BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.13
(195 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218847 87 4e-18
TC206673 83 9e-17
TC213164 68 2e-12
BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase... 58 2e-09
BI321779 55 2e-08
TC234193 42 2e-04
CA851030 40 7e-04
BI968991 34 0.038
TC234374 33 0.11
TC212583 32 0.14
BG405621 31 0.32
TC212718 similar to UP|Q91CY9 (Q91CY9) ORF1, partial (4%) 31 0.41
TC215480 similar to GB|BAA96951.1|8777361|AB019233 protein carbo... 30 0.92
TC215477 weakly similar to UP|Q8DYS1 (Q8DYS1) PAP2 family protei... 30 0.92
BE474579 29 1.6
TC225670 UP|P93167 (P93167) Asparagine synthetase 2 , complete 27 4.6
TC219840 similar to UP|Q9M2B4 (Q9M2B4) Nucleic acid binding prot... 27 4.6
TC209703 weakly similar to UP|Q43701 (Q43701) Heat shock protein... 27 7.8
CA800510 27 7.8
>TC218847
Length = 521
Score = 87.4 bits (215), Expect = 4e-18
Identities = 42/95 (44%), Positives = 63/95 (66%)
Frame = -3
Query: 36 WTVLRLHE*LLYQKSRSKWLKEGDQNTKYFHSLINWRKRTNSIMGLMIDGNWVEDPQAIK 95
WT + HE LL QK+R+KW++E D N+ YFH LIN+ RTN++ G+MI G+WVE+P +K
Sbjct: 282 WTAAQAHESLLRQKARTKWIRERDCNSSYFHKLINYIHRTNAVNGVMIGGSWVEEPNRVK 103
Query: 96 TEVQNYFEQCFTNSLGTLVRLDEISFKSISDADNE 130
V+N+F+ F+ S ++ +F+SI NE
Sbjct: 102 A-VRNFFQIRFSESDYDRP*INGANFRSIGQQQNE 1
>TC206673
Length = 742
Score = 82.8 bits (203), Expect = 9e-17
Identities = 37/90 (41%), Positives = 55/90 (61%)
Frame = +3
Query: 2 NEVVQKLNELDRKEEEEGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQN 61
+ + +KLNE++ LS +E +K L + W +E LL QKSR KWLKEGD N
Sbjct: 24 HNIKKKLNEVEDLASNRSLSVDEIKAKKELQQQLWEASTAYESLLRQKSRDKWLKEGDNN 203
Query: 62 TKYFHSLINWRKRTNSIMGLMIDGNWVEDP 91
+ YFH++IN+R+ N + G++I G W+ P
Sbjct: 204 SAYFHTVINFRRHYNGLQGILIQGEWIALP 293
>TC213164
Length = 446
Score = 68.2 bits (165), Expect = 2e-12
Identities = 30/62 (48%), Positives = 42/62 (67%)
Frame = +3
Query: 129 NELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKFIQKFWDLLKDDFHRVLEDFWNRGCG 188
++LL RF +EV+ +W+ G KSP PD NFKFI++FWD+LK D R L++F+ G
Sbjct: 9 SDLLVARFEKKEVRATMWDRGNAKSPGPDGLNFKFIKEFWDILKTDLLRFLDEFYANGVF 188
Query: 189 LK 190
LK
Sbjct: 189 LK 194
>BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (15%)
Length = 376
Score = 58.2 bits (139), Expect = 2e-09
Identities = 25/46 (54%), Positives = 30/46 (64%)
Frame = -2
Query: 141 VKGAVWECGGDKSPEPDEFNFKFIQKFWDLLKDDFHRVLEDFWNRG 186
+K AVW+CG KSP PD NFKFI+ FW+ LK D R L +F G
Sbjct: 375 IKSAVWQCGSVKSPGPDGLNFKFIKHFWERLKPDIIRFLNEFHANG 238
>BI321779
Length = 421
Score = 55.5 bits (132), Expect = 2e-08
Identities = 24/78 (30%), Positives = 44/78 (55%)
Frame = +2
