BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0030.2
(82 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC224826 similar to UP|RS21_ORYSA (P35687) 40S ribosomal protein... 159 2e-40
TC224998 similar to UP|RS21_ORYSA (P35687) 40S ribosomal protein... 159 3e-40
TC224971 similar to UP|RS21_ORYSA (P35687) 40S ribosomal protein... 115 9e-35
AW666165 weakly similar to SP|P35687|RS21_O 40S ribosomal protei... 118 5e-28
TC225000 similar to UP|Q7Y199 (Q7Y199) 40S subunit ribosomal pro... 97 1e-21
AW508414 similar to SP|Q9M337|R21B_A 40S ribosomal protein S21-2... 84 1e-17
AW309983 60 2e-10
TC224997 homologue to UP|Q7Y199 (Q7Y199) 40S subunit ribosomal p... 46 3e-06
BI788111 28 1.1
BF595917 27 1.4
BM527600 similar to PIR|T05883|T058 ATP-dependent helicase F6H11... 25 9.1
TC208134 homologue to UP|Q94KA7 (Q94KA7) BZIP transcription fact... 25 9.1
TC208132 homologue to UP|Q94KA7 (Q94KA7) BZIP transcription fact... 25 9.1
>TC224826 similar to UP|RS21_ORYSA (P35687) 40S ribosomal protein S21,
complete
Length = 732
Score = 159 bits (402), Expect = 2e-40
Identities = 74/82 (90%), Positives = 80/82 (97%)
Frame = +3
Query: 1 MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGHLDESGVYNGQFSTYALCGYIRA 60
MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGH+DE+GVYNG FST+ALCG+IRA
Sbjct: 255 MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGHVDENGVYNGHFSTFALCGFIRA 434
Query: 61 QGDADSAIDRLWQKKKAEIKQQ 82
QGDADSA+DRLWQKKK E+KQQ
Sbjct: 435 QGDADSALDRLWQKKKVEVKQQ 500
>TC224998 similar to UP|RS21_ORYSA (P35687) 40S ribosomal protein S21,
complete
Length = 907
Score = 159 bits (401), Expect = 3e-40
Identities = 73/82 (89%), Positives = 80/82 (97%)
Frame = +1
Query: 1 MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGHLDESGVYNGQFSTYALCGYIRA 60
MQNEEGQITELYIPRKCSATNRLITAKDHASVQ+NIGH+DE+GVYNG FST+ALCG+IRA
Sbjct: 133 MQNEEGQITELYIPRKCSATNRLITAKDHASVQVNIGHVDENGVYNGHFSTFALCGFIRA 312
Query: 61 QGDADSAIDRLWQKKKAEIKQQ 82
QGDADSA+DRLWQKKK E+KQQ
Sbjct: 313 QGDADSALDRLWQKKKVEVKQQ 378
>TC224971 similar to UP|RS21_ORYSA (P35687) 40S ribosomal protein S21,
complete
Length = 412
Score = 115 bits (287), Expect(2) = 9e-35
Identities = 53/58 (91%), Positives = 57/58 (97%)
Frame = +2
Query: 1 MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGHLDESGVYNGQFSTYALCGYI 58
MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGH+DE+GVYNG FST+ALCG+I
Sbjct: 119 MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGHVDENGVYNGHFSTFALCGFI 292
Score = 47.0 bits (110), Expect(2) = 9e-35
Identities = 20/23 (86%), Positives = 22/23 (94%)
Frame = +1
Query: 60 AQGDADSAIDRLWQKKKAEIKQQ 82
AQGDADSA+DRLWQKKK E+KQQ
Sbjct: 298 AQGDADSALDRLWQKKKVEVKQQ 366
>AW666165 weakly similar to SP|P35687|RS21_O 40S ribosomal protein S21.
