BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0072a.1
(224 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC209016 similar to UP|Q7XZT4 (Q7XZT4) RPA 32kDa, complete 343 4e-95
TC233567 weakly similar to UP|Q7XZT4 (Q7XZT4) RPA 32kDa, partial... 117 3e-27
CO983565 51 4e-07
TC221851 weakly similar to UP|Q9M208 (Q9M208) Enoyl-CoA-hydratas... 30 1.1
TC228205 similar to UP|Q38820 (Q38820) Inner mitochondrial membr... 29 1.5
TC222136 similar to UP|HMN1_DROME (P22807) Homeobox protein slou... 29 2.0
TC224992 28 2.5
TC213517 similar to UP|RK32_LOTJA (Q9BBP5) Chloroplast 50S ribos... 28 2.5
TC228108 similar to UP|Q6NQG5 (Q6NQG5) At1g49170, partial (79%) 28 4.3
TC224972 similar to UP|Q9M084 (Q9M084) Aspartate--tRNA ligase-li... 28 4.3
TC221817 similar to GB|AAP37839.1|30725634|BT008480 At5g64750 {A... 28 4.3
TC225826 homologue to UP|PDX1_PHAVU (Q9FT25) Probable pyridoxin ... 27 5.7
TC204440 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehy... 27 5.7
TC224637 similar to UP|Q8LKG1 (Q8LKG1) Drm3, partial (98%) 27 5.7
TC233358 27 7.4
TC210010 27 9.7
TC204437 homologue to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol de... 27 9.7
TC229113 similar to UP|Q9ZVK5 (Q9ZVK5) Expressed protein, partia... 27 9.7
CF921606 27 9.7
TC234084 similar to UP|Q9FNG9 (Q9FNG9) Gb|AAF02137.1, partial (16%) 27 9.7
>TC209016 similar to UP|Q7XZT4 (Q7XZT4) RPA 32kDa, complete
Length = 990
Score = 343 bits (880), Expect = 4e-95
Identities = 170/229 (74%), Positives = 198/229 (86%), Gaps = 5/229 (2%)
Frame = +1
Query: 1 MFSVTQFDSSSG--GGGFTSTQ---LNDSSPAPQKGRESQGLVPVTVKQINEASQSGDEK 55
MFS +QFD+++ GGGFTS+Q LNDSSPAP K RE+QGLVPVTVKQI+EASQSGDEK
Sbjct: 52 MFSGSQFDATTAFSGGGFTSSQPSTLNDSSPAPSKSRETQGLVPVTVKQISEASQSGDEK 231
Query: 56 SSFVINGVELTNVTLVGMVFEKAERNTDVNFVLDDGTGRIKCRRWINDAFDTQEVEEIMN 115
S+FVINGV+L NVTL+GMVFEK ERNTDVNFVLDDGTGRIKCRRW+N+AFDT+E+E +MN
Sbjct: 232 SNFVINGVDLNNVTLLGMVFEKVERNTDVNFVLDDGTGRIKCRRWVNEAFDTKEMEAVMN 411
Query: 116 GMYVRVNGHLKSFQGVRQVAAFSVRPVVNFDEIPFHFIDCIHHHLRSKLKMEGTISTDPP 175
GMYVRV GHLKSFQGV+Q+ FS RPV NFDEIPFHFIDCIH+HLRSK+KMEG I++ P
Sbjct: 412 GMYVRVYGHLKSFQGVKQLVTFSARPVTNFDEIPFHFIDCIHNHLRSKIKMEG-ITSSNP 588
Query: 176 ASDSSLKTPVRNTSNGSQAPSSTPAYAQYGVDGLKDCDKLVIDYLQQHS 224
+S SSL TP ++ NGSQAPSS P AQ+ VDGLK DKL++DYL+QHS
Sbjct: 589 SSGSSLNTPGKSAPNGSQAPSSNPVSAQHSVDGLKGIDKLIMDYLEQHS 735
>TC233567 weakly similar to UP|Q7XZT4 (Q7XZT4) RPA 32kDa, partial (27%)
Length = 473
Score = 117 bits (294), Expect = 3e-27
Identities = 61/109 (55%), Positives = 81/109 (73%), Gaps = 5/109 (4%)
Frame = +2
Query: 3 SVTQFDSSSG--GGGFT---STQLNDSSPAPQKGRESQGLVPVTVKQINEASQSGDEKSS 57
