BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0020.10
(535 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229837 weakly similar to UP|Q9LWT7 (Q9LWT7) ESTs AU082452(S233... 298 4e-81
AW830219 weakly similar to GP|7248400|dbj| ESTs AU082452(S2330) ... 198 4e-51
BG404972 60 3e-09
TC233431 homologue to GB|CAA35302.1|1780922|HEHCMVCG HCMVIRL8 {H... 35 0.11
TC220631 weakly similar to UP|O80638 (O80638) Nodulin-like prote... 34 0.14
TC218179 31 1.2
TC209482 31 1.6
AI495930 30 2.7
TC227779 similar to UP|Q8VWZ8 (Q8VWZ8) Sterol 4-alpha-methyl-oxi... 30 2.7
TC216336 similar to GB|AAO42967.1|28416873|BT004721 At5g19875 {A... 30 2.7
TC227074 homologue to UP|PP1_PHAVU (P48490) Serine/threonine pro... 29 4.6
TC216670 weakly similar to UP|Q39364 (Q39364) Non-green plastid ... 29 4.6
TC229177 weakly similar to UP|Q21086 (Q21086) C. elegans NST-1 p... 29 6.0
BQ612416 29 6.0
CF922531 29 6.0
CD487594 weakly similar to GP|16323149|gb| AT4g10360/F24G24_160 ... 29 6.0
TC208184 UP|Q39834 (Q39834) Clathrin heavy chain, complete 29 6.0
TC206859 similar to UP|Q9SB50 (Q9SB50) Clathrin coat assembly li... 29 6.0
TC225830 similar to GB|AAL49942.1|17979251|AY070476 AT3g53990/F5... 29 6.0
BQ629867 28 7.8
>TC229837 weakly similar to UP|Q9LWT7 (Q9LWT7) ESTs AU082452(S2330), partial
(15%)
Length = 827
Score = 298 bits (763), Expect = 4e-81
Identities = 147/182 (80%), Positives = 160/182 (87%), Gaps = 1/182 (0%)
Frame = +3
Query: 355 KPVHLLFFWLTYFLFPVFSLSLLQPLLRVIKMNSVKMQIVGFLFSLISSGFGFYYGHSHW 414
K +HLL+ LTYFL + S LLQPLL VIKMNSVKMQIVGFL SL+SSGFGFYYGHSHW
Sbjct: 3 KXLHLLYLXLTYFLXXLXSXXLLQPLLHVIKMNSVKMQIVGFLLSLLSSGFGFYYGHSHW 182
Query: 415 KWGHLVVFSVIQSTGTGILHAFSRVLVLDCAPSGKEGAFSIWYAWMRSAGLLVGFTVGSV 474
KWGHLV+F +QST TGIL+AF R+LVLDCAPSGKEGAFSIWYAWMR+AGL VGFTVGSV
Sbjct: 183 KWGHLVLFGAVQSTATGILYAFGRLLVLDCAPSGKEGAFSIWYAWMRAAGLCVGFTVGSV 362
Query: 475 SPGNIRTSFGAAFCTAIAGIVVLLFGNISDVGGAVAAGHVRDDSERSSAV-AGLDTKESV 533
+PG +RTSFG AFCTAIAGIVVLLFGNISDVGGAVAAGHVRD+ ERSSAV GLD+KES
Sbjct: 363 APGRVRTSFGVAFCTAIAGIVVLLFGNISDVGGAVAAGHVRDECERSSAVLPGLDSKESA 542
Query: 534 HV 535
V
Sbjct: 543 RV 548
>AW830219 weakly similar to GP|7248400|dbj| ESTs AU082452(S2330)
AU058131(S5384) D40386(S2330) D23841(R0349) correspond
to a region of the, partial (4%)
Length = 335
