
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0031.15
(168 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227568 similar to UP|Q8VYI3 (Q8VYI3) At1g76150/T23E18_38, part... 33 0.049
BE658430 similar to SP|Q01373|FOX2 Peroxisomal hydratase-dehydro... 32 0.14
TC228523 similar to UP|Q6WDV9 (Q6WDV9) Chitinase class III-1 , ... 30 0.71
AW132301 29 0.93
NP005145 phosphatidylinositol 3-kinase 28 1.6
TC228522 weakly similar to UP|Q6WDV9 (Q6WDV9) Chitinase class II... 28 1.6
TC232682 similar to UP|Q7XAS3 (Q7XAS3) Beta-D-glucosidase, parti... 28 2.7
TC209975 weakly similar to UP|Q7T9Y7 (Q7T9Y7) Odv-e66, partial (9%) 27 3.5
TC207276 UP|P3K1_SOYBN (P42347) Phosphatidylinositol 3-kinase, r... 27 3.5
BU549246 27 4.6
TC209322 similar to UP|Q6NLC1 (Q6NLC1) At1g54350, partial (17%) 27 4.6
TC213168 similar to UP|Q97YQ7 (Q97YQ7) ABC transporter, ATP bind... 26 7.9
TC220003 similar to UP|Q6Y2W9 (Q6Y2W9) Atypical receptor-like ki... 26 7.9
>TC227568 similar to UP|Q8VYI3 (Q8VYI3) At1g76150/T23E18_38, partial (73%)
Length = 1155
Score = 33.5 bits (75), Expect = 0.049
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +2
Query: 50 KVTHDLNPLHTDSAAAQNVGFEGPLVHGM 78
+++ D NPLH+D A+ GF P++HG+
Sbjct: 689 RLSGDYNPLHSDPMVAKVAGFSRPILHGL 775
>BE658430 similar to SP|Q01373|FOX2 Peroxisomal
hydratase-dehydrogenase-epimerase (HDE) (Multifunctional
beta-oxidation protein) (MFP), partial (19%)
Length = 659
Score = 32.0 bits (71), Expect = 0.14
Identities = 15/38 (39%), Positives = 24/38 (62%)
Frame = -3
Query: 41 TEEDVLHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGM 78
TEE Y +++ D NPLH D ++ GF+ P++HG+
Sbjct: 384 TEEQAAIY-RLSGDRNPLHIDPEFSKVGGFKEPILHGL 274
>TC228523 similar to UP|Q6WDV9 (Q6WDV9) Chitinase class III-1 , partial
(35%)
Length = 688
Score = 29.6 bits (65), Expect = 0.71
Identities = 16/52 (30%), Positives = 25/52 (47%), Gaps = 6/52 (11%)
Frame = +2
Query: 42 EEDVLHYSKVTHDLNPLHTDSA------AAQNVGFEGPLVHGMLVASLFPHI 87
E LHY+ + + L+ + S+ AA F+ L+HG L LF H+
Sbjct: 86 ESGELHYAALAYKLHDHYAGSSKKFYLTAAPQCPFQNNLLHGALTTGLFDHV 241
>AW132301
Length = 421
Score = 29.3 bits (64), Expect = 0.93
Identities = 17/54 (31%), Positives = 25/54 (45%), Gaps = 2/54 (3%)
Frame = -1
Query: 46 LHYSKVTHDLNPLHTDSAAAQNVGFEGPLVHGMLVASLF--PHIISSHFPGAVY 97
+H ++ NPLHT S N+GF ++ F PH++S G VY
Sbjct: 370 IHKKQLLQQRNPLHTFSGRKANLGF*AVSQLHQVLHHCFQKPHLVSDPAHGIVY 209
>NP005145 phosphatidylinositol 3-kinase
Length = 2439
Score = 28.5 bits (62), Expect = 1.6
Identities = 11/26 (42%), Positives = 18/26 (68%)
Frame = -2
Query: 84 FPHIISSHFPGAVYVSQSLNFKFPVY 109
FP ++ + P A + +QSL+F FPV+
Sbjct: 458 FPGVLVGNVPSASFPAQSLSFCFPVF 381
>TC228522 weakly similar to UP|Q6WDV9 (Q6WDV9) Chitinase class III-1 ,
partial (57%)
Length = 725
Score = 28.5 bits (62), Expect = 1.6
Identities = 15/52 (28%), Positives = 24/52 (45%), Gaps = 6/52 (11%)
Frame = +2
Query: 42 EEDVLHYSKVTHDLNPLHTDS------AAAQNVGFEGPLVHGMLVASLFPHI 87
E LHY+ + + L+ + S A+ F+ L+HG L LF H+
Sbjct: 173 ESGELHYAALAYRLHDHYAGSKKKFYLTASPQCSFQNNLLHGALTTGLFDHV 328
>TC232682 similar to UP|Q7XAS3 (Q7XAS3) Beta-D-glucosidase, partial (5%)
Length = 526
Score = 27.7 bits (60), Expect = 2.7
