BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0030.13
(117 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE554918 178 4e-46
TC223288 weakly similar to UP|Q6K621 (Q6K621) Exostosin-like pro... 178 4e-46
TC220306 similar to UP|Q9FFN2 (Q9FFN2) Similarity to limonene cy... 125 3e-30
TC230930 similar to UP|Q94AA9 (Q94AA9) AT5g33290/F19N2_10, parti... 101 6e-23
TC224310 97 1e-21
AW759204 similar to GP|9758523|dbj contains similarity to limone... 84 1e-17
TC203566 similar to UP|Q9FFN2 (Q9FFN2) Similarity to limonene cy... 64 1e-11
TC220548 weakly similar to UP|Q8LQ56 (Q8LQ56) Limonene cyclase-l... 63 2e-11
AW570006 weakly similar to GP|9758008|dbj| contains similarity t... 62 3e-11
BM107757 38 2e-06
TC207994 weakly similar to UP|Q9FZJ1 (Q9FZJ1) F17L21.23, partial... 47 2e-06
BE440603 46 2e-06
TC220363 similar to UP|Q6NMM8 (Q6NMM8) At5g22940, partial (22%) 42 4e-05
AW277542 39 4e-04
TC230561 homologue to UP|Q940Q8 (Q940Q8) AT5g61840/mac9_140, par... 38 6e-04
BI498458 32 0.043
TC209737 similar to UP|Q9LVB4 (Q9LVB4) Gb|AAD21751.1, partial (51%) 31 0.096
TC206958 similar to GB|AAG40731.1|11875626|AF286718 XRN4 {Arabid... 31 0.096
TC209495 28 0.62
TC219697 27 1.1
>BE554918
Length = 425
Score = 178 bits (451), Expect = 4e-46
Identities = 76/117 (64%), Positives = 98/117 (82%)
Frame = +1
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPF 60
M+KRLKVW Y+EGEQPLVHDGP+ YSIEGQF+DE+D SPFKA HP++A+LFL+P+
Sbjct: 22 MMKRLKVWAYKEGEQPLVHDGPVNNKYSIEGQFIDEMDMASMSPFKATHPEQAHLFLLPY 201
Query: 61 SISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
S+S +++YVY P S+ DY RLQRLV DY++++A++YPYWNRS GADHFL+SCHD
Sbjct: 202 SVSKVIRYVYKPRRSRSDYDPDRLQRLVADYINILANRYPYWNRSKGADHFLVSCHD 372
>TC223288 weakly similar to UP|Q6K621 (Q6K621) Exostosin-like protein,
partial (19%)
Length = 834
Score = 178 bits (451), Expect = 4e-46
Identities = 76/117 (64%), Positives = 98/117 (82%)
Frame = +2
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPF 60
M+KRLKVW Y+EGEQPLVHDGP+ YSIEGQF+DE+D SPFKA HP++A+LFL+P+
Sbjct: 434 MMKRLKVWAYKEGEQPLVHDGPVNNKYSIEGQFIDEMDMASMSPFKATHPEQAHLFLLPY 613
Query: 61 SISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
S+S +++YVY P S+ DY RLQRLV DY++++A++YPYWNRS GADHFL+SCHD
Sbjct: 614 SVSKVIRYVYKPRRSRSDYDPDRLQRLVADYINILANRYPYWNRSKGADHFLVSCHD 784
>TC220306 similar to UP|Q9FFN2 (Q9FFN2) Similarity to limonene cyclase,
partial (86%)
Length = 1460
Score = 125 bits (314), Expect = 3e-30
Identities = 56/117 (47%), Positives = 83/117 (70%)
Frame = +2
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPF 60
M K+ KV+VYEEGE P+ H+GP K +YS+EG F+ I+ F+ R P+EA++F +PF
Sbjct: 377 MEKQFKVFVYEEGEPPVFHNGPCKSIYSMEGNFIHAIEM--NDQFRTRDPEEAHVFFLPF 550
Query: 61 SISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
S++ +VQ+VY+ + + G +++ V DYV+V+ +YPYWNRS GADHF L+CHD
Sbjct: 551 SVAMLVQFVYV----RDSHDFGPIKKTVTDYVNVIGGRYPYWNRSLGADHFYLACHD 709
