BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0082b.2
(153 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM567913 202 7e-53
TC231444 167 2e-42
BE473761 weakly similar to GP|27450574|gb| pollen-specific calmo... 151 1e-37
TC212512 126 5e-30
BQ454144 weakly similar to GP|9802536|gb| F17L21.25 {Arabidopsis... 101 1e-22
TC210367 similar to UP|Q8GZN1 (Q8GZN1) Pollen-specific calmoduli... 91 2e-19
AW308911 similar to GP|19913115|em SPY protein {Lycopersicon esc... 45 1e-05
TC205633 similar to UP|O81280 (O81280) Peroxisomal targeting sig... 33 0.053
BQ299342 similar to GP|18139887|gb O-linked N-acetyl glucosamine... 33 0.070
TC207489 weakly similar to UP|Q94AA8 (Q94AA8) AT5g63200/MDC12_17... 30 0.45
TC233771 30 0.59
TC205632 similar to UP|O82444 (O82444) Peroxisomal targeting seq... 29 1.0
TC230777 similar to GB|BAB08884.1|9758397|AB022212 signal recogn... 28 1.3
TC205470 28 1.3
TC230936 weakly similar to UP|Q9AWT3 (Q9AWT3) Nucleoid DNA-bindi... 28 1.3
TC210267 similar to UP|Q8LGU6 (Q8LGU6) HOBBIT protein, partial (... 27 2.9
TC231862 similar to GB|AAL60196.1|18139887|AF441079 O-linked N-a... 27 2.9
TC215378 similar to UP|Q94EJ1 (Q94EJ1) At1g22700/T22J18_13, part... 27 2.9
TC218992 similar to UP|OM20_SOLTU (P92792) Mitochondrial import ... 27 3.8
TC220549 similar to UP|EOL1_ARATH (Q9ZQX6) ETO1-like protein 1 (... 27 3.8
>BM567913
Length = 430
Score = 202 bits (513), Expect = 7e-53
Identities = 95/120 (79%), Positives = 107/120 (89%)
Frame = +2
Query: 1 DYRDYARNLEEEIWHDLAYAYISLSQWHDAEVCLSKSKAFRQYTASRCHVIGTMHEAKGL 60
DY D+AR++E EIWHDLA+ YISLS+WHDAEVCLSKSKA + Y+ASRCH IGTMHEAKGL
Sbjct: 71 DYIDHARSMEVEIWHDLAFVYISLSRWHDAEVCLSKSKAIKLYSASRCHAIGTMHEAKGL 250
Query: 61 YKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRYDRLNASAWYN 120
YKEA+KAFRDALNIDP HVPSLIS AV LR SN+S+P +RSFLMDALR+DR NASAWYN
Sbjct: 251 YKEALKAFRDALNIDPGHVPSLISAAVVLRWCSNRSNPAIRSFLMDALRHDRFNASAWYN 430
>TC231444
Length = 966
Score = 167 bits (422), Expect = 2e-42
Identities = 79/102 (77%), Positives = 91/102 (88%)
Frame = +1
Query: 52 GTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRYD 111
GTM+EAKGLYKEA+KAFRDAL+IDP HVPSLISTAV LRR SNQS+P +RSFLMDALR+D
Sbjct: 463 GTMYEAKGLYKEAIKAFRDALSIDPGHVPSLISTAVVLRRCSNQSNPAIRSFLMDALRHD 642
Query: 112 RLNASAWYNLGILHKAEGRVLEAVECFQAANSLEETAPIEPF 153
R NASAWYNLGI +K EG +LEA +CF+ AN LEE+AP+EPF
Sbjct: 643 RFNASAWYNLGIFNKDEGTILEAADCFETANFLEESAPVEPF 768
>BE473761 weakly similar to GP|27450574|gb| pollen-specific
calmodulin-binding protein {Arabidopsis thaliana},
partial (6%)
Length = 463
Score = 151 bits (381), Expect = 1e-37
Identities = 72/96 (75%), Positives = 82/96 (85%)
Frame = +3
Query: 3 RDYARNLEEEIWHDLAYAYISLSQWHDAEVCLSKSKAFRQYTASRCHVIGTMHEAKGLYK 62
RD ARNLE EIWHD+AY YISL QWHDAEVCLSKSKA + +ASRCH IG M+EAKG YK
Sbjct: 174 RDRARNLEVEIWHDIAYVYISLLQWHDAEVCLSKSKAIKPLSASRCHAIGIMYEAKGHYK 353
Query: 63 EAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDP 98
EA+KAF DAL++DP HVPSLISTAV L+R SN+S+P
Sbjct: 354 EALKAFGDALDVDPGHVPSLISTAVVLKRCSNKSNP 461
>TC212512
Length = 1090
Score = 126 bits (316), Expect = 5e-30
