BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.4
(236 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI700975 35 0.030
TC218690 similar to UP|O81812 (O81812) Auxilin-like protein, par... 35 0.039
TC216648 weakly similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, pa... 35 0.039
TC232956 similar to UP|Q8SKI1 (Q8SKI1) ATPase subunit 6 (Fragmen... 34 0.051
TC215669 similar to UP|Q9FMU9 (Q9FMU9) Similarity to ATFP3, part... 33 0.15
TC233817 33 0.15
TC224567 similar to PIR|T51159|T51159 HMG protein [imported] - A... 32 0.25
TC216950 32 0.25
TC218693 similar to UP|Q94KA8 (Q94KA8) BZIP transcription factor... 32 0.33
TC207027 weakly similar to UP|Q9LW95 (Q9LW95) KED, partial (17%) 32 0.33
TC213008 weakly similar to UP|Q94AA3 (Q94AA3) AT3g09210/F3L24_8,... 32 0.33
CF922292 30 0.73
TC228728 weakly similar to UP|Q84TD4 (Q84TD4) At2g17540, partial... 30 0.96
TC214485 similar to UP|O23144 (O23144) Proton pump interactor (A... 30 0.96
TC218902 30 0.96
TC219297 similar to UP|Q9FMU9 (Q9FMU9) Similarity to ATFP3, part... 30 1.2
TC206975 weakly similar to GB|AAC51654.2|9280816|HSU90236 myosin... 30 1.2
TC211133 weakly similar to UP|Q9BKV7 (Q9BKV7) Ppg3, partial (4%) 29 1.6
TC221722 29 1.6
AW233793 weakly similar to GP|27450574|gb pollen-specific calmod... 29 1.6
>BI700975
Length = 422
Score = 35.0 bits (79), Expect = 0.030
Identities = 26/87 (29%), Positives = 37/87 (41%)
Frame = +3
Query: 122 LLPHLKGVEIDKACAQLKLEEAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQE 181
L PHLK + K C +L+ ++L AE A + + E A + ++ E
Sbjct: 30 LYPHLKSDDASKRCCIEQLKRDADENAKLLLHAEERRNAAFLKSESCEGDKATLLARIAE 209
Query: 182 ADEVLAKDKAARESAEASDEEKEEEAK 208
DE L RE EA + EEE K
Sbjct: 210 LDETLR--LKTREIEEAETKRIEEEGK 284
>TC218690 similar to UP|O81812 (O81812) Auxilin-like protein, partial (48%)
Length = 1043
Score = 34.7 bits (78), Expect = 0.039
Identities = 33/139 (23%), Positives = 51/139 (35%)
Frame = +3
Query: 85 KKSPLVDEREAKRQKSDASKGTLAVNQALAAASCATGLLPHLKGVEIDKACAQLKLEEAK 144
+K L EAK +K + K LAV +A+ A + +++A A+ + +
Sbjct: 3 EKEQLKKLAEAKEKKREREKEKLAVERAIREAR-ERAFADAKERATLERAAAEARQKNIS 179
Query: 145 AAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVLAKDKAARESAEASDEEKE 204
RL A ++ K E L R ++ A A A A +
Sbjct: 180 DGRERLGKTTSQANEKTPAEKAAMEAKLKAERAAVERA--------TAEARARAIERALS 335
Query: 205 EEAKGEGVKKADVKVGGSG 223
E A E K+D V G G
Sbjct: 336 ERAASEARNKSDKSVAGFG 392
>TC216648 weakly similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (41%)
Length = 2064
Score = 34.7 bits (78), Expect = 0.039
Identities = 26/95 (27%), Positives = 45/95 (47%), Gaps = 1/95 (1%)
Frame = +1
Query: 143 AKAAYSRLKSDAENAAKRAEIAKKQAEDT-LAGVRKQLQEADEVLAKDKAARESAEASDE 201
AK+ + ++ A ++E A +D AG ++ +EAD K + E E DE