Score = 198 bits (504), Expect = 4e-51
Identities = 93/111 (83%), Positives = 101/111 (90%)
Frame = +1
Query: 346 VFIVGQLCIKPVHLLFFWLTYFLFPVFSLSLLQPLLRVIKMNSVKMQIVGFLFSLISSGF 405
+FIVGQLCIKP+HLL+ WLTYFLFP+ SLSLLQPLL VIKMNSVKMQIVGFL SL+SSGF
Sbjct: 1 IFIVGQLCIKPLHLLYLWLTYFLFPLVSLSLLQPLLHVIKMNSVKMQIVGFLSSLLSSGF 180
Query: 406 GFYYGHSHWKWGHLVVFSVIQSTGTGILHAFSRVLVLDCAPSGKEGAFSIW 456
GFYYGHSHWKWGHLV+F QST TGIL+AF R+LVLD APSGKEGAFSIW
Sbjct: 181 GFYYGHSHWKWGHLVLFGAAQSTATGILYAFGRLLVLD*APSGKEGAFSIW 333
>BG404972
Length = 394
Score = 59.7 bits (143), Expect = 3e-09
Identities = 34/73 (46%), Positives = 44/73 (59%), Gaps = 1/73 (1%)
Frame = +3
Query: 1 MKEVTVEAEGGGHHQGGAQGGAPENGKGTTATRPDLHNRAMKILRAREAYN-GYEEVGEK 59
MKEV+ E GGG Q + AP+NG + +MKI ++ E Y+ YEE G++
Sbjct: 198 MKEVSAEG-GGGLGQASSSVPAPQNGNS-------MQPSSMKISKSGEVYSYDYEETGDE 353
Query: 60 PSRFEVMGWYLYE 72
PSRFEV GWYLYE
Sbjct: 354 PSRFEVWGWYLYE 392
>TC233431 homologue to GB|CAA35302.1|1780922|HEHCMVCG HCMVIRL8 {Human
herpesvirus 5;} , partial (9%)
Length = 540
Score = 34.7 bits (78), Expect = 0.11
Identities = 18/67 (26%), Positives = 34/67 (49%)
Frame = -3
Query: 291 GIFHVATAIGRTTDEISFSSRLHPFSVFKYPHAIGALASVFISSFITMTIFTGGVVFIVG 350
GI VAT G E+ FSS PF K +++ L ++ + +T +F G V ++
Sbjct: 343 GIGVVATWSGGVVREVPFSSSPLPFPATKDTNSVTDLTTLLTTKIVTRPLFGGSVCLLLD 164
Query: 351 QLCIKPV 357
++ ++ +
Sbjct: 163 RVAVRSI 143
>TC220631 weakly similar to UP|O80638 (O80638) Nodulin-like protein
(At2g39510), partial (31%)
Length = 797
Score = 34.3 bits (77), Expect = 0.14
Identities = 37/132 (28%), Positives = 64/132 (48%), Gaps = 10/132 (7%)
Frame = +1
Query: 229 SQFPIRQGIS-----SKLSLHATAAGCLGSALISAFTYHMIRELNVNEPDIMSL-W---- 278
S F I Q I+ +++SL AT +G+ SA ++ M R N PD+ SL W
Sbjct: 151 SAFYILQAITLRKYPAEMSL-ATWVCFVGALQSSAVSFFMER----NSPDVWSLAWDSRL 315
Query: 279 IVSIFSGLIWLVGIFHVATAIGRTTDEISFSSRLHPFSVFKYPHAIGALASVFISSFITM 338
+ +SG++ F+V + +TT + F + +P + + ALA + +S + +
Sbjct: 316 VAYAYSGIVTSAIQFYVQGMVIKTTGPV-FVTAFNPLRMI----IVTALACIVLSEKLHL 480
Query: 339 TIFTGGVVFIVG 350
GGVV ++G
Sbjct: 481 GSIIGGVVVVIG 516
>TC218179
Length = 1135
Score = 31.2 bits (69), Expect = 1.2
Identities = 23/78 (29%), Positives = 35/78 (44%), Gaps = 4/78 (5%)
Frame = +1
Query: 397 LFSLISSGFGFYYGHSHWKWGHLVVFSVIQSTGTGILHAFSRVLVL-DCAPSGKEGAFSI 455
