M NV NPIIIDQ YC
Sbjct: 868 SKDFVSGITVKGAQLSGTTNGLRIKTWQGGSGSASNIQFQNIQMDNVANPIIIDQNYCDQ 1047
Query: 363 E-GCTNKTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIEL 413
E C +TSAV + N+LY NI GT + ++F CS PC + L +I+L
Sbjct: 1048ETPCEEQTSAVQIRNVLYQNISGT-SASDVGVQFDCSKKFPCQGIVLQNIDL 1200
>BE346184
Length = 458
Score = 223 bits (567), Expect = 2e-58
Identities = 101/151 (66%), Positives = 124/151 (81%)
Frame = +3
Query: 157 MSLEGSGLIDGRGEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKN 216
M+L G+G I+G G+KWWDLPCKPHR GP+ TL GPC SP IRFFMSSNL V+GL+++N
Sbjct: 9 MTLNGTGTIEGNGDKWWDLPCKPHR-GPNGKTLSGPCGSPAMIRFFMSSNLKVKGLKIQN 185
Query: 217 SPQFNFRFDGCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSG 276
SPQF+ F+GC V ++ + I++PKLSPNTDGIH+EN+ V IYNSMISNGDDC+SIG G
Sbjct: 186 SPQFHMIFNGCQGVLIDKLSISSPKLSPNTDGIHVENSKYVGIYNSMISNGDDCISIGPG 365
Query: 277 CYDVDIKNITCGPGHGISIGSLGIHNSRACV 307
+VDI +TCGP HGISIGSLG+HNS+ACV
Sbjct: 366 SSNVDIAGLTCGPSHGISIGSLGVHNSQACV 458
>CA801781
Length = 445
Score = 216 bits (550), Expect = 2e-56
Identities = 94/148 (63%), Positives = 127/148 (85%)
Frame = +1
Query: 297 SLGIHNSRACVSNITVRDSMMKGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNPIIID 356
SLG+HNS+ACVSN+TVRDS+++ +DNG+RIKTWQGG GSVS ++F NI+M NV N IIID
Sbjct: 1 SLGVHNSQACVSNLTVRDSIIRESDNGLRIKTWQGGMGSVSSLRFENIQMENVGNCIIID 180
Query: 357 QFYCITEGCTNKTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPA 416
Q+YC+++ C N+TSAV ++++ Y+NIKGTYDVR+ P+ FACSD++ CT++ LS++ELLP
Sbjct: 181 QYYCMSKECLNQTSAVHLNDVSYSNIKGTYDVRTAPIHFACSDTVACTNITLSEVELLPF 360
Query: 417 EGDIAHDPFCWNAYGDLQTLTIPPVSCL 444
EG + DPFCWNAYG +TLTIPP++CL
Sbjct: 361 EGALLDDPFCWNAYGTQETLTIPPINCL 444
>TC231585 weakly similar to UP|Q9M7D3 (Q9M7D3) Polygalacturonase, partial
(48%)
Length = 878
Score = 197 bits (502), Expect = 6e-51
Identities = 107/237 (45%), Positives = 143/237 (60%), Gaps = 1/237 (0%)
Frame = +2
Query: 209 VQGLRVKNSPQFNFRFDGCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGD 268
V+ L + NS + + F C+++ + + AP SPNTDGIHI T V++ +S+I GD
Sbjct: 20 VRNLMLINSQRMHLSFTNCMRIVASHLKVLAPAFSPNTDGIHISATKGVEVRDSVIRTGD 199
Query: 269 DCVSIGSGCYDVDIKNITCGPGHGISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIKT 328
DC+SI V I+NI+CGPGHGISIGSLG V N+ V + TDNGVRIKT
Sbjct: 200 DCISIVRNSSRVWIRNISCGPGHGISIGSLGKSKKWEKVQNVIVDGVYLYNTDNGVRIKT 379
Query: 329 WQGGSGSVSGVKFSNIRMVNVRNPIIIDQFYCITEG-CTNKTSAVFVSNILYTNIKGTYD 387
WQGGSG S + F +I M NV NPII+DQ+YC + C N+TSAV V NI + +I+GT
Sbjct: 380 WQGGSGFASKITFQHILMENVSNPIIVDQYYCDSRNPCKNETSAVRVENISFIDIQGT-S 556