Identities = 63/104 (60%), Positives = 80/104 (76%), Gaps = 2/104 (1%)
Frame = +2
Query: 52 GTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRYD 111
G + EA+G ++EA+K+FR AL+I+P HVPSLISTA LR+ QS +VRS L DALR D
Sbjct: 623 GLLSEARGFHQEALKSFRKALDIEPNHVPSLISTACVLRQLGGQSSSIVRSLLTDALRLD 802
Query: 112 RLNASAWYNLGILHKAE--GRVLEAVECFQAANSLEETAPIEPF 153
R N SAWYNLG+L+KA+ +EAVECF+AA LEE++PIEPF
Sbjct: 803 RTNPSAWYNLGLLYKADLGTSAMEAVECFEAAALLEESSPIEPF 934
>BQ454144 weakly similar to GP|9802536|gb| F17L21.25 {Arabidopsis thaliana},
partial (15%)
Length = 424
Score = 101 bits (252), Expect = 1e-22
Identities = 49/118 (41%), Positives = 77/118 (64%)
Frame = +1
Query: 36 KSKAFRQYTASRCHVIGTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQ 95
KS++ ++ H+ G + EA+ L+KEA +F +L+I+P ++P +ISTA +
Sbjct: 1 KSQSIEFFSPRSWHITGLLFEAQSLHKEAFVSFSVSLSIEPDYIPCIISTAKLFLKLGIP 180
Query: 96 SDPVVRSFLMDALRYDRLNASAWYNLGILHKAEGRVLEAVECFQAANSLEETAPIEPF 153
S P+ RSFLM+ALR D N AW+NLG++ K EG + +A +CFQAA L+ +AP++ F
Sbjct: 181 SLPIARSFLMNALRLDPTNHDAWFNLGLVSKMEGSLQQAADCFQAAYELKLSAPVQKF 354
>TC210367 similar to UP|Q8GZN1 (Q8GZN1) Pollen-specific calmodulin-binding
protein, partial (8%)
Length = 610
Score = 91.3 bits (225), Expect = 2e-19
Identities = 44/102 (43%), Positives = 65/102 (63%)
Frame = +3
Query: 52 GTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRYD 111
G + E +G +EA+ A +A+ ++P +VP I +++ + + RS L DALR +
Sbjct: 18 GVLFEGRGQNEEALCATINAILLEPNYVPCKILMGALIQKLGTKHLAIARSLLSDALRIE 197
Query: 112 RLNASAWYNLGILHKAEGRVLEAVECFQAANSLEETAPIEPF 153
N AWYNLG+LHK EGR+ +A +CFQAA+ LEE+ PIE F
Sbjct: 198 PTNRKAWYNLGLLHKHEGRISDAADCFQAASMLEESDPIESF 323
>AW308911 similar to GP|19913115|em SPY protein {Lycopersicon esculentum},
partial (15%)
Length = 444
Score = 45.1 bits (105), Expect = 1e-05
Identities = 28/85 (32%), Positives = 46/85 (53%)
Frame = +1
Query: 57 AKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRYDRLNAS 116
++ + +A+ + L D +V +LI + L+ N+ SF +A++ D NA
Sbjct: 7 SRNKFVDALAIYERVLESDGANVEALIGKGICLQM-QNKGRLAYESF-SEAIKVDPQNAC 180
Query: 117 AWYNLGILHKAEGRVLEAVECFQAA 141
A + GILHK EGR++EA E +Q A
Sbjct: 181 ALTHCGILHKDEGRLVEAAESYQKA 255
Score = 38.5 bits (88), Expect = 0.001
Identities = 23/81 (28%), Positives = 43/81 (52%), Gaps = 7/81 (8%)
Frame = +1
Query: 52 GTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTAVAL-------RRWSNQSDPVVRSFL 104
G +H+ +G EA ++++ AL +DP + + A+ L + N D + + F
Sbjct: 196 GILHKDEGRLVEAAESYQKALQVDPSYKAAAECLAIVLTDIGTNIKLAGNTQDGIQKYF- 372
Query: 105 MDALRYDRLNASAWYNLGILH 125
+AL+ D A A+YNLG+++
Sbjct: 373 -EALKVDPHYAPAYYNLGVVY 432
>TC205633 similar to UP|O81280 (O81280) Peroxisomal targeting signal-1
receptor, partial (64%)
Length = 1643
Score = 33.1 bits (74), Expect = 0.053
Identities = 26/99 (26%), Positives = 46/99 (46%)
Frame = +1
Query: 50 VIGTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALR 109
V+G M+ Y +A+ +F AL + P+ SL + A + S QS + ++ AL
Sbjct: 937 VLGVMYNLSREYDKAIASFERALKLKPQDY-SLWNKLGATQANSVQSADAIMAY-QQALD 1110
Query: 110 YDRLNASAWYNLGILHKAEGRVLEAVECFQAANSLEETA 148
AW N+GI + +G E++ + A ++ A