[Rice] {Oryza sativa}, complete
Length = 412
Score = 118 bits (296), Expect = 5e-28
Identities = 53/82 (64%), Positives = 67/82 (81%)
Frame = +2
Query: 1 MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGHLDESGVYNGQFSTYALCGYIRA 60
M NEEG IT+LYIP+KCSATN+LIT KDH SVQ+NI H+DE+G+YNG FST+AL +I
Sbjct: 56 MXNEEGHITKLYIPKKCSATNQLITTKDHTSVQVNIEHVDENGIYNGHFSTFALYSFIHT 235
Query: 61 QGDADSAIDRLWQKKKAEIKQQ 82
QGD ++ +D L QKKK ++KQQ
Sbjct: 236 QGDTNNTLDHL*QKKKVKVKQQ 301
>TC225000 similar to UP|Q7Y199 (Q7Y199) 40S subunit ribosomal protein,
partial (61%)
Length = 658
Score = 97.4 bits (241), Expect = 1e-21
Identities = 42/51 (82%), Positives = 49/51 (95%)
Frame = +2
Query: 32 VQINIGHLDESGVYNGQFSTYALCGYIRAQGDADSAIDRLWQKKKAEIKQQ 82
+QINIGH+DE+GVYNG FST+ALCG+IRAQGDADSA+DRLWQKKK E+KQQ
Sbjct: 242 IQINIGHVDENGVYNGHFSTFALCGFIRAQGDADSALDRLWQKKKVEVKQQ 394
>AW508414 similar to SP|Q9M337|R21B_A 40S ribosomal protein S21-2.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (42%)
Length = 238
Score = 84.3 bits (207), Expect = 1e-17
Identities = 45/79 (56%), Positives = 56/79 (69%), Gaps = 1/79 (1%)
Frame = +2
Query: 2 QNEEGQITELYIPRKCSATNRLITA-KDHASVQINIGHLDESGVYNGQFSTYALCGYIRA 60
QN +GQ TE YIPRKCS NR T+ HA+ QINI H+D++GV G S+ AL RA
Sbjct: 2 QNGDGQGTEHYIPRKCSNANRWCTSIMHHAADQINIAHVDDNGVNIGHTSSDALSRCKRA 181
Query: 61 QGDADSAIDRLWQKKKAEI 79
QGDADSA+DRLW+ K+ E+
Sbjct: 182QGDADSALDRLWKGKRGEL 238
>AW309983
Length = 235
Score = 60.1 bits (144), Expect = 2e-10
Identities = 33/73 (45%), Positives = 45/73 (61%)
Frame = -1
Query: 1 MQNEEGQITELYIPRKCSATNRLITAKDHASVQINIGHLDESGVYNGQFSTYALCGYIRA 60
MQ QI +L+I R S+ + LITA+ VQINIGH E+G + +F+T AL G+IR
Sbjct: 235 MQLHTPQILQLFIHRN*SSVSSLITARPQDMVQINIGHGQENGFFYSRFTTLALFGFIRV 56
Query: 61 QGDADSAIDRLWQ 73
++ SAI R Q
Sbjct: 55 HRNSSSAIGRFGQ 17
>TC224997 homologue to UP|Q7Y199 (Q7Y199) 40S subunit ribosomal protein,
partial (27%)
Length = 992
Score = 46.2 bits (108), Expect = 3e-06
Identities = 21/34 (61%), Positives = 25/34 (72%)
Frame = +1
Query: 49 FSTYALCGYIRAQGDADSAIDRLWQKKKAEIKQQ 82
F + C + QGDADSA+DRLWQKKK E+KQQ
Sbjct: 817 FLAHVFC-VLNFQGDADSALDRLWQKKKVEVKQQ 915
>BI788111
Length = 290
Score = 27.7 bits (60), Expect = 1.1
Identities = 20/56 (35%), Positives = 28/56 (49%)
Frame = +1
Query: 14 PRKCSATNRLITAKDHASVQINIGHLDESGVYNGQFSTYALCGYIRAQGDADSAID 69
P +A N ++++KDH S+ I G SG NG+FS GY +G S D
Sbjct: 85 PAPPAAPNIVMSSKDHDSLFIGGGISFLSGSRNGRFS----YGYSSXKGKRSSMED 240
>BF595917
Length = 419
Score = 27.3 bits (59), Expect = 1.4
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = +2
Query: 40 DESGVYNGQFSTYALCGYIRAQGDADSAIDRLWQKK 75
D+SG Y Q STY L +G+ D A + LW K
Sbjct: 5 DQSGRYTVQ-STYKLMTGEAVEGERDRAFEELWNLK 109
>BM527600 similar to PIR|T05883|T058 ATP-dependent helicase F6H11.20 -
Arabidopsis thaliana, partial (8%)
Length = 422
Score = 24.6 bits (52), Expect = 9.1
Identities = 11/36 (30%), Positives = 18/36 (49%)
Frame = -3
Query: 17 CSATNRLITAKDHASVQINIGHLDESGVYNGQFSTY 52
CS N K ++N+ H+ ES ++NG S +
Sbjct: 396 CSTKNIFSNQKRWNCPRLNLSHVVESHIHNGFISRF 289
>TC208134 homologue to UP|Q94KA7 (Q94KA7) BZIP transcription factor 3,
partial (39%)
Length = 570
Score = 24.6 bits (52), Expect = 9.1
Identities = 11/30 (36%), Positives = 18/30 (59%)
Frame = +3
Query: 53 ALCGYIRAQGDADSAIDRLWQKKKAEIKQQ 82
A+ G + G DSA +LW + + E+K+Q
Sbjct: 339 AVAGGMVTVGSRDSAQSQLWLQDERELKRQ 428
>TC208132 homologue to UP|Q94KA7 (Q94KA7) BZIP transcription factor 3,
partial (33%)
Length = 706
Score = 24.6 bits (52), Expect = 9.1
Identities = 11/30 (36%), Positives = 18/30 (59%)
Frame = +1
Query: 53 ALCGYIRAQGDADSAIDRLWQKKKAEIKQQ 82
A+ G + G DSA +LW + + E+K+Q
Sbjct: 103 AVAGGMVTVGSRDSAQSQLWLQDERELKRQ 192
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.315 0.131 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,173,721
Number of Sequences: 63676
Number of extensions: 27316
Number of successful extensions: 100
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of query: 82
length of database: 12,639,632
effective HSP length: 58
effective length of query: 24
effective length of database: 8,946,424
effective search space: 214714176
effective search space used: 214714176
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0030.2