BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0074.12
(369 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM885356 43 2e-04
NP595172 polyprotein [Glycine max] 37 0.019
TC213962 35 0.054
TC220401 34 0.12
CF922341 32 0.46
TC205857 similar to UP|Q9LVB9 (Q9LVB9) Emb|CAB83157.1, partial (... 31 1.0
TC205860 similar to UP|Q9LVB9 (Q9LVB9) Emb|CAB83157.1, partial (... 31 1.0
NP005385 DNA-directed RNA polymerase 30 1.3
TC225011 similar to UP|Q09083 (Q09083) Hydroxyproline-rich glyco... 30 2.3
BQ785893 similar to PIR|T10863|T108 extensin precursor - kidney ... 30 2.3
TC230959 similar to UP|Q9SPB4 (Q9SPB4) Non-phototropic hypocotyl... 30 2.3
BE058585 30 2.3
TC222563 similar to UP|Q9FS92 (Q9FS92) Outwardly rectifying pota... 29 3.0
CA784792 similar to PIR|T06782|T067 extensin - soybean, partial ... 29 3.0
TC222980 similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%) 29 3.0
BG238310 29 3.0
CD487321 29 3.9
TC224968 similar to UP|Q39835 (Q39835) Extensin, partial (44%) 28 5.0
TC225027 similar to UP|Q39835 (Q39835) Extensin, partial (49%) 28 5.0
TC206203 UP|Q99366 (Q99366) DNA-directed RNA polymerase (Fragme... 28 5.0
>BM885356
Length = 420
Score = 43.1 bits (100), Expect = 2e-04
Identities = 29/108 (26%), Positives = 45/108 (40%), Gaps = 5/108 (4%)
Frame = -3
Query: 23 YSPPSQPNTSPYYCHNVPSYYPQTHSSLQLSEFNGENPEYWLFMADCYFLQNPYPWETRF 82
+SP P + PS P L + F+G + W+F +F + P R
Sbjct: 322 FSPSQTQPEMPSFPAAGPSAAPH-RLKLDVPRFDGSDATGWIFKITQFFEYHTTPDHERL 146
Query: 83 QLLAIHLGGKALTWFRLSHQ--QIHSWEEFKVAFRLYFV---YNRPNG 125
+ + ++ G+AL WF+ H+ Q+ SW F A F Y P G
Sbjct: 145 TIASFYMEGQALAWFQWMHRNGQLSSWPAFLHALHSRFASTSYEDPTG 2
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 36.6 bits (83), Expect = 0.019
Identities = 19/71 (26%), Positives = 33/71 (45%), Gaps = 2/71 (2%)
Frame = +1
Query: 50 LQLSEFNGENPEYWLFMADCYFLQNPYPWETRFQLLAIHLGGKALTWFRL--SHQQIHSW 107
L F+G+N W+F A+ +F P R + ++HL + W+++ + SW
Sbjct: 298 LDFPRFDGKNVMDWIFKAEQFFDYYATPDADRLIIASVHLDQDVVPWYQMLQKTEPFSSW 477
Query: 108 EEFKVAFRLYF 118
+ F A L F
Sbjct: 478 QAFTRALELDF 510
>TC213962
Length = 799
Score = 35.0 bits (79), Expect = 0.054
Identities = 18/71 (25%), Positives = 35/71 (48%), Gaps = 2/71 (2%)
Frame = -2
Query: 30 NTSPYYCHNVPSYYPQ--THSSLQLSEFNGENPEYWLFMADCYFLQNPYPWETRFQLLAI 87
N+S + +P+ +P TH L++ F+G++P W+F + P + +
Sbjct: 441 NSSHQHFAQIPTIFPHPCTHMKLEVPWFDGQDPLGWIFKITQFSNYQGIPNVEHLIVGSF 262
Query: 88 HLGGKALTWFR 98
++ G AL W+R
Sbjct: 261 NMEGPALCWYR 229
>TC220401
Length = 1286
Score = 33.9 bits (76), Expect = 0.12
