BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0076a.12
(430 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228022 weakly similar to UP|Q93Z38 (Q93Z38) At1g34060/F12G12_1... 256 1e-68
BG362780 196 1e-50
BU547047 158 4e-39
CO981522 157 7e-39
BG156691 128 6e-30
AW759622 63 3e-10
TC226060 similar to GB|AAP68293.1|31711874|BT008854 At1g80360 {A... 39 0.005
BU763775 34 0.15
TC219250 similar to UP|O82030 (O82030) Histidinol-phosphate amin... 30 1.6
TC230352 30 2.1
TC231666 weakly similar to UP|A4E1_HUMAN (Q9UPM8) Adapter-relate... 30 2.7
TC225638 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein... 29 4.7
TC225636 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein... 29 4.7
TC212806 weakly similar to UP|A4E1_HUMAN (Q9UPM8) Adapter-relate... 28 8.0
AW308849 weakly similar to SP|O49046|ARGI_ Arginase (EC 3.5.3.1)... 28 8.0
TC230772 similar to UP|O24648 (O24648) Gibberellin 3 beta-hydrox... 28 8.0
NP005385 DNA-directed RNA polymerase 28 8.0
>TC228022 weakly similar to UP|Q93Z38 (Q93Z38) At1g34060/F12G12_150, partial
(54%)
Length = 1272
Score = 256 bits (654), Expect = 1e-68
Identities = 144/367 (39%), Positives = 212/367 (57%), Gaps = 9/367 (2%)
Frame = +1
Query: 68 NLDKGDPLVFGKYWKKKSEECMVVIKGCELMSY-LSDTSNVCWYMLPELKDAIERLHHVV 126
N GDP +W + + +++ G M Y SD S Y+ L + I++LH +
Sbjct: 43 NAGSGDPYFMEPFWMRHAAGSAILVSGWHRMGYSYSDGS----YISQLLVEYIKKLHGIG 210
Query: 127 RNAVTKDKYIVIGTGTTQLFMAALFALSPSMTTSHPINVVSATPYYSEYKDQLDLFCSER 186
NA+T+ KYIV G+G+TQL AA++ALSP+ + S P VV+ PYY Y+ Q F S
Sbjct: 211 GNAITEGKYIVFGSGSTQLLNAAVYALSPNSSMS-PAKVVATAPYYPVYRTQTQFFNSRD 387
Query: 187 FQWGGDAGEY----DKNETYIEVVNYPNNPDGTIKEPMVKSIVEG---KLVHDLAYYWPH 239
F + G+ + DKN +IE V PNNPDG + K ++EG K ++D AYYWPH
Sbjct: 388 FSYEGETSSWKNKTDKNSIFIEFVTSPNNPDGKL----TKEVLEGPNVKSIYDRAYYWPH 555
Query: 240 YTPITHQANHDVMLFTLSKCTGHAGSRIGWAIVKDIEVAKKMVMFVQMSSIGVAKESQTR 299
+T I A+ D+M+FT+SK TGHAGSR GWAIVKD V +KM+ ++ M+++GV++E+Q R
Sbjct: 556 FTAIPSPADDDLMIFTISKLTGHAGSRFGWAIVKDEAVYEKMLTYMDMNTMGVSREAQLR 735
Query: 300 AAKIMGVICDGYQKFESIEPDQLFFEYCKNILRERWEKLRRSIELRKVFTVAKYPKAYCN 359
A K++ V +G K F++ + +R+RW +L+ I K F++ K YC
Sbjct: 736 ALKLLDVALEGDGK--------EIFQFAYSTMRDRWIRLKEIISKTKRFSLQKISSQYCT 891
Query: 360 FANESFEALPAFVWLKCE-EGIENAENYLGKLKIRGRGGVRFGAGSKHVRLSMIGTDDEF 418
F +A PA+ WLKCE + N L I GR G + A +++VRLS+I + D+F
Sbjct: 892 FFKRDRDASPAYAWLKCERQQDNNCYEILEAAGINGREGSLYSADNRYVRLSLIRSQDDF 1071
Query: 419 TELIKRL 425
LI +L
Sbjct: 1072EILINKL 1092
>BG362780
Length = 498
Score = 196 bits (499), Expect = 1e-50
Identities = 97/163 (59%), Positives = 122/163 (74%)
Frame = +1
Query: 119 IERLHHVVRNAVTKDKYIVIGTGTTQLFMAALFALSPSMTTSHPINVVSATPYYSEYKDQ 178
+ RLH+VV NAVT+ ++IV+GTG++QLF+AAL+ALSP+ + PI+VV ATPYYS Y
