BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0050.12
(143 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC214062 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), ... 102 6e-23
TC214133 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), ... 102 8e-23
TC205044 similar to UP|O24651 (O24651) Cytochrome b5 (Fragment),... 100 3e-22
TC205043 similar to UP|O24651 (O24651) Cytochrome b5 (Fragment),... 100 3e-22
TC204407 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), ... 98 1e-21
TC204409 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), ... 94 2e-20
TC226039 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), ... 94 3e-20
TC226040 similar to UP|CYB5_BRAOL (P40934) Cytochrome b5, partia... 91 1e-19
BG405226 similar to GP|2647949|emb cytochrome b5 {Olea europaea}... 68 3e-17
BE330689 weakly similar to GP|2695711|emb cytochome b5 {Olea eur... 84 3e-17
BE059104 74 2e-14
BE210560 weakly similar to SP|Q42342|CY51_ Cytochrome b5 isoform... 71 2e-13
TC205882 UP|Q9SYR0 (Q9SYR0) Nitrate reductase (Fragment), complete 70 3e-13
BE802874 70 3e-13
TC207476 similar to UP|Q6ZA21 (Q6ZA21) Cytochrome b5-like, parti... 60 4e-10
BI970294 58 1e-09
TC207475 similar to UP|Q6ZA21 (Q6ZA21) Cytochrome b5-like, parti... 57 3e-09
TC206988 similar to GB|AAQ62405.1|34146794|BT010404 At5g09680 {A... 52 7e-08
TC231075 weakly similar to UP|Q8LB96 (Q8LB96) Delta-8 sphingolip... 49 1e-06
TC209257 similar to UP|Q8LB96 (Q8LB96) Delta-8 sphingolipid desa... 42 1e-04
>TC214062 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), complete
Length = 768
Score = 102 bits (254), Expect = 6e-23
Identities = 53/142 (37%), Positives = 84/142 (58%), Gaps = 4/142 (2%)
Frame = +2
Query: 2 AERRVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAV 61
++R++ T +VA+H KDCW++I+G+V DVT F+ +HPGGD+VLL GKD T +F+ V
Sbjct: 116 SDRKLHTFEEVAKHNQTKDCWLIISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDV 295
Query: 62 GHSKEAQNLVLKYQVGILQGATVQEIDVVHKEPNSKQ----EMSAFVIKEDAESKSVALF 117
GHS A++++ KY +G + +TV + + P Q + FVIK +
Sbjct: 296 GHSDSARDMMEKYYIGEIDASTV-PLKRTYIPPQQAQYNPDKTPEFVIK---------IL 445
Query: 118 EFFVPLVVATLYFGYRCLTRTD 139
+F VPL++ L F R T+ +
Sbjct: 446 QFLVPLLILGLAFVVRHYTKKE 511
>TC214133 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), complete
Length = 730
Score = 102 bits (253), Expect = 8e-23
Identities = 53/142 (37%), Positives = 84/142 (58%), Gaps = 4/142 (2%)
Frame = +2
Query: 2 AERRVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAV 61
++R++ T +VA+H KDCW++I+G+V DVT F+ +HPGGD+VLL GKD T +F+ V
Sbjct: 104 SDRKLHTFEEVAKHDQTKDCWLIISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDV 283
