BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0017.9
(387 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227725 369 e-102
TC227724 221 3e-60
TC224538 194 7e-50
TC224302 59 4e-09
TC216654 44 1e-04
TC216655 43 2e-04
TC219402 41 0.001
TC211724 similar to UP|Q8LLE5 (Q8LLE5) Coiled-coil protein (Frag... 41 0.001
BE022459 similar to PIR|C86255|C862 protein F12F1.11 [imported] ... 38 0.007
CA935575 37 0.012
TC228611 weakly similar to GB|CAA72487.1|1890317|ATP26A peroxida... 36 0.034
TC218535 homologue to UP|O65501 (O65501) NAD+ dependent isocitra... 35 0.044
TC206766 similar to PIR|T47576|T47576 FKBP12 interacting protein... 35 0.044
TC228358 weakly similar to GB|AAH51484.1|30185799|BC051484 GCIP-... 35 0.075
AW706428 weakly similar to GP|11994643|dbj UTP-glucose glucosylt... 35 0.075
TC220779 similar to UP|Q6T285 (Q6T285) Predicted protein, partia... 34 0.098
BE657246 similar to GP|6503306|gb| ESTs gb|AI993522 gb|T88335 a... 34 0.098
AW755534 34 0.098
TC229353 similar to GB|AAP81806.1|32441262|BT009688 At5g46840 {A... 33 0.22
TC220737 weakly similar to UP|Q9FFU0 (Q9FFU0) Similarity to poly... 33 0.22
>TC227725
Length = 1536
Score = 369 bits (947), Expect = e-102
Identities = 214/374 (57%), Positives = 267/374 (71%)
Frame = +2
Query: 14 CPHASNPYHQCTQACSKKTSHRTKPHAKNTSSSGAAPPPSNFDRRKKPGSRPEPPVVAGA 73
CP ASNPYH C + C K S T S NFDRRKK GS+PE PV+ +
Sbjct: 395 CPKASNPYHVCDEYCQKADS--------GTMSL-------NFDRRKKVGSKPELPVL-DS 526
Query: 74 VPAAKVEAIQKSNASSPISNHSEIKKVESRNDDHNHSVPVSGQIHVPLPVPDVKHVHEED 133
VP +K+ AI S+ASSP+SN+SE K ES++ N +PVSG+IHV DV + +
Sbjct: 527 VPPSKIGAIYLSDASSPLSNYSEKTKEESKS---NEIIPVSGEIHVQ----DVMPTNHKV 685
Query: 134 QQKNGVEHLANPIQTKQEDKTASDVHPGEGLATTFKGGSIDFSFSGIIPQGNEDNDDKGD 193
Q K+ + A+P K T+ D G T GGS++F FSG+ NED+D + +
Sbjct: 686 QAKHNGDKNASP---KVVPITSVD---DTGCLTKPDGGSMNFCFSGL--HDNEDSDGE-E 838
Query: 194 TESVVSESRVPVGKYHVKESFASILESILDKYGDIGASCHLESLVLRSYYIECVCFVVQE 253
TESVVSESRVPVGKYHVKESFA IL SI +KYGDIGASCHLES+V+RSYY+ECVCFVVQE
Sbjct: 839 TESVVSESRVPVGKYHVKESFAPILRSIFEKYGDIGASCHLESVVMRSYYVECVCFVVQE 1018
Query: 254 LQSDSIMHLTKSKINELVAILKDVESAQLRVAWLRSALDEIAENVEVINQHQEVDAAKAN 313
LQS IM L KSKI EL+AILKDVESAQLRVAWLR+ +DEI EN+E+I++H + AKAN
Sbjct: 1019LQSTPIMQLAKSKIKELMAILKDVESAQLRVAWLRNIVDEITENIELIDEHCMAEMAKAN 1198
Query: 314 TDREMESLREQLESEMESLAEKEQEVADIKKRIPEISDRLKELELKSAELEQSMLSSKSE 373
+DREME+L ++LES +ESLA+KEQEV DIK RI EI + L ELELKS++L +++LS KS+