Query: 388 VRSLPMRFACSDSIPCTHLALSDIELLPAEGDIAHDPFCWNAYGDLQTLTIPPVSCL 444
++F+CSD+ PC L L +I L G FCW A+G + + P +CL
Sbjct: 557 ATEEAIKFSCSDAFPCEGLYLENIFLASCFGGNT-SSFCWQAHGSARGF-LHPSTCL 721
>TC208008 homologue to UP|Q94KI6 (Q94KI6) Polygalacturonase (Fragment),
partial (46%)
Length = 1144
Score = 179 bits (453), Expect = 3e-45
Identities = 89/163 (54%), Positives = 113/163 (68%), Gaps = 4/163 (2%)
Frame = +3
Query: 299 GIHNSRACVSNITVRDSMMKGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNPIIIDQF 358
G N+RACVSNITVRD M T NGVRIKTWQGGSGSV GV FSNI++ V PI+IDQF
Sbjct: 435 GKDNTRACVSNITVRDVNMHNTMNGVRIKTWQGGSGSVQGVLFSNIQVSEVELPIVIDQF 614
Query: 359 YCITEGCTNKTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPAEG 418
YC C N+TSAV ++ I Y I+GTY V+ P+ FACSDS+PC ++L+ +EL P +
Sbjct: 615 YCDKRTCKNQTSAVSLAGINYERIRGTYTVK--PVHFACSDSLPCVDVSLTSVELKPIQE 788
Query: 419 DI-AHDPFCWNAYGDLQTLTIPPVSCLMQGTPQS---WLDHDI 457
++PFCW YG+L+T T+PP+ CL G P + DHD+
Sbjct: 789 KYHLYNPFCWQTYGELKTPTVPPIDCLQIGKPTNNRIQTDHDL 917
>TC212447 weakly similar to UP|O82586 (O82586) Endopolygalacturonase, partial
(54%)
Length = 852
Score = 111 bits (278), Expect(2) = 2e-36
Identities = 67/164 (40%), Positives = 91/164 (54%), Gaps = 2/164 (1%)
Frame = +3
Query: 282 IKNITCGPGHGISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIKTW-QGGSGSVSGVK 340
+ ++ CGPGHGISIGSLG V N+TVR + T NG RIK+W + SG V V
Sbjct: 231 VGDVACGPGHGISIGSLGWDLDEPGVKNVTVRKATFSKTQNGFRIKSWGRPSSGFVEDVH 410
Query: 341 FSNIRMVNVRNPIIIDQFYC-ITEGCTNKTSAVFVSNILYTNIKGTYDVRSLPMRFACSD 399
F + M +V+NPIIIDQ YC GC ++ S V +S+I Y +I GT + ++F CS
Sbjct: 411 FEHATMSDVQNPIIIDQHYCPFRNGCPSQASGVKISDISYKDIHGT-SATQVAVKFDCSS 587
Query: 400 SIPCTHLALSDIELLPAEGDIAHDPFCWNAYGDLQTLTIPPVSC 443
PC + L DI + + A C +A G + + PVSC
Sbjct: 588 EQPCERITLEDIR-FTYKINKAPQALCNHAGGTTLGI-VQPVSC 713
Score = 59.3 bits (142), Expect(2) = 2e-36
Identities = 27/72 (37%), Positives = 40/72 (55%)
Frame = +2
Query: 206 NLTVQGLRVKNSPQFNFRFDGCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMIS 265
++ + GL NS + + C V + + + A SPNTDGIH++ + DV I I
Sbjct: 2 HIVITGLTSLNSQLVHILINACHNVKMHGVKLMADGNSPNTDGIHVKFSTDVTILAPRIR 181
Query: 266 NGDDCVSIGSGC 277
GDDC+S+G GC
Sbjct: 182 TGDDCISVGPGC 217
>TC231081 weakly similar to UP|Q9M7D3 (Q9M7D3) Polygalacturonase, partial
(25%)
Length = 690
Score = 127 bits (318), Expect(2) = 1e-35
Identities = 61/173 (35%), Positives = 99/173 (56%), Gaps = 1/173 (0%)
Frame = +1
Query: 124 FKVEGTLMPPDGPESWPKNISRHQWLVFYRINGMSLEGSGLIDGRGEKWWDLPCKPHRQG 183
F++ GT++ P P W +++ +WL F+ +N ++++G G I+G G++WW CK +