S +QFD ++ GGGF +TQ DSS P K R++Q L+P+TVKQI +ASQS D+K +
Sbjct: 146 SASQFDGNAAFSGGGFMPSQTTQGPDSSFVPSKNRDAQSLLPLTVKQIYDASQSSDDKIN 325
Query: 58 FVINGVELTNVTLVGMVFEKAERNTDVNFVLDDGTGRIKCRRWINDAFD 106
+I+GV++TNVTLVG V KA R TDV FVLDDGTGRI+C +W+++A D
Sbjct: 326 LIIDGVDVTNVTLVGRVSNKAGRITDVTFVLDDGTGRIECNKWLHEAVD 472
>CO983565
Length = 753
Score = 51.2 bits (121), Expect = 4e-07
Identities = 37/101 (36%), Positives = 50/101 (48%), Gaps = 3/101 (2%)
Frame = -3
Query: 123 GHLKSFQGVRQVAAFSVRPVVNFDEIPFHFIDCIHHHL-RSKLKMEGTISTDPPASDSSL 181
G LK FQG R + FS RPV +F+EI HF DCI+ HL SKL+ T P S
Sbjct: 751 GQLKHFQGKRTLNVFSFRPVTDFNEIASHFTDCIYVHLYNSKLR------TSVPNQQHSS 590
Query: 182 KTPVRNTSNGSQAPSSTPAYAQYGVDGLK--DCDKLVIDYL 220
P +Q T ++ V+G K + +V+D+L
Sbjct: 589 PIP-PTIGYQAQVVPPTNQFSDQHVNGQKGVTVEAMVLDFL 470
>TC221851 weakly similar to UP|Q9M208 (Q9M208) Enoyl-CoA-hydratase-like
protein, partial (23%)
Length = 643
Score = 29.6 bits (65), Expect = 1.1
Identities = 17/67 (25%), Positives = 33/67 (48%), Gaps = 8/67 (11%)
Frame = +1
Query: 60 INGVELTNVTLVGMVFEKAERNTDVNFVLDDGTGRIKCR--------RWINDAFDTQEVE 111
+N + + V + +++ E N+D+ FVL G+GR C + +N+ +T E E
Sbjct: 322 LNSLNASMVARLKRLYDSWEENSDIGFVLMKGSGRAFCSGADVVRLYQSLNEG-NTDEAE 498
Query: 112 EIMNGMY 118
+ +Y
Sbjct: 499 QFFKTLY 519
>TC228205 similar to UP|Q38820 (Q38820) Inner mitochondrial membrane protein
(Mitochondrial inner membrane translocase TM23-2) (Inner
mitochondrial membrane protein; 26940-26374), partial
(73%)
Length = 528
Score = 29.3 bits (64), Expect = 1.5
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +1
Query: 162 SKLKMEGTISTDPPASDSSLKTPVRNTSNGSQAPSSTPAYA 202
SK + S+ PP S SS + P + G++ STPA A
Sbjct: 136 SKSPFATSTSSQPPRSTSSSRRPAASADPGARTSPSTPAAA 258
>TC222136 similar to UP|HMN1_DROME (P22807) Homeobox protein slou (S59/2)
(Slouch protein) (Homeobox protein NK-1), partial (3%)
Length = 562
Score = 28.9 bits (63), Expect = 2.0
Identities = 15/39 (38%), Positives = 22/39 (55%)
Frame = +3
Query: 162 SKLKMEGTISTDPPASDSSLKTPVRNTSNGSQAPSSTPA 200
S L++ + AS S T + S+GS++PSSTPA
Sbjct: 411 SPLRLRSAPTPSTSASPQSPPTSPSSASSGSRSPSSTPA 527
>TC224992
Length = 615
Score = 28.5 bits (62), Expect = 2.5
Identities = 17/46 (36%), Positives = 23/46 (49%), Gaps = 1/46 (2%)
Frame = -3
Query: 152 FIDCIHHHLRSKLKMEGTISTDPPASDSSLKTPVR-NTSNGSQAPS 196
FI IHHHL+S+ + + P S P R N + GS+ PS
Sbjct: 400 FIHLIHHHLKSQRPSISSKT*FPQLDTPSYFMPTRANKTRGSKCPS 263
>TC213517 similar to UP|RK32_LOTJA (Q9BBP5) Chloroplast 50S ribosomal protein
L32, complete
Length = 789
Score = 28.5 bits (62), Expect = 2.5