Query: 62 GHSKEAQNLVLKYQVGILQGATVQEIDVVHKEPNSKQ----EMSAFVIKEDAESKSVALF 117
GHS A++++ KY +G + +TV + + P Q + FVIK +
Sbjct: 284 GHSDSARDMMEKYYIGEIDSSTV-PLKRTYIPPQQAQYNPDKTPEFVIK---------IL 433
Query: 118 EFFVPLVVATLYFGYRCLTRTD 139
+F VPL++ L F R T+ +
Sbjct: 434 QFLVPLLILGLAFVVRHYTKKE 499
>TC205044 similar to UP|O24651 (O24651) Cytochrome b5 (Fragment), partial
(97%)
Length = 833
Score = 100 bits (248), Expect = 3e-22
Identities = 52/136 (38%), Positives = 84/136 (61%), Gaps = 2/136 (1%)
Frame = +2
Query: 5 RVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGHS 64
+VFTL++V+QH + KDCW+VI+G+V +VTKFL +HPGGD+VLL GKD T +F+ +GHS
Sbjct: 155 KVFTLAEVSQHNNAKDCWLVIHGKVYNVTKFLEDHPGGDEVLLSSTGKDATNDFEDIGHS 334
Query: 65 KEAQNLVLKYQVGILQGATV--QEIDVVHKEPNSKQEMSAFVIKEDAESKSVALFEFFVP 122
A ++ ++ VG + +T+ + K+P+ Q+ + I + + +F VP
Sbjct: 335 TSAVAMMDEFYVGDIDTSTIPSKVKYTPPKQPHYNQDKTPEFI--------IRILQFLVP 490
Query: 123 LVVATLYFGYRCLTRT 138
L + L G R T++
Sbjct: 491 LFILGLAVGIRFYTKS 538
>TC205043 similar to UP|O24651 (O24651) Cytochrome b5 (Fragment), partial
(97%)
Length = 733
Score = 100 bits (248), Expect = 3e-22
Identities = 53/137 (38%), Positives = 86/137 (62%), Gaps = 3/137 (2%)
Frame = +3
Query: 5 RVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGHS 64
+VF+L++V+QH + KDCW+VI+G+V +VTKFL +HPGGD+VLL GKD T +F+ +GHS
Sbjct: 63 KVFSLAEVSQHNNAKDCWLVIHGKVYNVTKFLEDHPGGDEVLLSSTGKDATNDFEDIGHS 242
Query: 65 KEAQNLVLKYQVGILQGATV--QEIDVVHKEPNSKQE-MSAFVIKEDAESKSVALFEFFV 121
A ++ ++ VG + +T+ + K+P+ Q+ M F+I+ + +F V
Sbjct: 243 TSAVAMMDEFYVGDIDTSTIPSKVKYTPPKQPHYNQDKMPEFIIR---------ILQFLV 395
Query: 122 PLVVATLYFGYRCLTRT 138
PL + L G R T++
Sbjct: 396 PLFILGLAVGIRFYTKS 446
>TC204407 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), partial
(78%)
Length = 857
Score = 98.2 bits (243), Expect = 1e-21
Identities = 52/132 (39%), Positives = 76/132 (57%), Gaps = 6/132 (4%)
Frame = +3
Query: 5 RVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGHS 64
+ T +VA+H + KDCW++ING+V D+T FL EHPGGD+VLL GKD T +F+ VGHS
Sbjct: 162 KTLTFEEVAKHNNKKDCWIIINGKVYDITPFLDEHPGGDEVLLTSTGKDATIDFEDVGHS 341
Query: 65 KEAQNLVLKYQVGILQGATVQEIDVVHKEPNSKQ------EMSAFVIKEDAESKSVALFE 118
A ++ KY +G + +T+ V H P Q + S FV+K + +
Sbjct: 342 DSAIEMMEKYFIGKVDTSTLPP-KVSHSLPQPTQTHGAGNQSSGFVVK---------ILQ 491
Query: 119 FFVPLVVATLYF 130
F +PL++ L F
Sbjct: 492 FLLPLLILGLAF 527
>TC204409 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), partial
(78%)
Length = 795
Score = 94.4 bits (233), Expect = 2e-20
Identities = 51/129 (39%), Positives = 73/129 (56%), Gaps = 6/129 (4%)
Frame = +2
Query: 8 TLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGHSKEA 67