>TC230930 similar to UP|Q94AA9 (Q94AA9) AT5g33290/F19N2_10, partial (66%)
Length = 911
Score = 101 bits (251), Expect = 6e-23
Identities = 51/83 (61%), Positives = 63/83 (75%), Gaps = 1/83 (1%)
Frame = +3
Query: 36 EIDSCRK-SPFKARHPDEANLFLIPFSISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHV 94
E+D+ K S F+AR+P++A++FLIPFSI NIVQYVY L Q +Q LVEDY+ V
Sbjct: 3 EMDNYHKWSHFRARNPNQAHVFLIPFSIVNIVQYVYNRNLRQPGSQ--SIQLLVEDYIRV 176
Query: 95 VADKYPYWNRSNGADHFLLSCHD 117
+A KYPYWNR+ GADHFLLSCHD
Sbjct: 177 IAHKYPYWNRTEGADHFLLSCHD 245
>TC224310
Length = 795
Score = 96.7 bits (239), Expect = 1e-21
Identities = 46/117 (39%), Positives = 76/117 (64%)
Frame = +1
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPF 60
M + LKV+VY EG +P++H GLY+ EG FM ++++ ++ F R P++A+LF +PF
Sbjct: 238 MEQTLKVYVYREGARPIMHSPFFTGLYASEGWFMKQMEANKR--FLTRDPNKAHLFYLPF 411
Query: 61 SISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
S + + +Y+ Q ++ L + + +YV ++A KY +WNR+ GADHFL+ CHD
Sbjct: 412 SSRMLEETLYV----QNSHNHKNLVQYLHNYVEMIAGKYTFWNRTGGADHFLVGCHD 570
>AW759204 similar to GP|9758523|dbj contains similarity to limonene
cyclase~gene_id:K15O15.2 {Arabidopsis thaliana}, partial
(18%)
Length = 427
Score = 84.0 bits (206), Expect = 1e-17
Identities = 42/115 (36%), Positives = 71/115 (61%)
Frame = +1
Query: 1 MVKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPF 60
M ++LKV++Y EG +P+ H ++G+Y+ EG FM ++ ++ F + P +A+LF +PF
Sbjct: 115 MERKLKVFIYREGAKPIFHQPKMRGIYASEGWFMKLMEGNKR--FIVKDPRKAHLFYLPF 288
Query: 61 SISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSC 115
S + + P Q + HL E YV ++A +Y +WNR++GADHFL++C
Sbjct: 289 SSQMLRVTLSNP--KQMEQHL-------EKYVELIAGRYRFWNRTDGADHFLVAC 426
>TC203566 similar to UP|Q9FFN2 (Q9FFN2) Similarity to limonene cyclase,
partial (62%)
Length = 951
Score = 63.5 bits (153), Expect = 1e-11
Identities = 26/39 (66%), Positives = 31/39 (78%)
Frame = +1
Query: 79 YHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
Y G + R V DYV+V+A +YPYWNRS GADHF+LSCHD
Sbjct: 1 YDFGPI*RTVRDYVNVIATRYPYWNRSLGADHFMLSCHD 117
>TC220548 weakly similar to UP|Q8LQ56 (Q8LQ56) Limonene cyclase-like protein,
partial (43%)
Length = 855
Score = 63.2 bits (152), Expect = 2e-11
Identities = 32/92 (34%), Positives = 56/92 (60%)
Frame = +1
Query: 26 LYSIEGQFMDEIDSCRKSPFKARHPDEANLFLIPFSISNIVQYVYMPILSQQDYHLGRLQ 85
LY+ EG FM ++ + F + P +A+LF +PFS + +Y+ + ++ L+
Sbjct: 1 LYASEGWFMKLMEENKH--FVLKDPAKAHLFYMPFSSRMLEHALYV----RNSHNRTNLR 162
Query: 86 RLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
+ ++DY ++ KY Y+NR+ GADHFL++CHD
Sbjct: 163 QFLKDYTDKISAKYRYFNRTGGADHFLVACHD 258
>AW570006 weakly similar to GP|9758008|dbj| contains similarity to limonene
cyclase~gene_id:MED24.9 {Arabidopsis thaliana}, partial
(8%)
Length = 404
Score = 62.4 bits (150), Expect = 3e-11
Identities = 25/69 (36%), Positives = 42/69 (60%)