Identities = 14/37 (37%), Positives = 23/37 (61%), Gaps = 1/37 (2%)
Frame = +1
Query: 52 GDEKSSFVIN-GVELTNVTLVGMVFEKAERNTDVNFV 87
G +K+ FV N V L ++ V +F +RNT++NF+
Sbjct: 352 GIQKAFFVTNTNVRLISIN*VQRIFLNTKRNTNINFI 462
>TC228108 similar to UP|Q6NQG5 (Q6NQG5) At1g49170, partial (79%)
Length = 675
Score = 27.7 bits (60), Expect = 4.3
Identities = 12/45 (26%), Positives = 26/45 (57%)
Frame = -3
Query: 42 VKQINEASQSGDEKSSFVINGVELTNVTLVGMVFEKAERNTDVNF 86
+KQI+ ++ + +S ++ G + +L ++ EKA+R T +F
Sbjct: 652 IKQIHHSTLQRCQSNSSLLTGK*IITYSLAALIVEKAQRKTRFHF 518
>TC224972 similar to UP|Q9M084 (Q9M084) Aspartate--tRNA ligase-like protein,
partial (49%)
Length = 1255
Score = 27.7 bits (60), Expect = 4.3
Identities = 13/32 (40%), Positives = 20/32 (61%)
Frame = -1
Query: 189 SNGSQAPSSTPAYAQYGVDGLKDCDKLVIDYL 220
SNG P+++ ++ Q V +K CDK IDY+
Sbjct: 1195 SNGY*DPTASRSFLQSLVQTVKHCDKQSIDYI 1100
>TC221817 similar to GB|AAP37839.1|30725634|BT008480 At5g64750 {Arabidopsis
thaliana;} , partial (24%)
Length = 1008
Score = 27.7 bits (60), Expect = 4.3
Identities = 18/49 (36%), Positives = 26/49 (52%), Gaps = 1/49 (2%)
Frame = +1
Query: 158 HHLRSKLKMEGTISTDPPASDSSLKTPVRN-TSNGSQAPSSTPAYAQYG 205
HHL+S L + PPA+ SSL +P +N TS S + P ++ G
Sbjct: 805 HHLQSSLPL-------PPATSSSLTSPPQNITSLSSLYSAQLPPWSASG 930
>TC225826 homologue to UP|PDX1_PHAVU (Q9FT25) Probable pyridoxin biosynthesis
protein PDX1 (pvPDX1), partial (96%)
Length = 1482
Score = 27.3 bits (59), Expect = 5.7
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = +2
Query: 170 ISTDPPASDSSLKTPVRNTSNGSQAPSSTPAYAQYGV 206
+ST PPA TP S+ + A SS PA+++ +
Sbjct: 785 LSTSPPAESPPPPTPRS*CSSAATASSSAPAFSRAAI 895
>TC204440 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehydrogenase
(CAD) , partial (97%)
Length = 1481
Score = 27.3 bits (59), Expect = 5.7
Identities = 22/77 (28%), Positives = 37/77 (47%), Gaps = 2/77 (2%)
Frame = +3
Query: 15 GFTSTQLNDSSPAPQKGRES-QGLVPVTVKQINEASQSGDEKSSFVINGVELTNVTLVGM 73
G +T L SP GR+S G ++K+ E + EK + + +E+ N+ +
Sbjct: 882 GVINTPLQFVSPMVMLGRKSITGSFIGSMKETEEMLEFWKEKG--LSSMIEMVNMDYINK 1055
Query: 74 VFEKAERN-TDVNFVLD 89
FE+ E+N FV+D
Sbjct: 1056AFERLEKNDVRYRFVVD 1106
>TC224637 similar to UP|Q8LKG1 (Q8LKG1) Drm3, partial (98%)
Length = 673
Score = 27.3 bits (59), Expect = 5.7
Identities = 19/78 (24%), Positives = 30/78 (38%)
Frame = -3
Query: 118 YVRVNGHLKSFQGVRQVAAFSVRPVVNFDEIPFHFIDCIHHHLRSKLKMEGTISTDPPAS 177
Y+R H ++ P +N E+P D IH H + KM + S
Sbjct: 572 YLRFGNHYAAYLSKDLKKLNQKLPRMNLAELPCRHSDIIHAH-KD*TKMNTAKAKKAATS 396
Query: 178 DSSLKTPVRNTSNGSQAP 195
+ +R+TS G + P
Sbjct: 395 SAFRYEKMRHTSKGEEGP 342
>TC233358
