BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0035.10
(150 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216325 similar to UP|Q6L3M1 (Q6L3M1) Expressed protein, partia... 30 0.56
TC231989 weakly similar to GB|BAB08855.1|9758354|AB013396 branch... 29 0.73
TC221166 similar to UP|Q9C887 (Q9C887) Myosin-like protein; 9784... 28 1.6
TC206635 similar to UP|Q9SC39 (Q9SC39) Germin-like protein (Frag... 27 2.8
TC229366 similar to UP|AVR3_CHICK (P56733) Avidin-related protei... 27 2.8
TC230777 similar to GB|BAB08884.1|9758397|AB022212 signal recogn... 27 3.6
TC213257 27 3.6
TC204576 similar to UP|Q7XYW2 (Q7XYW2) Seed specific protein Bn1... 27 4.8
BE801609 27 4.8
AI965537 27 4.8
TC216599 similar to UP|Q9S7S1 (Q9S7S1) F3L24.5 protein (MZB10.22... 27 4.8
TC228607 homologue to GB|AAM55304.1|21586462|AY115843 auxin infl... 27 4.8
CF922533 27 4.8
BI320962 27 4.8
CD401767 26 6.2
TC218673 similar to GB|AAL87403.1|19548077|AY081833 At2g20690/F5... 26 6.2
TC228431 homologue to UP|Q9ACM8 (Q9ACM8) SclB protein (Fragment)... 26 6.2
TC206847 similar to GB|AAF76441.1|8569096|F2J10 ESTs gb|AI994059... 26 6.2
BG155136 weakly similar to GP|15294218|gb| AT3g07170/T1B9_17 {Ar... 26 6.2
TC228633 similar to UP|O48700 (O48700) F3I6.27 protein, partial ... 26 6.2
>TC216325 similar to UP|Q6L3M1 (Q6L3M1) Expressed protein, partial (76%)
Length = 1186
Score = 29.6 bits (65), Expect = 0.56
Identities = 33/133 (24%), Positives = 48/133 (35%), Gaps = 7/133 (5%)
Frame = -1
Query: 3 RLVPSLTRMKSGNHRPCCSYITLHMQRKLKKKKKNTTQHNDRDSSEL-------RDQVSI 55
R + S SG CS L L + N Q N S+ + Q+ I
Sbjct: 589 RSIESAVPSSSGIFNLSCSL*IL----LLATGRSNAGQRNSDTKSKFCS*FSASKQQLRI 422
Query: 56 HPSSSSFIITMLLGPSLHQCGWMDGCMPTRNSYDTSGSLSHADTRATSAKATCLLSWFLL 115
P + F+ +L L C +R+S T +KA+ LLSW +L
Sbjct: 421 VPETPFFLNELLESNCLIICFKRPRLGSSRSSLPFRCFEMLTSIEPTCSKASSLLSWEVL 242
Query: 116 HQSLSRSLTYPQC 128
L+ +TY C
Sbjct: 241 QNDLTSFITYKPC 203
>TC231989 weakly similar to GB|BAB08855.1|9758354|AB013396 branched-chain
amino acid aminotransferase-like protein {Arabidopsis
thaliana;} , partial (12%)
Length = 775
Score = 29.3 bits (64), Expect = 0.73
Identities = 15/41 (36%), Positives = 24/41 (57%)
Frame = -1
Query: 39 TQHNDRDSSELRDQVSIHPSSSSFIITMLLGPSLHQCGWMD 79
TQH ++ SS + Q+ HP + +T+L+ P H C W+D
Sbjct: 493 TQHKEQVSSVCQGQLPYHPKAYP-KVTLLILPPFH-CQWVD 377
>TC221166 similar to UP|Q9C887 (Q9C887) Myosin-like protein; 97843-94399,
partial (5%)
Length = 719
Score = 28.1 bits (61), Expect = 1.6
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3
Query: 72 LHQCGWMDGCMPTRNSYDTS 91
L + W DGC P+R Y TS
Sbjct: 330 LWESSWSDGCWPSRREYGTS 389
>TC206635 similar to UP|Q9SC39 (Q9SC39) Germin-like protein (Fragment),
partial (95%)
Length = 897
Score = 27.3 bits (59), Expect = 2.8
Identities = 17/35 (48%), Positives = 20/35 (56%), Gaps = 2/35 (5%)
Frame = +1
Query: 106 ATCLLSWFLLHQSLSRSLTYPQCK--VQTPKKYQV 138
+TCLL +L HQ LS +T CK V PKK V
Sbjct: 76 STCLLPSWL*HQLLSLPMTLVLCKIFVWQPKKKMV 180
>TC229366 similar to UP|AVR3_CHICK (P56733) Avidin-related protein 3
precursor, partial (13%)
Length = 1728
Score = 27.3 bits (59), Expect = 2.8
Identities = 23/104 (22%), Positives = 45/104 (43%), Gaps = 8/104 (7%)
