BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0113.5
(992 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA851030 50 4e-06
CA936755 45 2e-04
CO982668 44 5e-04
TC234374 43 6e-04
TC206673 43 8e-04
BG405621 42 0.002
TC223473 41 0.003
TC213164 39 0.015
BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase... 38 0.019
CA938853 similar to GP|1684847|gb|A pinin {Homo sapiens}, partia... 35 0.13
TC233199 34 0.28
BI968991 34 0.28
TC216024 similar to GB|AAM78036.1|21928015|AY125526 At2g01100/F2... 34 0.28
TC219587 similar to PIR|T49181|T49181 cyclophylin-like protein -... 34 0.37
TC204478 similar to UP|ARFB_ARATH (Q94JM3) Auxin response factor... 33 0.62
CA937241 similar to GP|6840918|gb|A hematopoietic antigen CD38 {... 33 0.62
TC214589 weakly similar to UP|K502_ACTCH (P43394) Fruit protein ... 33 0.82
TC231484 similar to UP|Q6ZLD4 (Q6ZLD4) Cyclophylin-like protein,... 32 1.4
TC221817 similar to GB|AAP37839.1|30725634|BT008480 At5g64750 {A... 32 1.8
BE661094 30 4.0
>CA851030
Length = 358
Score = 50.4 bits (119), Expect = 4e-06
Identities = 21/64 (32%), Positives = 35/64 (53%)
Frame = +1
Query: 835 TVLIREELLWRQKSRVNWLNHGDRNTHFFHTTTMVHRKRNKIEALNNDVGELITDQDQLR 894
++L +EE W+Q+SR W D+NT FFH T+ + N I + +D G + DQ+
Sbjct: 157 SLLKQEEEYWQQRSRAIWFKERDKNTEFFHRKTLQRKASNSITKIQDDEGNVCFKPDQIE 336
Query: 895 SMAV 898
+ +
Sbjct: 337 DILI 348
>CA936755
Length = 424
Score = 45.1 bits (105), Expect = 2e-04
Identities = 21/66 (31%), Positives = 40/66 (59%)
Frame = -2
Query: 704 LGNARWISSFPEATVLHLPKLKYDHKPILIQLGPEHMDSSQRPFRFLAAWLTHEDFPRLV 763
L + +W+ S+P+++ LP+ DH PI++Q + +D +PFR + WL + + RLV
Sbjct: 291 LVSEQWLLSWPDSSQYTLPRDFSDHCPIIMQT--KKVDWGPKPFRVVDWWLHQKGYQRLV 118
Query: 764 KSSWDS 769
+ +W +
Sbjct: 117 RETWSA 100
>CO982668
Length = 870
Score = 43.5 bits (101), Expect = 5e-04
Identities = 25/70 (35%), Positives = 35/70 (49%), Gaps = 1/70 (1%)
Frame = -2
Query: 699 RIDWALGNARWISSFPEATVLHLPKLKYDHKPILIQLGPE-HMDSSQRPFRFLAAWLTHE 757
R+D L N W P V HL K D P I L M+ + P+RF AWL+H+
Sbjct: 263 RLDRVLMNQEWRIESPIVKVTHLFHCK*DQ*PHHINLDVG*PMNRKRCPYRFQNAWLSHD 84
Query: 758 DFPRLVKSSW 767
+F L++ +W
Sbjct: 83 EFDSLIRHNW 54
>TC234374
Length = 787
Score = 43.1 bits (100), Expect = 6e-04
Identities = 25/78 (32%), Positives = 41/78 (52%), Gaps = 1/78 (1%)
Frame = +2
Query: 787 SWNRDVFGEIGRTKRRLMRRLEGINNHL-RMSHSPYLEKLQKRLWKEYQTVLIREELLWR 845
+W ++VFG + + ++ I + + +S L + KE QTVL +E LWR
Sbjct: 113 TWIKNVFGNVHQLVL*ATNEVDSIQQQIVNVGYSDALFNQEVLAQKELQTVLRFQECLWR 292
Query: 846 QKSRVNWLNHGDRNTHFF 863
+KSR+ G++NT FF
Sbjct: 293 EKSRI*GHAQGEQNTSFF 346
>TC206673
Length = 742
Score = 42.7 bits (99), Expect = 8e-04
Identities = 23/69 (33%), Positives = 34/69 (48%)
Frame = +3
Query: 811 NNHLRMSHSPYLEKLQKRLWKEYQTVLIREELLWRQKSRVNWLNHGDRNTHFFHTTTMVH 870
