BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0042.2
(173 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC217306 weakly similar to UP|Q6YWQ2 (Q6YWQ2) Acidic 82 kDa prot... 190 3e-49
TC217307 weakly similar to UP|Q6YWQ2 (Q6YWQ2) Acidic 82 kDa prot... 122 6e-29
BM519716 76 9e-15
BG651891 49 2e-06
CF921796 29 0.97
CF921670 29 0.97
TC219079 similar to UP|Q6K6Z8 (Q6K6Z8) Hydroxyproline-rich glyco... 29 0.97
TC204019 similar to GB|AAP21362.1|30102888|BT006554 At2g21970 {A... 28 1.7
TC224641 similar to UP|FDH_SOLTU (Q07511) Formate dehydrogenase,... 28 2.2
TC217145 similar to UP|Q7Y0S8 (Q7Y0S8) Erg-1, partial (69%) 28 2.8
TC205851 similar to GB|AAG53636.1|12407751|AF291712 initiation f... 27 4.8
AW761472 27 4.8
TC215531 UP|HEM2_SOYBN (P43210) Delta-aminolevulinic acid dehydr... 27 4.8
TC216611 27 4.8
TC208192 27 4.8
TC232745 27 6.3
TC224720 weakly similar to UP|Q9AXU0 (Q9AXU0) Major latex-like p... 27 6.3
TC228856 similar to UP|Q93VB2 (Q93VB2) AT3g62770/F26K9_200, part... 27 6.3
TC218124 26 8.2
BI471409 26 8.2
>TC217306 weakly similar to UP|Q6YWQ2 (Q6YWQ2) Acidic 82 kDa protein-like,
partial (76%)
Length = 1123
Score = 190 bits (483), Expect = 3e-49
Identities = 105/190 (55%), Positives = 122/190 (63%), Gaps = 35/190 (18%)
Frame = +3
Query: 19 LRWSQRRHFCAVLCRDDGSHSTSQAESSTITLWKHNSELVHALFVPPNEPTKLNKLLREQ 78
L W + + L DGSH+ Q E+S+ T+ K +SELV LFVPPN+P KLNKLLR+Q
Sbjct: 324 LSWQPQLPIPSSLKATDGSHTKPQTEASS-TVRKSSSELVDGLFVPPNDPKKLNKLLRKQ 500
Query: 79 VKDTTGRIWFDMPAQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQ----- 133
KDT G+ WF+MPAQTI PELQKD L+LRAA+DPKRHYKKG+SKSK LPKYFQ
Sbjct: 501 AKDTAGKNWFNMPAQTITPELQKDLKLLKLRAALDPKRHYKKGDSKSKTLPKYFQVGTVV 680
Query: 134 ------------------------------ASSGARKVREIEEQNQPAGNEKWKVKGGNS 163
A+ RKVREIEEQN+PAGNEKWK+KG NS
Sbjct: 681 DSPLDFFSGRLTKKERKATLADELLSDQNLAAYRKRKVREIEEQNRPAGNEKWKIKGSNS 860
Query: 164 RKRAKERRNY 173
RKRAKERR Y
Sbjct: 861 RKRAKERRIY 890
>TC217307 weakly similar to UP|Q6YWQ2 (Q6YWQ2) Acidic 82 kDa protein-like,
partial (58%)
Length = 590
Score = 122 bits (307), Expect = 6e-29
Identities = 68/119 (57%), Positives = 74/119 (62%), Gaps = 35/119 (29%)
Frame = +3
Query: 90 MPAQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQ---------------- 133
MPAQTI PELQKD L+LRAA+DPKRHYKKG+SKSK LPKYFQ
Sbjct: 3 MPAQTITPELQKDLKLLKLRAALDPKRHYKKGDSKSKTLPKYFQVGTVVDSPLDFFSGRL 182
Query: 134 -------------------ASSGARKVREIEEQNQPAGNEKWKVKGGNSRKRAKERRNY 173
A+ RKVREIEEQN+PAGNEKWK+KG NSRKRAKERR Y
Sbjct: 183 TKKERKATLADELLSDQNLAAYRKRKVREIEEQNRPAGNEKWKIKGSNSRKRAKERRIY 359
>BM519716
Length = 428
Score = 75.9 bits (185), Expect = 9e-15
Identities = 34/45 (75%), Positives = 38/45 (83%)
Frame = +2
Query: 58 VHALFVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTINPELQKD 102
V LFVPPN+P KLNKLLR+Q KDT G+ WF+MPAQTI PELQKD
Sbjct: 2 VDGLFVPPNDPKKLNKLLRKQAKDTAGKNWFNMPAQTITPELQKD 136
>BG651891
Length = 337
Score = 48.5 bits (114), Expect = 2e-06
Identities = 29/72 (40%), Positives = 42/72 (58%)
Frame = +2
Query: 62 FVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTINPELQKDETSLQLRAAIDPKRHYKKG 121
F P K+N + KDT G+ WF+MPAQTI PE +K E +++RAA+D +KG
Sbjct: 50 FCPSK*SQKINST-EKAAKDTAGKNWFNMPAQTITPEFRKIEV-IKVRAALDQSGTTRKG 223
Query: 122 NSKSKALPKYFQ 133
+ + KYF+
Sbjct: 224 -FQIEDTSKYFR 256