Sbjct: 13 VVRLHNVVGNAVTEGRHIVVGTGSSQLFLAALYALSPT-DSPEPISVVCATPYYSSYPSM 189
Query: 179 LDLFCSERFQWGGDAGEYDKNETYIEVVNYPNNPDGTIKEPMVKSIVEGKLVHDLAYYWP 238
D S ++WGGDA Y+K YIE+V PNNPDG ++ V +G LVHDLAYYWP
Sbjct: 190 TDHLKSGLYKWGGDAESYEKEGPYIELVTSPNNPDGHVRRSKVNRS-QGFLVHDLAYYWP 366
Query: 239 HYTPITHQANHDVMLFTLSKCTGHAGSRIGWAIVKDIEVAKKM 281
YTPI+ A+HD+ LFT+SK TGHAG RIGWA+VKD EVAKKM
Sbjct: 367 QYTPISAPADHDLTLFTVSKSTGHAGMRIGWALVKDQEVAKKM 495
>BU547047
Length = 559
Score = 158 bits (400), Expect = 4e-39
Identities = 78/164 (47%), Positives = 113/164 (68%)
Frame = -2
Query: 263 AGSRIGWAIVKDIEVAKKMVMFVQMSSIGVAKESQTRAAKIMGVICDGYQKFESIEPDQL 322
AG RIGWAIVKD EVAKKM F+++S+IGV+K+SQ RAAK++ + D ++ E+ + +
Sbjct: 558 AGMRIGWAIVKDKEVAKKMTKFIEISTIGVSKDSQLRAAKVLKAVSDSCEQ-ENSQDGES 382
Query: 323 FFEYCKNILRERWEKLRRSIELRKVFTVAKYPKAYCNFANESFEALPAFVWLKCEEGIEN 382
FF + NI+ +RW++LR +E +FT+ ++ A+C F + E PAF+WLKCE IE+
Sbjct: 381 FFTHSYNIMAQRWKQLRAVVEAGDLFTLPQFSPAFCTFFGQETEPQPAFIWLKCEGDIED 202
Query: 383 AENYLGKLKIRGRGGVRFGAGSKHVRLSMIGTDDEFTELIKRLS 426
E+ L + KI R G FGA K+VR+SM+ TD+ F +LI RLS
Sbjct: 201 CESLLREHKIVARSGRHFGASPKYVRISMLDTDETFIQLIDRLS 70
>CO981522
Length = 927
Score = 157 bits (398), Expect = 7e-39
Identities = 84/205 (40%), Positives = 121/205 (58%), Gaps = 4/205 (1%)
Frame = -2
Query: 68 NLDKGDPLVFGKYWKKKSEECMVVIKGCELMSYLSDTSNVCWYMLPELKDAIERLHHVVR 127
N D GDP +W + + +++ G MSY +V +L E I+++H +V
Sbjct: 692 NADGGDPYFMEPFWMQHAASSAILVSGWHRMSYSYSDGSVISQLLVEY---IKKVHGIVG 522
Query: 128 NAVTKDKYIVIGTGTTQLFMAALFALSPSMTTSHPINVVSATPYYSEYKDQLDLFCSERF 187
NA+T+ KYIV G+G+TQL AA++ALSP + S P VV+ PYY Y++Q F S F
Sbjct: 521 NAITEGKYIVFGSGSTQLLNAAVYALSPDPSMS-PSKVVATAPYYPLYREQTQFFNSRDF 345
Query: 188 QWGGDAGEYDKNET----YIEVVNYPNNPDGTIKEPMVKSIVEGKLVHDLAYYWPHYTPI 243
+ GD + N +IE V PNNPDG + + ++K + K ++D AYYWPH+T I
Sbjct: 344 SYEGDTSLWKNNTNSSFRFIEFVTSPNNPDGKLNKGILKGS-DVKTIYDRAYYWPHFTAI 168
Query: 244 THQANHDVMLFTLSKCTGHAGSRIG 268
A+ D+MLF++SK TGHAGSR G
Sbjct: 167 PSPADDDLMLFSISKLTGHAGSRFG 93
>BG156691
Length = 457
Score = 128 bits (321), Expect = 6e-30
Identities = 66/152 (43%), Positives = 98/152 (64%)
Frame = +2
Query: 202 YIEVVNYPNNPDGTIKEPMVKSIVEGKLVHDLAYYWPHYTPITHQANHDVMLFTLSKCTG 261
YIE+VN+ P T K +G L+HDLAYYWP YTPI+ ++HD+ LFT+SK T
Sbjct: 5 YIELVNFYQ*P*WT*KGSYGNRS-QGLLIHDLAYYWPQYTPISSPSDHDLTLFTVSKSTA 181
Query: 262 HAGSRIGWAIVKDIEVAKKMVMFVQMSSIGVAKESQTRAAKIMGVICDGYQKFESIEPDQ 321
HAG RI WA+VKDI VAK M F+++S+I V+K+SQ AA+++ + D + ++ + D+
Sbjct: 182 HAGLRIWWALVKDIGVAKNMTKFIEISTISVSKDSQLIAAQVLNAVSDSCE-HDNSQYDE 358
Query: 322 LFFEYCKNILRERWEKLRRSIELRKVFTVAKY 353
F Y I+ +RW+ LR +E FT+ ++
Sbjct: 359 SFVMYSYYIMSQRWKPLRAVVEACDXFTLTQF 454
>AW759622
Length = 146
Score = 62.8 bits (151), Expect = 3e-10
Identities = 24/38 (63%), Positives = 32/38 (84%)
Frame = +3