N L + ++LQ++LW+ E L RQKSR WL GD N+ +FHT
Sbjct: 69 NRSLSVDEIKAKKELQQQLWEASTAY----ESLLRQKSRDKWLKEGDNNSAYFHTVINFR 236
Query: 871 RKRNKIEAL 879
R N ++ +
Sbjct: 237 RHYNGLQGI 263
>BG405621
Length = 402
Score = 41.6 bits (96), Expect = 0.002
Identities = 31/124 (25%), Positives = 51/124 (41%), Gaps = 3/124 (2%)
Frame = +2
Query: 745 RPFRFLAAWLTHEDFPRLVKSSWDSHHDSWIPASEAFCEEAASWNRDVFGEIGRTKRRLM 804
R F F +WL +V W + + S+ C A G I KR+
Sbjct: 38 RNFIFENSWLLEPGLEEIVHFGWSKPLSNNL-ISKLNC*IVALMK*TNEGRI--LKRKFH 208
Query: 805 RRLEGINNHLRMS---HSPYLEKLQKRLWKEYQTVLIREELLWRQKSRVNWLNHGDRNTH 861
++E + S H P + + + +EE W+Q+S++ WL +GD N+
Sbjct: 209 DQIEACRKDIEASRFFHDPVNDARY*EAQAKLNNFIAQEESYWQQRSKIFWLKYGDLNSK 388
Query: 862 FFHT 865
FFH+
Sbjct: 389 FFHS 400
>TC223473
Length = 654
Score = 40.8 bits (94), Expect = 0.003
Identities = 23/65 (35%), Positives = 29/65 (44%)
Frame = +1
Query: 825 LQKRLWKEYQTVLIREELLWRQKSRVNWLNHGDRNTHFFHTTTMVHRKRNKIEALNNDVG 884
LQ LW+E L +EE LW K W+ G+R R+ N I + N G
Sbjct: 298 LQAELWEELHNTLFQEEFLWF*KFHCRWMKFGNR------------RQLNFIHKIQNSSG 441
Query: 885 ELITD 889
E ITD
Sbjct: 442 EWITD 456
>TC213164
Length = 446
Score = 38.5 bits (88), Expect = 0.015
Identities = 16/56 (28%), Positives = 29/56 (51%)
Frame = +3
Query: 929 DEIMGPLEAAEIRVAVFSMGAMKAPGPDDLNPLFFQSQWQVIGPSVVKLAMTVYSN 984
D ++ E E+R ++ G K+PGPD LN F + W ++ +++ Y+N
Sbjct: 12 DLLVARFEKKEVRATMWDRGNAKSPGPDGLNFKFIKEFWDILKTDLLRFLDEFYAN 179
>BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (15%)
Length = 376
Score = 38.1 bits (87), Expect = 0.019
Identities = 15/45 (33%), Positives = 28/45 (61%)
Frame = -2
Query: 940 IRVAVFSMGAMKAPGPDDLNPLFFQSQWQVIGPSVVKLAMTVYSN 984
I+ AV+ G++K+PGPD LN F + W+ + P +++ ++N
Sbjct: 375 IKSAVWQCGSVKSPGPDGLNFKFIKHFWERLKPDIIRFLNEFHAN 241
>CA938853 similar to GP|1684847|gb|A pinin {Homo sapiens}, partial (2%)
Length = 422
Score = 35.4 bits (80), Expect = 0.13
Identities = 17/46 (36%), Positives = 24/46 (51%)
Frame = -3
Query: 614 VYGSPNASIRNFLWQELQNIASQVTKPWVTVGDFNTYLQASDKMGG 659
VY S R LW EL ++ + W +GDFN+ + A +K GG
Sbjct: 165 VYASTAYVKRRDLWSELTDLQQKHLGSWPFIGDFNSNVGAQEKRGG 28
>TC233199
Length = 1105
Score = 34.3 bits (77), Expect = 0.28
Identities = 22/100 (22%), Positives = 42/100 (42%), Gaps = 3/100 (3%)
Frame = +2
Query: 595 FVHLRIHPKNNVPNFLVTFVYGSPNASIRNFLWQELQNIASQV-TKPWVTVGDFNTYLQA 653
F+ L K + + +Y + +++ LW ++ + S K W V DFN +
Sbjct: 149 FISLEGTWKGDGEKITIVNIYSPCDVNLKRNLWGQINQLRSAS*NKLWCIVDDFNCVRRQ 328
Query: 654 SDKMGGGPPNLLS--MAKFRDCLDACSLSDLGFKGPPFTW 691
S+++G M +F D + + D+ G +TW
Sbjct: 329 SERVGASQREQGGGIMTEFNDWIVDLEVEDMPSVGHKYTW 448
>BI968991
Length = 750
Score = 34.3 bits (77), Expect = 0.28
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +1
Query: 839 REELLWRQKSRVNWLNHGDRNTHFFHTTTMVH 870
