BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0112.15
(994 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220322 107 3e-23
TC232149 57 1e-12
BF425842 71 2e-12
BM308209 50 1e-09
TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transp... 44 4e-04
TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F1... 42 0.001
BE806360 similar to GP|6175165|gb| Mutator-like transposase {Ara... 41 0.002
BM108247 weakly similar to PIR|H84710|H847 Mutator-like transpos... 36 0.095
TC217533 similar to UP|Q9LUS2 (Q9LUS2) Chloroplast outer envelop... 36 0.095
AI856054 35 0.16
BG882782 35 0.16
TC232838 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {A... 34 0.28
TC214054 similar to UP|Q75QC2 (Q75QC2) Glutamate-rich protein, p... 34 0.36
TC230134 weakly similar to UP|NUCL_HUMAN (P19338) Nucleolin (Pro... 33 0.81
TC232225 weakly similar to UP|ST11_YEAST (P23561) Serine/threoni... 32 1.1
TC230133 weakly similar to UP|Q6VBJ3 (Q6VBJ3) Epa4p, partial (6%) 32 1.4
BE822052 similar to GP|4557063|gb|A expressed protein {Arabidops... 32 1.8
TC207946 similar to UP|Q9LG09 (Q9LG09) F14J16.29, partial (24%) 31 2.3
TC223505 similar to UP|Q9FJR9 (Q9FJR9) Similarity to maturase-re... 31 2.3
CO980158 31 3.1
>TC220322
Length = 1264
Score = 107 bits (267), Expect = 3e-23
Identities = 55/168 (32%), Positives = 92/168 (54%)
Frame = +2
Query: 644 RAARASTLPEWYAAMEVMKELNEAAFQDMMNLPPKMWTRSAYSRDTQCDLQVNNMCEAFN 703
+ A+++T+ E+ M +K +N A++ + P + WT++ +S + D NN CE FN
Sbjct: 2 QCAKSTTVAEFEGHMAHLKTINCQAWEYLNKWPKQAWTKAHFSTIPKVDNICNNTCEVFN 181
Query: 704 RAILEYRDKPIITLLERLKFYLNNRIVKQKQLMLRWRTALCPMIQQKLEDSKRNSDKWFA 763
IL+YR KPIIT+LE ++ Y+ + +K + LC + ++LE +++W
Sbjct: 182 FRILQYRCKPIITMLEEIRSYIMRTMAARKVKLSGKPGPLCLVQYKRLEKEFHFANQWTP 361
Query: 764 NWMGDEYMSLFEVSRDNEKYSVNLESRSCACRRWDLSGIPCAHAVASM 811
W GD +EV K VNL +C C W L+G+PC HA+A++
Sbjct: 362 IWCGDNMGLRYEVHMWGNKVEVNLAGWTCTCGVWQLTGMPCRHAIATI 505
>TC232149
Length = 1005
Score = 57.0 bits (136), Expect(2) = 1e-12
Identities = 36/138 (26%), Positives = 60/138 (43%), Gaps = 30/138 (21%)
Frame = -3
Query: 714 IITLLERLKFYLNNRIVKQKQLMLRWRTALCPMIQQKLEDSKRNSDKWFANWMGDEYMSL 773
I+ + E ++ Y+ ++ K M L PM Q ++E K S+K ANW+GD +
Sbjct: 427 ILNMCEDIRTYIMCKMKSDKLKMATRLRPLVPMQQSRIEKGKVESNK*TANWVGDPDGTR 248
Query: 774 FEVSRDNEKYSVNLESRSCACRRWDLSGI------------------------------P 803
F+V + + +V+L++ S CR W L GI P
Sbjct: 247 FKVCHYDTRLNVDLQN*SFTCRMWQLIGIPLS*LNLSILDIMYCVTMNLMFFWFIYIGMP 68
Query: 804 CAHAVASMWFGRRVPEDY 821
C HA+ ++ + +PE Y
Sbjct: 67 CRHAIVAIRYNYHIPEGY 14
