BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0046a.3
(160 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC219569 275 7e-75
AW569307 252 6e-68
BE352758 133 3e-32
AW733733 weakly similar to GP|21536663|gb plasma membrane associ... 117 2e-27
TC229520 UP|Q39872 (Q39872) PGmPM3, complete 107 2e-24
AW459043 similar to GP|6164957|gb| vacuolar H+-ATP synthase 16kD... 28 1.9
TC210772 similar to UP|O80358 (O80358) AtMMH-1 protein (F6D8.28 ... 28 2.4
TC219854 similar to UP|Q75KB9 (Q75KB9) Expressed protein, partia... 27 3.2
TC214701 similar to UP|Q9M3Z0 (Q9M3Z0) 60S ribosomal protein L6,... 27 4.1
TC206857 homologue to UP|Q940N6 (Q940N6) AT5g21070/T10F18_100, p... 27 5.4
TC219121 similar to UP|Q9M9U3 (Q9M9U3) F6A14.17 protein (At1g187... 26 7.1
TC217714 homologue to UP|Q9FI75 (Q9FI75) Similarity to 5'-nucleo... 26 9.2
TC204643 similar to UP|Q9XGC6 (Q9XGC6) Adenosine kinase (Fragmen... 26 9.2
BE824389 26 9.2
TC207085 similar to UP|Q9SH20 (Q9SH20) F28K19.1, partial (22%) 26 9.2
CA853472 26 9.2
BU084231 26 9.2
>TC219569
Length = 790
Score = 275 bits (703), Expect = 7e-75
Identities = 134/160 (83%), Positives = 147/160 (91%)
Frame = +1
Query: 1 MANEQMKPIATLLLGLNFCMYVIVLGIGGWAMNRAIDQGFIIGPELQLPAHFSPIFFPMG 60
MA +QMKPIATLLLGLNFCMYVIVLGIGGWAMNRAID GF+IGP LPAHFSPI+FPMG
Sbjct: 85 MATDQMKPIATLLLGLNFCMYVIVLGIGGWAMNRAIDHGFVIGPGFDLPAHFSPIYFPMG 264
Query: 61 NASTGFFVTFALIAGVVGAASAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWK 120
NA+TGFFVTFALIAGV G S ISG+NH+RSWT+ESLPSAASVA++AW LT+LAMGFA K
Sbjct: 265 NAATGFFVTFALIAGVAGVGSIISGVNHVRSWTSESLPSAASVASIAWALTVLAMGFACK 444
Query: 121 EVELRIRNARLKTMEAFLIILSATQLFYIAAIHGAAAYRR 160
E++L RNARLKTMEAF+IILSATQLFYIAAIHGAAAYRR
Sbjct: 445 EIQLTGRNARLKTMEAFMIILSATQLFYIAAIHGAAAYRR 564
>AW569307
Length = 410
Score = 252 bits (643), Expect = 6e-68
Identities = 123/131 (93%), Positives = 128/131 (96%)
Frame = +2
Query: 1 MANEQMKPIATLLLGLNFCMYVIVLGIGGWAMNRAIDQGFIIGPELQLPAHFSPIFFPMG 60
MA EQMKPIATLLLGLNFCMY IVLGIGGWAMNRAID GFIIGPEL+LPAHFSP+FFPMG
Sbjct: 17 MAKEQMKPIATLLLGLNFCMYAIVLGIGGWAMNRAIDHGFIIGPELKLPAHFSPLFFPMG 196
Query: 61 NASTGFFVTFALIAGVVGAASAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWK 120
NASTGFFVTFAL+AGVVGAASAISGINHI+SWTAESLPSAASVATMAWTLTLLAMGFAWK
Sbjct: 197 NASTGFFVTFALLAGVVGAASAISGINHIQSWTAESLPSAASVATMAWTLTLLAMGFAWK 376
Query: 121 EVELRIRNARL 131
E+ELRIRNARL
Sbjct: 377 EIELRIRNARL 409
>BE352758
Length = 394
Score = 133 bits (335), Expect = 3e-32
Identities = 63/74 (85%), Positives = 67/74 (90%)
Frame = +1
Query: 1 MANEQMKPIATLLLGLNFCMYVIVLGIGGWAMNRAIDQGFIIGPELQLPAHFSPIFFPMG 60
MAN+QMKPIATLLLGLNFCMYVIVLGIGGWAMNRAID GF+IGP LPAHFSPI+FPMG
Sbjct: 103 MANDQMKPIATLLLGLNFCMYVIVLGIGGWAMNRAIDHGFVIGPGFDLPAHFSPIYFPMG 282
Query: 61 NASTGFFVTFALIA 74
A+TGFF TFAL A
Sbjct: 283 XAATGFFGTFALNA 324
>AW733733 weakly similar to GP|21536663|gb plasma membrane associated
protein-like {Arabidopsis thaliana}, partial (71%)
Length = 435
Score = 117 bits (294), Expect = 2e-27
Identities = 57/118 (48%), Positives = 78/118 (65%)
Frame = +2