Length = 932
Score = 26.9 bits (58), Expect = 7.4
Identities = 19/54 (35%), Positives = 24/54 (44%)
Frame = +3
Query: 154 DCIHHHLRSKLKMEGTISTDPPASDSSLKTPVRNTSNGSQAPSSTPAYAQYGVD 207
D I H LRS + S + S+ + N GSQAP S PAY + D
Sbjct: 63 DLIDHFLRSNDSLASLHSLSHIIENPSIT--MNNYPFGSQAPKSFPAYPRTEFD 218
>TC210010
Length = 682
Score = 26.6 bits (57), Expect = 9.7
Identities = 11/44 (25%), Positives = 21/44 (47%)
Frame = -2
Query: 156 IHHHLRSKLKMEGTISTDPPASDSSLKTPVRNTSNGSQAPSSTP 199
+H+H + DPP + + +S+GS +P++TP
Sbjct: 141 LHNHPFNPQASSSNFDGDPPCKTAGKTNHLTRSSSGSFSPTTTP 10
>TC204437 homologue to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehydrogenase
(CAD) , partial (49%)
Length = 844
Score = 26.6 bits (57), Expect = 9.7
Identities = 22/77 (28%), Positives = 36/77 (46%), Gaps = 2/77 (2%)
Frame = +3
Query: 15 GFTSTQLNDSSPAPQKGRES-QGLVPVTVKQINEASQSGDEKSSFVINGVELTNVTLVGM 73
G +T L SP GR S G ++K+ E + EK + + +E+ N+ +
Sbjct: 291 GVINTPLQFVSPMVMLGRRSITGSFIGSMKETEEMLEFWKEKG--LSSMIEVVNMDYINK 464
Query: 74 VFEKAERN-TDVNFVLD 89
FE+ E+N FV+D
Sbjct: 465 AFERLEKNDVRYRFVVD 515
>TC229113 similar to UP|Q9ZVK5 (Q9ZVK5) Expressed protein, partial (83%)
Length = 598
Score = 26.6 bits (57), Expect = 9.7
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = +2
Query: 98 RRWINDAFDTQEVEEIMNGMYVRVNGHLKSFQGVRQVAAFSVRPV 142
R W DA D + V E+ + ++ H K F +RQ SV PV
Sbjct: 260 REWYGDAVDVKAVGEMRRPVDLKEMKHFKDFALLRQ-PRLSVVPV 391
>CF921606
Length = 518
Score = 26.6 bits (57), Expect = 9.7
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +3
Query: 11 SGGGGFTSTQLNDSSPAPQKGRESQGLVPVTVKQ 44
+GGGG +Q N P P ++ +G P+ K+
Sbjct: 39 TGGGGKAPSQKNQPPPPPHPQKKKRGPPPIKKKR 140
>TC234084 similar to UP|Q9FNG9 (Q9FNG9) Gb|AAF02137.1, partial (16%)
Length = 418
Score = 26.6 bits (57), Expect = 9.7
Identities = 14/39 (35%), Positives = 19/39 (47%)
Frame = +1
Query: 179 SSLKTPVRNTSNGSQAPSSTPAYAQYGVDGLKDCDKLVI 217
SSLKTP T++ S P + P A ++ C L I
Sbjct: 289 SSLKTPSATTTSPSSPPETKPLNAT*TLNSSVSCSSLAI 405
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.315 0.132 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,645,067
Number of Sequences: 63676
Number of extensions: 102647
Number of successful extensions: 853
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 839
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 852
length of query: 224
length of database: 12,639,632
effective HSP length: 94
effective length of query: 130
effective length of database: 6,654,088
effective search space: 865031440
effective search space used: 865031440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0072a.1