Sbjct: 172 AKSEIPEKDASSDAVAAKSENANAGDKDGGAAGNQEVEEEADAAEEKKEEIDEDGEEKDE 351
Query: 202 EKEEEAKGEGVKKADVKVGGSGEVNQEEQAKNDQV 236
++E+E + E +K K G G + ++K D V
Sbjct: 352 QEEKEEEDEAEEKEKEKAKGKG---KRRRSKKDPV 447
>TC232956 similar to UP|Q8SKI1 (Q8SKI1) ATPase subunit 6 (Fragment), partial
(9%)
Length = 915
Score = 34.3 bits (77), Expect = 0.051
Identities = 38/159 (23%), Positives = 65/159 (39%), Gaps = 8/159 (5%)
Frame = +1
Query: 63 ASQVETAKGHRKNEKKETKGVRKKSPLVDEREA--KRQKSDASKGTLAVNQALAAASCAT 120
A +++ + K K+ +K+ +VDE K+ K+D K +L N + +T
Sbjct: 124 ADDLDSGRKSSKKRKRTVDASKKEDRVVDEENKGHKKDKADRKKESLITNVDPGMENKST 303
Query: 121 GLLPHLKGVEIDKACAQLKLEE-AKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRK-- 177
+DK A LEE +LK +N + AE + A + K
Sbjct: 304 KKKAKKDDKPLDKGFA---LEENVSKVEKKLKKQHKNGEQSELDVIDDAEASFAELFKIN 474
Query: 178 ---QLQEADEVLAKDKAARESAEASDEEKEEEAKGEGVK 213
L ADE+ A+DKA ++ + + + AK + K
Sbjct: 475 DAENLNLADEMKAQDKAPKDVMKLAGDPVTSFAKHKNAK 591
>TC215669 similar to UP|Q9FMU9 (Q9FMU9) Similarity to ATFP3, partial (54%)
Length = 1305
Score = 32.7 bits (73), Expect = 0.15
Identities = 34/145 (23%), Positives = 63/145 (43%), Gaps = 7/145 (4%)
Frame = +2
Query: 73 RKNEKKETKGVRKKSPLVDEREAKRQKSDASKGTLAVNQALAAASCATGL---LPHLKGV 129
+ E+K+ + K P +E+ K +A + + +C+ + + +KGV
Sbjct: 518 KPQEEKKVQEEEKPKPTPEEK-----KEEAQIVMTVLKVGMHCEACSQEIKRRIQRMKGV 682
Query: 130 EIDKACAQLKLEEAKAAYSRLKSDA---ENAAKRAEIAKKQAEDTLAGVRKQLQEA-DEV 185
E + Q K Y K + K A I K++ E +++++EA +E
Sbjct: 683 ESAEPDLQNSQVSVKGVYDPAKLVEYVYKRTGKHAVIVKQEPEK-----KEKVEEAKEEK 847
Query: 186 LAKDKAARESAEASDEEKEEEAKGE 210
++K + E + E+KEEEAK E
Sbjct: 848 KEEEKKSGGEGEENKEKKEEEAKVE 922
>TC233817
Length = 493
Score = 32.7 bits (73), Expect = 0.15
Identities = 24/76 (31%), Positives = 38/76 (49%)
Frame = +2
Query: 144 KAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVLAKDKAARESAEASDEEK 203
K+A S K N K E A + + + KQL+EA E ++A + S + S EK
Sbjct: 86 KSASSARKDSIRNRRKTVENANLASLKHME-LEKQLEEACEAEDFERAEKVSDDLSAAEK 262
Query: 204 EEEAKGEGVKKADVKV 219
E++A +++AD V
Sbjct: 263 EKQAFANSLREADAFV 310
>TC224567 similar to PIR|T51159|T51159 HMG protein [imported] - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (71%)
Length = 876
Score = 32.0 bits (71), Expect = 0.25
Identities = 52/190 (27%), Positives = 71/190 (37%), Gaps = 16/190 (8%)
Frame = +2
Query: 37 LYSVFCAA--------EMVKNKGGVNP--ESMSAFIASQVETAKGHRKNEKKETKGVRKK 86
L S+FC+ + K KG P ES+ +V K K EK KK
Sbjct: 83 LCSLFCSVSRTGT*TMKTAKGKGAARPSKESLKPVDDRKVGKRKASGKPEKSRAPKKEKK 262
Query: 87 SPLVDEREAKRQKS------DASKGTLAVNQALAAASCATGLLPHLKGVEIDKACAQLKL 140
+ D + KR S + + T L A G K + A +
Sbjct: 263 AKK-DPNKPKRPPSAFFVFLEEFRKTFKAENPLVKAVSVVGKAGGEKWKSLSSA--EKAP 433
Query: 141 EEAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVLAKDKAARESAEASD 200
EAKAA + K++ E K E KKQA A + + EV +D A+ E D
Sbjct: 434 YEAKAA--KRKAEYEKLIKAYE--KKQASS--ADDDESDKSKSEVNDEDDASGEEDHQED 595
Query: 201 EEKEEEAKGE 210
E+ EEE E
Sbjct: 596 EDDEEEEDDE 625
>TC216950
Length = 873
Score = 32.0 bits (71), Expect = 0.25
Identities = 26/78 (33%), Positives = 42/78 (53%), Gaps = 3/78 (3%)
Frame = +2
Query: 136 AQLKL---EEAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVLAKDKAA 192
A+LKL E AK ++ E + K E+ + + E L RK+L DEV A+ +
Sbjct: 215 AELKLIEEETAKRVEEAIRKRVEESLKSEEV-QVEIERRLEEGRKRLN--DEVAAQLEKE 385
Query: 193 RESAEASDEEKEEEAKGE 210
+E+A ++KEE+A+ E
Sbjct: 386 KEAALIEAKQKEEQARKE 439
>TC218693 similar to UP|Q94KA8 (Q94KA8) BZIP transcription factor 2, partial
(61%)
Length = 1226
Score = 31.6 bits (70), Expect = 0.33
Identities = 22/82 (26%), Positives = 40/82 (47%)
Frame = +3
Query: 82 GVRKKSPLVDEREAKRQKSDASKGTLAVNQALAAASCATGLLPHLKGVEIDKACAQLKLE 141
GV+ + L DERE KRQ+ S A L + L + ++ + A + ++
Sbjct: 414 GVQSQVWLQDERELKRQRRKQSNRESARRSRLRKQAECDELAQRAEALKEENATLRSEVS 593
Query: 142 EAKAAYSRLKSDAENAAKRAEI 163
+ ++ Y +L+S ENAA + +
Sbjct: 594 QIRSEYEQLRS--ENAALKERL 653
>TC207027 weakly similar to UP|Q9LW95 (Q9LW95) KED, partial (17%)
Length = 1005
Score = 31.6 bits (70), Expect = 0.33
Identities = 24/107 (22%), Positives = 48/107 (44%), Gaps = 3/107 (2%)
Frame = +3
Query: 132 DKACAQLKLEEAKAAYSRLKS---DAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVLAK 188
+K + K ++ K ++K D ++ ++ E KK + V+ + + + K
Sbjct: 141 EKKKKEKKDKDGKTDAEKIKKKEDDGKDDEEKKEKKKKYDKIDAXKVKGKEDDGKDEGNK 320
Query: 189 DKAARESAEASDEEKEEEAKGEGVKKADVKVGGSGEVNQEEQAKNDQ 235
+K +E + EEK+E+ E KK + K +E+ KND+
Sbjct: 321 EKKDKEKGDGDGEEKKEKKDKEKEKKKEKKDKDEETDTLKEKGKNDE 461
>TC213008 weakly similar to UP|Q94AA3 (Q94AA3) AT3g09210/F3L24_8, partial
(17%)
Length = 422
Score = 31.6 bits (70), Expect = 0.33
Identities = 24/65 (36%), Positives = 29/65 (43%), Gaps = 7/65 (10%)
Frame = +3
Query: 174 GVRKQLQEADEVLAKDKAARESAEASDEEKEEEAKGEG-------VKKADVKVGGSGEVN 226
GVR Q A E ARE E DEE+ EE +G G ++A+ V G E
Sbjct: 168 GVRVAAQRARE------EAREEREEGDEERREELEGGGGGEAHGKAQEAEGLVDGRAEPR 329
Query: 227 QEEQA 231
Q QA
Sbjct: 330 QSRQA 344
>CF922292
Length = 722
Score = 30.4 bits (67), Expect = 0.73
Identities = 24/95 (25%), Positives = 45/95 (47%), Gaps = 10/95 (10%)
Frame = -2
Query: 151 KSDAENAAKRAEIAKKQAEDTLAGVRK-------QLQEADEVLAK---DKAARESAEASD 200
KSD ++ K +E K + +DT ++ Q ++DE K D++ ++S A
Sbjct: 307 KSDEKSQEKPSEDTKTENQDTSVSEKRSDSDESQQKSDSDESQQKSDSDESEKKSDSAES 128
Query: 201 EEKEEEAKGEGVKKADVKVGGSGEVNQEEQAKNDQ 235
E+K + + E +D + S E N +Q +D+
Sbjct: 127 EKKSDSDESEKKSDSD-ETEKSSESNDNKQFDSDE 26
>TC228728 weakly similar to UP|Q84TD4 (Q84TD4) At2g17540, partial (25%)
Length = 1093
Score = 30.0 bits (66), Expect = 0.96
Identities = 20/62 (32%), Positives = 29/62 (46%)
Frame = +3
Query: 129 VEIDKACAQLKLEEAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVLAK 188
VE KA A + AKAA + K AE AAK AK+ + + + + + L K
Sbjct: 396 VEASKAAAVAASKAAKAARAAAKEKAETAAKAVAAAKRALDLVSSFSEEAVSSKERNLKK 575
Query: 189 DK 190
+K
Sbjct: 576 NK 581
>TC214485 similar to UP|O23144 (O23144) Proton pump interactor
(AT4g27500/F27G19_100), partial (33%)
Length = 1501
Score = 30.0 bits (66), Expect = 0.96
Identities = 20/75 (26%), Positives = 40/75 (52%)
Frame = +1
Query: 162 EIAKKQAEDTLAGVRKQLQEADEVLAKDKAARESAEASDEEKEEEAKGEGVKKADVKVGG 221
++ + + E+ +A ++ L+ + +KAA ++A + +E E++ K KKA GG
Sbjct: 745 KLKEMKREEEIAKAKQALERKKK--QAEKAAAKAATRAQKEAEKKLKVRE-KKAKKVAGG 915
Query: 222 SGEVNQEEQAKNDQV 236
+G V E+ N+ V
Sbjct: 916 TGAVPSPEEPANEAV 960
Score = 30.0 bits (66), Expect = 0.96
Identities = 49/172 (28%), Positives = 69/172 (39%), Gaps = 25/172 (14%)
Frame = +1
Query: 67 ETAKGHRKNEKKETKGVRKKSPLVDEREAKRQKSDASKGT---LAVNQALAAASCATGLL 123
ET + + K+ + K SP E K QK KG +N+ L L
Sbjct: 529 ETDTLPKSSVKQPKEEEPKSSPPETVPEQKIQKETKKKGKDLKSNLNKVLEDTDEYEFEL 708
Query: 124 PHL-KGVEID-KACAQLKLEE----AKAAYSRLKSDAENAA-KRAEIAKKQAEDTLAGVR 176
P + K ID + ++K EE AK A R K AE AA K A A+K+AE L
Sbjct: 709 PKVNKEPPIDPEKLKEMKREEEIAKAKQALERKKKQAEKAAAKAATRAQKEAEKKLKVRE 888
Query: 177 KQLQEA---------------DEVLAKDKAARESAEASDEEKEEEAKGEGVK 213
K+ ++ + V ++ +S EAS KE+ K VK
Sbjct: 889 KKAKKVAGGTGAVPSPEEPANEAVEVAEQKTDDSMEASAPAKEKVPKESSVK 1044
>TC218902
Length = 1098
Score = 30.0 bits (66), Expect = 0.96
Identities = 46/217 (21%), Positives = 82/217 (37%), Gaps = 48/217 (22%)
Frame = +2
Query: 64 SQVETAKGHRKNEKKETKGVRKKSPLVDERE------AKRQKSDASKGTLAVNQALAAAS 117
S++ + K K++ + KK L E A+ + + K QA A S
Sbjct: 86 SEIALVQMKEKEAKEKMTELPKKLQLTAEETNQANLLAQAAREELQKVKAEAEQAKAGVS 265
Query: 118 CATG-LLPHLKGVEIDKACAQLK------LEEAKAAYSRLKSDAENA-----------AK 159
LL K +E KA L L+E+++ S+ + D N +K
Sbjct: 266 TLESRLLAAQKEIEAAKASENLAIAAIKALQESESTRSKNEVDPSNGVTLSLEEYYELSK 445
Query: 160 RAEIAKKQAEDTLAGVRKQL-----------QEADEVLAKDKAARESAEASDEEKEEEAK 208
RA A+++A +A ++ ++ DEV + A RES + + E+ E+ +
Sbjct: 446 RAHEAEERANMRVAAANSEIDKVKESELKAFEKLDEVNREIAARRESLKLAMEKAEKAKE 625
Query: 209 G-------------EGVKKADVKVGGSGEVNQEEQAK 232
G E ++ G G +NQ + +
Sbjct: 626 GKLGVEQELRKWRAESEQRRKAGESGQGVINQSKSPR 736
>TC219297 similar to UP|Q9FMU9 (Q9FMU9) Similarity to ATFP3, partial (30%)
Length = 792
Score = 29.6 bits (65), Expect = 1.2
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 12/98 (12%)
Frame = +1
Query: 133 KACAQL------KLEEAKAAYSRLKSDAE------NAAKRAEIAKKQAEDTLAGVRKQLQ 180
+ACAQ+ K++ ++ + L +D + AK + K+ + + V+++ +
Sbjct: 100 EACAQVIQKRIRKIQGVESVETSLGNDQVIVKGVIDPAKLVDYVYKRTKKQASIVKEEEK 279
Query: 181 EADEVLAKDKAARESAEASDEEKEEEAKGEGVKKADVK 218
E K + + E EEKEEE KGE ++ D K
Sbjct: 280 E------KKEEEEKKEEEKKEEKEEEKKGEDGEEVDTK 375