+ S+ SSG GFYY K G +V S + + + + +L L + PS + F +
Sbjct: 91 IISVCSSGTGFYYQTRGLKHGRAIVVSTCAAVASILTGVLAGMLALGERLPSAPKARFLL 270
Query: 456 WYAWMR---SAGLLVGFT 470
W+ LLVG T
Sbjct: 271 LLGWLLIIVGVILLVGST 324
>TC209482
Length = 921
Score = 30.8 bits (68), Expect = 1.6
Identities = 36/142 (25%), Positives = 63/142 (44%), Gaps = 2/142 (1%)
Frame = +1
Query: 172 STAVGVFFCLPAGFFKVTAIFIPYIAGIIAASTVATAAHTHHLGLMIRSFTGPTLKRSQF 231
+T +G FCL A FK + FI + + + T A +++ L +K S
Sbjct: 202 ATFLGAIFCLIAFLFKSLSGFIVFFS-MGELLIFVTQAPVNYVSLRC-------VKPSLR 357
Query: 232 PIRQGISSKLSLHATAAGCLGSALISAFTYHMIRELNVNEPDIMSLWIVSIF--SGLIWL 289
P+ IS+ +S+H S L+ H +N+ +L + SIF + +IW
Sbjct: 358 PLSMAIST-VSIHVFG-DVPSSPLVGVLQDH------INDWRKTALCLTSIFFLAAVIWF 513
Query: 290 VGIFHVATAIGRTTDEISFSSR 311
+GIF + + +E S ++R
Sbjct: 514 IGIFLKSDVYDKDDEEQSATTR 579
>AI495930
Length = 357
Score = 30.0 bits (66), Expect = 2.7
Identities = 21/63 (33%), Positives = 27/63 (42%), Gaps = 7/63 (11%)
Frame = +2
Query: 7 EAEGGGHHQGGAQGGAPENGKGT-------TATRPDLHNRAMKILRAREAYNGYEEVGEK 59
EA+ G +GG+ GG G G TAT +L+ + RA A GY GE
Sbjct: 149 EAKESGEERGGSAGGEGRGGAGPGYE*NQGTATTRELYPTSSA*ARA*AAATGYGGFGES 328
Query: 60 PSR 62
R
Sbjct: 329 ERR 337
>TC227779 similar to UP|Q8VWZ8 (Q8VWZ8) Sterol 4-alpha-methyl-oxidase,
partial (97%)
Length = 1131
Score = 30.0 bits (66), Expect = 2.7
Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 2/60 (3%)
Frame = +1
Query: 367 FLFPVFSLSLLQPLLRVIKMNSVKMQIVGFLFSLISSGFGFYYGHS--HWKWGHLVVFSV 424
F +PVF+ ++ L + S K+ ++ +F I F FY+GH H KW + V SV
Sbjct: 325 FSYPVFTYMGMRSSL---PLPSWKVVLIQIIFYFILEDFIFYWGHRILHTKWLYKHVHSV 495
>TC216336 similar to GB|AAO42967.1|28416873|BT004721 At5g19875 {Arabidopsis
thaliana;} , partial (33%)
Length = 821
Score = 30.0 bits (66), Expect = 2.7
Identities = 17/40 (42%), Positives = 26/40 (64%), Gaps = 1/40 (2%)
Frame = +2
Query: 356 PVHLLFFWLTYFLFPVFSL-SLLQPLLRVIKMNSVKMQIV 394
P+HL FF LT F+F FSL S +P++ M+ VK+ ++
Sbjct: 182 PMHLCFFLLTLFMFLSFSLYSSYEPIMESF-MDQVKLVLM 298
>TC227074 homologue to UP|PP1_PHAVU (P48490) Serine/threonine protein
phosphatase PP1 , complete
Length = 1303
Score = 29.3 bits (64), Expect = 4.6
Identities = 17/58 (29%), Positives = 29/58 (49%), Gaps = 2/58 (3%)
Frame = -3
Query: 334 SFITMTIFTGGVVFIVGQLCIKPVHLLFFWLTYFL--FPVFSLSLLQPLLRVIKMNSV 389