T +VA H + KDCW++ING+V D+T FL EHPGGD+VL+ GKD T +F+ VGHS A
Sbjct: 110 TFEEVANHNNKKDCWIIINGKVYDITPFLDEHPGGDEVLVTSTGKDATIDFEDVGHSDSA 289
Query: 68 QNLVLKYQVGILQGATVQEIDVVHKEPNSKQ------EMSAFVIKEDAESKSVALFEFFV 121
++ KY VG + +T+ V H P Q + S FV+K + +F +
Sbjct: 290 IEMMEKYFVGKVDTSTL-PAKVNHSLPQPTQAGGAGNQSSGFVVK---------ILQFLL 439
Query: 122 PLVVATLYF 130
P ++ L F
Sbjct: 440 PFLILGLAF 466
>TC226039 similar to UP|O48618 (O48618) Cytochome b5 (Fragment), partial
(62%)
Length = 775
Score = 93.6 bits (231), Expect = 3e-20
Identities = 45/130 (34%), Positives = 72/130 (54%)
Frame = +3
Query: 2 AERRVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAV 61
++ + FT +VA+H KDCW+++ G+V DVT FL +HPGGD+VL+ KD T +F+ +
Sbjct: 90 SKTKTFTFEEVAKHNHRKDCWIIVKGKVYDVTPFLDDHPGGDEVLVTATEKDATTDFEDI 269
Query: 62 GHSKEAQNLVLKYQVGILQGATVQEIDVVHKEPNSKQEMSAFVIKEDAESKSVALFEFFV 121
GHS A ++ KY VG + T+ V + + A V + V + ++ V
Sbjct: 270 GHSDSATEMMEKYFVGEVDTNTLPA--QVTSSSSVRPPTQAPVYNNQSSGFVVKILQYIV 443
Query: 122 PLVVATLYFG 131
PL++ FG
Sbjct: 444 PLLILAFAFG 473
>TC226040 similar to UP|CYB5_BRAOL (P40934) Cytochrome b5, partial (58%)
Length = 742
Score = 91.3 bits (225), Expect = 1e-19
Identities = 46/138 (33%), Positives = 72/138 (51%), Gaps = 8/138 (5%)
Frame = +2
Query: 2 AERRVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAV 61
++ + FT +VA+H KDCW+++ G+V DVT FL +HPGGD+VL+ KD T +F+ +
Sbjct: 71 SKTKTFTFEEVAKHNHRKDCWIIVKGKVYDVTPFLDDHPGGDEVLVTATEKDATTDFEDI 250
Query: 62 GHSKEAQNLVLKYQVGILQGATV--------QEIDVVHKEPNSKQEMSAFVIKEDAESKS 113
GHS A ++ KY VG + T+ P + S FV+K
Sbjct: 251 GHSDSATQMMEKYFVGEVDTNTLPAQVTSNNSVRQPTQAPPAYNNQSSGFVVK------- 409
Query: 114 VALFEFFVPLVVATLYFG 131
+ ++ VPL++ FG
Sbjct: 410 --MLQYIVPLLILAFAFG 457
>BG405226 similar to GP|2647949|emb cytochrome b5 {Olea europaea}, partial
(92%)
Length = 418
Score = 67.8 bits (164), Expect(2) = 3e-17
Identities = 28/43 (65%), Positives = 39/43 (90%)
Frame = +1
Query: 5 RVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLL 47
+VFTL++V+QH + KDCW+VI+G+V +VTKFL +HPGGD+VLL
Sbjct: 28 KVFTLAEVSQHNNAKDCWLVIHGKVYNVTKFLEDHPGGDEVLL 156
Score = 36.2 bits (82), Expect(2) = 3e-17
Identities = 23/85 (27%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
Frame = +2
Query: 49 VAGKDVTKEFDAVGHSKEAQNLVLKYQVGILQGATV--QEIDVVHKEPNSKQEMSAFVIK 106
+AGKD T +F+ +GHS A ++ ++ VG + +T+ + K+P+ Q+ + I
Sbjct: 185 LAGKDATNDFEDIGHSTSAVAMMDEFYVGDIDTSTIPSKVKYTPPKQPHYNQDKTPEFI- 361
Query: 107 EDAESKSVALFEFFVPLVVATLYFG 131
+ + +F VPL + L G
Sbjct: 362 -------IRILQFLVPLFILGLAVG 415
>BE330689 weakly similar to GP|2695711|emb cytochome b5 {Olea europaea},
partial (89%)
Length = 364