Sbjct: 199 FEISGTIVAPQDPVVW-HGLNQRKWLYFHGVNHLTVDGGGRINGMGQEWWARSCKINSTN 375
Query: 184 PHWTTLPGPCD-SPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKVHVESIYITAPKL 242
P C +P A+ F +L V+ L + +S + + F C+++ + + AP
Sbjct: 376 P--------CHPAPTAMTFHRXQDLXVRHLMLIDSQRMHLSFTNCMRIGAXHLXVLAPAF 531
Query: 243 SPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSGCYDVDIKNITCGPGHGISI 295
SPNTDGIHI T V++ +S I GDDC+ V I+NI+CGPGHGIS+
Sbjct: 532 SPNTDGIHISVTXGVEVMHSXIRTGDDCIXHSRKSSRVWIRNISCGPGHGISM 690
Score = 41.6 bits (96), Expect(2) = 1e-35
Identities = 21/59 (35%), Positives = 33/59 (55%), Gaps = 1/59 (1%)
Frame = +2
Query: 68 FGAVGDGIADDTVSFKMAWDTACQSELPGNV-ILVPKGFSFIIQSTIFTGPCQGGIVFK 125
+GA GDG+ +DT +F AW AC L G + ++ P G +F++ GPC+ I +
Sbjct: 8 YGAKGDGLHNDTEAFLEAWKIAC--SLSGFISVVFPYGKTFLVHPVDIGGPCRSKITLR 178
>TC222699 weakly similar to UP|Q9LW07 (Q9LW07) Polygalacturonase-like
protein, partial (21%)
Length = 721
Score = 122 bits (306), Expect = 3e-28
Identities = 72/211 (34%), Positives = 114/211 (53%), Gaps = 5/211 (2%)
Frame = +1
Query: 63 FDVTTFGAVGDGIADDTVSFKMAWDTACQSELPGNVILVPKGFSFIIQSTIFTGPCQGGI 122
F+V +GA G+G DD+ +F AW AC + +L+PK +F++Q +F GPC+
Sbjct: 127 FNVIDYGATGNGQTDDSQAFLKAWKDACNASYGTATLLIPKEKTFMLQPVLFRGPCKPPT 306
Query: 123 V-FKVEGTLMPPDGPESW--PKNISRHQWLVFYRINGMSLEGS--GLIDGRGEKWWDLPC 177
V K++GT++ P+ E+W PK+ +R W+ F I+G+ + G GLIDG+G WW+
Sbjct: 307 VHIKLKGTIIAPNKIEAWKLPKS-TRMAWIRFRHISGLVIRGGGWGLIDGQGSPWWN--- 474
Query: 178 KPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKVHVESIYI 237
++ T P A+ F L + GL NSP+ + + C + I++
Sbjct: 475 ------SYFNT---EIKRPTALHFRECDYLFLSGLTHINSPKNHISINRCNNSLISKIHM 627
Query: 238 TAPKLSPNTDGIHIENTNDVKIYNSMISNGD 268
AP SPNTDGI I ++++ I NS + GD
Sbjct: 628 IAPDESPNTDGIDISQSSNIVIKNSKMETGD 720
>CA853310
Length = 555
Score = 110 bits (275), Expect(2) = 1e-27
Identities = 59/157 (37%), Positives = 82/157 (51%), Gaps = 1/157 (0%)
Frame = +1
Query: 172 WWDLPCKPHRQGPHWTTLPGP-CDSPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKV 230
WW PC + Q PG C P A+ F + L ++G N + + C K
Sbjct: 88 WWQQPCVGNPQ-------PGATCRPPTAVTFNRCNRLQLKGYTSINPARSHVTLTSCNKG 246
Query: 231 HVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSGCYDVDIKNITCGPG 290
+ +I + AP SPNTDGI I + +++ NS I+ GDDC++I +G + I ITCGPG
Sbjct: 247 IISNIRLIAPGTSPNTDGIDISGSTGIQVLNSFIATGDDCIAISAGSSKIKITGITCGPG 426
Query: 291 HGISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIK 327
HGISIGSLG V ++ V + + T GVR K
Sbjct: 427 HGISIGSLGTRGDTDIVEDVHVENCTLTETLTGVRTK 537
Score = 30.8 bits (68), Expect(2) = 1e-27
