BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0037a.3
(1248 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC213164 85 2e-16
BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase... 60 6e-09
BI321779 53 2e-07
CA851030 55 3e-07
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 48 3e-05
TC206673 46 9e-05
BG405621 42 0.002
TC233646 39 0.014
TC234374 39 0.014
TC223473 39 0.019
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 38 0.032
CA936755 37 0.054
AW201165 33 0.79
AI959820 similar to GP|14335118|gb| At1g30200/F12P21_1 {Arabidop... 33 1.0
BI968991 32 1.3
TC227667 weakly similar to UP|Q6Z3T8 (Q6Z3T8) Auxin-regulated pr... 32 1.8
BF009208 similar to PIR|T06785|T06 nonsymbiotic hemoglobin - soy... 32 2.3
TC211438 UP|GLCX_SOYBN (P11827) Beta-conglycinin, alpha' chain p... 32 2.3
TC204168 UP|Q7XXT2 (Q7XXT2) Prepro beta-conglycinin alpha prime ... 32 2.3
BG726231 31 3.0
>TC213164
Length = 446
Score = 85.1 bits (209), Expect = 2e-16
Identities = 42/128 (32%), Positives = 71/128 (54%)
Frame = +3
Query: 286 EADEIKAAVFSMRALKAPGPDGLNTLFFQSQWDTIGSSVVQFIQDCIQRPEKIREVNDTL 345
E E++A ++ K+PGPDGLN F + WD + + +++F+ + ++ N
Sbjct: 33 EKKEVRATMWDRGNAKSPGPDGLNFKFIKEFWDILKTDLLRFLDEFYANGVFLKRSNAFF 212
Query: 346 IVLIPKIDRPNLLSQYRLIGICNVIYKTLTKALANRLKGVLGDLISPNQCSFIPGRQSSD 405
+ LIPK+ P L++Y+ I + IYK + K L+ R K VL +I Q F+ GR +
Sbjct: 213 LALIPKVHDP*SLNEYKPISLIGCIYKIVAKLLSGRHKKVLPTIIDERQTVFMEGRHTLH 392
Query: 406 NIIIAQEV 413
N++IA E+
Sbjct: 393 NVVIANEI 416
>BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (15%)
Length = 376
Score = 60.1 bits (144), Expect = 6e-09
Identities = 28/87 (32%), Positives = 49/87 (56%)
Frame = -2
Query: 290 IKAAVFSMRALKAPGPDGLNTLFFQSQWDTIGSSVVQFIQDCIQRPEKIREVNDTLIVLI 349
IK+AV+ ++K+PGPDGLN F + W+ + +++F+ + + N + I LI
Sbjct: 375 IKSAVWQCGSVKSPGPDGLNFKFIKHFWERLKPDIIRFLNEFHANGIFPKGGNASFIALI 196
Query: 350 PKIDRPNLLSQYRLIGICNVIYKTLTK 376
PK+ P L+ +R I + +YK + K
Sbjct: 195 PKVKHPQALNDFRPISLIGCVYKIVAK 115
>BI321779
Length = 421
Score = 52.8 bits (125), Expect(2) = 2e-07
Identities = 29/91 (31%), Positives = 45/91 (48%)
Frame = +2
Query: 286 EADEIKAAVFSMRALKAPGPDGLNTLFFQSQWDTIGSSVVQFIQDCIQRPEKIREVNDTL 345
E +EIK + + K+PGPDG + L + W+ I V+ F ++ + R N +
Sbjct: 98 EVEEIKKVI*DCESSKSPGPDGYSLLLIKIFWEVIKEEVINFFKEFHKFDFLPRGTNRSF 277
Query: 346 IVLIPKIDRPNLLSQYRLIGICNVIYKTLTK 376
I LI K D P + QY I + +YK + K
Sbjct: 278 IALIAKCDNPLDIGQYCPISLVGCLYKIV*K 370