Sbjct: 1111 LKPNYVRAWANMGISYANQGMYDESIRYYVRALAMNPKA 1227
>BQ299342 similar to GP|18139887|gb O-linked N-acetyl glucosamine transferase
{Arabidopsis thaliana}, partial (14%)
Length = 421
Score = 32.7 bits (73), Expect = 0.070
Identities = 29/138 (21%), Positives = 49/138 (35%)
Frame = +3
Query: 12 EIWHDLAYAYISLSQWHDAEVCLSKSKAFRQYTASRCHVIGTMHEAKGLYKEAVKAFRDA 71
+ W +LA AY+ + +A C ++ A +G + +A+GL +EA + +A
Sbjct: 48 DAWSNLASAYMRKGRLTEAAQCCRQALAINPLMVDAHSNLGNLMKAQGLVQEAYSCYLEA 227
Query: 72 LNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRYDRLNASAWYNLGILHKAEGRV 131
L I P A AW NL L G
Sbjct: 228 LRIQPTF------------------------------------AIAWSNLAGLFMESGDF 299
Query: 132 LEAVECFQAANSLEETAP 149
A++ ++ A L+ + P
Sbjct: 300 NRALQYYKEAVKLKPSFP 353
>TC207489 weakly similar to UP|Q94AA8 (Q94AA8) AT5g63200/MDC12_17, partial
(40%)
Length = 800
Score = 30.0 bits (66), Expect = 0.45
Identities = 25/128 (19%), Positives = 52/128 (40%)
Frame = +1
Query: 14 WHDLAYAYISLSQWHDAEVCLSKSKAFRQYTASRCHVIGTMHEAKGLYKEAVKAFRDALN 73
WH L + Q+ ++ L + A + + +G + +A + ++ AL+
Sbjct: 151 WHQLGIHSLCARQFKTSQKYLKAAVACDKDCSYAWATLGVSLQLSEEPSQAEEVYKQALS 330
Query: 74 IDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRYDRLNASAWYNLGILHKAEGRVLE 133
+ +I + + + + ++ +L A A+ NLG++ AEG + E
Sbjct: 331 LATTKQAHVILSNLGILYRHQKQYQRAKAMFTKSLELQPGYAPAFNNLGLVFVAEGLLEE 510
Query: 134 AVECFQAA 141
A CF A
Sbjct: 511 AKYCFDKA 534
>TC233771
Length = 390
Score = 29.6 bits (65), Expect = 0.59
Identities = 11/43 (25%), Positives = 24/43 (55%)
Frame = +2
Query: 56 EAKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDP 98
E +G+ + + F+ A N+DP + +L++ ++ W N+ P
Sbjct: 17 ELQGIAMKRISMFKFAGNLDPTYQKTLVNAMISFFEWYNKLHP 145
>TC205632 similar to UP|O82444 (O82444) Peroxisomal targeting sequence 1
receptor (Fragment), partial (24%)
Length = 707
Score = 28.9 bits (63), Expect = 1.0
Identities = 26/97 (26%), Positives = 42/97 (42%)
Frame = +2
Query: 52 GTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRYD 111
G M+ Y A +F AL + P+ SL + A + S QS + ++ AL
Sbjct: 2 GVMYXXSREYDXAXXSFERALKLKPQDY-SLWNKLGATQANSVQSADAIMAY-QQALDLK 175
Query: 112 RLNASAWYNLGILHKAEGRVLEAVECFQAANSLEETA 148
AW N+GI + +G E+V + A ++ A
Sbjct: 176 PNYVRAWANMGISYANQGMYDESVRYYVRALAMNPKA 286
>TC230777 similar to GB|BAB08884.1|9758397|AB022212 signal recognition
particle 68kD protein-like {Arabidopsis thaliana;} ,
partial (47%)
Length = 1077
Score = 28.5 bits (62), Expect = 1.3
Identities = 21/47 (44%), Positives = 21/47 (44%), Gaps = 4/47 (8%)
Frame = +1
Query: 30 AEVCLSKSKAFRQ----YTASRCHVIGTMHEAKGLYKEAVKAFRDAL 72
AEVC KS AFR Y A V G EA LY A DAL
Sbjct: 409 AEVCSCKSLAFRAQRCFYVAKSYSVAGKRAEAYALYCRARNLADDAL 549
>TC205470
Length = 580
Score = 28.5 bits (62), Expect = 1.3
Identities = 18/79 (22%), Positives = 36/79 (44%), Gaps = 4/79 (5%)
Frame = +2
Query: 51 IGTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTAVALRRWSNQSDPVVRSFLMDALRY 110
I + + G K +K DA+ + R V + S + ++ W++ ++ S L +
Sbjct: 20 INWLRKMSGKRKRKLKLLSDAITDNRRTVAEVKSENLEMQSWADLPAELLESILSRLILA 199
Query: 111 DRLNASA----WYNLGILH 125
D + ASA W+++ H
Sbjct: 200 DNIRASAVCRRWHSVASDH 256