Query: 226 EGKLVHDLAYYWPHYTPITHQANHDVMLFTLSKCTGHA 263
+G+ +HDLAYYWP YTPI+ ++HD+ LFT+SK TGHA
Sbjct: 33 QGQFIHDLAYYWPQYTPISSPSDHDLTLFTVSKTTGHA 146
>TC226060 similar to GB|AAP68293.1|31711874|BT008854 At1g80360 {Arabidopsis
thaliana;} , partial (89%)
Length = 1770
Score = 38.9 bits (89), Expect = 0.005
Identities = 29/77 (37%), Positives = 40/77 (51%), Gaps = 10/77 (12%)
Frame = +1
Query: 203 IEVVNYPNNPDGT-IKEPMVKSIVE------GKLVHDLAYYWPHYTPITH---QANHDVM 252
+ VVN P NP GT I EP++K I + LV D Y + Y + H + NH V
Sbjct: 679 VTVVN-PGNPSGTYIPEPLLKRISDLCKNAGSWLVVDNTYEYFMYDGLKHSCVEGNHIVN 855
Query: 253 LFTLSKCTGHAGSRIGW 269
+F+ SK G G R+G+
Sbjct: 856 VFSFSKAFGMMGWRVGY 906
>BU763775
Length = 437
Score = 33.9 bits (76), Expect = 0.15
Identities = 21/53 (39%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +2
Query: 374 LKCEEGIE-NAENYLGKLKIRGRGGVRFGAGSKHVRLSMIGTDDEFTELIKRL 425
LKCE + N L I GR G F A ++VRLS+I + D+F L +L
Sbjct: 2 LKCERQQDMNCYETLKAAGIIGRKGSNFSADERYVRLSLIKSQDDFEILTNKL 160
>TC219250 similar to UP|O82030 (O82030) Histidinol-phosphate aminotransferase
precursor , partial (87%)
Length = 1564
Score = 30.4 bits (67), Expect = 1.6
Identities = 28/100 (28%), Positives = 50/100 (50%), Gaps = 7/100 (7%)
Frame = +1
Query: 209 PNNPDGT-IKEPMVKSIVEGKLVHDLAYYWPHYTPITHQAN----HD--VMLFTLSKCTG 261
PNNPDG+ I + ++ I+E ++ L + ++ I + + HD ++L T SK G
Sbjct: 859 PNNPDGSIIDDEVLLKILELPILVILDEAYIEFSAIESRMSWVKKHDNLIVLRTFSKRAG 1038
Query: 262 HAGSRIGWAIVKDIEVAKKMVMFVQMSSIGVAKESQTRAA 301
AG R+G+ + + + + Q ++ VA E AA
Sbjct: 1039LAGLRVGYGAF-PLSIIEYLWRAKQPYNVSVAAEISACAA 1155
>TC230352
Length = 915
Score = 30.0 bits (66), Expect = 2.1
Identities = 17/74 (22%), Positives = 37/74 (49%)
Frame = -3
Query: 96 ELMSYLSDTSNVCWYMLPELKDAIERLHHVVRNAVTKDKYIVIGTGTTQLFMAALFALSP 155
E + +S TS +++P+L DA ++H++ + K +V+ QL ++ ++ P
Sbjct: 754 ESYTIISLTSIFTKHVIPQLFDATRTVYHIIFSQAIKSIQLVL-----QLLSRSILSMQP 590
Query: 156 SMTTSHPINVVSAT 169
S + V++ T
Sbjct: 589 KTCKSRKLEVMNKT 548
>TC231666 weakly similar to UP|A4E1_HUMAN (Q9UPM8) Adapter-related protein
complex 4 epsilon 1 subunit (Epsilon subunit of AP-4)
(AP-4 adapter complex epsilon subunit), partial (7%)
Length = 535
Score = 29.6 bits (65), Expect = 2.7
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +3
Query: 200 ETYIEVVNYPNNPDGTIKEPMVKSIVEGKLVHDLAYYWPHYTPITHQANHDVMLFTLSKC 259
ET ++N + P IKE +++ + L HD ++ + H +TH HD +L K
Sbjct: 246 ETLKRLLNDADTPKRKIKEYIIRLLYVEMLGHDASFGYIHAVKMTH---HDALLL---KR 407
Query: 260 TGH 262
TG+
Sbjct: 408 TGY 416
>TC225638 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
complete
Length = 742
Score = 28.9 bits (63), Expect = 4.7
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = -2
Query: 218 EPMVKSIVEGKLVHDLAYYW 237
+P++K ++E VHDL +YW
Sbjct: 402 DPLLKKLLERSNVHDLVFYW 343
