BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0112.9
(1105 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP005379 ORF (334 AA) 262 5e-70
CF922321 192 7e-49
TC233500 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable... 158 1e-38
CF922798 158 1e-38
TC233466 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable... 68 2e-11
BE440417 40 0.004
TC229565 similar to PIR|T05694|T05694 pathogenesis-related prote... 30 4.5
TC232917 30 4.5
AW733327 30 4.5
>NP005379 ORF (334 AA)
Length = 1002
Score = 262 bits (670), Expect = 5e-70
Identities = 129/325 (39%), Positives = 184/325 (55%), Gaps = 8/325 (2%)
Frame = +1
Query: 185 VKFMSDALPNGNCMPKNLYHAKKTVAELGLACVKIDCCPGGCMLYYKEDEMLQSCSP--E 242
++ + D LP N +PK+ Y AKK + +G+ KI CP C+LY E E + C
Sbjct: 34 LQIVHDLLPQDNTLPKSYYQAKKILCPMGMEYQKIHACPNDCILYRHEFEEMSKCPRCGA 213
Query: 243 KRYKEV------RKQGKEKFVPQQKMWYFPLVPRLQRLYSSMQTASHMRWHHEHYKEPDF 296
RYK + +K P + +WY P++PR +RL+++ A + WH K
Sbjct: 214 SRYKVKDDEDCSSDENSKKGPPAKVLWYLPIIPRFKRLFANEDDAKDLTWHANGRKSDGM 393
Query: 297 LSHPSDAEAWMKFDEAWGTFADEPRNVRLGLCSDGFAPFDISGKKYSCWPVIVTPYNLPP 356
+ HP+D W K D + F E RN+RLGL SDG P+ ++S WPV++ YN PP
Sbjct: 394 VRHPADCSQWKKIDSLYPNFGKEARNLRLGLASDGMNPYGNLSTQHSSWPVLLVIYNFPP 573
Query: 357 WMCMRREFMFLTIMIPGPSAPKKSIDVYLRPLIDELKMLWEDGVMTYDVSLKQNFLMKAA 416
W+CM+R++M L++MI GP P IDVYL PLI++L+ LW++GV+ +D K+ F M+A
Sbjct: 574 WLCMKRKYMMLSMMISGPRQPGNDIDVYLSPLIEDLRKLWDEGVLVFDGFRKETFQMRAM 753
Query: 417 VMWTINDFPAYGMLSGWMTMGKLACPVCMERSKAFSLAYSRKVSWFDCHRQFLSEDHEYR 476
+ TINDFPAYG LSG+ G LACP+C E + L + RK + + HR FL H YR
Sbjct: 754 LFCTINDFPAYGNLSGYSVKGHLACPICEEDTSYIQLKHGRK-TVYTRHRVFLKAHHPYR 930
Query: 477 KCPKKFTKGKGETSTKPRRLNGEEV 501
+ K F G E + L GE+V
Sbjct: 931 RLKKAF-NGSQEHEIRRTPLTGEQV 1002
>CF922321
Length = 742
Score = 192 bits (488), Expect = 7e-49
Identities = 93/242 (38%), Positives = 141/242 (57%)
Frame = -1
Query: 267 LVPRLQRLYSSMQTASHMRWHHEHYKEPDFLSHPSDAEAWMKFDEAWGTFADEPRNVRLG 326
++PRL+RL+++ A + WH + + + W K D + E N+RLG
Sbjct: 742 IIPRLKRLFANGDDAKDLTWHANGRNCDGMIRRLAVSSQWKKIDRLYLDLGKEASNLRLG 563
Query: 327 LCSDGFAPFDISGKKYSCWPVIVTPYNLPPWMCMRREFMFLTIMIPGPSAPKKSIDVYLR 386
L SDG P+ S +S WPV++ YNLPPW+CM+R++M L++MI P P IDVYL
Sbjct: 562 LASDGMNPYGSSST*HSSWPVLLVIYNLPPWLCMKRKYMMLSMMISNPRQPGNDIDVYLS 383
Query: 387 PLIDELKMLWEDGVMTYDVSLKQNFLMKAAVMWTINDFPAYGMLSGWMTMGKLACPVCME 446
LI++L LW++G + ++ + F M+A + TINDFPAYG LSG+ G +CP+C E
Sbjct: 382 SLIEDLTKLWDEGFLAFNGFWNETFPMRAMLFCTINDFPAYGNLSGYNVKGHHSCPICEE 203