>TC230936 weakly similar to UP|Q9AWT3 (Q9AWT3) Nucleoid DNA-binding protein
cnd41-like protein, partial (54%)
Length = 985
Score = 28.5 bits (62), Expect = 1.3
Identities = 23/72 (31%), Positives = 33/72 (44%), Gaps = 2/72 (2%)
Frame = -2
Query: 67 AFRDALNIDPRHVPSLISTAVALRRWSNQSD-PVVRSFLMDALRYDRLNASAWYNLGI-L 124
AF +DP HV S + V L RW + SD V + + R +R LG+ L
Sbjct: 339 AFGARRALDPAHVASRVENHVGLTRWLSDSDSEEVLAASLTRSRDNRALQRVGLELGL*L 160
Query: 125 HKAEGRVLEAVE 136
+ E RV +A +
Sbjct: 159 GRVEVRVRDAFQ 124
>TC210267 similar to UP|Q8LGU6 (Q8LGU6) HOBBIT protein, partial (39%)
Length = 1273
Score = 27.3 bits (59), Expect = 2.9
Identities = 20/78 (25%), Positives = 40/78 (50%), Gaps = 3/78 (3%)
Frame = +3
Query: 51 IGTMHEAKGLYKEAVKAFRDALNIDPRHVPSLISTA---VALRRWSNQSDPVVRSFLMDA 107
+G + + ++ A+K F+ A+ ++PR + VAL + N ++ + A
Sbjct: 219 MGNCYSLQKDHETALKNFQRAVQLNPRFAYAHTLCGHEYVALEDFENG----IKCY-QSA 383
Query: 108 LRYDRLNASAWYNLGILH 125
LR D + +AWY LG+++
Sbjct: 384 LRVDARHYNAWYGLGMVY 437
>TC231862 similar to GB|AAL60196.1|18139887|AF441079 O-linked N-acetyl
glucosamine transferase {Arabidopsis thaliana;} ,
partial (25%)
Length = 750
Score = 27.3 bits (59), Expect = 2.9
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 117 AWYNLGILHKAEGRVLEAVECFQAANSLEETAP 149
A+ NLG K GRV EA++C+ +L+ P
Sbjct: 87 AYNNLGNALKDVGRVEEAIQCYNQCLTLQPNHP 185
>TC215378 similar to UP|Q94EJ1 (Q94EJ1) At1g22700/T22J18_13, partial (55%)
Length = 807
Score = 27.3 bits (59), Expect = 2.9
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = +3
Query: 51 IGTMHEAKGLYKEAVKAFRDALNIDPRH 78
+G E+K YK A+KAF + L DP +
Sbjct: 357 LGDAFESKKDYKSALKAFEEVLLFDPNN 440
>TC218992 similar to UP|OM20_SOLTU (P92792) Mitochondrial import receptor
subunit TOM20 (Translocase of outer membrane 20 kDa
subunit), partial (59%)
Length = 561
Score = 26.9 bits (58), Expect = 3.8
Identities = 15/51 (29%), Positives = 25/51 (48%)
Frame = +3
Query: 28 HDAEVCLSKSKAFRQYTASRCHVIGTMHEAKGLYKEAVKAFRDALNIDPRH 78
HD CL + F + +I + EAKG + +A++ F+ A DP +
Sbjct: 45 HDTLWCLGNANTFYAF------LIPDITEAKGYFDKALEYFQKAAEEDPEN 179
>TC220549 similar to UP|EOL1_ARATH (Q9ZQX6) ETO1-like protein 1 (Ethylene
overproducer 1-like protein 1), partial (26%)
Length = 876
Score = 26.9 bits (58), Expect = 3.8
Identities = 16/45 (35%), Positives = 24/45 (52%)
Frame = +3
Query: 34 LSKSKAFRQYTASRCHVIGTMHEAKGLYKEAVKAFRDALNIDPRH 78
LS++ AF+ H+ HE KG A++ R AL++DP H
Sbjct: 510 LSRAIAFKA-DLHLLHLRAAFHEHKGDVLGAIRDCRAALSVDPFH 641
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.133 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,709,797
Number of Sequences: 63676
Number of extensions: 77627
Number of successful extensions: 397
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 392
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 396
length of query: 153
length of database: 12,639,632
effective HSP length: 89
effective length of query: 64
effective length of database: 6,972,468
effective search space: 446237952
effective search space used: 446237952
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0082b.2