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +3
Query: 146 HQWLVFYRINGMSLEGSGLIDGRG 169
H L F +NG+++ G G IDGRG
Sbjct: 9 HMALAFSFVNGLAISGKGTIDGRG 80
>BG650346 similar to GP|6624205|dbj polygalacturonase {Cucumis sativus},
partial (31%)
Length = 414
Score = 114 bits (286), Expect = 7e-26
Identities = 60/144 (41%), Positives = 82/144 (56%), Gaps = 2/144 (1%)
Frame = +3
Query: 169 GEKWWDLPCKPHRQGPHWTTLPGPCD-SPVAIRFFMSSNLTVQGLRVKNSPQ-FNFRFDG 226
G KWW CK ++ P C +P A SS++ V+GL ++N PQ +F
Sbjct: 6 GSKWWAASCKKNKSNP--------CKGAPTAFTIDTSSSIRVKGLTIQNXPQQMHFTISR 161
Query: 227 CIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSGCYDVDIKNIT 286
C V + + ++AP SPNTDGIHI + +V I +S I GDDC+SI + + +K I
Sbjct: 162 CDSVRITGVKVSAPGDSPNTDGIHISESTNVIIQDSKIGTGDDCISIVNASSYIKMKRIY 341
Query: 287 CGPGHGISIGSLGIHNSRACVSNI 310
CGPGHGISIGSLG NS V+ +
Sbjct: 342 CGPGHGISIGSLGKDNSTGIVTKV 413
>TC234738 similar to UP|Q94EH4 (Q94EH4) At1g48100/F21D18_17, partial (15%)
Length = 489
Score = 88.2 bits (217), Expect(2) = 6e-20
Identities = 36/70 (51%), Positives = 51/70 (72%)
Frame = +2
Query: 391 LPMRFACSDSIPCTHLALSDIELLPAEGDIAHDPFCWNAYGDLQTLTIPPVSCLMQGTPQ 450
L + FACSD++ CT++ LS+IELLP EG++ DPFCWNAYG +T+TIPP+ CL +G P
Sbjct: 41 LLIHFACSDTVACTNITLSEIELLPYEGELLDDPFCWNAYGTQETMTIPPLDCLREGEPD 220
Query: 451 SWLDHDINGC 460
+ ++ C
Sbjct: 221 TVVELSAYDC 250
Score = 27.3 bits (59), Expect(2) = 6e-20
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +1
Query: 379 YTNIKGTYDVRSLP 392
Y NIKGTYDVR+ P
Sbjct: 4 YRNIKGTYDVRTPP 45
>TC217817 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (92%)
Length = 1694
Score = 93.2 bits (230), Expect = 2e-19
Identities = 103/420 (24%), Positives = 173/420 (40%), Gaps = 34/420 (8%)
Frame = +1
Query: 65 VTTFGAVGDGIADDTVSFKMAWDTACQ-SELPGNVILVPKG------FSFIIQSTIFTGP 117
+T FG VGDG +T +F+ A Q + G+ + VP G FS T++
Sbjct: 208 LTDFGGVGDGKTSNTKAFQSAISHLSQYASEGGSQLYVPAGKWLTGSFSLTSHFTLYLDK 387
Query: 118 CQGGIVFKVEGTLMPPDGPESWP-------------KNISRHQWLVF-YRINGMSLEG-S 162
+ L+ WP R L+F + + + G +
Sbjct: 388 ---------DAVLLASQDITEWPVLEPLPSYGRGRDAPAGRFTSLIFGTNLTDVIVTGEN 540
Query: 163 GLIDGRGEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFNF 222
G IDG+GE WW + HR+ +T P I S N+ + L + NSP +N
Sbjct: 541 GTIDGQGEFWWQ---QFHRKKLKYTR-------PYLIE*MFSDNIQISNLTLLNSPSWNV 690
Query: 223 RFDGCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSGCYDVDI 282
+ V+ I I AP SPNTDGI+ ++ +V+I + I +GDDCV++ SG + I
Sbjct: 691 HPVYSSNIIVQGITIYAPVTSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGI 870
Query: 283 K-----------NITCGPGHGISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIKTWQG 331