Query: 7 KPIATLLLGLNFCMYVIVLGIGGWAMNRAIDQGFIIGPELQLPAHFSPIFFPMGNASTGF 66
K +A++LL LN +Y IVL I WA+N I + L +PA PI+FPMGN +TGF
Sbjct: 68 KSVASILLALNLVLYFIVLVIASWAVNHGIQRSGETASVLSIPARIFPIYFPMGNMTTGF 247
Query: 67 FVTFALIAGVVGAASAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWKEVEL 124
FV +L+AGVVG ++++G+ +I W A +L +AA + W LTLLAMGFA KE+EL
Sbjct: 248 FVILSLVAGVVGFTTSLTGLQNIFQWNAPNLHAAAMSSLTTWALTLLAMGFACKEIEL 421
>TC229520 UP|Q39872 (Q39872) PGmPM3, complete
Length = 1018
Score = 107 bits (268), Expect = 2e-24
Identities = 63/146 (43%), Positives = 88/146 (60%), Gaps = 1/146 (0%)
Frame = +3
Query: 9 IATLLLGLNFCMYVIVLGIGGWAMNRAIDQGFIIGPELQLPAHFSPIFFPMGNASTGFFV 68
+A LL LN MY IVLG W +NR FI G + P F GN +T FF+
Sbjct: 174 VAAPLLFLNLIMYFIVLGFASWCLNR-----FINGQT------YHPSFG--GNGATMFFL 314
Query: 69 TFALIAGVVGAASAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWKEVEL-RIR 127
TF+++A V+G S + G NH+R+W ++SL SA + + +AW +T LA G A K++ L R
Sbjct: 315 TFSILAAVLGIVSKLLGGNHMRTWRSDSLASAGATSMVAWAVTALAFGLACKQIHLGGHR 494
Query: 128 NARLKTMEAFLIILSATQLFYIAAIH 153
RL+ +EAF+IIL+ TQL Y+ IH
Sbjct: 495 GWRLRVVEAFIIILTFTQLLYLILIH 572
>AW459043 similar to GP|6164957|gb| vacuolar H+-ATP synthase 16kDa
proteolipid subunit {Dendrobium crumenatum}, partial
(94%)
Length = 469
Score = 28.1 bits (61), Expect = 1.9
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 5/33 (15%)
Frame = +3
Query: 87 NHIRSWTAESLPSAASVAT-----MAWTLTLLA 114
N I SWTA +AAS+A +AWTL L+A
Sbjct: 249 NPITSWTATLTYAAASLAVLLASPLAWTLALVA 347
>TC210772 similar to UP|O80358 (O80358) AtMMH-1 protein (F6D8.28 protein),
partial (72%)
Length = 986
Score = 27.7 bits (60), Expect = 2.4
Identities = 25/94 (26%), Positives = 38/94 (39%), Gaps = 11/94 (11%)
Frame = -3
Query: 49 PAHFSPIFFPMGNASTGFF--------VTFALIAGVVGAASAISGINHIRSWTAESLPSA 100
PA IFFP A GFF V + L++ A +++ + H+ + SL
Sbjct: 792 PAVMKSIFFPSTKALPGFFSRE*NIQLVGY*LLSAPTSIAFSMTSLMHLLKFEQLSLDRL 613
Query: 101 ASVA---TMAWTLTLLAMGFAWKEVELRIRNARL 131
+ +AW T A F E++L N L
Sbjct: 612 EATCRG*ILAWYNTSSATQFPIPEIKL*SSNKAL 511
>TC219854 similar to UP|Q75KB9 (Q75KB9) Expressed protein, partial (26%)
Length = 987
Score = 27.3 bits (59), Expect = 3.2
Identities = 22/69 (31%), Positives = 33/69 (46%)
Frame = +1
Query: 67 FVTFALIAGVVGAASAISGINHIRSWTAESLPSAASVATMAWTLTLLAMGFAWKEVELRI 126
FVT A A ++G A G ++ A+ L AAS A TL ++ M W V R+
Sbjct: 316 FVTGASFALLLGLPVAWFGSAGAQAEPAKRLLCAASSGLFAVTLAVVRMYLGWAYVGNRL 495
Query: 127 RNARLKTME 135
+A ++ E
Sbjct: 496 LSATVEYEE 522
>TC214701 similar to UP|Q9M3Z0 (Q9M3Z0) 60S ribosomal protein L6, partial
(96%)
Length = 931
Score = 26.9 bits (58), Expect = 4.1
Identities = 12/36 (33%), Positives = 22/36 (60%)
Frame = -2
Query: 56 FFPMGNASTGFFVTFALIAGVVGAASAISGINHIRS 91
+FP+ ++GF +T +I GV+ A+S + + RS
Sbjct: 120 YFPIPLINSGFLLTLRVIFGVIAASSCYTTNPNFRS 13
>TC206857 homologue to UP|Q940N6 (Q940N6) AT5g21070/T10F18_100, partial (92%)
Length = 949
Score = 26.6 bits (57), Expect = 5.4
Identities = 21/69 (30%), Positives = 34/69 (48%), Gaps = 8/69 (11%)
Frame = +2
Query: 4 EQMKPIATLLLGLNFCMYVIV--LGIGGWA------MNRAIDQGFIIGPELQLPAHFSPI 55
