BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0082b.8
(726 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228457 weakly similar to GB|AAQ65170.1|34365717|BT010547 At4g2... 575 e-164
BE824305 188 6e-48
TC224457 111 3e-42
BQ785194 68 2e-11
AW704900 similar to GP|10178249|d DNA repair protein RAD5 protei... 48 2e-05
TC221457 similar to UP|FIBG_XENLA (P17634) Fibrinogen gamma chai... 42 0.001
TC218937 similar to UP|PEXA_ARATH (Q9SYU4) Peroxisome assembly p... 42 0.001
TC216628 weakly similar to UP|Q8H0X2 (Q8H0X2) Expressed protein ... 42 0.001
CO982502 41 0.002
BE474559 41 0.002
BF595511 40 0.003
TC230666 similar to UP|O82767 (O82767) PRT1 protein, partial (11%) 40 0.003
TC226342 homologue to UP|NPM2_MOUSE (Q80W85) Nucleoplasmin 2, pa... 40 0.003
TC232120 weakly similar to GB|AAQ56803.1|34098841|BT010360 At2g4... 40 0.004
TC230475 40 0.005
TC216627 similar to UP|Q8H0X2 (Q8H0X2) Expressed protein (At1g18... 40 0.005
TC206224 similar to PIR|E86326|E86326 protein F18O14.3 [imported... 39 0.011
TC229016 similar to UP|HES1_MOUSE (P35428) Transcription factor ... 38 0.014
TC227743 similar to PIR|E86326|E86326 protein F18O14.3 [imported... 38 0.014
AW568183 38 0.014
>TC228457 weakly similar to GB|AAQ65170.1|34365717|BT010547 At4g21070
{Arabidopsis thaliana;} , partial (17%)
Length = 1430
Score = 575 bits (1482), Expect = e-164
Identities = 287/379 (75%), Positives = 317/379 (82%), Gaps = 1/379 (0%)
Frame = +2
Query: 348 GKSVIGDAAMQPNVIHVHRCCVDWAPQVYFVDETCKNLKAEVARGAKLKCSTCGLKGAAL 407
G+ V GDAAM+PNVI VHR C+DWAPQVYFV E KNLKAEVARGAKLKCS C LKGAAL
Sbjct: 20 GRXVTGDAAMKPNVIPVHRICIDWAPQVYFVGEVVKNLKAEVARGAKLKCSKCNLKGAAL 199
Query: 408 GCYVKSCKRTYHVPCAMDVSTCRWDQEKYLLLCPVHSNAKFPHEKSRPKKQATQEHPASA 467
GCYVKSC+RTYHVPCAMD+S CRWD E +LLLCPVHSN KFP EK R KK+ATQ+HP +
Sbjct: 200 GCYVKSCRRTYHVPCAMDISDCRWDHEDFLLLCPVHSNVKFPCEKIRSKKKATQKHPTFS 379
Query: 468 HLSSQLSNPLGAFHDDGKKVVFCGSALSDEEKGSIENLRVMAVVVSGRGEVLLINFASKV 527
HL S SN L A D KK+VFCGSALS+EEK V LIN+ASKV
Sbjct: 380 HLPSHHSNRLEASQDASKKLVFCGSALSNEEK------------------VFLINYASKV 505
Query: 528 GATVTKFWTSDVTHVIAATDANGACSRTLKVLMAILNGRWVLKIDWIKACMKVMSLVEEE 587
GATVTKFWTS+VTHVIAATDA+GACSRTLKVLMAILNGRWVLK+DWIKACM+ ++ VEEE
Sbjct: 506 GATVTKFWTSNVTHVIAATDAHGACSRTLKVLMAILNGRWVLKMDWIKACMQEINPVEEE 685
Query: 588 PYEISLDNQGCNGGPKAGRLSALANEPKLFSGLKFYFSGDYVSSYKEDLEDLVEVGGGAV 647
PYEI+LDNQGC GGPKAGRL ALANEPKLFSGLKFYFSGDYVS+YKEDLE+L+EVGGG V
Sbjct: 686 PYEINLDNQGCQGGPKAGRLRALANEPKLFSGLKFYFSGDYVSTYKEDLEELIEVGGGTV 865
Query: 648 LTSKDKLEAKRHEFE-VTSNLLIVYNLDPPQGSKLGDEVSTLWQRLNEAEDLAANTGSQV 706
L K++LEA RHE + +S LL+VYNLDPPQG KLG+EVS LWQRLN+AEDLAANT QV
Sbjct: 866 LRIKEELEAHRHECKGDSSKLLVVYNLDPPQGCKLGEEVSILWQRLNDAEDLAANT-LQV 1042
Query: 707 IGHTWILESIAACKLQPFI 725
IGHTWILESIAACKLQPF+
Sbjct: 1043IGHTWILESIAACKLQPFV 1099
>BE824305
Length = 771
Score = 188 bits (478), Expect = 6e-48
Identities = 98/200 (49%), Positives = 128/200 (64%), Gaps = 6/200 (3%)