Sbjct: 1199SDREMETLNKELESNLESLAQKEQEVTDIKTRIEEIREHLSELELKSSDLAKNILSIKSK 1378
Query: 374 VENLHRKSLIDELL 387
V+NL KSL+DEL+
Sbjct: 1379VDNLDSKSLLDELV 1420
>TC227724
Length = 799
Score = 221 bits (562), Expect(2) = 3e-60
Identities = 111/166 (66%), Positives = 137/166 (81%)
Frame = +2
Query: 222 LDKYGDIGASCHLESLVLRSYYIECVCFVVQELQSDSIMHLTKSKINELVAILKDVESAQ 281
L+KY DIGASCH V+RSYY+ECVCFVVQELQS IM LTKS I EL+AILKDVESAQ
Sbjct: 68 LEKYXDIGASCHWNQFVMRSYYVECVCFVVQELQSTPIMQLTKSXIKELMAILKDVESAQ 247
Query: 282 LRVAWLRSALDEIAENVEVINQHQEVDAAKANTDREMESLREQLESEMESLAEKEQEVAD 341
LRVAWLRS +DEI EN+E+I++H + AKAN+DRE+ESL ++LES +E LA+KEQEV D
Sbjct: 248 LRVAWLRSIVDEITENIELIDEHCVAETAKANSDREVESLNKELESNLEILAQKEQEVTD 427
Query: 342 IKKRIPEISDRLKELELKSAELEQSMLSSKSEVENLHRKSLIDELL 387
IK RI I +RL ELELKS +L++++LS KS+V+NL KSL+DEL+
Sbjct: 428 IKTRIEAIRERLSELELKSCDLDKNILSIKSKVDNLDSKSLLDELV 565
Score = 29.3 bits (64), Expect(2) = 3e-60
Identities = 15/23 (65%), Positives = 15/23 (65%)
Frame = +3
Query: 200 ESRVPVGKYHVKESFASILESIL 222
ESRVPV HVK A IL SIL
Sbjct: 3 ESRVPVXXXHVKXXXAPILRSIL 71
>TC224538
Length = 736
Score = 194 bits (492), Expect = 7e-50
Identities = 121/260 (46%), Positives = 152/260 (57%), Gaps = 7/260 (2%)
Frame = +1
Query: 12 PNCPHASNPYHQCTQACSKKTSHRTKPHAKNTSSSGAAPPPSNFDRRKKPGSRPEPPVVA 71
P CP ASNPYHQC C K+ S APPP D++KK GS+PEPPV+
Sbjct: 70 PGCPKASNPYHQCDHNCYKRLS------------DSGAPPPIKLDKKKKLGSKPEPPVL- 210
Query: 72 GAVPAAKVEAIQKSNASSPISNHSEIKKVESRNDDH----NHSVPVSGQIHVPLPVPDVK 127
+VPA+KV AI S+ASSPIS +SE KKVE+ N++H + V +HV L P
Sbjct: 211 DSVPASKVGAIYLSDASSPISQYSEKKKVETENNEHVSPQTNIQAVKICMHVFLSQP--- 381
Query: 128 HVHEEDQQ-KNGVEHLANPIQTKQ-EDKTASDVHPGEGLATTFKGGSIDFSFSGIIPQGN 185
V+ +DQ K G +H+ NP+ T EDK AS+ I+P
Sbjct: 382 -VNNKDQPIKEGEQHVGNPMLTNDDEDKIASNK---------------------IVPISL 495
Query: 186 EDNDDKGDTESVVSESRVPVGKYHVKESFASILESILDKYGDIGASCHLESLVLRSYYIE 245
++ D + SE R+ VG+Y +KESF SIL+SILDKYGDIG SC LES+ +RSYYIE
Sbjct: 496 PISESDDDESVMSSEGRLQVGRYQIKESFGSILQSILDKYGDIGESCQLESVAMRSYYIE 675
Query: 246 CVCFVVQEL-QSDSIMHLTK 264
CVCFVVQEL S SIM L+K
Sbjct: 676 CVCFVVQELHNSSSIMQLSK 735
>TC224302
Length = 442
Score = 58.9 bits (141), Expect = 4e-09
Identities = 30/43 (69%), Positives = 35/43 (80%)
Frame = +2
Query: 345 RIPEISDRLKELELKSAELEQSMLSSKSEVENLHRKSLIDELL 387
RIPEI RL +L+LKS EL +SMLS KS+V NLH KSL+DELL
Sbjct: 5 RIPEIRGRLSQLQLKSEELNRSMLSIKSKVHNLHIKSLVDELL 133
>TC216654
Length = 1170
Score = 43.9 bits (102), Expect = 1e-04