+FI TI+T + F + + P + F W++YF+ +SL L P + +I V
Sbjct: 737 TFIIKTIYTVYLCFFM----VSPQEINFVWISYFVGQKQTYSLQTLLPSIHIIPQKQV 576
>TC216670 weakly similar to UP|Q39364 (Q39364) Non-green plastid inner
envelope membrane protein precursor, partial (24%)
Length = 1453
Score = 29.3 bits (64), Expect = 4.6
Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = -3
Query: 137 WTSIGWAIGLAIAAPILGFLSFHLDGH-FPKFITVASTAVGVFFCLPAGFFKVTAIFIPY 195
W S G + ++ + G LS D + FP + + + FF L A F +A+F+ Y
Sbjct: 701 WISSGGEVNVSTISFTSGELSAAADKYSFPSSVISFAATLNSFFVLSASTFNCSAVFLEY 522
>TC229177 weakly similar to UP|Q21086 (Q21086) C. elegans NST-1 protein
(Corresponding sequence K01C8.9), partial (21%)
Length = 1353
Score = 28.9 bits (63), Expect = 6.0
Identities = 17/62 (27%), Positives = 28/62 (44%)
Frame = -1
Query: 143 AIGLAIAAPILGFLSFHLDGHFPKFITVASTAVGVFFCLPAGFFKVTAIFIPYIAGIIAA 202
++G A L F F +++ S A FCLPA F +F+P + I ++
Sbjct: 1077 SMGSDEATAFLAFFLFFFSARLIFVLSIPSVAYNFSFCLPASLF---PLFLPASSSIDSS 907
Query: 203 ST 204
+T
Sbjct: 906 TT 901
>BQ612416
Length = 425
Score = 28.9 bits (63), Expect = 6.0
Identities = 22/81 (27%), Positives = 36/81 (44%), Gaps = 2/81 (2%)
Frame = +2
Query: 410 GHSHWKWGHLVVFSVIQSTGTGILHAFSRVLVL--DCAPSGKEGAFSIWYAWMRSAGLLV 467
G W+W V FS+ +L A+ R L + S ++G+ +I + R+
Sbjct: 56 GAEVWRWTSFVSFSL-----NILLEAYCRALGKRRNFC*SARKGSNTIRVSANRTCYAAF 220
Query: 468 GFTVGSVSPGNIRTSFGAAFC 488
G T P N+ T++G FC
Sbjct: 221 GCTST*TWPNNVDTAYGTVFC 283
>CF922531
Length = 602
Score = 28.9 bits (63), Expect = 6.0
Identities = 15/48 (31%), Positives = 26/48 (53%)
Frame = +3
Query: 451 GAFSIWYAWMRSAGLLVGFTVGSVSPGNIRTSFGAAFCTAIAGIVVLL 498
G SIW + G G ++G++SP + ++S+ +I GIV +L
Sbjct: 156 GTKSIWCSIPLKGGSPRGISLGNISPNSCKSSWTFGGKVSIVGIVTVL 299
>CD487594 weakly similar to GP|16323149|gb| AT4g10360/F24G24_160 {Arabidopsis
thaliana}, partial (34%)
Length = 602
Score = 28.9 bits (63), Expect = 6.0
Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 12/75 (16%)
Frame = +2
Query: 237 ISSKLSLHATAAGCLGSALI--SAFTYHMIRELNVN----------EPDIMSLWIVSIFS 284
++S +H T CL L+ S F + EL + P + LW+ SIFS
Sbjct: 38 LNSGTLVHNTVMSCLRCCLV*FSKFEQKGLMELTSSFGLTFFYDYATPSKVVLWLASIFS 217
Query: 285 GLIWLVGIFHVATAI 299
G+I V + + +TAI
Sbjct: 218 GIILCV-LVYTSTAI 259
>TC208184 UP|Q39834 (Q39834) Clathrin heavy chain, complete