Query: 105 CFTNSLGTLVRLDEISFKSISDADNELLTGRFGMEEVKGAVWECGGDKSPEPDEFNFKFI 164
CF + +L+ + F S S+ DN +L F +EE+K + +C KSP PD ++ I
Sbjct: 2 CFQEQIYGRPKLNGVEFLSFSNEDNAMLIQDFEVEEIKKVI*DCESSKSPGPDGYSLLLI 181
Query: 165 QKFWDLLKDDFHRVLEDF 182
+ FW+++K++ ++F
Sbjct: 182 KIFWEVIKEEVINFFKEF 235
>TC234193
Length = 697
Score = 42.0 bits (97), Expect = 2e-04
Identities = 25/79 (31%), Positives = 46/79 (57%), Gaps = 5/79 (6%)
Frame = -3
Query: 114 VRLDEISFKSISDADNELLTGRF--GMEEVKGAV---WECGGDKSPEPDEFNFKFIQKFW 168
V LD ++FK+I+ DN+ LT G + +G++ W+ D + +NF FI+K
Sbjct: 326 VLLDGVNFKTINGEDNDRLTHVLVLGTQNKRGSLGV*WKQKFDSNG----YNFNFIKKC* 159
Query: 169 DLLKDDFHRVLEDFWNRGC 187
+++K+D R +++F + GC
Sbjct: 158 EVMKEDVVRAIQEFHSHGC 102
>CA851030
Length = 358
Score = 40.0 bits (92), Expect = 7e-04
Identities = 23/60 (38%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
Frame = +1
Query: 37 TVLRLHE*LLYQKSRSKWLKEGDQNTKYFHSLINWRKRTNSIMGLMID-GNWVEDPQAIK 95
++L+ E Q+SR+ W KE D+NT++FH RK +NSI + D GN P I+
Sbjct: 157 SLLKQEEEYWQQRSRAIWFKERDKNTEFFHRKTLQRKASNSITKIQDDEGNVCFKPDQIE 336
>BI968991
Length = 750
Score = 34.3 bits (77), Expect = 0.038
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = +1
Query: 45 LLYQKSRSKWLKEGDQNTKYFHSL 68
L ++KS +KW+K+GD T YFH L
Sbjct: 25 LWFRKS*AKWIKDGDHETGYFHGL 96
>TC234374
Length = 787
Score = 32.7 bits (73), Expect = 0.11
Identities = 24/67 (35%), Positives = 35/67 (51%), Gaps = 6/67 (8%)
Frame = +2
Query: 9 NELDRKEEE---EGLSEEENNERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQNTKYF 65
NE+D +++ G S+ N+ E TVLR E L +KSR +G+QNT +F
Sbjct: 167 NEVDSIQQQIVNVGYSDALFNQEVLAQKELQTVLRFQECLWREKSRI*GHAQGEQNTSFF 346
Query: 66 ---HSLI 69
HS+I
Sbjct: 347 IK*HSII 367
>TC212583
Length = 530
Score = 32.3 bits (72), Expect = 0.14
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2
Query: 53 KWLKEGDQNTKYFHSLIN 70
KW EGD N+K+FHS +N
Sbjct: 2 KWFLEGDHNSKFFHSYVN 55
>BG405621
Length = 402
Score = 31.2 bits (69), Expect = 0.32
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +2
Query: 48 QKSRSKWLKEGDQNTKYFHS 67
Q+S+ WLK GD N+K+FHS
Sbjct: 341 QRSKIFWLKYGDLNSKFFHS 400
>TC212718 similar to UP|Q91CY9 (Q91CY9) ORF1, partial (4%)
Length = 428
Score = 30.8 bits (68), Expect = 0.41
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = +2
Query: 11 LDRKEEEEGLSEEENNERKWLLDE 34
L R E EEGLS EE +WL+DE
Sbjct: 347 LQRGEYEEGLSVEEAENARWLVDE 418
>TC215480 similar to GB|BAA96951.1|8777361|AB019233 protein carboxyl
methylase-like {Arabidopsis thaliana;} , partial (21%)
Length = 955
Score = 29.6 bits (65), Expect = 0.92
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +1