Query: 447 RSKAFSLAYSRKVSWFDCHRQFLSEDHEYRKCPKKFTKGKGETSTKPRRLNGEEVWERIK 506
+ L + RK + + HR+FL+ +H YR+ K F +G P L EEV++R++
Sbjct: 202 NTSYIQLKHGRK-TVYSRHRRFLTPNHPYRRLKKAFNGSQGH-DIVPIPLTAEEVYQRVQ 29
Query: 507 HL 508
HL
Sbjct: 28 HL 23
>TC233500 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable element
(Fragment), partial (65%)
Length = 1033
Score = 158 bits (400), Expect = 1e-38
Identities = 80/221 (36%), Positives = 122/221 (55%)
Frame = +2
Query: 284 MRWHHEHYKEPDFLSHPSDAEAWMKFDEAWGTFADEPRNVRLGLCSDGFAPFDISGKKYS 343
+ WH L H +D+ W K D + F E +N+ LGL +D P+ +++
Sbjct: 8 LTWHANGRNCDRMLRHLADSLQWKKIDRLYPDFGKETKNLTLGLATDAMNPYGCLSTQHN 187
Query: 344 CWPVIVTPYNLPPWMCMRREFMFLTIMIPGPSAPKKSIDVYLRPLIDELKMLWEDGVMTY 403
WPV++ YNLP W+CM+R++M L++MI GP P I+VYL PLI++L L ++GV+ +
Sbjct: 188 SWPVLLVIYNLPHWLCMKRKYMMLSMMITGPRQPGNDINVYLNPLIEDLTKL*DEGVLVF 367
Query: 404 DVSLKQNFLMKAAVMWTINDFPAYGMLSGWMTMGKLACPVCMERSKAFSLAYSRKVSWFD 463
D + F + A + TINDFP Y LSG+ G ACP+C E + + RK + +
Sbjct: 368 DGFRNETFNLCAMLFCTINDFPTYENLSGYSVKGHRACPICEEDTSYIQQKHGRK-TVYT 544
Query: 464 CHRQFLSEDHEYRKCPKKFTKGKGETSTKPRRLNGEEVWER 504
HR+FL H YR+ K F + E L G++V++R
Sbjct: 545 RHRRFLKPHHPYRRLKKAFNE-S*EHENALVLLTGDQVFQR 664
>CF922798
Length = 617
Score = 158 bits (399), Expect = 1e-38
Identities = 80/199 (40%), Positives = 119/199 (59%)
Frame = -2
Query: 310 DEAWGTFADEPRNVRLGLCSDGFAPFDISGKKYSCWPVIVTPYNLPPWMCMRREFMFLTI 369
D + F+ E N+RLGL +DG P+ +YS W V++ YNL PW+CM +++M L++
Sbjct: 613 DHLYLDFSKEAINLRLGLATDGMNPYGSLSTQYSLWSVLLVIYNLSPWLCMNKKYMMLSM 434
Query: 370 MIPGPSAPKKSIDVYLRPLIDELKMLWEDGVMTYDVSLKQNFLMKAAVMWTINDFPAYGM 429
MI P P IDVYL PLI++L ML ++ V+ +D + F + A + TINDFPAYG
Sbjct: 433 MILSPRQPGNDIDVYLSPLIEDLTML*DEEVLVFDGFQNETFHLCAMLFCTINDFPAYGN 254
Query: 430 LSGWMTMGKLACPVCMERSKAFSLAYSRKVSWFDCHRQFLSEDHEYRKCPKKFTKGKGET 489
LSG+ ACP+C E + L + RK + + H++FL H YR+ K+F G E
Sbjct: 253 LSGYSVKSHRACPICEEDTSYIQLKHGRK-TIYTRHQRFLKPHHLYRRLKKEF-NGSQEH 80
Query: 490 STKPRRLNGEEVWERIKHL 508
T P L G+ V+++++HL
Sbjct: 79 ETTPILLVGDHVYQQVQHL 23
>TC233466 similar to UP|Q6I8M7 (Q6I8M7) Soybean Tgm5 transposable element
(Fragment), partial (37%)
Length = 790
Score = 68.2 bits (165), Expect = 2e-11
Identities = 37/111 (33%), Positives = 55/111 (49%), Gaps = 8/111 (7%)
Frame = +2
Query: 185 VKFMSDALPNGNCMPKNLYHAKKTVAELGLACVKIDCCPGGCMLYYKEDEMLQSC--SPE 242
++ + D LP N +PK+ Y AKK + + + KI CP C+LY E E + C
Sbjct: 449 LQVVHDVLPEENTLPKSYYQAKKILCSMDMEY*KIHACPNDCILYRHEFEEMSKCPRCGV 628