Score = 21.9 bits (45), Expect(2) = 2e-07
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +3
Query: 369 VIYKTLTKALANRLKGVLGDLI 390
V K K +ANR+ VLG LI
Sbjct: 348 VFIKLYKKIIANRMSIVLGKLI 413
>CA851030
Length = 358
Score = 54.7 bits (130), Expect = 3e-07
Identities = 25/65 (38%), Positives = 34/65 (51%)
Frame = +1
Query: 187 LIQEELLWRQKSRVAWLNHGDKNTRFFHTSTMVRRKRNKIEALTDEHGVSVTDPITLRSM 246
L QEE W+Q+SR W DKNT FFH T+ R+ N I + D+ G P + +
Sbjct: 163 LKQEEEYWQQRSRAIWFKERDKNTEFFHRKTLQRKASNSITKIQDDEGNVCFKPDQIEDI 342
Query: 247 AIDFF 251
I F+
Sbjct: 343 LIKFY 357
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 47.8 bits (112), Expect = 3e-05
Identities = 30/90 (33%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
Frame = +1
Query: 408 IIAQEVFHSMRYLKRKKGWIAIKIDLEKAYDRLEWSFLKDTLLEVGLDRNFCDLILDCVS 467
+IA EV + + K + K+D EKAYD + W+FL + + + I +CV
Sbjct: 13 LIANEVIDEAK--RSNKSCLVFKVDYEKAYDSVSWNFLMYMMWRMDFSPKWIKWIEECVK 186
Query: 468 LSSFQVLFNGSKT-EFPSFQGHPPR*PIIP 496
+S VL NGS T EF +G P+ P
Sbjct: 187 SASISVLVNGSPTAEFLPQRGLRQGDPLTP 276
>TC206673
Length = 742
Score = 46.2 bits (108), Expect = 9e-05
Identities = 26/69 (37%), Positives = 38/69 (54%)
Frame = +3
Query: 161 NSRLRMQHIPYLEKLQKRLWKEYQTTLIQEELLWRQKSRVAWLNHGDKNTRFFHTSTMVR 220
N L + I ++LQ++LW + + E LL RQKSR WL GD N+ +FHT R
Sbjct: 69 NRSLSVDEIKAKKELQQQLW---EASTAYESLL-RQKSRDKWLKEGDNNSAYFHTVINFR 236
Query: 221 RKRNKIEAL 229
R N ++ +
Sbjct: 237 RHYNGLQGI 263
>BG405621
Length = 402
Score = 41.6 bits (96), Expect = 0.002
Identities = 14/29 (48%), Positives = 23/29 (79%)
Frame = +2
Query: 187 LIQEELLWRQKSRVAWLNHGDKNTRFFHT 215
+ QEE W+Q+S++ WL +GD N++FFH+
Sbjct: 314 IAQEESYWQQRSKIFWLKYGDLNSKFFHS 400
>TC233646
Length = 978
Score = 38.9 bits (89), Expect = 0.014
Identities = 23/57 (40%), Positives = 32/57 (55%), Gaps = 1/57 (1%)
Frame = -3
Query: 346 IVLIPKIDRPNLLSQYRLIGICNVIYKTLTKAL-ANRLKGVLGDLISPNQCSFIPGR 401
+VLIPKID +L Y I +CNV + L+++ K I+ NQCSFIP +
Sbjct: 826 LVLIPKIDNLEILK*Y*SISLCNVTTRLLSRSS*PTSSKSFPS*PIARNQCSFIPSK 656
>TC234374
Length = 787
Score = 38.9 bits (89), Expect = 0.014
Identities = 38/140 (27%), Positives = 63/140 (44%), Gaps = 11/140 (7%)
Frame = +2
Query: 181 KEYQTTLIQEELLWRQKSRVAWLNHGDKNTRFF---HTSTM-VRRKRNKIEAL------- 229
KE QT L +E LWR+KSR+ G++NT FF H+ + +R + ++ +
Sbjct: 248 KELQTVLRFQECLWREKSRI*GHAQGEQNTSFFIK*HSIILYLRVYLSYVKGIDY*SLKR 427
Query: 230 TDEHGVSVTDPITLRSMAIDFFQHLYTSPGTDVLYHVRNAFPTLPRDTLLEISNPLEADE 289