Score = 83.6 bits (205), Expect = 3e-17
Identities = 33/83 (39%), Positives = 55/83 (65%)
Frame = +2
Query: 2 AERRVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAV 61
++R++ T +VA+H KDCW++I+G+ D+T F+ +HP GD+VLL GKD T +F
Sbjct: 5 SDRKLHTFEEVAKHNQTKDCWLIISGKEYDITPFMEDHPRGDEVLLSTTGKDATNDF*YT 184
Query: 62 GHSKEAQNLVLKYQVGILQGATV 84
GH+ +++ KY +G + +TV
Sbjct: 185GHNDSTRHISKKYYIGDIDASTV 253
>BE059104
Length = 212
Score = 73.9 bits (180), Expect = 2e-14
Identities = 38/49 (77%), Positives = 42/49 (85%)
Frame = +2
Query: 88 DVVHKEPNSKQEMSAFVIKEDAESKSVALFEFFVPLVVATLYFGYRCLT 136
DVV KE N+K EMSAFVIKE A SKS+A +EFFVPL+VA LYFGYRCLT
Sbjct: 5 DVVDKESNTK-EMSAFVIKEGARSKSLAFYEFFVPLLVAGLYFGYRCLT 148
>BE210560 weakly similar to SP|Q42342|CY51_ Cytochrome b5 isoform 1.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (52%)
Length = 370
Score = 71.2 bits (173), Expect = 2e-13
Identities = 28/71 (39%), Positives = 49/71 (68%)
Frame = +2
Query: 1 MAERRVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDA 60
+++R++ T +VA+H +DCW++ +G+V DVT F+++ PGGDD LL G D + +F+
Sbjct: 2 VSDRKLHTFDEVAKHNHIQDCWLIFSGKVHDVTPFIVDLPGGDDSLLSATGNDASIDFEY 181
Query: 61 VGHSKEAQNLV 71
VGH A +++
Sbjct: 182VGHKDSAIDMM 214
>TC205882 UP|Q9SYR0 (Q9SYR0) Nitrate reductase (Fragment), complete
Length = 2934
Score = 70.5 bits (171), Expect = 3e-13
Identities = 30/75 (40%), Positives = 52/75 (69%)
Frame = +1
Query: 5 RVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGHS 64
+ +T+S+V +H + W++++G V D T+FL +HPGG D +L AG D T+EF+A+ HS
Sbjct: 1552 KTYTMSEVRRHNNADSAWIIVHGHVYDCTRFLKDHPGGTDSILINAGTDCTEEFEAI-HS 1728
Query: 65 KEAQNLVLKYQVGIL 79
+A+ ++ Y++G L
Sbjct: 1729 DKAKQMLEDYRIGEL 1773
>BE802874
Length = 456
Score = 70.1 bits (170), Expect = 3e-13
Identities = 29/56 (51%), Positives = 39/56 (68%)
Frame = +2
Query: 8 TLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGH 63
T +VA H S KDCW++ING+V D+T FL EHP G+DVL+ KD + + + VGH
Sbjct: 50 TFEEVANHNSTKDCWIIINGKVFDITPFLDEHPEGNDVLVTSTRKDASIDIEHVGH 217
>TC207476 similar to UP|Q6ZA21 (Q6ZA21) Cytochrome b5-like, partial (61%)
Length = 792
Score = 60.1 bits (144), Expect = 4e-10
Identities = 29/89 (32%), Positives = 50/89 (55%), Gaps = 8/89 (8%)
Frame = +1
Query: 7 FTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGHSKE 66
++ ++V+ H DCW++I +V DVT ++ EHPGGD +L+ AG D T+ F H+
Sbjct: 271 YSKTEVSLHNKRTDCWIIIKNKVYDVTSYVEEHPGGDSILVH-AGDDSTEGFFGPQHATR 447
Query: 67 AQNLVLKYQVG--------ILQGATVQEI 87
+++ + +G +LQ VQE+
Sbjct: 448 VFDMIEDFYIGDLEQ*LSQLLQECIVQEM 534
>BI970294
Length = 552
Score = 58.2 bits (139), Expect = 1e-09
Identities = 28/89 (31%), Positives = 49/89 (54%), Gaps = 8/89 (8%)
Frame = -1