K +TC + +I +LG S + ++ D T++GVRIKT G
Sbjct: 871 KFGWPTKQLVIRRLTCISPYSATI-ALGSEMSGG-IQDVRAEDITAIQTESGVRIKTAVG 1044
Query: 332 GSGSVSGVKFSNIRMVNVRNPI-IIDQFYCITEGCTNKTSAVFVSNILYTNIKGTYDVRS 390
G V + + + ++ + + +G + + + NI Y ++ + +
Sbjct: 1045RGGYVKDIYVKRMTLHTMKWAFKMTGDYNSHADGHYDPNALPEIKNINYRDV--VAENVT 1218
Query: 391 LPMRFACSDSIPCTHLALSDIELLPAEGDIAHDPFCWNAYGDLQTLTIPPVSCLMQGTPQ 450
+ RF + P T + ++++ L A C + G +T PP L P+
Sbjct: 1219IAARFQGISNDPFTGICIANVTLRMAAKAKKQPWTCTDIEGMTSGVTPPPCGLLPDQGPE 1398
>TC217917 similar to UP|Q9M1L0 (Q9M1L0) Polygalacturonase-like protein
(AT3g42950/F18P9_110), partial (74%)
Length = 1292
Score = 85.9 bits (211), Expect = 3e-17
Identities = 74/267 (27%), Positives = 128/267 (47%), Gaps = 15/267 (5%)
Frame = +2
Query: 162 SGLIDGRGEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFN 221
+G I+G+G+ WW K +RQ GP ++ SS++ + + +++SP +
Sbjct: 170 NGTINGQGQTWW----KKYRQ-KRLNHTRGPL-----VQIMFSSDIVITNITLRDSPFWT 319
Query: 222 FRFDGCIKVHVESIYITAPKL-SPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSG---- 276
C + ++ + I AP +PNTDGI ++ D+ I + IS GDD ++I SG
Sbjct: 320 LHPYDCKNITIKGVTILAPVFGAPNTDGIDPDSCEDMLIEDCYISVGDDAIAIKSGWDQY 499
Query: 277 -------CYDVDIKNITCGP--GHGISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIK 327
++ I+N+ GISIGS VSN+TV + ++ + GVRIK
Sbjct: 500 GIAYGRPSMNIMIRNLVVRSMVSAGISIGS----EMSGGVSNVTVENILIWDSRRGVRIK 667
Query: 328 TWQGGSGSVSGVKFSNIRMVNVRNPIIIDQFYC-ITEGCTNKTSAVFVSNILYTNIKGTY 386
T +G V + + NI NVR I++ Y + + + + +I +T + G
Sbjct: 668 TARGRGAYVRQITYRNITFENVRVGIVMKTDYNEHPDDGYDPMALPILRDISFTTVHG-Q 844
Query: 387 DVRSLPMRFACSDSIPCTHLALSDIEL 413
VR +P+R S+ IP ++ D+ +
Sbjct: 845 GVR-VPVRIHGSEEIPVRNVTFQDMSV 922
>TC205977 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (88%)
Length = 1966
Score = 82.4 bits (202), Expect = 4e-16
Identities = 81/316 (25%), Positives = 132/316 (41%), Gaps = 33/316 (10%)
Frame = +3
Query: 68 FGAVGDGIADDTVSFKMAWDTACQ-SELPGNVILVPKG------FSFIIQSTIFTGPCQG 120
FG VGDG +T +F+ A + Q + G+ + VP G F+ T+F
Sbjct: 405 FGGVGDGTTLNTKAFQAAIENLSQYAASGGSQLYVPPGKWLTGSFNLTSHFTLF------ 566
Query: 121 GIVFKVEGTLMPPDGPESWP-------------KNISRHQWLVF-YRINGMSLEG-SGLI 165
+ ++ WP R L+F + + + G +G I
Sbjct: 567 ---LHKDAVILASQDENDWPVIDPLPSYGRGRDTQGGRFSSLIFGTNLTDVIITGDNGTI 737
Query: 166 DGRGEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFNFRFD 225
DG+G+ WW K H+ +T P I S N+ + L + NSP +N
Sbjct: 738 DGQGDLWWQ---KFHKGELKYTR-------PYLIEIMYSDNVQISNLTLVNSPSWNVHPI 887
Query: 226 GCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSG--------- 276
