BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0121b.8
(368 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP005379 ORF (334 AA) 209 1e-54
TC233466 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable... 105 1e-27
CF922321 108 4e-24
TC233500 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable... 95 4e-20
CF922798 65 5e-11
TC212179 51 7e-07
BI425208 46 2e-05
TC227932 33 0.27
TC210753 similar to UP|Q9SAF1 (Q9SAF1) F3F19.20 protein (Actin-r... 32 0.35
TC216590 weakly similar to GB|CAA20774.1|3581928|SPCC1906 SPCC19... 28 5.0
BE058644 28 6.6
AW234677 28 6.6
TC206937 similar to UP|Q40161 (Q40161) Polygalacturonase isoenzy... 28 6.6
CA800353 28 8.6
TC233478 similar to UP|Q9LU06 (Q9LU06) Arabidopsis thaliana geno... 28 8.6
>NP005379 ORF (334 AA)
Length = 1002
Score = 209 bits (533), Expect = 1e-54
Identities = 95/199 (47%), Positives = 136/199 (67%), Gaps = 11/199 (5%)
Frame = +1
Query: 179 FKWTNESFDALLGLLKDALPEGEKLPSSHYETKKIVGRLGLKYEKIHACPNDCMLFRNEL 238
+ W+++SF +LL ++ D LP+ LP S+Y+ KKI+ +G++Y+KIHACPNDC+L+R+E
Sbjct: 1 YGWSDKSFTSLLQIVHDLLPQDNTLPKSYYQAKKILCPMGMEYQKIHACPNDCILYRHEF 180
Query: 239 ADKNVNKCIKCGASRWK-----------NDEKKVPAKVLRYFPLKSRLQRLFMSSETSRL 287
+ ++KC +CGASR+K N +K PAKVL Y P+ R +RLF + + ++
Sbjct: 181 EE--MSKCPRCGASRYKVKDDEDCSSDENSKKGPPAKVLWYLPIIPRFKRLFANEDDAKD 354
Query: 288 MRWHDKGRNKDGDLRHPADSEAWKKFDDQHQEFAKDSRNVRLGLASDGFNPYSSMKVFDS 347
+ WH GR DG +RHPAD WKK D + F K++RN+RLGLASDG NPY ++ S
Sbjct: 355 LTWHANGRKSDGMVRHPADCSQWKKIDSLYPNFGKEARNLRLGLASDGMNPYGNLSTQHS 534
Query: 348 TWPVILVPYNLPPWLCMKQ 366
+WPV+LV YN PPWLCMK+
Sbjct: 535 SWPVLLVIYNFPPWLCMKR 591
>TC233466 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable element
(Fragment), partial (37%)
Length = 790
Score = 105 bits (261), Expect(2) = 1e-27
Identities = 56/149 (37%), Positives = 91/149 (60%), Gaps = 12/149 (8%)
Frame = +2
Query: 158 SGCKKF-SKLSFVLHLYHIKRLFKWTNESFDALLGLLKDALPEGEKLPSSHYETKKIVGR 216
SG ++F + VL L ++K + +++SF +LL ++ D LPE LP S+Y+ KKI+
Sbjct: 350 SGVQQFVDVVVVVLSLVNMKARYGCSDKSFSSLLQVVHDVLPEENTLPKSYYQAKKILCS 529
Query: 217 LGLKYEKIHACPNDCMLFRNELADKNVNKCIKCGASRWK-----------NDEKKVPAKV 265
+ ++Y KIHACPNDC+L+R+E + ++KC +CG SR+K N +K AKV
Sbjct: 530 MDMEY*KIHACPNDCILYRHEF--EEMSKCPRCGVSRYKVKDDEECSSDENSKKGPLAKV 703
Query: 266 LRYFPLKSRLQRLFMSSETSRLMRWHDKG 294
L Y P+ + +RLF + + ++ + WH G
Sbjct: 704 LWYLPIVPKFKRLFANGDDAKDLTWHANG 790
Score = 36.2 bits (82), Expect(2) = 1e-27
Identities = 37/154 (24%), Positives = 58/154 (37%), Gaps = 4/154 (2%)
Frame = +3
Query: 17 DGVESFLDHAFRES----NGETIPCPCKKCVLHYVVNRAMAYEHLMVNGIIPSYDTWFCH 72
+GVE FL A S NG+ CPC C+ +HL+ + I +Y TW H
Sbjct: 9 EGVEQFLQLASERSRPDENGKFF-CPCINCLNGRRQKVNDIRDHLLCDEIKRNYTTWIWH 185
Query: 73 GESINVLNRKEETNCREEAFRGDDMVGLIHDAFEGFNQSMGNNISDGGDIESNLDENLTH 132
GE ++ + + E F+ M + + D + +L
Sbjct: 186 GEMADMQSGPQS---------------------EPFDVEMRDRLED-------MIRDLGQ 281