Frame = -1
Query: 529 ATVTKFWTSDVTHVIAATDANGACSRTLKVLMAILNGRWVLKIDWIKACMKVMSLVEEEP 588
A V K W S VTHVIA+TD NGAC TLKVL+ IL G+W+L I+WIKACMK M ++EE
Sbjct: 723 AXVLKNWDSSVTHVIASTDENGACRXTLKVLLGILEGKWILNIEWIKACMKEMGPIDEEC 544
Query: 589 YEISLDNQGCNGGPKAGRLSALANEPKLFSGLKFYFSGDYVSSYKEDLEDLVEVGGGAVL 648
YEI++D G GP+ GRL L +PKLF G KFYF GD++ SYK L++LV GG +L
Sbjct: 543 YEINVDIHGIRDGPRLGRLRVLNKQPKLFYGYKFYFMGDFIPSYKGYLQNLVVAAGGIIL 364
Query: 649 TSK------DKLEAKRHEFEVTSNLLIVYNLDPPQGSKLGDEVSTLWQRLNEAEDLAANT 702
K + H ++ LI+Y+L+ P K + + QR ++AE LA++T
Sbjct: 363 HRKPVSGDQESTSPDMHTYQT----LIIYSLELPDKCKPSKKDTICSQRRHDAEVLASST 196
Query: 703 GSQVIGHTWILESIAACKLQ 722
GS V +TW+L SIAACKLQ
Sbjct: 195 GSNVASNTWVLNSIAACKLQ 136
>TC224457
Length = 424
Score = 111 bits (278), Expect(2) = 3e-42
Identities = 50/68 (73%), Positives = 59/68 (86%)
Frame = +1
Query: 1 MEDSGKPNNKSKLMNPWMLHFQKLALELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLS 60
M+DSG +K+KL+NPWMLHFQKLALELKCPLCLSLF++PVLLPC+HLFC+SCL D +
Sbjct: 25 MDDSG---SKTKLLNPWMLHFQKLALELKCPLCLSLFKRPVLLPCNHLFCNSCLADCITA 195
Query: 61 GSECAVCK 68
G CAVCK
Sbjct: 196GPGCAVCK 219
Score = 79.7 bits (195), Expect(2) = 3e-42
Identities = 38/69 (55%), Positives = 51/69 (73%)
Frame = +2
Query: 61 GSECAVCKTKYAQTDIRNVPFVENMVAIYRSLDASFCASMLQQRSNDDSRVLQQCQTFRD 120
G + + YAQTD+R+VPFVEN+VAIYRSLDA+FCAS+ Q S+ D RVL+ CQ +
Sbjct: 197 GLDVLFVRQTYAQTDVRHVPFVENVVAIYRSLDATFCASLFQPCSSGDGRVLEPCQAILN 376
Query: 121 SSYSNIKKA 129
S+ S++K A
Sbjct: 377 STSSSLKAA 403
>BQ785194
Length = 421
Score = 67.8 bits (164), Expect = 2e-11
Identities = 31/39 (79%), Positives = 35/39 (89%)
Frame = +2
Query: 574 IKACMKVMSLVEEEPYEISLDNQGCNGGPKAGRLSALAN 612
IKACM+ ++ VEEEPYEI+LDNQGC GGPKAGRL ALAN
Sbjct: 17 IKACMQEINPVEEEPYEINLDNQGCQGGPKAGRLRALAN 133
>AW704900 similar to GP|10178249|d DNA repair protein RAD5 protein
{Arabidopsis thaliana}, partial (13%)
Length = 428
Score = 47.8 bits (112), Expect = 2e-05
Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +1
Query: 29 KCPLCLSLFEKPVLLPCDHLFCDSCLVDS--SLSGSECAVCKTKYAQTDIRNVP 80
+CP+CL +FE VL PC H C CL+ S + + C VC+ ++ D+ P
Sbjct: 238 ECPICLEVFEDAVLTPCAHRLCRECLLSSWRNATSGLCPVCRKTISRLDLITAP 399
>TC221457 similar to UP|FIBG_XENLA (P17634) Fibrinogen gamma chain precursor,
partial (4%)
Length = 831
Score = 42.0 bits (97), Expect = 0.001
Identities = 39/171 (22%), Positives = 71/171 (40%)
Frame = +3
Query: 554 RTLKVLMAILNGRWVLKIDWIKACMKVMSLVEEEPYEISLDNQGCNGGPKAGRLSALANE 613
+T K L G +LK+DWI C+ ++++ E Y I L N+ + G N+
Sbjct: 54 QTTKFLYGCAVGASILKVDWITDCVASRTILQPEKYMI-LPNRKDMKWTRIGTTIHHRNQ 230
Query: 614 PKLFSGLKFYFSGDYVSSYKEDLEDLVEVGGGAVLTSKDKLEAKRHEFEVTSNLLIVYNL 673
+F + G S+ L +++ GGG V + LE E ++V
Sbjct: 231 KDIFERVGILLHGK--PSFCTKLACIIKHGGGHVFKTLQGLEWSTDEERTLVGAIVV--- 395
Query: 674 DPPQGSKLGDEVSTLWQRLNEAEDLAANTGSQVIGHTWILESIAACKLQPF 724
++ +T+ + L A ++ +WI++S+ + KL PF
Sbjct: 396 ---------EDKATISRHLKHC---AKERNIPIMPFSWIIKSLYSGKLLPF 512