E + +A+LLL Y+I+ +G GG + M + G I+ P L A P+
Sbjct: 314 ETVHKVASLLLVFLGGSYIILFLMGKGGHSHSHNQPMEKMAVAGLILVPALSPCATTLPV 493
Query: 56 FFPMGNAST 64
F +GN+S+
Sbjct: 494 FLAVGNSSS 520
>TC219121 similar to UP|Q9M9U3 (Q9M9U3) F6A14.17 protein
(At1g18720/F6A14_17), partial (82%)
Length = 856
Score = 26.2 bits (56), Expect = 7.1
Identities = 20/82 (24%), Positives = 33/82 (39%), Gaps = 9/82 (10%)
Frame = +3
Query: 47 QLPAHFSPIFFPMGNAST------GFFVTFALIAGVVGA---ASAISGINHIRSWTAESL 97
Q+ +F+P +G + GFF T V A +++ W A S
Sbjct: 255 QMILYFTPPLLTLGFLPSVLLLNWGFFSTLFYALFYVALDYKAGSLAAFLTFFCWVASSF 434
Query: 98 PSAASVATMAWTLTLLAMGFAW 119
S++ ++AW + L A F W
Sbjct: 435 VSSSLGFSLAWKVVLAAQLFCW 500
>TC217714 homologue to UP|Q9FI75 (Q9FI75) Similarity to 5'-nucleotidase,
partial (5%)
Length = 643
Score = 25.8 bits (55), Expect = 9.2
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 52 FSPIFFPMGNASTGFFVTFA 71
F PIF P+GN ++ F FA
Sbjct: 492 FMPIFHPVGNKNSSIFSPFA 433
>TC204643 similar to UP|Q9XGC6 (Q9XGC6) Adenosine kinase (Fragment), partial
(39%)
Length = 803
Score = 25.8 bits (55), Expect = 9.2
Identities = 14/37 (37%), Positives = 18/37 (47%)
Frame = -2
Query: 44 PELQLPAHFSPIFFPMGNASTGFFVTFALIAGVVGAA 80
P PA F I F +GN+ TG+ TF G+A
Sbjct: 358 PTKASPAPFVSINFSLGNSITGYNFTFPSSVTQTGSA 248
>BE824389
Length = 619
Score = 25.8 bits (55), Expect = 9.2
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = -3
Query: 16 LNFCMYVIVLGIGGWAMNRAIDQG 39
+N+CMY+ LG+ G M+R +QG
Sbjct: 455 INYCMYLQKLGVDG-TMDRRANQG 387
>TC207085 similar to UP|Q9SH20 (Q9SH20) F28K19.1, partial (22%)
Length = 1626
Score = 25.8 bits (55), Expect = 9.2
Identities = 12/31 (38%), Positives = 16/31 (50%), Gaps = 5/31 (16%)
Frame = +1
Query: 86 INHIRS-----WTAESLPSAASVATMAWTLT 111
+NH + WT + LPSA S + WT T
Sbjct: 715 VNHSKDRMMLLWTVQFLPSAVSESPYLWTQT 807
>CA853472
Length = 395
Score = 25.8 bits (55), Expect = 9.2
Identities = 18/71 (25%), Positives = 31/71 (43%)
Frame = +1
Query: 42 IGPELQLPAHFSPIFFPMGNASTGFFVTFALIAGVVGAASAISGINHIRSWTAESLPSAA 101
+G L L HF PIF + + S+ F ++ +G +A +P A
Sbjct: 16 MGEFLDLVEHFRPIFCSLFDTSSAFRCLPLSLSSSIGNPNA-----------QPFIPLAE 162
Query: 102 SVATMAWTLTL 112
+ T+ W+L+L
Sbjct: 163 TSCTLNWSLSL 195
>BU084231
Length = 422
Score = 25.8 bits (55), Expect = 9.2
Identities = 14/37 (37%), Positives = 18/37 (47%)
Frame = -3
Query: 44 PELQLPAHFSPIFFPMGNASTGFFVTFALIAGVVGAA 80
P PA F I F +GN+ TG+ TF G+A
Sbjct: 330 PTKASPAPFVSINFSLGNSITGYNFTFPSSVTQTGSA 220
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.328 0.138 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,937,349
Number of Sequences: 63676
Number of extensions: 87360
Number of successful extensions: 615
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 612
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 613
length of query: 160
length of database: 12,639,632
effective HSP length: 90
effective length of query: 70
effective length of database: 6,908,792
effective search space: 483615440
effective search space used: 483615440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0046a.3