Query: 243 KRYK-----EVRKQGKEKFVPQQK-MWYFPLVPRLQRLYSSMQTASHMRWH 287
RYK E K P K +WY P+VP+ +RL+++ A + WH
Sbjct: 629 SRYKVKDDEECSSDENSKKGPLAKVLWYLPIVPKFKRLFANGDDAKDLTWH 781
>BE440417
Length = 192
Score = 40.4 bits (93), Expect = 0.004
Identities = 20/44 (45%), Positives = 26/44 (58%)
Frame = +2
Query: 458 KVSWFDCHRQFLSEDHEYRKCPKKFTKGKGETSTKPRRLNGEEV 501
K+ +FD H QFL +H YR+ K F KG ETS P L+ +V
Sbjct: 35 KICYFDFHHQFLPLNHAYRRNKKSFKKGWVETSPPPPCLSSLDV 166
>TC229565 similar to PIR|T05694|T05694 pathogenesis-related protein
F20M13.220 - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (19%)
Length = 673
Score = 30.4 bits (67), Expect = 4.5
Identities = 22/80 (27%), Positives = 31/80 (38%), Gaps = 8/80 (10%)
Frame = +1
Query: 516 EGPSQGYGVKHH--------WTSRSIFWELPYWKTLLLPHNIDVMHTERNVFLNILFTVM 567
+GP +G+ H W S S+FW L +W L R ++ I
Sbjct: 304 QGPQHEHGLLLHQRSLFLWWWFSFSLFWSLSFWVPL---------S**RLNYILIFLLPF 456
Query: 568 DTKGKTKDTFKSRKDLEEYC 587
T KT+ R D +EYC
Sbjct: 457 HTDTKTRSIALIRSDKKEYC 516
>TC232917
Length = 610
Score = 30.4 bits (67), Expect = 4.5
Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 4/60 (6%)
Frame = +2
Query: 384 YLRPLIDELKMLWEDGVMTYDVSLKQNFLMKAAV----MWTINDFPAYGMLSGWMTMGKL 439
++ P ID+ + GV + D+ + QN L+ A + + TIN P G+LS W + ++
Sbjct: 113 FVPPSIDDP----QTGVSSKDIVISQNPLVSARIYLPKLTTINQVPILGLLSRWWLLFRI 280
>AW733327
Length = 419
Score = 30.4 bits (67), Expect = 4.5
Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 9/60 (15%)
Frame = -3
Query: 913 LLQKHKIKECVQQILVYVFEVGK------VISWTMITLGC*LTLL---SWSTLVVLQRSW 963
L+ H+I + + I++ + K +I W MI G ++ WSTL+VL R W
Sbjct: 288 LILGHEISDIIHYIIITIILYSKHIRPLLLIKWHMIAXGIHNMIIVKRCWSTLIVLYRWW 109
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.348 0.152 0.547
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,269,358
Number of Sequences: 63676
Number of extensions: 961444
Number of successful extensions: 8753
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 6353
Number of HSP's successfully gapped in prelim test: 280
Number of HSP's that attempted gapping in prelim test: 2245
Number of HSP's gapped (non-prelim): 6893
length of query: 1105
length of database: 12,639,632
effective HSP length: 107
effective length of query: 998
effective length of database: 5,826,300
effective search space: 5814647400
effective search space used: 5814647400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.8 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0112.9