Identities = 20/81 (24%), Positives = 35/81 (42%)
Frame = +1
Query: 24 SPPSQPNTSPYYCHNVPSYYPQTHSSLQLSEFNGENPEYWLFMADCYFLQNPYPWETRFQ 83
SP SQP+ SP + + H L FNG +P W+F +F +
Sbjct: 199 SPSSQPSPSPIH---------RPHMKLDAPRFNGHDPLGWIFKISQFFDYQTILEQEPLT 351
Query: 84 LLAIHLGGKALTWFRLSHQQI 104
+ + ++ G AL ++ +Q+
Sbjct: 352 VASFYMDGSALH*YQWMSRQV 414
>CF922341
Length = 675
Score = 32.0 bits (71), Expect = 0.46
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +1
Query: 89 LGGKALTWF-RLSHQQIHSWEEFKVAFRLYFVYN 121
L G A+TW+ L ++HSW++ VAF + YN
Sbjct: 544 LTGVAVTWYTNLEPSRVHSWKDLMVAFVRQYQYN 645
>TC205857 similar to UP|Q9LVB9 (Q9LVB9) Emb|CAB83157.1, partial (28%)
Length = 1056
Score = 30.8 bits (68), Expect = 1.0
Identities = 18/70 (25%), Positives = 35/70 (49%)
Frame = -3
Query: 169 PFEASSDVHLLPEVVSRVELGSAETEIESHESSTSEDSSLITTPVDSTPYATRSDAITPD 228
PFE ++ + + S E +IES E + +D SL++ STP + + + P
Sbjct: 289 PFECVGELEDVEDEASSANSDEEEEQIESSELNPMDDESLLS----STPSSLITQLVFPT 122
Query: 229 VNKTFVLKTT 238
++ + +L T+
Sbjct: 121 LSPSMILFTS 92
>TC205860 similar to UP|Q9LVB9 (Q9LVB9) Emb|CAB83157.1, partial (10%)
Length = 441
Score = 30.8 bits (68), Expect = 1.0
Identities = 18/70 (25%), Positives = 35/70 (49%)
Frame = -1
Query: 169 PFEASSDVHLLPEVVSRVELGSAETEIESHESSTSEDSSLITTPVDSTPYATRSDAITPD 228
PFE ++ + + S E +IES E + +D SL++ STP + + + P
Sbjct: 306 PFECVGELEDVEDEASSANSDEEEEQIESSELNPMDDESLLS----STPSSLITQLVFPT 139
Query: 229 VNKTFVLKTT 238
++ + +L T+
Sbjct: 138 LSPSMILFTS 109
>NP005385 DNA-directed RNA polymerase
Length = 2934
Score = 30.4 bits (67), Expect = 1.3
Identities = 23/60 (38%), Positives = 31/60 (51%), Gaps = 10/60 (16%)
Frame = +1
Query: 13 SAQRDPRSIFYSP------PSQPN---TSPYYCHNVPSYYPQTHSSLQLSEFN-GENPEY 62
SA+ P S+ YSP P+ PN TSP Y PSY P + + S +N G +P+Y
Sbjct: 2644 SAKYSP-SLAYSPSSPRLSPTSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYNSGVSPDY 2820
Score = 30.4 bits (67), Expect = 1.3
Identities = 21/56 (37%), Positives = 27/56 (47%), Gaps = 3/56 (5%)
Frame = +1
Query: 10 SILSAQRDPRSIFYSP--PSQPNTSPYYCHNVPSYYPQTHSSLQLS-EFNGENPEY 62
S+ S P S YSP PS TSP Y PSY P + S S +N ++ +Y
Sbjct: 2488 SLTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPTSPSYNPQSAKY 2655
>TC225011 similar to UP|Q09083 (Q09083) Hydroxyproline-rich glycoprotein
precursor, partial (20%)
Length = 964
Score = 29.6 bits (65), Expect = 2.3
Identities = 12/38 (31%), Positives = 19/38 (49%)
Frame = +2
Query: 18 PRSIFYSPPSQPNTSPYYCHNVPSYYPQTHSSLQLSEF 55
P +++SPP P PY + P Y H+S + E+
Sbjct: 476 PHPVYHSPPPPPYKKPYIYASPPPPYHY*HTSYRYEEY 589