>TC218937 similar to UP|PEXA_ARATH (Q9SYU4) Peroxisome assembly protein 10
(Peroxin-10) (AthPEX10) (Pex10p) (PER8), partial (73%)
Length = 1217
Score = 41.6 bits (96), Expect = 0.001
Identities = 16/48 (33%), Positives = 25/48 (51%)
Frame = +1
Query: 29 KCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDI 76
KC LCLS + P C H+FC +C+ + EC +C+T + +
Sbjct: 955 KCTLCLSNRQHPTATSCGHVFCWNCITEWCNEKPECPLCRTPITHSSL 1098
>TC216628 weakly similar to UP|Q8H0X2 (Q8H0X2) Expressed protein (At1g18660),
partial (50%)
Length = 872
Score = 41.6 bits (96), Expect = 0.001
Identities = 18/38 (47%), Positives = 20/38 (52%)
Frame = +2
Query: 27 ELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSEC 64
+ C LCL L +PV PC H FC SCL S G C
Sbjct: 575 DFDCTLCLKLLYEPVTTPCGHSFCRSCLFQSMDRGLFC 688
>CO982502
Length = 677
Score = 41.2 bits (95), Expect = 0.002
Identities = 16/48 (33%), Positives = 25/48 (51%)
Frame = -1
Query: 29 KCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDI 76
KC LCLS + P C H+FC +C+ + EC +C+T + +
Sbjct: 239 KCTLCLSNPQHPTATSCGHVFCWNCITEWCNEKPECPLCRTPITHSSL 96
>BE474559
Length = 414
Score = 41.2 bits (95), Expect = 0.002
Identities = 24/90 (26%), Positives = 47/90 (51%), Gaps = 5/90 (5%)
Frame = +3
Query: 3 DSGKPNNKSKLMNPWMLHFQKLAL--ELKCPLCLSLFEKP-VLLPCDHLFCDSCLVDS-S 58
DS + + P ++ + L + +++CP+CL + +K ++ C H FC C+ S
Sbjct: 3 DSDETPSSGSEEKPEFVYVELLEIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMR 182
Query: 59 LSGSECAVCKTKYA-QTDIRNVPFVENMVA 87
L +EC C+T A + +R+ P + ++A
Sbjct: 183LGNNECPACRTHCASRRSLRDDPNYDALIA 272
>BF595511
Length = 420
Score = 40.4 bits (93), Expect = 0.003
Identities = 18/55 (32%), Positives = 31/55 (55%), Gaps = 3/55 (5%)
Frame = +1
Query: 28 LKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGS---ECAVCKTKYAQTDIRNV 79
+KC +C ++ V+ C HLFC SC+ ++GS +C C T + D+++V
Sbjct: 88 IKCSICQDRAKEVVITKCYHLFCYSCI--QKVAGSRHRKCPQCSTSFGANDVKSV 246
>TC230666 similar to UP|O82767 (O82767) PRT1 protein, partial (11%)
Length = 1102
Score = 40.4 bits (93), Expect = 0.003
Identities = 18/68 (26%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +2
Query: 5 GKPNNKSKLMNPWMLHFQKLAL-ELKCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSE 63
G P K+ + +KL++ ++ C +C L PV+L C H++C +C+++
Sbjct: 359 GTPAKGKKMPQEELSVQRKLSVADVTCTMCKQLLFHPVVLNCGHVYCQTCVINIDDEMLR 538
Query: 64 CAVCKTKY 71
C VC++ +
Sbjct: 539 CKVCQSPH 562
>TC226342 homologue to UP|NPM2_MOUSE (Q80W85) Nucleoplasmin 2, partial (8%)
Length = 1326
Score = 40.4 bits (93), Expect = 0.003
Identities = 14/48 (29%), Positives = 24/48 (49%)
Frame = +3
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIR 77
C +C + P+LL C H+FC+ C+ + C +C+ D+R
Sbjct: 828 CAICQEKMQAPILLSCKHMFCEECVSEWFERERTCPLCRALVKPADLR 971
>TC232120 weakly similar to GB|AAQ56803.1|34098841|BT010360 At2g44950
{Arabidopsis thaliana;} , partial (7%)
Length = 715
Score = 40.0 bits (92), Expect = 0.004
Identities = 25/78 (32%), Positives = 38/78 (48%), Gaps = 10/78 (12%)