+ ++LW +KS W+ GD T +FH T+V+
Sbjct: 13 KNDILWFRKS*AKWIKDGDHETGYFHGLTLVN 108
>TC216024 similar to GB|AAM78036.1|21928015|AY125526 At2g01100/F23H14.7
{Arabidopsis thaliana;} , partial (14%)
Length = 1433
Score = 34.3 bits (77), Expect = 0.28
Identities = 18/57 (31%), Positives = 24/57 (41%)
Frame = +2
Query: 421 SHFKSGQHDKLGPTSGSSSLKKTKMKARGHQRVEGSSEPRRSPNASSNAPGDMGGRT 477
SH+ SG H+K S KK+ + + E SSE R S D G R+
Sbjct: 431 SHYDSGDHEKRKEKSSKRKTKKSSSETSDYDDFESSSEEERRKKKQSKKLRDRGSRS 601
>TC219587 similar to PIR|T49181|T49181 cyclophylin-like protein - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (19%)
Length = 1344
Score = 33.9 bits (76), Expect = 0.37
Identities = 58/273 (21%), Positives = 100/273 (36%), Gaps = 47/273 (17%)
Frame = +1
Query: 276 QTKHSGGKGA--ETRFSILEQMDVDAIHGSSGIGASSSGILA*VQIPEQNNLNQNTPTNQ 333
+++HS + A ++R S + D D+ GSS + + + N +N T +
Sbjct: 73 RSQHSRSRRARRKSRRSSEDSSDTDSASGSSSGDEKTDRHASGRKTLADNEARRNLDTGK 252
Query: 334 ESQ---------PQHMVD----------SAGSG-ISPTKGKVVNTND-----EPNHEGIS 368
+S P+ +D S G +SP G VN P +
Sbjct: 253 QSSSPPQQRQTIPEQKMDPKVRRIVDKQSHEEGELSPENGAFVNNRGASPRRSPTRDSRE 432
Query: 369 SVPNDETMISGPTKITSAPTGPQPHDGGTGTKVNPNSQGAIPQQSRADV---AHPSHFKS 425
++++ P + S P P+ H G +PN ++ R A +++
Sbjct: 433 LNQGHASLLASPGQKVSEPAAPK-HGQGFSKSPSPNGMPKRIKKGRGFTERYAFARRYRT 609
Query: 426 GQHDKLGPTS--GSSSLKKTKMKARGHQRVEGSSEPRR--SPNASSNAPGDMGGRT---- 477
++ T G ++++ + ++ G S PRR SP N P R+
Sbjct: 610 PSPERSPRTYRYGDRNIRRNFDRNTSYRSYSGRSPPRRFRSPPGGRNRPRYQSRRSRSRS 789
Query: 478 ---------FRERDRSLSPRKHRSS*RAFPSIS 501
F +RDRS SPR+ S P IS
Sbjct: 790 NSRSPVRGHFSDRDRSRSPRRSPSPEDRRPPIS 888
>TC204478 similar to UP|ARFB_ARATH (Q94JM3) Auxin response factor 2
(ARF1-binding protein) (ARF1-BP), partial (37%)
Length = 1975
Score = 33.1 bits (74), Expect = 0.62
Identities = 48/198 (24%), Positives = 79/198 (39%), Gaps = 9/198 (4%)
Frame = +3
Query: 705 GNARWISSFPEATVLHLPKLKYDHKPILIQLGPEHMDSSQRPFRFLAAWLTHEDFPRLVK 764
GN R+ S+F E VLH K+++ H+ L+ P S R L+ ++ + P V
Sbjct: 948 GNLRYSSAFGEYPVLHGHKVEHSHRSFLMPPPPSTQYESPRSRELLSKPISGK--PCEVS 1121
Query: 765 SSWDSHHDSW---IPASEAFCEEAASWNRDVFGEIGRTKRRLMRRLEGINNHLRMSHSPY 821
DS + + +S E + R+V E I N + HS
Sbjct: 1122 KLKDSDCKLFGISLLSSRPIASEPSLSQRNVTSESVGHMHTASHHQRAIENDQKSEHSRG 1301
Query: 822 LEKLQKRLWKEYQTVLIREELLWRQKSRVNWLN-HGDRNTHFFHTTTMVHRK-----RNK 875
+ L +++ VL Q S+ + + ++ + T VH+K R+
Sbjct: 1302 SKPADGLLIDDHEKVL--------QTSQPHLKDVQAKSHSGSARSCTKVHKKGIALGRSV 1457
Query: 876 IEALNNDVGELITDQDQL 893
+D GELIT+ DQL
Sbjct: 1458 DLTKFSDYGELITELDQL 1511
>CA937241 similar to GP|6840918|gb|A hematopoietic antigen CD38 {Canis
familiaris}, partial (4%)
Length = 203
Score = 33.1 bits (74), Expect = 0.62
Identities = 12/44 (27%), Positives = 25/44 (56%)
Frame = +2