>TC206975 weakly similar to GB|AAC51654.2|9280816|HSU90236 myosin VI {Homo
sapiens;} , partial (3%)
Length = 827
Score = 29.6 bits (65), Expect = 1.2
Identities = 29/100 (29%), Positives = 45/100 (45%), Gaps = 7/100 (7%)
Frame = +3
Query: 137 QLKLEEAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVL------AKDK 190
Q LEE+ + + DAE + ++Q A R L EAD+ +++
Sbjct: 48 QRVLEESSPVLAAARLDAEERRNNMRLREEQD----AAYRAAL-EADQAR*RQRREEEER 212
Query: 191 AARESAEASDEEKEEEAKGE-GVKKADVKVGGSGEVNQEE 229
A E+AEA + KEEEA E ++A K + QE+
Sbjct: 213 LAWEAAEAERKRKEEEAARERSAQEAAEKQAALANIRQEK 332
>TC211133 weakly similar to UP|Q9BKV7 (Q9BKV7) Ppg3, partial (4%)
Length = 709
Score = 29.3 bits (64), Expect = 1.6
Identities = 30/86 (34%), Positives = 38/86 (43%), Gaps = 14/86 (16%)
Frame = -3
Query: 161 AEIAKKQAEDTLAGVRKQLQEADEVLAKDKAARESAEASDEEKEE----------EAKGE 210
AE Q E L G+ + L A AA E E EE+EE E +GE
Sbjct: 386 AEALNLQIE--LHGLLEALASLALAAAGSGAAEEGGEGEGEEEEEGEEVGEGEGGEEEGE 213
Query: 211 GVKKADVKVGG----SGEVNQEEQAK 232
GV+ +VGG +GE +QEE K
Sbjct: 212 GVEDV-AEVGGEEEGAGEEDQEED*K 138
>TC221722
Length = 716
Score = 29.3 bits (64), Expect = 1.6
Identities = 33/119 (27%), Positives = 51/119 (42%), Gaps = 7/119 (5%)
Frame = +1
Query: 85 KKSPLVDE------REAKRQKSDASKGTL-AVNQALAAASCATGLLPHLKGVEIDKACAQ 137
K++PL+ + R KRQ S S+G +V +A AA A + A
Sbjct: 262 KRAPLIPKPLLRKRRSFKRQPSQFSRGNQPSVLKAFAAEQDAM------------EENAM 405
Query: 138 LKLEEAKAAYSRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEADEVLAKDKAARESA 196
LK+EEAKA + A N KRA+ + A+ ++ A+ LA + +A
Sbjct: 406 LKIEEAKANANMSMELAINKRKRAQSLAQNADLATYKATMLIRIAEAALAAESVDEAAA 582
>AW233793 weakly similar to GP|27450574|gb pollen-specific calmodulin-binding
protein {Arabidopsis thaliana}, partial (16%)
Length = 393
Score = 29.3 bits (64), Expect = 1.6
Identities = 29/96 (30%), Positives = 43/96 (44%), Gaps = 4/96 (4%)
Frame = +2
Query: 92 EREAKRQKSDASKGTL----AVNQALAAASCATGLLPHLKGVEIDKACAQLKLEEAKAAY 147
ERE +R++ S+ VN A A S A G HLKGV + + ++AK A
Sbjct: 2 ERERERERESCSEDPYLAAEGVNHAQRAISNAHGPNEHLKGVAL-RMLGLCLGKQAKVA- 175
Query: 148 SRLKSDAENAAKRAEIAKKQAEDTLAGVRKQLQEAD 183
SD E R+ + K E +A +R + +D
Sbjct: 176 ---SSDFE----RSHLQSKALESLVAAIRLEPNNSD 262
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.307 0.123 0.319
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,914,589
Number of Sequences: 63676
Number of extensions: 65908
Number of successful extensions: 512
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of query: 236
length of database: 12,639,632
effective HSP length: 94
effective length of query: 142
effective length of database: 6,654,088
effective search space: 944880496
effective search space used: 944880496
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0023.4