+ + ++ PI +R+ + L +Y + + D L SN +E
Sbjct: 428 SSKQRCGISFPIFMRAATLVSLTAL-------*IYRTIPSLVSGEDDAFL--SNIPLNEE 580
Query: 290 IKAAVFSMRALKAPGPDGLN 309
+K+ VF M PG DG N
Sbjct: 581 VKSLVFDMNGEGVPGTDGCN 640
>TC223473
Length = 654
Score = 38.5 bits (88), Expect = 0.019
Identities = 22/65 (33%), Positives = 29/65 (43%)
Frame = +1
Query: 175 LQKRLWKEYQTTLIQEELLWRQKSRVAWLNHGDKNTRFFHTSTMVRRKRNKIEALTDEHG 234
LQ LW+E TL QEE LW K W+ G+ RR+ N I + + G
Sbjct: 298 LQAELWEELHNTLFQEEFLWF*KFHCRWMKFGN------------RRQLNFIHKIQNSSG 441
Query: 235 VSVTD 239
+TD
Sbjct: 442 EWITD 456
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 37.7 bits (86), Expect = 0.032
Identities = 21/62 (33%), Positives = 31/62 (49%)
Frame = -3
Query: 425 GWIAIKIDLEKAYDRLEWSFLKDTLLEVGLDRNFCDLILDCVSLSSFQVLFNGSKTEFPS 484
G +A+KID+ KA+D L+ FL L + G FC+ I + + + NG F S
Sbjct: 771 GNLALKIDIVKAFDTLDRDFLISVLNKFGYSHTFCNWIRVILLSAKLSISVNGEPVGFFS 592
Query: 485 FQ 486
Q
Sbjct: 591 CQ 586
>CA936755
Length = 424
Score = 37.0 bits (84), Expect = 0.054
Identities = 15/36 (41%), Positives = 26/36 (71%)
Frame = -2
Query: 96 LGNDQWLRSFPEASIFHLPQLKSDHKPLLLQLSPID 131
L ++QWL S+P++S + LP+ SDH P+++Q +D
Sbjct: 291 LVSEQWLLSWPDSSQYTLPRDFSDHCPIIMQTKKVD 184
>AW201165
Length = 413
Score = 33.1 bits (74), Expect = 0.79
Identities = 24/82 (29%), Positives = 41/82 (49%)
Frame = -1
Query: 359 SQYRLIGICNVIYKTLTKALANRLKGVLGDLISPNQCSFIPGRQSSDNIIIAQEVFHSMR 418
+ YR I + + K + + RL+ ++ NQ F+PGR + + I + + V +
Sbjct: 248 ANYRGIKLMSHTMKLWERVIERRLRKETQ--VTENQFGFMPGRSTMEAIYLLRRVME--Q 81
Query: 419 YLKRKKGWIAIKIDLEKAYDRL 440
Y ++ I IDLEKA DR+
Sbjct: 80 YRMAQQDLHLIFIDLEKASDRV 15
>AI959820 similar to GP|14335118|gb| At1g30200/F12P21_1 {Arabidopsis
thaliana}, partial (19%)
Length = 342
Score = 32.7 bits (73), Expect = 1.0
Identities = 31/105 (29%), Positives = 47/105 (44%)
Frame = +3
Query: 121 KPLLLQLSPIDIDHSQRPNRQVFGEIGRRKRHLMRRLEGINSRLRMQHIPYLEKLQKRLW 180
+PLL +L+P+ + R+ RR RHL RRL + RLR P LE RL
Sbjct: 21 RPLLRRLAPLPLPRPAGRKRRR----PRRLRHLRRRLLFLRRRLRQVPRPLLEPPPPRL- 185
Query: 181 KEYQTTLIQEELLWRQKSRVAWLNHGDKNTRFFHTSTMVRRKRNK 225
+ + T + R ++ + L H F H + R+RN+
Sbjct: 186 RRNRETHPNPRSVPRPQTPLLLLRH----VPFLHHLLLPPRRRNR 308
>BI968991
Length = 750
Score = 32.3 bits (72), Expect = 1.3
Identities = 12/31 (38%), Positives = 19/31 (60%)
Frame = +1
Query: 189 QEELLWRQKSRVAWLNHGDKNTRFFHTSTMV 219
+ ++LW +KS W+ GD T +FH T+V