+ V+ I I AP SPNTDGI+ ++ + +I + I +GDDCV++ SG
Sbjct: 888 YSSNLVVQGITILAPVTSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYG 1067
Query: 277 --CYDVDIKNITCGPGHGISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIKTWQGGSG 334
+ I+ +TC I +LG S + ++ D + +++GVRIKT G G
Sbjct: 1068MPTKQLVIRRLTCISPFSAVI-ALGSEMSGG-IQDVRAEDIVAINSESGVRIKTAVGRGG 1241
Query: 335 SVSGVKFSNIRMVNVR 350
V + + M ++
Sbjct: 1242YVKDIFVRRMTMKTMK 1289
>TC218204 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like protein,
partial (51%)
Length = 1014
Score = 80.5 bits (197), Expect = 1e-15
Identities = 75/269 (27%), Positives = 116/269 (42%), Gaps = 13/269 (4%)
Frame = +2
Query: 196 PVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKVHVESIYITAPKLSPNTDGIHIENTN 255
P + F S ++ + + KNSP +N C V V + I AP+ SPNTDGI ++++
Sbjct: 17 PNLVEFVNSQDIIISNVIFKNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSS 196
Query: 256 DVKIYNSMISNGDDCVSIGSG-----------CYDVDIKNIT-CGPGHGISIGSLGIHNS 303
+V I +S IS GDD V++ SG Y + I+ +T P GI+IGS +
Sbjct: 197 NVCIEDSYISTGDDLVAVKSGWDEYGIAYGRPSYGITIRRVTGSSPFAGIAIGS----ET 364
Query: 304 RACVSNITVRDSMMKGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNPI-IIDQFYCIT 362
V N+ + G+ IKT G G + + +++ + N R I I
Sbjct: 365 SGGVENVLAEHINLFNMGVGIHIKTNSGRGGLIKNITVAHVYVENARQGIKIAGDVGGHP 544
Query: 363 EGCTNKTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPAEGDIAH 422
+ N + V I N+ G V + +S P T + LSDI EG +
Sbjct: 545 DEKFNPNALPVVKGITIKNVWGV-KVNQAGLIHGLRNS-PFTDVCLSDINFHGMEGPRSP 718
Query: 423 DPFCWNAYGDLQTLTIPPVSCLMQGTPQS 451
C + +G ++ P S L P S
Sbjct: 719 SWKCSDVFGFAHQVSPWPCSQLSSQEPGS 805
>TC206561
Length = 1637
Score = 75.5 bits (184), Expect = 5e-14
Identities = 82/327 (25%), Positives = 135/327 (41%), Gaps = 28/327 (8%)
Frame = +1
Query: 51 PPPHNCYNATGIFDVTTFGAVGDGIADDTVSFKMAWDTACQ-SELPGNVILVPKG----- 104
P PH+ + FGAVGDG +TV+F+ A A ++ G + VP G
Sbjct: 28 PRPHSV-------SILEFGAVGDGKTLNTVAFQNAVFYAKSFADKGGAKLYVPSGKWLTG 186
Query: 105 -FSFIIQSTIFTGPCQGGIVFKVEGTLMPPDGPESWPKNIS----RHQWLVF-YRINGMS 158
F+ T+F I + D S+ + I R++ L++ ++ +
Sbjct: 187 SFNLTSHLTLFLERGATIIASQDYAHWTAMDPLPSYGRGIDVPVGRYRSLIYGQNLSDVV 366
Query: 159 LEG-SGLIDGRGEKWWDLPCKP--HRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVK 215
+ G +G+IDG+G WWDL + PH L G S N+T+ L
Sbjct: 367 ITGDNGIIDGQGSVWWDLISTHSLNYSRPHIIELVG------------SDNITISNLTFL 510
Query: 216 NSPQFNFRFDGCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGS 275
NSP ++ C V ++ I + AP P T GI +++ V I N IS G D + + S