>BQ785893 similar to PIR|T10863|T108 extensin precursor - kidney bean,
partial (17%)
Length = 429
Score = 29.6 bits (65), Expect = 2.3
Identities = 12/38 (31%), Positives = 19/38 (49%)
Frame = +3
Query: 18 PRSIFYSPPSQPNTSPYYCHNVPSYYPQTHSSLQLSEF 55
P +++SPP P PY + P Y H+S + E+
Sbjct: 240 PHPVYHSPPPPPYKKPYIYASPPPPYHY*HTSYRYEEY 353
Score = 28.1 bits (61), Expect = 6.6
Identities = 10/24 (41%), Positives = 14/24 (57%)
Frame = +3
Query: 22 FYSPPSQPNTSPYYCHNVPSYYPQ 45
++SPP P PYY H+ P P+
Sbjct: 78 YHSPPPPPKKKPYYYHSPPPPPPK 149
>TC230959 similar to UP|Q9SPB4 (Q9SPB4) Non-phototropic hypocotyl 3, partial
(26%)
Length = 944
Score = 29.6 bits (65), Expect = 2.3
Identities = 14/35 (40%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Frame = +1
Query: 256 KFVQKNNEAQ-FEKHFDKLLMQFHNSSTTVDVQKI 289
K+++ N+ + +KHFDKLL Q H S+ T +K+
Sbjct: 484 KYLELQNDMENLQKHFDKLLKQKHTSAWTSGWKKL 588
>BE058585
Length = 320
Score = 29.6 bits (65), Expect = 2.3
Identities = 12/38 (31%), Positives = 19/38 (49%)
Frame = +3
Query: 18 PRSIFYSPPSQPNTSPYYCHNVPSYYPQTHSSLQLSEF 55
P +++SPP P PY + P Y H+S + E+
Sbjct: 24 PHPVYHSPPPPPYKKPYIYASPPPPYHY*HTSYRYEEY 137
>TC222563 similar to UP|Q9FS92 (Q9FS92) Outwardly rectifying potassium
channel, partial (22%)
Length = 557
Score = 29.3 bits (64), Expect = 3.0
Identities = 39/137 (28%), Positives = 55/137 (39%), Gaps = 5/137 (3%)
Frame = +1
Query: 141 VEIQRSSEQVSPHRVVFSETTLEDGAQLPFEASSDVHLLPEVVSRVELGSAET-EIESHE 199
+ I+ E P V+ + + D QL F H + E V E G+ ET +
Sbjct: 133 IVIRLHEEFFLPGEVIMEQGNVVD--QLYFVC----HGVLEEVGTAEHGTEETVSLLQPN 294
Query: 200 SSTSEDSSLITTPVDSTPYATRSDAITP--DVNKTFVLKTTDLVLRNPRQVFGNLLEGK- 256
SS E S L P PY R ++ ++K D+ + R+V NLLEGK
Sbjct: 295 SSFGEISILCNIP---QPYTDRVCELSRLLRLDKQSFTNILDIYFYDGRKVLNNLLEGKE 465
Query: 257 -FVQKNNEAQFEKHFDK 272
F K E+ H K
Sbjct: 466 SFRDKQLESDITFHIGK 516
>CA784792 similar to PIR|T06782|T067 extensin - soybean, partial (11%)
Length = 435
Score = 29.3 bits (64), Expect = 3.0
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +3
Query: 22 FYSPPSQPNTSPYYCHNVPSYYPQTH 47
++SPP P PYY H+ P Y + +
Sbjct: 54 YHSPPPPPKKKPYYYHSPPPPYKKPY 131
>TC222980 similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 662
Score = 29.3 bits (64), Expect = 3.0
Identities = 20/91 (21%), Positives = 38/91 (40%), Gaps = 2/91 (2%)
Frame = +3
Query: 30 NTSPYYCHNVPSYYPQTHSSLQLSEFNGENPEYWLFMADCYFLQNPYPWETRFQLLAIHL 89
N P+ NV +P +G + W+F A+ +F + P E R + +IHL
Sbjct: 315 NNHPFQVRNVKIDFPI---------LDGSDVLQWIFKAEQFFNYHKTPGEQRLIIASIHL 467
Query: 90 GGKALTWFRLSHQQ--IHSWEEFKVAFRLYF 118