Query: 133 TSRNQSHPEVEKFEQLMKDANEELYSGCKKFSKL 166
S Q+H + ++ L D+ LY GC L
Sbjct: 282 ESFQQAHAPM--YDTLQIDSKTPLYPGCNNLLTL 377
>CF922321
Length = 742
Score = 108 bits (270), Expect = 4e-24
Identities = 49/93 (52%), Positives = 64/93 (68%)
Frame = -1
Query: 274 RLQRLFMSSETSRLMRWHDKGRNKDGDLRHPADSEAWKKFDDQHQEFAKDSRNVRLGLAS 333
RL+RLF + + ++ + WH GRN DG +R A S WKK D + + K++ N+RLGLAS
Sbjct: 733 RLKRLFANGDDAKDLTWHANGRNCDGMIRRLAVSSQWKKIDRLYLDLGKEASNLRLGLAS 554
Query: 334 DGFNPYSSMKVFDSTWPVILVPYNLPPWLCMKQ 366
DG NPY S S+WPV+LV YNLPPWLCMK+
Sbjct: 553 DGMNPYGSSST*HSSWPVLLVIYNLPPWLCMKR 455
>TC233500 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable element
(Fragment), partial (65%)
Length = 1033
Score = 95.1 bits (235), Expect = 4e-20
Identities = 41/79 (51%), Positives = 54/79 (67%)
Frame = +2
Query: 288 MRWHDKGRNKDGDLRHPADSEAWKKFDDQHQEFAKDSRNVRLGLASDGFNPYSSMKVFDS 347
+ WH GRN D LRH ADS WKK D + +F K+++N+ LGLA+D NPY + +
Sbjct: 8 LTWHANGRNCDRMLRHLADSLQWKKIDRLYPDFGKETKNLTLGLATDAMNPYGCLSTQHN 187
Query: 348 TWPVILVPYNLPPWLCMKQ 366
+WPV+LV YNLP WLCMK+
Sbjct: 188 SWPVLLVIYNLPHWLCMKR 244
>CF922798
Length = 617
Score = 65.1 bits (157), Expect = 5e-11
Identities = 28/53 (52%), Positives = 38/53 (70%)
Frame = -2
Query: 314 DDQHQEFAKDSRNVRLGLASDGFNPYSSMKVFDSTWPVILVPYNLPPWLCMKQ 366
D + +F+K++ N+RLGLA+DG NPY S+ S W V+LV YNL PWLCM +
Sbjct: 613 DHLYLDFSKEAINLRLGLATDGMNPYGSLSTQYSLWSVLLVIYNLSPWLCMNK 455
>TC212179
Length = 764
Score = 51.2 bits (121), Expect = 7e-07
Identities = 28/75 (37%), Positives = 37/75 (49%), Gaps = 3/75 (4%)
Frame = +1
Query: 3 RMWMDYDRVSKEYLDGVESFLDHAFRESNGE---TIPCPCKKCVLHYVVNRAMAYEHLMV 59
R WM+ R+S EY +GVE FL A + + CPC C+ EHL+
Sbjct: 37 RSWMNQSRISPEYEEGVEQFLQFASQRGQPDEDGKYYCPCINCLNGRRQILDDIREHLLC 216
Query: 60 NGIIPSYDTWFCHGE 74
+GI +Y TW HGE
Sbjct: 217 DGIKRNYTTWIWHGE 261
>BI425208
Length = 413
Score = 46.2 bits (108), Expect = 2e-05
Identities = 25/67 (37%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Frame = -3
Query: 10 RVSKEYLDGVESFLDHAFRES-NGETIPCPCKKCVLHYVVNRAMAYEHLMVNGIIPSYDT 68
+ S EYL G+ FL+ +F S N TI CPC +C Y R L+ + +Y+
Sbjct: 201 QASIEYLKGINDFLEFSFSYSKNKNTIRCPCCRCNAIYYKTRDEVIGDLLKSRFQQNYEH 22
Query: 69 WFCHGES 75
W HGES
Sbjct: 21 WESHGES 1
>TC227932
Length = 1562
Score = 32.7 bits (73), Expect = 0.27
Identities = 42/157 (26%), Positives = 68/157 (42%), Gaps = 11/157 (7%)
Frame = +1
Query: 121 DIESNLDENLTHTSRNQSHPEV----EKFEQLMKDANEELYSGCKKFSKLSFVLHLYHIK 176
D+ S L H+S + +PE+ E+ + + EE G SKL+ +H K
Sbjct: 802 DLSSELLTFQLHSSVHPINPELFLVLEQTKASSLLSKEESIPGKDANSKLN--KEKFHRK 975
Query: 177 RLFKWTNESFDALLGLLKDALPEGEKLPSSHYETKK------IVGRLGLKYEKIHACPND 230
+F NE +LG + PE P+S+ TKK ++ L + EKI A +
Sbjct: 976 LIFDSVNE----ILGAKFSSSPEPWIQPNSNRLTKKTLSAQKLLKELCFEIEKIQAKKTE 1143
Query: 231 CMLFRNELADKNV-NKCIKCGASRWKNDEKKVPAKVL 266
C L + KN+ + + G+ W + +P VL