Frame = +3
Query: 48 RHPDEANLFLIPFSISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNG 107
+ P EA+LF +PFS++ + + +G +Q + DY+H ++ +YPYWN + G
Sbjct: 9 KDPPEADLFFLPFSMARLWH--------DRRVGVGGIQDFIRDYIHNISHRYPYWNNTGG 164
Query: 108 ADHFLLSCH 116
ADHF ++CH
Sbjct: 165 ADHFYVACH 191
>BM107757
Length = 607
Score = 38.1 bits (87), Expect(2) = 2e-06
Identities = 17/33 (51%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = +1
Query: 6 KVWVYEEGEQ-PLVHDGPLKGLYSIEGQFMDEI 37
+ WV + E+ PL HDGP +YS GQF+DEI
Sbjct: 385 RCWVVMKXEKXPLXHDGPANDIYSXXGQFLDEI 483
Score = 28.1 bits (61), Expect(2) = 2e-06
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +2
Query: 53 ANLFLIPFSISNIVQYVYMPI 73
+ +F +PFSI V YVY PI
Sbjct: 533 STIFFLPFSIPMFVHYVYXPI 595
>TC207994 weakly similar to UP|Q9FZJ1 (Q9FZJ1) F17L21.23, partial (12%)
Length = 1377
Score = 46.6 bits (109), Expect = 2e-06
Identities = 18/31 (58%), Positives = 23/31 (74%)
Frame = +3
Query: 87 LVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
L ++YV KYPYWNR+ GADHF ++CHD
Sbjct: 585 LHKNYVESWISKYPYWNRTLGADHFFVTCHD 677
>BE440603
Length = 487
Score = 46.2 bits (108), Expect = 2e-06
Identities = 33/120 (27%), Positives = 57/120 (47%), Gaps = 4/120 (3%)
Frame = +2
Query: 2 VKRLKVWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRK---SPFKARHPDEANLFLI 58
+K+LKV+VY+ P + L + F E+ R S + P +A+ F +
Sbjct: 68 LKKLKVFVYDL--PPKYNTDWLTNERCSKHLFASEVAIHRALLTSEVRTFDPYDADFFFV 241
Query: 59 PFSIS-NIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCHD 117
P +S N P + G + L+ V++++ +YP+WNRS G+DH ++ HD
Sbjct: 242 PVYVSCNFSTVNGFPAI-------GHARSLIASAVNLISSEYPFWNRSRGSDHVFVASHD 400
>TC220363 similar to UP|Q6NMM8 (Q6NMM8) At5g22940, partial (22%)
Length = 775
Score = 42.0 bits (97), Expect = 4e-05
Identities = 32/120 (26%), Positives = 57/120 (46%), Gaps = 5/120 (4%)
Frame = +3
Query: 2 VKRLKVWVYEEGEQP----LVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARHPDEANLFL 57
+K LKV+VY+ ++ L ++ K L++ E + + F P +A+ F
Sbjct: 444 LKNLKVFVYDLPQKYNTDWLSNERCSKHLFASEVAIHRALLTSEVRTFD---PYDADFFF 614
Query: 58 IPFSIS-NIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLSCH 116
+P +S N P + G + L+ V +V+ +YP+WNRS G+DH ++ H
Sbjct: 615 VPVYVSCNFSTVNGFPAI-------GHARSLIASAVSLVSSEYPFWNRSRGSDHVFVASH 773
>AW277542
Length = 434
Score = 38.9 bits (89), Expect = 4e-04
Identities = 20/44 (45%), Positives = 29/44 (65%), Gaps = 5/44 (11%)
Frame = +3
Query: 72 PILSQQDYHLG-----RLQRLVEDYVHVVADKYPYWNRSNGADH 110
P LS Q+ H+G L+ + Y+H+V ++YPYWNRS+G DH
Sbjct: 9 PHLSMQE-HMGLRSSLTLEYYKKAYIHIV-EQYPYWNRSSGRDH 134
>TC230561 homologue to UP|Q940Q8 (Q940Q8) AT5g61840/mac9_140, partial (69%)
Length = 1069
Score = 38.1 bits (87), Expect = 6e-04
Identities = 12/26 (46%), Positives = 19/26 (72%)
Frame = +1
Query: 92 VHVVADKYPYWNRSNGADHFLLSCHD 117
+ +++ +PYWNR+ GADHF + HD
Sbjct: 7 IQLISSNWPYWNRTEGADHFFVVPHD 84
>BI498458