Length = 5624
Score = 28.9 bits (63), Expect = 6.0
Identities = 14/44 (31%), Positives = 22/44 (49%), Gaps = 1/44 (2%)
Frame = -2
Query: 98 PADWAKNNRPAGTHCSQKELHLYSKLT-NQTITSNFSALEWTSI 140
P WAK N A TH Q H+++ L T+ ++ +TS+
Sbjct: 3940 PHIWAKRNSSASTHAKQTSFHVFALLAFLAASTAPWNCFNFTSV 3809
>TC206859 similar to UP|Q9SB50 (Q9SB50) Clathrin coat assembly like protein,
complete
Length = 1644
Score = 28.9 bits (63), Expect = 6.0
Identities = 37/130 (28%), Positives = 54/130 (41%), Gaps = 6/130 (4%)
Frame = +2
Query: 354 IKPVHLLFFWLTYF-LFPVFSLSLLQPLLRVIKMNSVKMQIVGFLFSLISSGFGFYYGHS 412
+K V LLF T P F L LLQ + RVIK +L L +
Sbjct: 254 VKVVGLLFVATTRVNTSPSFVLELLQRIARVIK---------DYLGILNEDSLRKNFVLV 406
Query: 413 HWKWGHLVVFSVIQSTGTGIL--HAFSRVLVLDCA---PSGKEGAFSIWYAWMRSAGLLV 467
+ ++ F +Q+T T +L + F+ LV+D A P G F+ R G+ V
Sbjct: 407 YELLDEVIDFGYVQTTSTELLKSYVFNEPLVIDAARLPPLGPAAIFT--QGTKRMPGIAV 580
Query: 468 GFTVGSVSPG 477
+V + PG
Sbjct: 581 TKSVVATEPG 610
>TC225830 similar to GB|AAL49942.1|17979251|AY070476 AT3g53990/F5K20_290
{Arabidopsis thaliana;} , partial (98%)
Length = 878
Score = 28.9 bits (63), Expect = 6.0
Identities = 21/78 (26%), Positives = 37/78 (46%)
Frame = -1
Query: 330 VFISSFITMTIFTGGVVFIVGQLCIKPVHLLFFWLTYFLFPVFSLSLLQPLLRVIKMNSV 389
+ +S +++TI TG +++ +L P +L W+ +L+PLL + +S
Sbjct: 599 LLVSLLVSLTIVTGQEAWVITKLLTLPNIIL--WI-----------VLRPLLPITSESSF 459
Query: 390 KMQIVGFLFSLISSGFGF 407
K I FSL S + F
Sbjct: 458 KSSIASKSFSLASPQYIF 405
>BQ629867
Length = 429
Score = 28.5 bits (62), Expect = 7.8
Identities = 13/23 (56%), Positives = 15/23 (64%)
Frame = +3
Query: 354 IKPVHLLFFWLTYFLFPVFSLSL 376
I P ++FFWL F F FSLSL
Sbjct: 126 IYPFSIIFFWLLTFRFVGFSLSL 194
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.140 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,933,878
Number of Sequences: 63676
Number of extensions: 428181
Number of successful extensions: 3785
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 3655
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3779
length of query: 535
length of database: 12,639,632
effective HSP length: 102
effective length of query: 433
effective length of database: 6,144,680
effective search space: 2660646440
effective search space used: 2660646440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0020.10