Sbjct: 1144CSLEEEDDGLKNILCEDVLHGSESWTDFHGYLPGVVL 1254
>TC210753 similar to UP|Q9SAF1 (Q9SAF1) F3F19.20 protein (Actin-related
protein 3) (At1g13180), partial (8%)
Length = 1210
Score = 32.3 bits (72), Expect = 0.35
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +3
Query: 56 HLMVNGIIPSYDTWFCHGESINV 78
HL+ +GI+P+Y W HGES N+
Sbjct: 309 HLICDGIVPTYIKWI*HGESSNM 377
>TC216590 weakly similar to GB|CAA20774.1|3581928|SPCC1906 SPCC1906.05
{Schizosaccharomyces pombe;} , partial (7%)
Length = 1758
Score = 28.5 bits (62), Expect = 5.0
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = +1
Query: 117 SDGGDIESNLDENLTHTSRNQSHPEVEKFEQLMKDANEELYSGCKKFSKLS 167
+D + ++D+NL S + +PEVE+ K + L SGC K K++
Sbjct: 991 TDVSSEKKHVDQNLKELSASSENPEVEE-----KQEDLSLPSGCSKDDKVT 1128
>BE058644
Length = 425
Score = 28.1 bits (61), Expect = 6.6
Identities = 19/53 (35%), Positives = 27/53 (50%)
Frame = +3
Query: 246 CIKCGASRWKNDEKKVPAKVLRYFPLKSRLQRLFMSSETSRLMRWHDKGRNKD 298
C+ G S DE KV + L S + MS+ETSRL+ ++ +NKD
Sbjct: 39 CVFIGISLLAPDESKVSGPETKDSSLDSMVSSA-MSTETSRLVVSPEEAQNKD 194
>AW234677
Length = 428
Score = 28.1 bits (61), Expect = 6.6
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +3
Query: 164 SKLSFVLHLYHIKRLFKWTNESFDAL 189
S LS+ HL ++ +F W ESF AL
Sbjct: 51 SSLSYASHLGYLHLMFGWVPESFSAL 128
>TC206937 similar to UP|Q40161 (Q40161) Polygalacturonase isoenzyme 1 beta
subunit precursor , partial (23%)
Length = 537
Score = 28.1 bits (61), Expect = 6.6
Identities = 17/43 (39%), Positives = 29/43 (66%)
Frame = +2
Query: 175 IKRLFKWTNESFDALLGLLKDALPEGEKLPSSHYETKKIVGRL 217
+K++FK ++ ++ ++KD+L E E+ PSS ETK+ VG L
Sbjct: 20 LKQVFKASDNG--SMEKMMKDSLEECERAPSSG-ETKRCVGSL 139
>CA800353
Length = 325
Score = 27.7 bits (60), Expect = 8.6
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -2
Query: 297 KDGDLRHPADSEAWKKFDDQHQEFAKDSRN 326
K+G+ P SEAW + + H E+ D R+
Sbjct: 252 KEGNGHEPLQSEAWFRCGEAHAEYHGDERD 163
>TC233478 similar to UP|Q9LU06 (Q9LU06) Arabidopsis thaliana genomic DNA,
chromosome 5, TAC clone:K15C23, partial (37%)
Length = 704
Score = 27.7 bits (60), Expect = 8.6
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 30 SNGETIPCPCKKCVLHYVVNRAMAY 54
S ++I PCK+C HY +N+ A+
Sbjct: 400 SKNQSIFVPCKECCEHYKMNQGAAF 326
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.136 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,411,333
Number of Sequences: 63676
Number of extensions: 255296
Number of successful extensions: 1368
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 1360
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1363
length of query: 368
length of database: 12,639,632
effective HSP length: 99
effective length of query: 269
effective length of database: 6,335,708
effective search space: 1704305452
effective search space used: 1704305452
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0121b.8