Score = 35.0 bits (79), Expect(2) = 1e-12
Identities = 23/97 (23%), Positives = 47/97 (47%)
Frame = -1
Query: 616 VEHRLCIKHLYGNFRKKYPGAEMKQALWRAARASTLPEWYAAMEVMKELNEAAFQDMMNL 675
++HR I + G+++ AR+ T E+ M+V+K +NE A+ +
Sbjct: 657 IKHRFSISEILGHYK--------------CARSPTELEFNVIMKVVKRMNEKAWAYLDKG 520
Query: 676 PPKMWTRSAYSRDTQCDLQVNNMCEAFNRAILEYRDK 712
P + WT++ +S + + E++N IL+ ++K
Sbjct: 519 PRESWTKAYFSENCK-------DYESYNAKILKLKNK 430
>BF425842
Length = 414
Score = 71.2 bits (173), Expect = 2e-12
Identities = 29/55 (52%), Positives = 40/55 (72%)
Frame = +3
Query: 498 CRPLIGLDGCFLKGMYGGQLLAAVGKDGNNQMFPIAFAVVEAETKASWSWFVDLL 552
C+PL+ +DG FL G Y LL A+G+DG+ FP+AFA+VE+ETK +W WF+ L
Sbjct: 75 CKPLVQVDGTFLTGKYNDTLLTAIGQDGSRDNFPLAFAIVESETKEAWIWFLHYL 239
>BM308209
Length = 435
Score = 50.1 bits (118), Expect(2) = 1e-09
Identities = 21/54 (38%), Positives = 36/54 (65%)
Frame = +2
Query: 659 EVMKELNEAAFQDMMNLPPKMWTRSAYSRDTQCDLQVNNMCEAFNRAILEYRDK 712
E +KE+N A+ ++ + K+W + +S T+CD+ +NN+C+AFN IL R+K
Sbjct: 116 EHIKEVNNEAYDWLIKVGTKLWRKHTFSYYTKCDVLMNNLCDAFNSTILCAREK 277
Score = 42.0 bits (97), Expect = 0.001
Identities = 23/56 (41%), Positives = 33/56 (58%), Gaps = 2/56 (3%)
Frame = +3
Query: 758 SDKWFANWMGDEYMSLFEVSRD--NEKYSVNLESRSCACRRWDLSGIPCAHAVASM 811
S WFA G +FEVS ++K+ VNL S SC+ W+++GI C HA A++
Sbjct: 276 SGNWFATLSGH---LVFEVSHSMLSDKFIVNLNSWSCS*NLWEITGILCRHACAAI 434
Score = 31.6 bits (70), Expect(2) = 1e-09
Identities = 14/41 (34%), Positives = 23/41 (55%), Gaps = 1/41 (2%)
Frame = +3
Query: 623 KHLYGNFRKKYPGAE-MKQALWRAARASTLPEWYAAMEVMK 662
+H+Y NF+ K+ G ++ + RAA+AS W M + K
Sbjct: 6 RHIYNNFKNKFVGGTLLRNLMMRAAKASFEEAWDEKMNISK 128
>TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transposase
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (12%)
Length = 617
Score = 43.9 bits (102), Expect = 4e-04
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = +2
Query: 785 VNLESRSCACRRWDLSGIPCAHAVASM 811
VN+ S SC+CR W L+GIPC+HA A++
Sbjct: 20 VNIGSHSCSCRDWQLNGIPCSHAAAAL 100
>TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;} , partial (6%)
Length = 814
Score = 42.4 bits (98), Expect = 0.001
Identities = 20/50 (40%), Positives = 29/50 (58%)
Frame = +1
Query: 772 SLFEVSRDNEKYSVNLESRSCACRRWDLSGIPCAHAVASMWFGRRVPEDY 821
S FEV R V++++ C+C+ W L+G+PC HA+A R P DY
Sbjct: 31 STFEV-RGESVDIVDIDNWDCSCKGWQLTGVPCCHAIAVFECVGRSPYDY 177
>BE806360 similar to GP|6175165|gb| Mutator-like transposase {Arabidopsis
thaliana}, partial (17%)
Length = 431
Score = 41.2 bits (95), Expect = 0.002
Identities = 31/120 (25%), Positives = 46/120 (37%), Gaps = 2/120 (1%)