Sbjct: 13 KNDILWFRKS*AKWIKDGDHETGYFHGLTLV 105
>TC227667 weakly similar to UP|Q6Z3T8 (Q6Z3T8) Auxin-regulated protein-like,
partial (33%)
Length = 1445
Score = 32.0 bits (71), Expect = 1.8
Identities = 14/29 (48%), Positives = 18/29 (61%), Gaps = 3/29 (10%)
Frame = +3
Query: 33 WVVMGDFNTYL---NAPDKWGGDPPNLLA 58
W + GDF+TYL P WGG+P L+A
Sbjct: 774 WFIEGDFDTYLQRIQQPYVWGGEPELLMA 860
>BF009208 similar to PIR|T06785|T06 nonsymbiotic hemoglobin - soybean, complete
Length = 481
Score = 31.6 bits (70), Expect = 2.3
Identities = 13/40 (32%), Positives = 20/40 (49%)
Frame = -1
Query: 1035 GFVEVSRNGADSIALSFAPCSIKLTRPSAWSL*LQRWQYC 1074
G+ + NG D + + F+PCS+ RP W+ Q C
Sbjct: 475 GWFHLRFNGMDQLIIRFSPCSLHTQRPHYWTSLFYGIQQC 356
>TC211438 UP|GLCX_SOYBN (P11827) Beta-conglycinin, alpha' chain precursor,
complete
Length = 1959
Score = 31.6 bits (70), Expect = 2.3
Identities = 18/51 (35%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
Frame = +2
Query: 186 TLIQEELLWRQKS---RVAWLNHGDKNTRFFHTSTMVRRKRNKIEALTDEH 233
TL+ L WR+K +V + +H D NTR + S VRR++ K+ + H
Sbjct: 200 TLVATSLRWRKKKNAKKVKFHDH-DHNTRRGNVSNTVRRRKTKVSSHVHSH 349
>TC204168 UP|Q7XXT2 (Q7XXT2) Prepro beta-conglycinin alpha prime subunit,
complete
Length = 2055
Score = 31.6 bits (70), Expect = 2.3
Identities = 18/51 (35%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
Frame = +3
Query: 186 TLIQEELLWRQKS---RVAWLNHGDKNTRFFHTSTMVRRKRNKIEALTDEH 233
TL+ L WR+K +V + +H D NTR + S VRR++ K+ + H
Sbjct: 222 TLVATSLRWRKKKNAKKVKFHDH-DHNTRRGNVSNTVRRRKTKVSSHVHSH 371
>BG726231
Length = 415
Score = 31.2 bits (69), Expect = 3.0
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = -1
Query: 92 LDWALGNDQWLRSFPEASIFHLPQLKSDHKPLLLQLSPIDI 132
L W + L + P ++ HL LK H LL+QLSP+ +
Sbjct: 208 LTWKVHVSFCL*TLPSRTMLHLFHLKPPHLKLLMQLSPVSV 86
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.343 0.152 0.539
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,618,853
Number of Sequences: 63676
Number of extensions: 1036554
Number of successful extensions: 6547
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 6437
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6543
length of query: 1248
length of database: 12,639,632
effective HSP length: 108
effective length of query: 1140
effective length of database: 5,762,624
effective search space: 6569391360
effective search space used: 6569391360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0037a.3