Identities = 37/124 (29%), Positives = 66/124 (52%), Gaps = 3/124 (2%)
Frame = +1
Query: 250 VVQELQSDSIMHLTKSKINELVAILKDVESAQLRVAWLRSALDEIAENVEVINQHQEVDA 309
+++EL DS+ + KSK +++ +LK VE L + + E ++ + QE+
Sbjct: 424 IIKELVMDSVDSI-KSK-GDVITVLK-VEKEDLESSLYK-------EKLQTLQLKQELSE 573
Query: 310 AKA-NTD--REMESLREQLESEMESLAEKEQEVADIKKRIPEISDRLKELELKSAELEQS 366
K N+D +E++S+R QL SE + E EVA++ +++ I KELEL + S
Sbjct: 574 TKTRNSDLCKELQSIRGQLASEQSRCFKLEVEVAELSQKLQTIGTLQKELELLQRQKAAS 753
Query: 367 MLSS 370
L++
Sbjct: 754 ELAA 765
>TC216655
Length = 454
Score = 43.1 bits (100), Expect = 2e-04
Identities = 34/105 (32%), Positives = 57/105 (53%), Gaps = 3/105 (2%)
Frame = +1
Query: 257 DSIMHLTKSKINELVAILKDVESAQLRVAWLRSALDEIAENVEVINQHQEVDAAKA-NTD 315
DSI+ + KSK +++ +LK VE L + + E ++ + QE+ K N+D
Sbjct: 160 DSIVCIIKSK-GDVITVLK-VEKEDLESSLYK-------EKLQTLQLKQELSETKTRNSD 312
Query: 316 --REMESLREQLESEMESLAEKEQEVADIKKRIPEISDRLKELEL 358
+E++S+R QL SE + E EVA++ +++ I KELEL
Sbjct: 313 LCKELQSIRGQLASEQSRCFKLEVEVAELSQKLQTIGTLQKELEL 447
>TC219402
Length = 674
Score = 40.8 bits (94), Expect = 0.001
Identities = 35/125 (28%), Positives = 65/125 (52%), Gaps = 3/125 (2%)
Frame = +3
Query: 250 VVQELQSDSIMHLTKSKINELVAILKDVESAQLRVAWLRSALDEIAENVEVINQHQEVDA 309
V E ++ + KSK ++V++LK E +L S + E +++ E ++
Sbjct: 183 VESEFEAGHDSNPPKSK-GDIVSVLK-AEKEELE----SSLIKEKHHALQLKQDLAEAES 344
Query: 310 AKANTDREMESLREQLESEMESLAEKEQEVADIKKRIPEISDRLKELEL---KSAELEQS 366
++ +E++S+R QL SE + E EVA++ +++ I KELEL + A EQ+
Sbjct: 345 CNSDLYKELQSVRGQLASEQSRCFKLEVEVAELGQKLQTIGTLQKELELLQRQKAASEQA 524
Query: 367 MLSSK 371
L++K
Sbjct: 525 ALNAK 539
>TC211724 similar to UP|Q8LLE5 (Q8LLE5) Coiled-coil protein (Fragment),
partial (12%)
Length = 1086
Score = 40.8 bits (94), Expect = 0.001
Identities = 28/117 (23%), Positives = 60/117 (50%), Gaps = 8/117 (6%)
Frame = +3
Query: 269 ELVAILKDVESAQLRVAWLRSALDE-IAENVEVINQHQEVDAAKANTDREMESLREQLES 327
E+ A+++ + + ++ + + ++ I +NVE+ + ++++A K D + + QLE+
Sbjct: 162 EIEAMIQKDDELEKKLGKMEAEMEAMIQKNVELEKKLKKMEAGKVEVDMVLTKYQMQLET 341
Query: 328 EMESLAEKEQEVADI-------KKRIPEISDRLKELELKSAELEQSMLSSKSEVENL 377
E E +VA+ KK E + LK + K A +E ++ +++EVE L
Sbjct: 342 SESQTREAELKVAEFQTQLALAKKSNQEACEELKATKAKKAIVESTLKLTQTEVEEL 512
>BE022459 similar to PIR|C86255|C862 protein F12F1.11 [imported] -
Arabidopsis thaliana, partial (41%)
Length = 411
Score = 38.1 bits (87), Expect = 0.007
Identities = 21/60 (35%), Positives = 33/60 (55%)
Frame = +2