>TC225636 similar to UP|R22B_ARATH (Q9M9W1) 60S ribosomal protein L22-2,
partial (98%)
Length = 444
Score = 28.9 bits (63), Expect = 4.7
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = -3
Query: 218 EPMVKSIVEGKLVHDLAYYW 237
+P++K ++E VHDL +YW
Sbjct: 208 DPLLKKLLERSNVHDLVFYW 149
>TC212806 weakly similar to UP|A4E1_HUMAN (Q9UPM8) Adapter-related protein
complex 4 epsilon 1 subunit (Epsilon subunit of AP-4)
(AP-4 adapter complex epsilon subunit), partial (6%)
Length = 402
Score = 28.1 bits (61), Expect = 8.0
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = +3
Query: 200 ETYIEVVNYPNNPDGTIKEPMVKSIVEGKLVHDLAYYWPHYTPITHQANHDVMLFTLSKC 259
ET +N + P IKE +++ + L HD ++ + H +TH HD +L K
Sbjct: 141 ETLKRRINDADTPKRKIKEYIIRLLYVEMLGHDASFGYIHAVKMTH---HDSLLL---KR 302
Query: 260 TGH 262
TG+
Sbjct: 303 TGY 311
>AW308849 weakly similar to SP|O49046|ARGI_ Arginase (EC 3.5.3.1). [Soybean]
{Glycine max}, partial (20%)
Length = 223
Score = 28.1 bits (61), Expect = 8.0
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = -2
Query: 136 IVIGTGTTQLFMAALFALSPSMTTSHPINVVSATPYYSEYKDQLDLFCSERF 187
+V+ T +L ++L +L S T+ ++ S T Y++ Y D D+F + F
Sbjct: 150 VVVATAPPELCTSSLLSLVFSRTS---VSAASITRYWALYSDSDDIFSAHSF 4
>TC230772 similar to UP|O24648 (O24648) Gibberellin 3 beta-hydroxylase
(2-oxoglutarate-dependent dioxygenase), partial (85%)
Length = 1264
Score = 28.1 bits (61), Expect = 8.0
Identities = 15/72 (20%), Positives = 30/72 (40%), Gaps = 12/72 (16%)
Frame = +3
Query: 185 ERFQWGGDAGEYDKNETYIEVVNYPNNP------------DGTIKEPMVKSIVEGKLVHD 232
E +W G G+++K +++ +YP P D T+ + ++ + G VH
Sbjct: 510 EDLKWAGSKGQFEKTCAALQLNSYPTCPDPDRAMGLAAHTDSTLLTILYQNNISGLQVHR 689
Query: 233 LAYYWPHYTPIT 244
W P++
Sbjct: 690 KGVGWVTVPPLS 725
>NP005385 DNA-directed RNA polymerase
Length = 2934
Score = 28.1 bits (61), Expect = 8.0
Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 7/70 (10%)
Frame = -1
Query: 74 PLVFGKYWKKKSEECMVVIKG---CELMSYLSDTSNVCWYMLPELKDAIE----RLHHVV 126
PL + W++ +V I+G CELMS N C + +L D + L HV+
Sbjct: 519 PLEVNRQWQR-----IVTIRGYLYCELMSISFKLLNFCLKHITKLSDGFQVFYRLLKHVI 355
Query: 127 RNAVTKDKYI 136
R V K+I
Sbjct: 354 RLPVFLVKFI 325
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.135 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,307,554
Number of Sequences: 63676
Number of extensions: 255668
Number of successful extensions: 1310
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1294
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1299
length of query: 430
length of database: 12,639,632
effective HSP length: 100
effective length of query: 330
effective length of database: 6,272,032
effective search space: 2069770560
effective search space used: 2069770560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0076a.12