Query: 12 DRKEEEEGLSEEENNERKWLLDEFWTV 38
+++ EE EE+ ++KWL +WTV
Sbjct: 172 EKRRSEEPKKEEKKKKQKWLNQNYWTV 252
>TC215477 weakly similar to UP|Q8DYS1 (Q8DYS1) PAP2 family protein, partial
(11%)
Length = 1180
Score = 29.6 bits (65), Expect = 0.92
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -1
Query: 12 DRKEEEEGLSEEENNERKWLLDEFWTV 38
+++ EE EE+ ++KWL +WTV
Sbjct: 451 EKRRSEEPKKEEKKKKQKWLNQNYWTV 371
>BE474579
Length = 419
Score = 28.9 bits (63), Expect = 1.6
Identities = 19/58 (32%), Positives = 32/58 (54%)
Frame = +3
Query: 33 DEFWTVLRLHE*LLYQKSRSKWLKEGDQNTKYFHSLINWRKRTNSIMGLMIDGNWVED 90
+EFW V + + *+L+ K + + F ++N RTN+IMGL+++ W ED
Sbjct: 3 EEFWKVSKWYI*ILFSTKIKKQVN*S*G--RQFVHMMN*-SRTNAIMGLLMEECWEED 167
>TC225670 UP|P93167 (P93167) Asparagine synthetase 2 , complete
Length = 2130
Score = 27.3 bits (59), Expect = 4.6
Identities = 15/35 (42%), Positives = 19/35 (53%)
Frame = +3
Query: 34 EFWTVLRLHE*LLYQKSRSKWLKEGDQNTKYFHSL 68
E WT+L LH LL + L GDQ+ Y HS+
Sbjct: 855 EVWTLLWLHPSLLVTWPTQRLLSSGDQS--YIHSV 953
>TC219840 similar to UP|Q9M2B4 (Q9M2B4) Nucleic acid binding protein-like,
partial (41%)
Length = 622
Score = 27.3 bits (59), Expect = 4.6
Identities = 14/36 (38%), Positives = 19/36 (51%), Gaps = 10/36 (27%)
Frame = +3
Query: 11 LDRKEEEEGLSEEENNE----------RKWLLDEFW 36
L K+E+EGL EE+N+E + DEFW
Sbjct: 105 LQPKDEDEGLEEEDNDEHGDTLCGACSENYGTDEFW 212
>TC209703 weakly similar to UP|Q43701 (Q43701) Heat shock protein 17.2,
partial (51%)
Length = 421
Score = 26.6 bits (57), Expect = 7.8
Identities = 14/37 (37%), Positives = 19/37 (50%), Gaps = 10/37 (27%)
Frame = +2
Query: 142 KGAVWECGGDKSPEPDEFN----------FKFIQKFW 168
KGAVW GG++S ++ N KF +KFW
Sbjct: 287 KGAVWTXGGERSRXKEDKNEKGXRVERGRGKFQRKFW 397
>CA800510
Length = 409
Score = 26.6 bits (57), Expect = 7.8
Identities = 15/44 (34%), Positives = 18/44 (40%)
Frame = -2
Query: 27 ERKWLLDEFWTVLRLHE*LLYQKSRSKWLKEGDQNTKYFHSLIN 70
+R EFW R E L+QK KW+ G F S N
Sbjct: 192 QRSVFQQEFWAFTRQKESSLFQKWHPKWIN*GTTILNIFISA*N 61
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.139 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,851,737
Number of Sequences: 63676
Number of extensions: 135207
Number of successful extensions: 750
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 694
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 724
length of query: 195
length of database: 12,639,632
effective HSP length: 92
effective length of query: 103
effective length of database: 6,781,440
effective search space: 698488320
effective search space used: 698488320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0023.13