Query: 18 SNPYHQCTQACSKKTSHRTKPHAKNTSSSGAAPPPSNFDRRKKPGSRPEPPVVAGAVPAA 77
++PY + + S++ SHR + TSS A PPPS R +KP +P P+ + A +A
Sbjct: 95 NSPYSRRPSSPSRRRSHRRTEKSSPTSSP-APPPPSPATRERKPPPKPTIPLHSAAAASA 271
>CA935575
Length = 410
Score = 37.4 bits (85), Expect = 0.012
Identities = 33/134 (24%), Positives = 69/134 (50%), Gaps = 8/134 (5%)
Frame = +1
Query: 248 CFVVQELQSDSIMHLTKSKINELVAILKDVE----SAQLRVAWLRSALDEIAENVEVINQ 303
C + E ++ S+ ++IN L+ +LK+ E + + L+ L E+ EVI+
Sbjct: 22 CLKLTEEENSSL----GNEINRLIRLLKETEEEANAKREEEGQLKENLKEV--EAEVIHL 183
Query: 304 HQEVDAAKANTDREMESLREQLESEMESLAEKEQEV----ADIKKRIPEISDRLKELELK 359
+E+ AKA + + ESL ++ E+E +++ E+ +E+ + K++ E+S L E+ +
Sbjct: 184 QEELKEAKAESMKLKESLLDK-ENEFQNIFEENEELRLRESTSIKKVEELSKMLDEVTSR 360
Query: 360 SAELEQSMLSSKSE 373
+ E L+ +
Sbjct: 361 NQTEENGDLTDSEK 402
>TC228611 weakly similar to GB|CAA72487.1|1890317|ATP26A peroxidase ATP26a
{Arabidopsis thaliana;} , partial (49%)
Length = 731
Score = 35.8 bits (81), Expect = 0.034
Identities = 26/89 (29%), Positives = 40/89 (44%), Gaps = 2/89 (2%)
Frame = +3
Query: 3 AASNGTASNPNC--PHASNPYHQCTQACSKKTSHRTKPHAKNTSSSGAAPPPSNFDRRKK 60
A+S+ TAS+P P +S+P + TS P +TSSS PP S+
Sbjct: 198 ASSSTTASSPTAVTPPSSSPPRPSPGPSATPTSTSPSPATPSTSSSAPRPPLSSPAPTPS 377
Query: 61 PGSRPEPPVVAGAVPAAKVEAIQKSNASS 89
P PP A + P + + S+A++
Sbjct: 378 PAPTFSPPPPATSSPCSAAPSSPSSSAAA 464
>TC218535 homologue to UP|O65501 (O65501) NAD+ dependent isocitrate
dehydrogenase subunit 1 (AT4g35260/F23E12_180) , partial
(94%)
Length = 1331
Score = 35.4 bits (80), Expect = 0.044
Identities = 28/89 (31%), Positives = 44/89 (48%), Gaps = 8/89 (8%)
Frame = -2
Query: 9 ASNPNCPHASNPYHQCTQACSKKTS------HRTKPHAKNTSSS--GAAPPPSNFDRRKK 60
+S+P+ P +SN H + CS ++ H + P A TS S G A PPS+ +
Sbjct: 337 SSHPHPPESSN--HPSSTPCSSVSNPAPPPAHASCPRAHRTSRSIWGRASPPSHAPPHES 164
Query: 61 PGSRPEPPVVAGAVPAAKVEAIQKSNASS 89
P ++ PPV A + PAA ++ + S
Sbjct: 163 PEAQFRPPVSA-SPPAAHRPPVEACTSRS 80
>TC206766 similar to PIR|T47576|T47576 FKBP12 interacting protein (FIP37) -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(74%)
Length = 1952
Score = 35.4 bits (80), Expect = 0.044
Identities = 35/126 (27%), Positives = 61/126 (47%), Gaps = 8/126 (6%)
Frame = +3
Query: 268 NELVAILKDVESAQLRVAWLRSALDEIA-ENVEVINQHQEVDAAKANTDREMESLREQLE 326
NEL A +++S + S L I E +IN Q + +++ ESLREQLE
Sbjct: 423 NELEAAKSEIQSWHSTLKNQPSILAGITPEPKMLINYLQALKSSE-------ESLREQLE 581
Query: 327 SEME-------SLAEKEQEVADIKKRIPEISDRLKELELKSAELEQSMLSSKSEVENLHR 379