Sbjct: 511 NSPAWSIHPVYCSNVQIQKITVQAPTKFPYTSGIVPDSSEHVCIDNCNISTGHDAIVLKS 690
Query: 276 G------CYDVDIKNI-------TCGPGHGISIGSLGIHNSRACVSNITVRDSMMKGTDN 322
G Y N+ G G++ GS +S+I + +
Sbjct: 691 GWDEYGVAYGKPTSNVHIRGVYLQSSSGAGLAFGS----EMSGGISDIIAEQLHITNSTF 858
Query: 323 GVRIKTWQGGSGSVSGVKFSNIRMVNV 349
G+ +KT +G G + + S+ ++ N+
Sbjct: 859 GIELKTTRGRGGYMKNIFISDAKLENI 939
>TC218721 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like protein,
partial (53%)
Length = 1128
Score = 61.6 bits (148), Expect(2) = 9e-13
Identities = 46/138 (33%), Positives = 67/138 (48%), Gaps = 12/138 (8%)
Frame = +3
Query: 230 VHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSG-----------CY 278
V V + I AP+ SPNTDGI +++++V I +S IS GDD V++ SG
Sbjct: 228 VVVRYVTILAPRDSPNTDGIDPDSSSNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRPSS 407
Query: 279 DVDIKNIT-CGPGHGISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIKTWQGGSGSVS 337
D+ I+ IT P GI+IGS + V N+ + G+ IKT G G +
Sbjct: 408 DITIRRITGSSPFAGIAIGS----ETSGGVENVLAEHINLYNMGIGIHIKTNTGRGGFIK 575
Query: 338 GVKFSNIRMVNVRNPIII 355
+ S++ M R I I
Sbjct: 576 NITMSHVYMEEARKGIRI 629
Score = 29.6 bits (65), Expect(2) = 9e-13
Identities = 15/60 (25%), Positives = 27/60 (45%)
Frame = +1
Query: 162 SGLIDGRGEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFN 221
+G IDG+G++WW+ W P + F S ++ + + K+SP +N
Sbjct: 31 NGTIDGQGDEWWN----------KWKQRTLQFTRPNLVEFVNSRDIIISNVIFKSSPFWN 180
>TC223574 homologue to UP|Q9SWS2 (Q9SWS2) Polygalacturonase PG2, partial
(23%)
Length = 408
Score = 68.6 bits (166), Expect = 6e-12
Identities = 32/79 (40%), Positives = 51/79 (64%)
Frame = +3
Query: 189 LPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKVHVESIYITAPKLSPNTDG 248
LP + A+RF+ S +TV + ++NS Q + +FD C V V I +++P SPNTDG
Sbjct: 78 LPSTKPTLKALRFYGSDGVTVTCITIQNSQQTHLKFDSCTNVQVSGISVSSPGDSPNTDG 257
Query: 249 IHIENTNDVKIYNSMISNG 267
IH++N+ +V IY+S ++ G
Sbjct: 258 IHLQNSQNVVIYSSTLACG 314
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.138 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,011,598
Number of Sequences: 63676
Number of extensions: 483405
Number of successful extensions: 5230
Number of sequences better than 10.0: 100
Number of HSP's better than 10.0 without gapping: 4438
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4976
length of query: 460
length of database: 12,639,632
effective HSP length: 101
effective length of query: 359
effective length of database: 6,208,356
effective search space: 2228799804
effective search space used: 2228799804
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0122.9