Frame = +3
Query: 7 SLTRMKSGNHRPCCSYITLHMQRKLK-------KKKKNTTQHNDRDSSELRDQVSIHPSS 59
S++ + N +P S++ +L K ++ N+ +SS + + S SS
Sbjct: 93 SISIARKENQKPNPSFLFFRSNNQLSLSNMANGSNDKGSSTTNNNNSSIIPNPSSASASS 272
Query: 60 SSFIITMLLGPSLHQCGWMDGCMPTRNSYDT-SGSLSHADTRAT 102
SSF+ + H G + + N+Y+ S SHA +++
Sbjct: 273 SSFVPNNPRPTANHTVQLFSGTVESFNNYENGSQDFSHATIKSS 404
>TC230777 similar to GB|BAB08884.1|9758397|AB022212 signal recognition
particle 68kD protein-like {Arabidopsis thaliana;} ,
partial (47%)
Length = 1077
Score = 26.9 bits (58), Expect = 3.6
Identities = 15/26 (57%), Positives = 20/26 (76%)
Frame = -2
Query: 46 SSELRDQVSIHPSSSSFIITMLLGPS 71
S +L D+ S+H SS+SFII +LL PS
Sbjct: 641 SIQLLDRHSLHKSSNSFII-VLLSPS 567
>TC213257
Length = 436
Score = 26.9 bits (58), Expect = 3.6
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +2
Query: 11 MKSGNHRPCCSYITLHMQRKLKKKK 35
MK+GNH CC++ + K+K +K
Sbjct: 359 MKNGNHHRCCAHTHHELPLKMKIEK 433
>TC204576 similar to UP|Q7XYW2 (Q7XYW2) Seed specific protein Bn15D17A,
partial (66%)
Length = 1320
Score = 26.6 bits (57), Expect = 4.8
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 70 PSLHQCGWMDGCMPTRNSYDTSGSLSHADTRATSAKATCLLS 111
PSL C W + M S+D+ +LSH A + ++ +LS
Sbjct: 670 PSLVACEWTEEVMNRSGSHDS--TLSHQSEAANAVESESMLS 789
>BE801609
Length = 424
Score = 26.6 bits (57), Expect = 4.8
Identities = 17/54 (31%), Positives = 26/54 (47%)
Frame = -2
Query: 10 RMKSGNHRPCCSYITLHMQRKLKKKKKNTTQHNDRDSSELRDQVSIHPSSSSFI 63
++K G + P S +K KKKKK + RDS R ++ S S+F+
Sbjct: 411 QLKKGTYSPHFSCSFTQQIQKRKKKKKKKERTTQRDSLS*RSHIT---SMSTFL 259
>AI965537
Length = 248
Score = 26.6 bits (57), Expect = 4.8
Identities = 15/31 (48%), Positives = 16/31 (51%), Gaps = 2/31 (6%)
Frame = +1
Query: 12 KSGNHRPCCSYITLHM--QRKLKKKKKNTTQ 40
K N CC Y L M + K KKKK TTQ
Sbjct: 112 KKSNSYNCCCYFILGMYFRPKXKKKKLATTQ 204
>TC216599 similar to UP|Q9S7S1 (Q9S7S1) F3L24.5 protein (MZB10.22 protein),
partial (39%)
Length = 1341
Score = 26.6 bits (57), Expect = 4.8
Identities = 29/97 (29%), Positives = 41/97 (41%), Gaps = 14/97 (14%)
Frame = +3
Query: 36 KNTTQHNDRDSSELR-DQVSIHPSSSSFIITMLLGP------SLHQCGWMDGCMPTRNSY 88
+N +HN S++LR V P+ +I +L SLH G MD + +
Sbjct: 750 ENYLEHNYHGSNKLRLGSVGTVPAEKVAVIELLFPCVFRAVISLHPAGSMDPDVVAFFAP 929
Query: 89 DTSGSLSHAD-------TRATSAKATCLLSWFLLHQS 118
D SGS HA R + A L +FL +QS
Sbjct: 930 DESGSYVHARGFSVHHVFRHITEYAATALQYFLGNQS 1040
>TC228607 homologue to GB|AAM55304.1|21586462|AY115843 auxin influx carrier
protein {Medicago truncatula;} , partial (35%)
Length = 839
Score = 26.6 bits (57), Expect = 4.8
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -1
Query: 28 QRKLKKKKKNTTQHNDRDSS 47
++K KKKKK TT+HN S+
Sbjct: 698 RKKRKKKKKITTKHNQHTSN 639
>CF922533
Length = 593
Score = 26.6 bits (57), Expect = 4.8
Identities = 17/47 (36%), Positives = 24/47 (50%), Gaps = 1/47 (2%)
Frame = +2
Query: 23 ITLHMQRKLKKKKKNTTQHN-DRDSSELRDQVSIHPSSSSFIITMLL 68
+T+H Q LKKK T H+ D D +I SS+S +I+ L
Sbjct: 131 VTIHTQILLKKKSMKQTSHSQDIDRGHKTSNKTILLSSNSSLISPTL 271
>BI320962
Length = 350
Score = 26.6 bits (57), Expect = 4.8
Identities = 14/37 (37%), Positives = 21/37 (55%)
Frame = -2
