Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0031.15
         (168 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC227568 similar to UP|Q8VYI3 (Q8VYI3) At1g76150/T23E18_38, part...    33  0.049
BE658430 similar to SP|Q01373|FOX2 Peroxisomal hydratase-dehydro...    32  0.14
TC228523 similar to UP|Q6WDV9 (Q6WDV9) Chitinase class III-1  , ...    30  0.71
AW132301                                                               29  0.93
NP005145 phosphatidylinositol 3-kinase                                 28  1.6
TC228522 weakly similar to UP|Q6WDV9 (Q6WDV9) Chitinase class II...    28  1.6
TC232682 similar to UP|Q7XAS3 (Q7XAS3) Beta-D-glucosidase, parti...    28  2.7
TC209975 weakly similar to UP|Q7T9Y7 (Q7T9Y7) Odv-e66, partial (9%)    27  3.5
TC207276 UP|P3K1_SOYBN (P42347) Phosphatidylinositol 3-kinase, r...    27  3.5
BU549246                                                               27  4.6
TC209322 similar to UP|Q6NLC1 (Q6NLC1) At1g54350, partial (17%)        27  4.6
TC213168 similar to UP|Q97YQ7 (Q97YQ7) ABC transporter, ATP bind...    26  7.9
TC220003 similar to UP|Q6Y2W9 (Q6Y2W9) Atypical receptor-like ki...    26  7.9

>TC227568 similar to UP|Q8VYI3 (Q8VYI3) At1g76150/T23E18_38, partial (73%)
          Length = 1155

 Score = 33.5 bits (75), Expect = 0.049
 Identities = 12/29 (41%), Positives = 20/29 (68%)
 Frame = +2

Query: 50  KVTHDLNPLHTDSAAAQNVGFEGPLVHGM 78
           +++ D NPLH+D   A+  GF  P++HG+
Sbjct: 689 RLSGDYNPLHSDPMVAKVAGFSRPILHGL 775


>BE658430 similar to SP|Q01373|FOX2 Peroxisomal
           hydratase-dehydrogenase-epimerase (HDE) (Multifunctional
           beta-oxidation protein) (MFP), partial (19%)
          Length = 659

 Score = 32.0 bits (71), Expect = 0.14
 Identities = 15/38 (39%), Positives = 24/38 (62%)
 Frame = -3

Query: 41  TEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGM 78
           TEE    Y +++ D NPLH D   ++  GF+ P++HG+
Sbjct: 384 TEEQAAIY-RLSGDRNPLHIDPEFSKVGGFKEPILHGL 274


>TC228523 similar to UP|Q6WDV9 (Q6WDV9) Chitinase class III-1  , partial
           (35%)
          Length = 688

 Score = 29.6 bits (65), Expect = 0.71
 Identities = 16/52 (30%), Positives = 25/52 (47%), Gaps = 6/52 (11%)
 Frame = +2

Query: 42  EEDVLHYSKVTHDLNPLHTDSA------AAQNVGFEGPLVHGMLVASLFPHI 87
           E   LHY+ + + L+  +  S+      AA    F+  L+HG L   LF H+
Sbjct: 86  ESGELHYAALAYKLHDHYAGSSKKFYLTAAPQCPFQNNLLHGALTTGLFDHV 241


>AW132301 
          Length = 421

 Score = 29.3 bits (64), Expect = 0.93
 Identities = 17/54 (31%), Positives = 25/54 (45%), Gaps = 2/54 (3%)
 Frame = -1

Query: 46  LHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLF--PHIISSHFPGAVY 97
           +H  ++    NPLHT S    N+GF        ++   F  PH++S    G VY
Sbjct: 370 IHKKQLLQQRNPLHTFSGRKANLGF*AVSQLHQVLHHCFQKPHLVSDPAHGIVY 209


>NP005145 phosphatidylinositol 3-kinase
          Length = 2439

 Score = 28.5 bits (62), Expect = 1.6
 Identities = 11/26 (42%), Positives = 18/26 (68%)
 Frame = -2

Query: 84  FPHIISSHFPGAVYVSQSLNFKFPVY 109
           FP ++  + P A + +QSL+F FPV+
Sbjct: 458 FPGVLVGNVPSASFPAQSLSFCFPVF 381


>TC228522 weakly similar to UP|Q6WDV9 (Q6WDV9) Chitinase class III-1  ,
           partial (57%)
          Length = 725

 Score = 28.5 bits (62), Expect = 1.6
 Identities = 15/52 (28%), Positives = 24/52 (45%), Gaps = 6/52 (11%)
 Frame = +2

Query: 42  EEDVLHYSKVTHDLNPLHTDS------AAAQNVGFEGPLVHGMLVASLFPHI 87
           E   LHY+ + + L+  +  S       A+    F+  L+HG L   LF H+
Sbjct: 173 ESGELHYAALAYRLHDHYAGSKKKFYLTASPQCSFQNNLLHGALTTGLFDHV 328


>TC232682 similar to UP|Q7XAS3 (Q7XAS3) Beta-D-glucosidase, partial (5%)
          Length = 526