Frame = +2
Query: 533 AFAVVEAETKASWSWFVDLLVNDLDMVERK--RWAFISDQQKVMLN*SCIH*SIIHLYY* 590
AF VV+ E +W WF+ L N L++ R +SD+QK
Sbjct: 2 AFGVVDEENDDNWMWFLSELHNLLEIHTENMLRLTILSDRQK------------------ 127
Query: 591 I*YLSYLYSCVMQGLVPTLAELVGDVEHRLCIKHLYGNFRKKYPGAEMKQALWRAARAST 650
G+V + H C++HL +FRK++ + LW AA A T
Sbjct: 128 -------------GIVDGVEASFPTAFHGFCMQHLSDSFRKEFNNTMLVNLLWEAANALT 268
>BM108247 weakly similar to PIR|H84710|H847 Mutator-like transposase
[imported] - Arabidopsis thaliana, partial (4%)
Length = 265
Score = 35.8 bits (81), Expect = 0.095
Identities = 22/80 (27%), Positives = 39/80 (48%)
Frame = +2
Query: 642 LWRAARASTLPEWYAAMEVMKELNEAAFQDMMNLPPKMWTRSAYSRDTQCDLQVNNMCEA 701
LW+A+ A+T + M ++E++ A + + P W + L N E
Sbjct: 26 LWKASHATTTIAFKEKMGEIEEVSPEAAKWLQQFXPSQWALVHFKGTRFGHLSSN--IEE 199
Query: 702 FNRAILEYRDKPIITLLERL 721
FN+ IL+ R+ PII ++ R+
Sbjct: 200 FNKWILDTRELPIIXVIXRI 259
>TC217533 similar to UP|Q9LUS2 (Q9LUS2) Chloroplast outer envelope
protein-like (Chloroplast outer envelope
membrane-associated protein Toc120), partial (35%)
Length = 1633
Score = 35.8 bits (81), Expect = 0.095
Identities = 25/76 (32%), Positives = 33/76 (42%)
Frame = +1
Query: 269 FEDEDGDSSDLDSPSDDEEEGRSKRLPRFKAVQDGEEVKFQVGHTFSSKDLFKQAVKDYA 328
F DED DLD S+ ++E LP FK + + K H K F +
Sbjct: 199 FGDEDSLDDDLDESSESDDENEHDDLPPFKPLTKAQVEKLSKAH---KKAYFDELEYREK 369
Query: 329 LQQKKDLTFKKDDKKR 344
L KK L K++KKR
Sbjct: 370 LLMKKQL---KEEKKR 408
>AI856054
Length = 173
Score = 35.0 bits (79), Expect = 0.16
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = -1
Query: 698 MCEAFNRAILEYRDKPIITLLERLKFYLNNR 728
+CE+FN ILE R+K I+++LE + YL R
Sbjct: 143 ICESFNSVILEAREKLIVSMLEDIHIYLMER 51
>BG882782
Length = 357
Score = 35.0 bits (79), Expect = 0.16
Identities = 18/64 (28%), Positives = 29/64 (45%), Gaps = 5/64 (7%)
Frame = -1
Query: 310 VGHTFSSKDLFKQAVKDYALQQKKDLTFKKDDKKRCVVICV-----AGCPFYLRLSKTDS 364
VG F SKD K A+K Y ++ + + + +V C + CPFY+R +
Sbjct: 213 VGMDFDSKDAVKNALKQYVMKVHQSFKVVETKSHKYIVCCPNNTEESPCPFYMRAILSKK 34
Query: 365 RSYW 368
+ W
Sbjct: 33 TNAW 22
>TC232838 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;} , partial (46%)
Length = 902
Score = 34.3 bits (77), Expect = 0.28
Identities = 30/111 (27%), Positives = 50/111 (45%), Gaps = 3/111 (2%)
Frame = +2
Query: 187 VNEPLNESEDESYIPGSDE-YEGESE--DVSVDDSDYEENWDWAEVLPAESLINIATADR 243
++E + E+E + + DE YE E D + DD D EE+ D E + + A
Sbjct: 239 LSEQVEENEVKVLVEEDDEEYESVDEVVDDAEDDEDEEEDDDDDEGDDNDDEEDDAPDGG 418
Query: 244 DPPFVQGSVDVGNGARIEEPATFSDFEDEDGDSSDLDSPSDDEEEGRSKRL 294