Query: 8 LTRMKSGNHRPCCSYITLHMQRKLKKKKKNTTQHNDR 44
L MK+G++ + LH K KKKK+ +HND+
Sbjct: 175 LAYMKNGDY---FIFFRLH*IEKQKKKKREKKEHNDQ 74
>CD401767
Length = 673
Score = 26.2 bits (56), Expect = 6.2
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +1
Query: 57 PSSSSFII-TMLLGPSLHQCGWMDGCMPTRNSY 88
P ++ +++ T L+ S H GW+ MPT NSY
Sbjct: 328 PRTNLYLV*TFLVMESPHS-GWLKSSMPTHNSY 423
>TC218673 similar to GB|AAL87403.1|19548077|AY081833 At2g20690/F5H14.34
{Arabidopsis thaliana;} , partial (72%)
Length = 1118
Score = 26.2 bits (56), Expect = 6.2
Identities = 15/42 (35%), Positives = 22/42 (51%), Gaps = 2/42 (4%)
Frame = +3
Query: 20 CSYITLHMQRKLKKKKK--NTTQHNDRDSSELRDQVSIHPSS 59
CS T +R L +++ NTTQH D+ L D + HP +
Sbjct: 42 CSVATSTCRRHLLHREECSNTTQHCQNDNFTLSDLNTHHPEN 167
>TC228431 homologue to UP|Q9ACM8 (Q9ACM8) SclB protein (Fragment), partial
(8%)
Length = 1459
Score = 26.2 bits (56), Expect = 6.2
Identities = 19/70 (27%), Positives = 28/70 (39%), Gaps = 9/70 (12%)
Frame = +2
Query: 76 GWMDGCMPTRNSYDTSGSLSHADTRATSAKAT---------CLLSWFLLHQSLSRSLTYP 126
GW C+ SYDTS L D +A A+ L + LHQ L R++
Sbjct: 746 GWKITCLAMELSYDTSEILCVDDKLDYAANAS*QWSSWVRGYLFLHYWLHQQLIRNVNIA 925
Query: 127 QCKVQTPKKY 136
+ + K +
Sbjct: 926 EATIHFEKGF 955
>TC206847 similar to GB|AAF76441.1|8569096|F2J10 ESTs gb|AI994059, gb|T43740
come from this gene. {Arabidopsis thaliana;} , partial
(46%)
Length = 1571
Score = 26.2 bits (56), Expect = 6.2
Identities = 26/104 (25%), Positives = 40/104 (38%), Gaps = 8/104 (7%)
Frame = +1
Query: 55 IHPSSSSFIITMLL--------GPSLHQCGWMDGCMPTRNSYDTSGSLSHADTRATSAKA 106
+HPSSS F +LL P + + P +S+ S S A S K
Sbjct: 88 VHPSSSPFFCKILLQGLDSNSTSPRVSSVPLISDSDPHPHSFAASFDFSKAQILKASKKP 267
Query: 107 TCLLSWFLLHQSLSRSLTYPQCKVQTPKKYQVVAVGLEMGSAPS 150
L+ S+ + T P C + K V++ L++ A S
Sbjct: 268 -------LMKISVYKGPTTPSCVFTSAKLLGKVSIPLDLTLAES 378
>BG155136 weakly similar to GP|15294218|gb| AT3g07170/T1B9_17 {Arabidopsis
thaliana}, partial (12%)
Length = 486
Score = 26.2 bits (56), Expect = 6.2
Identities = 13/50 (26%), Positives = 27/50 (54%)
Frame = +3
Query: 24 TLHMQRKLKKKKKNTTQHNDRDSSELRDQVSIHPSSSSFIITMLLGPSLH 73
+LH +R L+++ T H+ ++ + +V+ PSS S + P++H
Sbjct: 225 SLHGERDLRERLSGTMTHHPKNYDPPKPKVAAKPSSKSIHVET---PAVH 365
>TC228633 similar to UP|O48700 (O48700) F3I6.27 protein, partial (7%)
Length = 694
Score = 26.2 bits (56), Expect = 6.2
Identities = 16/45 (35%), Positives = 23/45 (50%)
Frame = -1
Query: 12 KSGNHRPCCSYITLHMQRKLKKKKKNTTQHNDRDSSELRDQVSIH 56
KS N P + TL + + KKKK NTT D + +++ IH
Sbjct: 547 KSKN*SPE*NINTLEHEPEKKKKKNNTTWKIDITKRKAKEENKIH 413
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.128 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,584,227
Number of Sequences: 63676
Number of extensions: 129436
Number of successful extensions: 1001
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 992
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 998
length of query: 150
length of database: 12,639,632
effective HSP length: 89
effective length of query: 61
effective length of database: 6,972,468
effective search space: 425320548
effective search space used: 425320548
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0035.10