Length = 421
Score = 32.0 bits (71), Expect = 0.043
Identities = 20/67 (29%), Positives = 34/67 (49%), Gaps = 2/67 (2%)
Frame = +2
Query: 50 PDEANLFLIPF--SISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNG 107
P+EA+LF +PF S+S IV V P + ++ + +K YW R++G
Sbjct: 56 PEEADLFFVPFFSSLSLIVNPVRPPGSNSGSEKPVYSDEENQEALVEWLEKQEYWKRNSG 235
Query: 108 ADHFLLS 114
DH +++
Sbjct: 236 RDHVIVA 256
>TC209737 similar to UP|Q9LVB4 (Q9LVB4) Gb|AAD21751.1, partial (51%)
Length = 1120
Score = 30.8 bits (68), Expect = 0.096
Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
Frame = +3
Query: 50 PDEANLFLIPFSISNIV-QYVYMPILSQQ-DYHLGRLQRLVEDYVHVVADKYPYWNRSNG 107
P+ A F IPF V +Y++ +++ D H + + ++D P++ RSNG
Sbjct: 3 PESATAFYIPFYAGLAVGKYLWFNSTAEERDRHCDMMLQWIQDQ--------PFFKRSNG 158
Query: 108 ADHFL 112
DHF+
Sbjct: 159 WDHFI 173
>TC206958 similar to GB|AAG40731.1|11875626|AF286718 XRN4 {Arabidopsis
thaliana;} , partial (6%)
Length = 1605
Score = 30.8 bits (68), Expect = 0.096
Identities = 29/129 (22%), Positives = 53/129 (40%), Gaps = 21/129 (16%)
Frame = +1
Query: 7 VWVYEEGEQPLVHDGPLKGLYSIEGQFMDEIDSCRKSPFKARH-PDEANLFLIPFSISNI 65
+W+YE + P+KGL IE F ++ +P K RH P + L+P I
Sbjct: 190 LWLYERNVLRTIVSSPIKGLQDIE--FNQVLNITYLNPRKHRHIPKAPDGVLMPKKILKA 363
Query: 66 VQYVYMPILSQQDYHLGRLQ--------------------RLVEDYVHVVADKYPYWNRS 105
+ P+L ++ + GR Q RLV++ +++ ++ PY +
Sbjct: 364 IDIKPFPVLWHEE-NSGRRQGRERQVHGAMAGPQLGEAAHRLVKNSLNIKSNNMPYGSPE 540
Query: 106 NGADHFLLS 114
H ++
Sbjct: 541 QFPGHHAMN 567
>TC209495
Length = 444
Score = 28.1 bits (61), Expect = 0.62
Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Frame = +2
Query: 10 YEEGEQPLVHDGPLKGLYS---IEGQFMDEIDSCRKSPFKARHPDEANLFLIPFSISNIV 66
Y GE+P+ +DG G + +EG F DS ++ + R+ ++F I + + NI+
Sbjct: 209 YHYGERPIYNDGTFNGNGNGAHVEGGF----DSSTRNYYGRRYGY*LSVFDI*YMLGNII 376
Query: 67 QYVYM 71
YV +
Sbjct: 377 *YVML 391
>TC219697
Length = 523
Score = 27.3 bits (59), Expect = 1.1
Identities = 13/63 (20%), Positives = 30/63 (46%)
Frame = +3
Query: 55 LFLIPFSISNIVQYVYMPILSQQDYHLGRLQRLVEDYVHVVADKYPYWNRSNGADHFLLS 114
LF+I +S++V +P+ ++ G++ +E+ V+ ++ +W + L
Sbjct: 66 LFVILLGLSHLVCLKAVPVTRTENLMQGQVHLTLENNHKVITERNLHWEEPTITERMELE 245
Query: 115 CHD 117
HD
Sbjct: 246 LHD 254
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.142 0.448
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,211,503
Number of Sequences: 63676
Number of extensions: 82453
Number of successful extensions: 368
Number of sequences better than 10.0: 66
Number of HSP's better than 10.0 without gapping: 362
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 362
length of query: 117
length of database: 12,639,632
effective HSP length: 93
effective length of query: 24
effective length of database: 6,717,764
effective search space: 161226336
effective search space used: 161226336
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0030.13