Query: 862 FFHTTTMVHRKRNKIEALNNDVGELITDQDQLRSMAVTSFQNLY 905
FFH++ + N I +L D ++ +Q ++ + ++ FQNL+
Sbjct: 5 FFHSSAKARKSANNISSLTCDDSAIVHNQKEMSNTTISCFQNLF 136
>TC214589 weakly similar to UP|K502_ACTCH (P43394) Fruit protein PKIWI502,
partial (49%)
Length = 1254
Score = 32.7 bits (73), Expect = 0.82
Identities = 19/78 (24%), Positives = 31/78 (39%)
Frame = +3
Query: 380 PTKITSAPTGPQPHDGGTGTKVNPNSQGAIPQQSRADVAHPSHFKSGQHDKLGPTSGSSS 439
P + P+GP+P + T +P+S +R P+H PT S
Sbjct: 168 PHYARTPPSGPRPLSPRSSTPPSPSSTSPSTSPTRPITPRPTHSPGSTSSSASPTRSSPR 347
Query: 440 LKKTKMKARGHQRVEGSS 457
++ + +RVE SS
Sbjct: 348 FWPSRRRRSSPRRVECSS 401
>TC231484 similar to UP|Q6ZLD4 (Q6ZLD4) Cyclophylin-like protein, partial
(35%)
Length = 884
Score = 32.0 bits (71), Expect = 1.4
Identities = 25/81 (30%), Positives = 34/81 (41%), Gaps = 15/81 (18%)
Frame = +2
Query: 436 GSSSLKKTKMKARGHQRVEGSSEPRR--SPNASSNAPGDMGGRT-------------FRE 480
G ++++ + ++ G S PRR SP N P R+ FRE
Sbjct: 131 GDRNIRRNFDRNTSYRSYSGRSPPRRFRSPPGGRNRPRYQSRRSRSRSISGSPVRGRFRE 310
Query: 481 RDRSLSPRKHRSS*RAFPSIS 501
RDRS SPR+ S P IS
Sbjct: 311 RDRSRSPRRSPSPEDRLPLIS 373
>TC221817 similar to GB|AAP37839.1|30725634|BT008480 At5g64750 {Arabidopsis
thaliana;} , partial (24%)
Length = 1008
Score = 31.6 bits (70), Expect = 1.8
Identities = 35/125 (28%), Positives = 46/125 (36%), Gaps = 11/125 (8%)
Frame = +1
Query: 366 GISSVPNDETMISGPTKITSA--PTGP-------QPHDGGTGTKVNPNSQGAIPQQSRAD 416
GI+ M+S T + S PTG QPHD T V PN P S
Sbjct: 148 GINREREMSAMVSALTHVVSGEVPTGDSSSVLHHQPHDDDAVT-VKPNMPSTTPFSSTYV 324
Query: 417 VAHPSHFKSGQHDKLGPTSG--SSSLKKTKMKARGHQRVEGSSEPRRSPNASSNAPGDMG 474
VA S +S QHD P+ SS A + G++ + N + G
Sbjct: 325 VATSSLKRSRQHDTYTPSFSHPPSSFPAMPTSAECSKSGMGNAVYEYRTRGTENVKKEEG 504
Query: 475 GRTFR 479
R +R
Sbjct: 505 RRKYR 519
>BE661094
Length = 583
Score = 30.4 bits (67), Expect = 4.0
Identities = 17/61 (27%), Positives = 24/61 (38%)
Frame = +1
Query: 320 PEQNNLNQNTPTNQESQPQHMVDSAGSGISPTKGKVVNTNDEPNHEGISSVPNDETMISG 379
PEQ Q E +PQH + P +G + N E + ++ P T SG
Sbjct: 145 PEQTQKGQXXLVLTEHEPQHRLPRT---TKPPRGMFQSQNQEATYSKTTTTPTPTTTXSG 315
Query: 380 P 380
P
Sbjct: 316 P 318
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.328 0.141 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,658,639
Number of Sequences: 63676
Number of extensions: 796390
Number of successful extensions: 4968
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 4910
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4962
length of query: 992
length of database: 12,639,632
effective HSP length: 106
effective length of query: 886
effective length of database: 5,889,976
effective search space: 5218518736
effective search space used: 5218518736
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0113.5