D + + G+ R EP + D+D D + + D+EE+G + L
Sbjct: 419 DDDDDEDDEEEGDVQRGGEPDDDDNDSDDDSDDDEDEDEEDEEEQGEEEDL 571
>TC214054 similar to UP|Q75QC2 (Q75QC2) Glutamate-rich protein, partial (68%)
Length = 1306
Score = 33.9 bits (76), Expect = 0.36
Identities = 26/108 (24%), Positives = 46/108 (42%), Gaps = 4/108 (3%)
Frame = +1
Query: 119 VNDAPNSAPVNDAPNSPEVVHEVP---NSAPVNDALNSSSALVHEVPNSAPVNDALNSSS 175
V+ + PV +AP + + EVP A A S++ E AP +++
Sbjct: 157 VSKTQETTPVTEAPATEQPAAEVPATEQPAAEAPAPESTTEAPKEETTEAPTETVEKTTT 336
Query: 176 ALVHEVPNSAPV-NEPLNESEDESYIPGSDEYEGESEDVSVDDSDYEE 222
+ E P PV E + E E P ++ E ++E+V + + +E
Sbjct: 337 EVAPEEPKEVPVETEEVVAKETEEEKPAEEKSEEKTEEVKEEAEEPKE 480
>TC230134 weakly similar to UP|NUCL_HUMAN (P19338) Nucleolin (Protein C23),
partial (8%)
Length = 581
Score = 32.7 bits (73), Expect = 0.81
Identities = 25/101 (24%), Positives = 41/101 (39%)
Frame = +2
Query: 188 NEPLNESEDESYIPGSDEYEGESEDVSVDDSDYEENWDWAEVLPAESLINIATADRDPPF 247
N+ +++ED+ D+ + ED DD D E+ + A +D DP
Sbjct: 260 NKDASDTEDDD----DDDDVNDGEDGDDDDEDDED-------FSGDDGGEEADSDDDP-- 400
Query: 248 VQGSVDVGNGARIEEPATFSDFEDEDGDSSDLDSPSDDEEE 288
+ G ++ D +D+D D D D DDEE+
Sbjct: 401 -----EANGGGESDDDDEDDDDDDDDNDEDDGDEDDDDEED 508
>TC232225 weakly similar to UP|ST11_YEAST (P23561) Serine/threonine-protein
kinase STE11 , partial (6%)
Length = 802
Score = 32.3 bits (72), Expect = 1.1
Identities = 44/173 (25%), Positives = 68/173 (38%), Gaps = 10/173 (5%)
Frame = +3
Query: 167 VNDALNSSSALVHEVPNSAPVNEPLNESEDESYIPGSDE---YEGESEDVSVDDSDYEEN 223
V+D+ S+ L+ ++ PLN SE+ES + E E E +V +S E
Sbjct: 75 VDDSTCSTWDLLRDLAPQGEEGLPLNRSENESEVAREVERGAVEKEEGEVGTRESPKREE 254
Query: 224 WDWAEVLPAESLINIATADRDPPFVQGSVDVGNGARIEEPATFSDFEDEDGDSSDLDSPS 283
+ N A + E +FS ++D SS D S
Sbjct: 255 EE------------------------------NVAGLSESCSFSTSNEDDSSSSTTDPTS 344
Query: 284 DD-EEEGRSKRLPRFKAVQDGEEVKFQVGHTFS------SKDLFKQAVKDYAL 329
++ +GR KR+ ++ Q GE F G +F S D F AVK+ +L
Sbjct: 345 NNISPQGRIKRIITAESWQKGE---FLGGGSFGSVYEGISDDGFFFAVKEVSL 494
>TC230133 weakly similar to UP|Q6VBJ3 (Q6VBJ3) Epa4p, partial (6%)
Length = 850
Score = 32.0 bits (71), Expect = 1.4
Identities = 24/85 (28%), Positives = 40/85 (46%)
Frame = +3
Query: 204 DEYEGESEDVSVDDSDYEENWDWAEVLPAESLINIATADRDPPFVQGSVDVGNGARIEEP 263
D+ + ++ + DD+D EE+ D++ E A +D DP G + +E
Sbjct: 288 DDDDDDNVNDGEDDNDDEEDEDFSGDDGGEE----ADSDDDPEANGGGGSDDDDGDDDED 455
Query: 264 ATFSDFEDEDGDSSDLDSPSDDEEE 288
D +++DGD D D DDE+E
Sbjct: 456 ---DDNDEDDGDEDDEDEGDDDEDE 521
>BE822052 similar to GP|4557063|gb|A expressed protein {Arabidopsis