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 59 HTDSAAAQNVGFEGPLVHGMLVASLFPHIISSH 91
H S A + F VH + SLFPHI+ H
Sbjct: 41 HIASTALGTLAFFRAFVHNFMNPSLFPHILLYH 139
>TC209975 weakly similar to UP|Q7T9Y7 (Q7T9Y7) Odv-e66, partial (9%)
Length = 537
Score = 27.3 bits (59), Expect = 3.5
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +3
Query: 49 SKVTHDL-NPLHTDSAAAQNVGFEGPLVHGMLVASLFPHIISSHFPGAVYVSQS 101
SK +H NPL SA+ + + P+ + + +L PH SHFP + Y + S
Sbjct: 111 SKCSHSSKNPLTFFSASFHSYTSKNPISN--ISTTLHPHTSFSHFPTSFYCNTS 266
>TC207276 UP|P3K1_SOYBN (P42347) Phosphatidylinositol 3-kinase, root isoform
(PI3-kinase) (PtdIns-3-kinase) (PI3K) (SPI3K-5) ,
complete
Length = 2974
Score = 27.3 bits (59), Expect = 3.5
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -3
Query: 84 FPHIISSHFPGAVYVSQSLNFKFPV 108
FP ++ + P A + +QSL+F FPV
Sbjct: 512 FPGVLVGNVPSASFPAQSLSFCFPV 438
>BU549246
Length = 639
Score = 26.9 bits (58), Expect = 4.6
Identities = 27/96 (28%), Positives = 39/96 (40%), Gaps = 15/96 (15%)
Frame = -3
Query: 22 AATPQVLKPGDVLRKARAFTE------------EDVLHYSKVTHDLNPLHTDSAAAQNVG 69
AA P L P KA A+TE V YS+V +D SA+ + V
Sbjct: 511 AAIPASLAPAQQPVKAPAYTEGFGMVRPGGMLDNTVGPYSQVAYD-------SASGRQVY 353
Query: 70 FEGP---LVHGMLVASLFPHIISSHFPGAVYVSQSL 102
+ P +VH +FP + + PG V + Q +
Sbjct: 352 YTVPGGGVVHAPPYQGVFPPVTADMRPGVVSLGQDV 245
>TC209322 similar to UP|Q6NLC1 (Q6NLC1) At1g54350, partial (17%)
Length = 883
Score = 26.9 bits (58), Expect = 4.6
Identities = 26/109 (23%), Positives = 42/109 (37%), Gaps = 8/109 (7%)
Frame = -2
Query: 2 LIRSLVSSKLQSL-------SLRCFSSAATPQVLKPGDVLRKARAFTEEDVLHYSKVTHD 54
L S + SKL+ S + F S++ + L K F ++ +S V D
Sbjct: 441 LDHSCIESKLKRANKYPRRTSSKTFISSSVVGFIGLSFALSKFGKFVKKGSAFFSLVLSD 262
Query: 55 LNPLHTDSAAAQNVGFEGP-LVHGMLVASLFPHIISSHFPGAVYVSQSL 102
+ + N P ++G+ P + HFP A YVS +L
Sbjct: 261 IGTTSSVQVGYINCSRRVPKTIYGLCGRKKIPDFLLKHFPRASYVSLAL 115
>TC213168 similar to UP|Q97YQ7 (Q97YQ7) ABC transporter, ATP binding
protein, partial (5%)
Length = 812
Score = 26.2 bits (56), Expect = 7.9
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = -3
Query: 52 THDLNPLHTDSAAAQNVGFEGP 73
TH L P+H S+ + GF GP
Sbjct: 72 THSLQPMHAFSSTNKACGFTGP 7
>TC220003 similar to UP|Q6Y2W9 (Q6Y2W9) Atypical receptor-like kinase MARK,
partial (36%)
Length = 1046
Score = 26.2 bits (56), Expect = 7.9
Identities = 20/58 (34%), Positives = 22/58 (37%), Gaps = 5/58 (8%)
Frame = -3
Query: 75 VHGMLVA-----SLFPHIISSHFPGAVYVSQSLNFKFPVYIGDQVIGEVQATNLRANK 127
VH L+ SL P AVY+S NF Y G V G RANK
Sbjct: 597 VHSFLIQEGMCRSLSCEKFQEQHPKAVYISLLRNFARIGYFGSTVTGNPVGRG*RANK 424
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.134 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,721,744
Number of Sequences: 63676
Number of extensions: 82914
Number of successful extensions: 447
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 447
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 447
length of query: 168
length of database: 12,639,632
effective HSP length: 90
effective length of query: 78
effective length of database: 6,908,792
effective search space: 538885776
effective search space used: 538885776
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0031.15