Frame = +2
Query: 12 KLMNPWMLHFQKLALEL-------KCPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGS-- 62
K N L +KL EL KC +C ++ V+ C HLFC SC+ ++GS
Sbjct: 32 KEQNEGFLVTEKLQQELEEYREIIKCSICQDRAKEVVITKCYHLFCYSCI--QKVAGSRH 205
Query: 63 -ECAVCKTKYAQTDIRNV 79
+C C T + D+++V
Sbjct: 206 RKCPQCGTSFGANDVKSV 259
>TC230475
Length = 528
Score = 39.7 bits (91), Expect = 0.005
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = +3
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIRN 78
C +C P+LL C H+FC+ C+ + C +C+ D+R+
Sbjct: 297 CAICQEKMHAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPADLRS 443
>TC216627 similar to UP|Q8H0X2 (Q8H0X2) Expressed protein (At1g18660),
partial (51%)
Length = 1037
Score = 39.7 bits (91), Expect = 0.005
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +1
Query: 39 KPVLLPCDHLFCDSCLVDSSLSGSECAVCKT 69
+PV PC H FC SCL S G++C +C+T
Sbjct: 7 RPVTTPCGHSFCCSCLFQSMDRGNKCPLCRT 99
>TC206224 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(43%)
Length = 1080
Score = 38.5 bits (88), Expect = 0.011
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 3/45 (6%)
Frame = +1
Query: 27 ELKCPLCLSLFEKPVLLPCDHLFCDSCL---VDSSLSGSECAVCK 68
+ +C +C L + PV+ C HLFC CL + EC VCK
Sbjct: 307 DFECNICFDLAQDPVITLCGHLFCWPCLYRWLHHHSHSQECPVCK 441
>TC229016 similar to UP|HES1_MOUSE (P35428) Transcription factor HES-1 (Hairy
and enhancer of split 1), partial (6%)
Length = 769
Score = 38.1 bits (87), Expect = 0.014
Identities = 13/50 (26%), Positives = 27/50 (54%)
Frame = +1
Query: 30 CPLCLSLFEKPVLLPCDHLFCDSCLVDSSLSGSECAVCKTKYAQTDIRNV 79
CP+C+S + + C H+FC +C+ + + ++C C+ K + + V
Sbjct: 415 CPICMSPLVEEMSTRCGHIFCKNCIRAAISAQAKCPTCRKKVTKNSLIRV 564
>TC227743 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(73%)
Length = 1212
Score = 38.1 bits (87), Expect = 0.014
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 3/47 (6%)
Frame = +2
Query: 25 ALELKCPLCLSLFEKPVLLPCDHLFCDSCL---VDSSLSGSECAVCK 68
A +C +C L + P++ C HLFC CL + EC VCK
Sbjct: 203 AANFECNICFELAQDPIITLCGHLFCWPCLYKWLHFHSQSRECPVCK 343
>AW568183
Length = 429
Score = 38.1 bits (87), Expect = 0.014
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = +1
Query: 327 CSFCQSSETSEATGPMLHYANGKSVIGD 354
C FC SSE S A+GPM+HY +G+ V D
Sbjct: 346 CVFCLSSEES*ASGPMVHYLDGRPVTSD 429
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.132 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,630,395
Number of Sequences: 63676
Number of extensions: 541283
Number of successful extensions: 2903
Number of sequences better than 10.0: 116
Number of HSP's better than 10.0 without gapping: 2866
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2896
length of query: 726
length of database: 12,639,632
effective HSP length: 104
effective length of query: 622
effective length of database: 6,017,328
effective search space: 3742778016
effective search space used: 3742778016
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0082b.8