+ + +++ ++ +W F A F
Sbjct: 468 DKEVVPCYQMMTRENSFKTWIAFTRALETEF 560
>BG238310
Length = 353
Score = 29.3 bits (64), Expect = 3.0
Identities = 26/85 (30%), Positives = 38/85 (44%)
Frame = +2
Query: 127 STTTVKYGFEKSSLVEIQRSSEQVSPHRVVFSETTLEDGAQLPFEASSDVHLLPEVVSRV 186
S+T + GF++ S + + SP VVF TLE L AS+ V R
Sbjct: 65 SSTMAEVGFQERSSWRVGLRARYSSPDSVVF---TLESNLSLFSYASAS-------VDRC 214
Query: 187 ELGSAETEIESHESSTSEDSSLITT 211
S + +S S S DS+L++T
Sbjct: 215 SFSSDSHDHDSFVSEISLDSTLLST 289
>CD487321
Length = 638
Score = 28.9 bits (63), Expect = 3.9
Identities = 20/50 (40%), Positives = 26/50 (52%)
Frame = -3
Query: 159 ETTLEDGAQLPFEASSDVHLLPEVVSRVELGSAETEIESHESSTSEDSSL 208
E T E + EA++D HLL V R E+ SHES+ S+ SSL
Sbjct: 462 EGTCEQASLKLTEAAADFHLLHTEVRR-----KNEEVGSHESTLSKRSSL 328
>TC224968 similar to UP|Q39835 (Q39835) Extensin, partial (44%)
Length = 681
Score = 28.5 bits (62), Expect = 5.0
Identities = 10/23 (43%), Positives = 12/23 (51%)
Frame = +3
Query: 25 PPSQPNTSPYYCHNVPSYYPQTH 47
PP P PYY H+ P +P H
Sbjct: 477 PPPPPKKKPYYYHSPPPPHPYPH 545
>TC225027 similar to UP|Q39835 (Q39835) Extensin, partial (49%)
Length = 747
Score = 28.5 bits (62), Expect = 5.0
Identities = 10/23 (43%), Positives = 12/23 (51%)
Frame = +2
Query: 25 PPSQPNTSPYYCHNVPSYYPQTH 47
PP P PYY H+ P +P H
Sbjct: 515 PPPPPKKKPYYYHSPPPPHPYPH 583
>TC206203 UP|Q99366 (Q99366) DNA-directed RNA polymerase (Fragment) , partial
(96%)
Length = 2604
Score = 28.5 bits (62), Expect = 5.0
Identities = 17/43 (39%), Positives = 23/43 (52%), Gaps = 3/43 (6%)
Frame = +1
Query: 23 YSP--PSQPNTSPYYCHNVPSYYPQTHSSLQLSEFN-GENPEY 62
YSP P+ TSP Y PSY P + + S +N G +P+Y
Sbjct: 1711 YSPTSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYNSGVSPDY 1839
Score = 28.1 bits (61), Expect = 6.6
Identities = 16/31 (51%), Positives = 17/31 (54%), Gaps = 2/31 (6%)
Frame = +1
Query: 18 PRSIFYSP--PSQPNTSPYYCHNVPSYYPQT 46
P S YSP PS TSP Y PSY PQ+
Sbjct: 1555 PTSPGYSPTSPSYSPTSPSYSPTSPSYNPQS 1647
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,540,740
Number of Sequences: 63676
Number of extensions: 274407
Number of successful extensions: 1793
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 1612
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1763
length of query: 369
length of database: 12,639,632
effective HSP length: 99
effective length of query: 270
effective length of database: 6,335,708
effective search space: 1710641160
effective search space used: 1710641160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0074.12