Score = 36.2 bits (82), Expect = 0.008
Identities = 32/86 (37%), Positives = 45/86 (52%), Gaps = 3/86 (3%)
Frame = +3
Query: 47 TITLWKHNSELVHALFVPPNEPTKLNKLLREQVKDTTGRIWF---DMPAQTINPELQKDE 103
+I LWK +S V LFVPPN+P K +LR+Q+K G+I P+ + L+ +
Sbjct: 9 SIALWK-SSXTVDGLFVPPNDPKK*T-VLRKQLKILLGKIGSICQPKPSPLSSERLKLLK 182
Query: 104 TSLQLRAAIDPKRHYKKGNSKSKALP 129
L L A +R +SKSK LP
Sbjct: 183 *GLPLTKAALQER-----DSKSKTLP 245
>CF921796
Length = 495
Score = 29.3 bits (64), Expect = 0.97
Identities = 14/42 (33%), Positives = 21/42 (49%)
Frame = +2
Query: 129 PKYFQASSGARKVREIEEQNQPAGNEKWKVKGGNSRKRAKER 170
PK F G ++ +QN P G + KGG SRK +++
Sbjct: 116 PKKFGGEKGKKRPPFSRKQNPPGGGKTPPKKGGTSRKGGQKK 241
>CF921670
Length = 727
Score = 29.3 bits (64), Expect = 0.97
Identities = 19/61 (31%), Positives = 27/61 (44%)
Frame = +2
Query: 53 HNSELVHALFVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTINPELQKDETSLQLRAAI 112
H VHA PP E LL++ + R WF Q + E+Q +Q R+AI
Sbjct: 86 HKDPYVHAPIYPPEEYV----LLKDLLYCDQSRSWFYTYGQQLEKEMQ*LNDGVQYRSAI 253
Query: 113 D 113
+
Sbjct: 254 N 256
>TC219079 similar to UP|Q6K6Z8 (Q6K6Z8) Hydroxyproline-rich
glycoprotein-like, partial (11%)
Length = 1215
Score = 29.3 bits (64), Expect = 0.97
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = -1
Query: 30 VLCRDDGSHSTSQAESSTITLWKHNSELVHALFVPPNEPTKLNKLLREQVKD 81
++ + D S S + +T +L ++ ++ A PP+ +KLN L E+VKD
Sbjct: 834 IIGKKDQSLDASLSFPATFSLAHQSATILGARESPPSALSKLNSLAEERVKD 679
>TC204019 similar to GB|AAP21362.1|30102888|BT006554 At2g21970 {Arabidopsis
thaliana;} , partial (75%)
Length = 922
Score = 28.5 bits (62), Expect = 1.7
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = +1
Query: 2 RKQRTERIRLRSENPTKLRWSQRRHFCAVLC 32
R R R RLRS NP + R R FC+ C
Sbjct: 118 RGNRPVRFRLRSRNPKRRRRKARTSFCSRGC 210
>TC224641 similar to UP|FDH_SOLTU (Q07511) Formate dehydrogenase,
mitochondrial precursor (NAD-dependent formate
dehydrogenase) (FDH) , partial (32%)
Length = 490
Score = 28.1 bits (61), Expect = 2.2
Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 4/96 (4%)
Frame = +1
Query: 52 KHNSELVHALFVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTINPELQKDET-SLQLRA 110
+HN ELVH P++P++ N L R + R + ++ L T + L A
Sbjct: 1 RHNCELVHPF---PSQPSE*NYLHR*SIMAMMKRA----ASSSVRSLLSSSSTFTRNLHA 159
Query: 111 AIDPKRH---YKKGNSKSKALPKYFQASSGARKVRE 143
+ + K+ + KGN +K P + GA +RE
Sbjct: 160 SGEKKKIVGVFYKGNEYAKLNPNFVGCVEGALGIRE 267
>TC217145 similar to UP|Q7Y0S8 (Q7Y0S8) Erg-1, partial (69%)
Length = 999
Score = 27.7 bits (60), Expect = 2.8
Identities = 18/63 (28%), Positives = 29/63 (45%), Gaps = 8/63 (12%)
Frame = +3
Query: 68 PTKLNKLLREQVKDTTGRIWFDMPAQTINPEL--------QKDETSLQLRAAIDPKRHYK 119
PT L ++ E G I ++ P +T NP Q++ TSL L P+RH +
Sbjct: 207 PTSLRSIVVEDHGQVQGGIHYNRPRET-NPRTNLFPRKLPQRNTTSLSLLTLRPPQRHQR 383
Query: 120 KGN 122
+ +
Sbjct: 384 RSH 392
>TC205851 similar to GB|AAG53636.1|12407751|AF291712 initiation factor 3g
{Arabidopsis thaliana;} , partial (45%)
Length = 641
Score = 26.9 bits (58), Expect = 4.8
Identities = 11/29 (37%), Positives = 15/29 (50%)
Frame = -3
Query: 11 LRSENPTKLRWSQRRHFCAVLCRDDGSHS 39
+RS +P RRH C++ C G HS
Sbjct: 186 IRSSSPLSTCPHTRRHICSLGCTSSGCHS 100
>AW761472
Length = 441
Score = 26.9 bits (58), Expect = 4.8
Identities = 19/66 (28%), Positives = 32/66 (47%), Gaps = 8/66 (12%)