 Score = 27.7 bits (60), Expect = 2.7
 Identities = 13/33 (39%), Positives = 16/33 (48%)
 Frame = +2

Query: 59 HTDSAAAQNVGFEGPLVHGMLVASLFPHIISSH 91
          H  S A   + F    VH  +  SLFPHI+  H
Sbjct: 41 HIASTALGTLAFFRAFVHNFMNPSLFPHILLYH 139


>TC209975 weakly similar to UP|Q7T9Y7 (Q7T9Y7) Odv-e66, partial (9%)
          Length = 537

 Score = 27.3 bits (59), Expect = 3.5
 Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
 Frame = +3

Query: 49  SKVTHDL-NPLHTDSAAAQNVGFEGPLVHGMLVASLFPHIISSHFPGAVYVSQS 101
           SK +H   NPL   SA+  +   + P+ +  +  +L PH   SHFP + Y + S
Sbjct: 111 SKCSHSSKNPLTFFSASFHSYTSKNPISN--ISTTLHPHTSFSHFPTSFYCNTS 266


>TC207276 UP|P3K1_SOYBN (P42347) Phosphatidylinositol 3-kinase, root isoform 
           (PI3-kinase) (PtdIns-3-kinase) (PI3K) (SPI3K-5) ,
           complete
          Length = 2974

 Score = 27.3 bits (59), Expect = 3.5
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = -3

Query: 84  FPHIISSHFPGAVYVSQSLNFKFPV 108
           FP ++  + P A + +QSL+F FPV
Sbjct: 512 FPGVLVGNVPSASFPAQSLSFCFPV 438


>BU549246 
          Length = 639

 Score = 26.9 bits (58), Expect = 4.6
 Identities = 27/96 (28%), Positives = 39/96 (40%), Gaps = 15/96 (15%)
 Frame = -3

Query: 22  AATPQVLKPGDVLRKARAFTE------------EDVLHYSKVTHDLNPLHTDSAAAQNVG 69
           AA P  L P     KA A+TE              V  YS+V +D       SA+ + V 
Sbjct: 511 AAIPASLAPAQQPVKAPAYTEGFGMVRPGGMLDNTVGPYSQVAYD-------SASGRQVY 353

Query: 70  FEGP---LVHGMLVASLFPHIISSHFPGAVYVSQSL 102
           +  P   +VH      +FP + +   PG V + Q +
Sbjct: 352 YTVPGGGVVHAPPYQGVFPPVTADMRPGVVSLGQDV 245


>TC209322 similar to UP|Q6NLC1 (Q6NLC1) At1g54350, partial (17%)
          Length = 883

 Score = 26.9 bits (58), Expect = 4.6
 Identities = 26/109 (23%), Positives = 42/109 (37%), Gaps = 8/109 (7%)
 Frame = -2

Query: 2   LIRSLVSSKLQSL-------SLRCFSSAATPQVLKPGDVLRKARAFTEEDVLHYSKVTHD 54
           L  S + SKL+         S + F S++    +     L K   F ++    +S V  D
Sbjct: 441 LDHSCIESKLKRANKYPRRTSSKTFISSSVVGFIGLSFALSKFGKFVKKGSAFFSLVLSD 262

Query: 55  LNPLHTDSAAAQNVGFEGP-LVHGMLVASLFPHIISSHFPGAVYVSQSL 102
           +    +      N     P  ++G+      P  +  HFP A YVS +L
Sbjct: 261 IGTTSSVQVGYINCSRRVPKTIYGLCGRKKIPDFLLKHFPRASYVSLAL 115


>TC213168 similar to UP|Q97YQ7 (Q97YQ7) ABC transporter, ATP binding
          protein, partial (5%)
          Length = 812

 Score = 26.2 bits (56), Expect = 7.9
 Identities = 10/22 (45%), Positives = 13/22 (58%)
 Frame = -3

Query: 52 THDLNPLHTDSAAAQNVGFEGP 73
          TH L P+H  S+  +  GF GP
Sbjct: 72 THSLQPMHAFSSTNKACGFTGP 7


>TC220003 similar to UP|Q6Y2W9 (Q6Y2W9) Atypical receptor-like kinase MARK,
           partial (36%)
          Length = 1046

 Score = 26.2 bits (56), Expect = 7.9
 Identities = 20/58 (34%), Positives = 22/58 (37%), Gaps = 5/58 (8%)
 Frame = -3

Query: 75  VHGMLVA-----SLFPHIISSHFPGAVYVSQSLNFKFPVYIGDQVIGEVQATNLRANK 127
           VH  L+      SL         P AVY+S   NF    Y G  V G       RANK
Sbjct: 597 VHSFLIQEGMCRSLSCEKFQEQHPKAVYISLLRNFARIGYFGSTVTGNPVGRG*RANK 424


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.320    0.134    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,721,744
Number of Sequences: 63676
Number of extensions: 82914
Number of successful extensions: 447
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 447
length of query: 168
length of database: 12,639,632
effective HSP length: 90
effective length of query: 78
effective length of database: 6,908,792
effective search space: 538885776
effective search space used: 538885776
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)


Lotus: description of TM0031.15