Query: 7 FTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGHSKE 66
++ ++++ H DCW++I +V DVT ++ EHPGGD +L AG D T+ F H+
Sbjct: 420 YSKTEISLHNKRTDCWIIIKNKVYDVTSYVEEHPGGDAILAH-AGDDSTEGFFGPQHATR 244
Query: 67 AQNLVLKYQVG--------ILQGATVQEI 87
+++ + +G +LQ VQE+
Sbjct: 243 VFDMIEDFCIGDLEQ*LSQLLQDCNVQEM 157
>TC207475 similar to UP|Q6ZA21 (Q6ZA21) Cytochrome b5-like, partial (61%)
Length = 462
Score = 57.0 bits (136), Expect = 3e-09
Identities = 24/74 (32%), Positives = 44/74 (59%)
Frame = +2
Query: 7 FTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAVGHSKE 66
++ ++++ H DCW++I +V DVT ++ EHPGGD +L AG D T+ F H+
Sbjct: 212 YSKTEISLHNKRTDCWIIIKNKVYDVTSYVEEHPGGDAILAH-AGDDSTEGFFGPQHATR 388
Query: 67 AQNLVLKYQVGILQ 80
+++ + +G L+
Sbjct: 389 VFDMIEDFCIGDLE 430
>TC206988 similar to GB|AAQ62405.1|34146794|BT010404 At5g09680 {Arabidopsis
thaliana;} , partial (68%)
Length = 1121
Score = 52.4 bits (124), Expect = 7e-08
Identities = 24/78 (30%), Positives = 46/78 (58%)
Frame = +2
Query: 2 AERRVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDAV 61
+ +R+ ++ +V +H++ W V+ GRV +++ ++ HPGG ++L++ GKD T F+
Sbjct: 584 SNKRLISMDEVRKHQTEGQMWTVLKGRVYNISPYMKFHPGGVNMLMKAVGKDCTSLFNKY 763
Query: 62 GHSKEAQNLVLKYQVGIL 79
A+ L+ K VG L
Sbjct: 764 HAWVNAEFLLEKCFVGTL 817
>TC231075 weakly similar to UP|Q8LB96 (Q8LB96) Delta-8 sphingolipid
desaturase, partial (41%)
Length = 806
Score = 48.5 bits (114), Expect = 1e-06
Identities = 21/57 (36%), Positives = 35/57 (60%)
Frame = +2
Query: 4 RRVFTLSQVAQHKSNKDCWVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDA 60
++ T ++ +H D W+ I G+V +V+ + +HPGG+ LL +AG+DVT F A
Sbjct: 251 KKYITSEELKEHNKPGDLWISIQGKVYNVSDWAKDHPGGEVPLLNLAGQDVTDAFVA 421
>TC209257 similar to UP|Q8LB96 (Q8LB96) Delta-8 sphingolipid desaturase,
partial (62%)
Length = 1032
Score = 41.6 bits (96), Expect = 1e-04
Identities = 18/39 (46%), Positives = 27/39 (69%)
Frame = +1
Query: 22 WVVINGRVLDVTKFLIEHPGGDDVLLEVAGKDVTKEFDA 60
W+ I G+V +V+ ++ EHPGGD + +AG+DVT F A
Sbjct: 1 WISIQGKVYNVSDWVKEHPGGDVPISNLAGQDVTDAFIA 117
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.136 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,946,628
Number of Sequences: 63676
Number of extensions: 47830
Number of successful extensions: 250
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 250
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 250
length of query: 143
length of database: 12,639,632
effective HSP length: 88
effective length of query: 55
effective length of database: 7,036,144
effective search space: 386987920
effective search space used: 386987920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0050.12