Frame = -3
Query: 17 TKLRWSQRRHFCAVL--------CRDDGSHSTSQAESSTITLWKHNSELVHALFVPPNEP 68
TKL S R FC++ D SH + S+ ++LW NS + A+ + +EP
Sbjct: 349 TKLVNSVRSSFCSLSKPSDPI*GVFSDNSHCSVAIGSAVLSLWLVNSPVGLAIVLRKHEP 170
Query: 69 TKLNKL 74
+ ++L
Sbjct: 169 RQHSRL 152
>TC215531 UP|HEM2_SOYBN (P43210) Delta-aminolevulinic acid dehydratase,
chloroplast precursor (Porphobilinogen synthase)
(ALADH) , complete
Length = 1737
Score = 26.9 bits (58), Expect = 4.8
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = -3
Query: 3 KQRTERIRLRSENPTKLRWSQRRHFCAVLCRDDGSH 38
K R +R RL T ++W R H C+ R G H
Sbjct: 469 KSRPKRRRLPVPTRTPVKWK*RNHRCSNRSRRSGRH 362
>TC216611
Length = 634
Score = 26.9 bits (58), Expect = 4.8
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +1
Query: 1 MRKQRTERIRLRSENPTKLRWSQRRHFCAVLCRDDGS 37
++K R+ R L + WSQRR CA + +GS
Sbjct: 238 LQKYRSHRKHLLAREAEAASWSQRRQLCAGGGKREGS 348
>TC208192
Length = 838
Score = 26.9 bits (58), Expect = 4.8
Identities = 16/39 (41%), Positives = 18/39 (46%)
Frame = -3
Query: 57 LVHALFVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTI 95
LV PP + KL K LR Q G F +P QTI
Sbjct: 557 LVFHFLPPPRKKKKLKKELRLQEIIVVGSFIFKVPQQTI 441
>TC232745
Length = 294
Score = 26.6 bits (57), Expect = 6.3
Identities = 10/31 (32%), Positives = 16/31 (51%)
Frame = -1
Query: 11 LRSENPTKLRWSQRRHFCAVLCRDDGSHSTS 41
+ E+ T W FC+ +CRD+ +TS
Sbjct: 210 ITQESRTITAWGMTSFFCSSICRDNDLQTTS 118
>TC224720 weakly similar to UP|Q9AXU0 (Q9AXU0) Major latex-like protein,
partial (92%)
Length = 873
Score = 26.6 bits (57), Expect = 6.3
Identities = 11/19 (57%), Positives = 16/19 (83%)
Frame = +2
Query: 95 INPELQKDETSLQLRAAID 113
I P+LQK ETSLQ++A+ +
Sbjct: 149 IQPQLQKVETSLQIKASAE 205
>TC228856 similar to UP|Q93VB2 (Q93VB2) AT3g62770/F26K9_200, partial (45%)
Length = 847
Score = 26.6 bits (57), Expect = 6.3
Identities = 14/47 (29%), Positives = 21/47 (43%)
Frame = +1
Query: 88 FDMPAQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQA 134
F + + PE +K ++S AAI P + LPKYF +
Sbjct: 313 FSLKVNSSIPEQEKSQSSSNSDAAITPSSSSRSFIKLKGVLPKYFNS 453
>TC218124
Length = 658
Score = 26.2 bits (56), Expect = 8.2
Identities = 13/36 (36%), Positives = 18/36 (49%)
Frame = -1
Query: 136 SGARKVREIEEQNQPAGNEKWKVKGGNSRKRAKERR 171
SG R+VR + G +W+ +GG R A RR
Sbjct: 313 SGRRRVRTV-------GRRRWRHRGGRRRTEAGRRR 227
>BI471409
Length = 424
Score = 26.2 bits (56), Expect = 8.2
Identities = 16/60 (26%), Positives = 26/60 (42%)
Frame = +3
Query: 36 GSHSTSQAESSTITLWKHNSELVHALFVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTI 95
G ST+ S+ +T ++ + A F PT++NK G+ W PAQ +
Sbjct: 183 GLPSTTATSSADLTSNMYSEKEEKATFASEYSPTRINK----------GQYWIPTPAQIL 332
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.313 0.128 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,177,263
Number of Sequences: 63676
Number of extensions: 112618
Number of successful extensions: 565
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 560
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 563
length of query: 173
length of database: 12,639,632
effective HSP length: 91
effective length of query: 82
effective length of database: 6,845,116
effective search space: 561299512
effective search space used: 561299512
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0042.2