thaliana}, partial (28%)
Length = 668
Score = 31.6 bits (70), Expect = 1.8
Identities = 29/104 (27%), Positives = 40/104 (37%)
Frame = -1
Query: 203 SDEYEGESEDVSVDDSDYEENWDWAEVLPAESLINIATADRDPPFVQGSVDVGNGARIEE 262
SDE E E +D +D + EE E + DP NGA
Sbjct: 518 SDEDEDEEDDHDTNDQNDEEG--------DEDFSGEGEEEADPE----DDPEANGAGGSN 375
Query: 263 PATFSDFEDEDGDSSDLDSPSDDEEEGRSKRLPRFKAVQDGEEV 306
SD +D+D + D D +DE+EG + +D EEV
Sbjct: 374 DDDDSDEDDDDDNDGDDDGEDEDEKEGEDE--------EDEEEV 267
>TC207946 similar to UP|Q9LG09 (Q9LG09) F14J16.29, partial (24%)
Length = 538
Score = 31.2 bits (69), Expect = 2.3
Identities = 21/49 (42%), Positives = 28/49 (56%), Gaps = 2/49 (4%)
Frame = -2
Query: 629 FRKKYPGAEMKQALWRAARASTLPEWYAAME--VMKELNEAAFQDMMNL 675
F K GA + WRAAR ++ E AM+ MK+L EA +D+MNL
Sbjct: 195 FGKGKFGAVLVCIRWRAARPKSMHEKEIAMKKAKMKQLLEAMAKDLMNL 49
>TC223505 similar to UP|Q9FJR9 (Q9FJR9) Similarity to maturase-related
protein, partial (10%)
Length = 809
Score = 31.2 bits (69), Expect = 2.3
Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 2/75 (2%)
Frame = +2
Query: 118 SVNDAPNSAPVNDAPNSPEVVHEVPNSAPVNDALNSSSALVHEVPNSAPVNDALNSSSAL 177
S+ +AP AP AP+ P P+S P + + SSA V ++ A ++ A
Sbjct: 206 SLTNAP--APTPPAPSLPVTPSTPPSSTPSSPSATPSSATVSNGTTKPTLSSAPPTTPAF 379
Query: 178 VHEVP--NSAPVNEP 190
P NSAP++ P
Sbjct: 380 PSPSPNANSAPLSNP 424
>CO980158
Length = 803
Score = 30.8 bits (68), Expect = 3.1
Identities = 29/120 (24%), Positives = 53/120 (44%), Gaps = 10/120 (8%)
Frame = -3
Query: 118 SVNDAPNSAPVNDAPNSPEVVHEVPNSAPVNDALNSSSALVHEVPNSAPVNDALNSSSAL 177
+++D+ S P+ + +S +D N+ + +++ P+ D + +S
Sbjct: 468 NIDDSSCSYPLVQVDEEEDYYDGTQHSDYESDDSNAENNPLNDYPDEVSEEDEEDENSEN 289
Query: 178 VHEVPNSAPVNEPLNE------SEDES---YIPGSDEYEG-ESEDVSVDDSDYEENWDWA 227
E + +E +E SED++ Y DEYEG S+DV D +ENW W+
Sbjct: 288 ESEREDGNTSSESSDENIEHGFSEDDADPLYDEDFDEYEGAHSDDVEHMD---DENWRWS 118
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.139 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,559,052
Number of Sequences: 63676
Number of extensions: 612773
Number of successful extensions: 4098
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 3845
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4032
length of query: 994
length of database: 12,639,632
effective HSP length: 107
effective length of query: 887
effective length of database: 5,826,300
effective search space: 5167928100
effective search space used: 5167928100
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 64 (29.3 bits)
Lotus: description of TM0112.15