+ + A++EQE+A++K + ++ +LK ++S L +L E
Sbjct: 582 KAKKKEAAFIVTFAKREQEIAELKSAVRDLKVQLKPPSMQSRRL---LLDPAVHEEFTRL 752
Query: 380 KSLIDE 385
K+L++E
Sbjct: 753 KNLVEE 770
>TC228358 weakly similar to GB|AAH51484.1|30185799|BC051484 GCIP-interacting
protein p29 {Mus musculus;} , partial (28%)
Length = 1460
Score = 34.7 bits (78), Expect = 0.075
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +2
Query: 5 SNGTASNPNCPHASNPYHQCTQACSKKTSHRTKPHAKNTSSSGAAPPPSN 54
S+ A +P+C +ASNPYH+C+ C + + + S G A +N
Sbjct: 131 SDERAVHPDCRNASNPYHECSDYCFRVIAEAKIRSQQQESEVGQASGGNN 280
>AW706428 weakly similar to GP|11994643|dbj UTP-glucose glucosyltransferase
{Arabidopsis thaliana}, partial (7%)
Length = 331
Score = 34.7 bits (78), Expect = 0.075
Identities = 28/95 (29%), Positives = 41/95 (42%), Gaps = 5/95 (5%)
Frame = -3
Query: 15 PHASNPYHQCTQACSKKTSHRTKPHAKNTSSSGAAPPPSNFDRRKKPGSRPEPPVVAGAV 74
PH P H T+ ++ + PH++ SSS PP P S PP +
Sbjct: 266 PHTLLP-HSSTKQLRLQSHPVSPPHSQTRSSSSILSPP------PAPQSSTHPPPASS*K 108
Query: 75 PAAKVEAIQKSNASS-----PISNHSEIKKVESRN 104
A + E + S SS P SNHSE ++S++
Sbjct: 107 NAPQTEVMLPSATSSSRFPPPCSNHSE*PTLQSQD 3
>TC220779 similar to UP|Q6T285 (Q6T285) Predicted protein, partial (71%)
Length = 769
Score = 34.3 bits (77), Expect = 0.098
Identities = 21/71 (29%), Positives = 35/71 (48%), Gaps = 1/71 (1%)
Frame = +3
Query: 276 DVESAQLRVAWLRSALDEIAENVEVINQHQEVDAAKANT-DREMESLREQLESEMESLAE 334
D ESA WL ++ NV+V+ + + + N DRE++ L EQLE + L
Sbjct: 402 DFESANYPKGWLIGKKRKLV-NVDVVESMRRIAIQEMNRKDREIDGLNEQLEEDSRCLEH 578
Query: 335 KEQEVADIKKR 345
+ ++ D K +
Sbjct: 579 LQLQLVDEKSK 611
>BE657246 similar to GP|6503306|gb| ESTs gb|AI993522 gb|T88335 and
gb|AA540932 come from this gene. {Arabidopsis thaliana},
partial (58%)
Length = 653
Score = 34.3 bits (77), Expect = 0.098
Identities = 21/71 (29%), Positives = 35/71 (48%), Gaps = 1/71 (1%)
Frame = -1
Query: 276 DVESAQLRVAWLRSALDEIAENVEVINQHQEVDAAKANT-DREMESLREQLESEMESLAE 334
D ESA WL ++ NV+V+ + + + N DRE++ L EQLE + L
Sbjct: 476 DFESANYPKGWLIGKKRKLV-NVDVVESMRRIAIQEMNRKDREIDGLNEQLEEDSRCLEH 300
Query: 335 KEQEVADIKKR 345
+ ++ D K +
Sbjct: 299 LQLQLVDEKSK 267
>AW755534
Length = 434
Score = 34.3 bits (77), Expect = 0.098
Identities = 29/96 (30%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +2
Query: 10 SNPNCPHASNPY--HQCTQACSKKTSHRTKPHAKNTSSSGAAPPPSNFDRRKKPGSRPEP 67
+NPN S P H T + S T P +S S + PP++ + +PGS P
Sbjct: 125 ANPN----SKPLLLHPPTATTTPSPSGSTSPSPSPSSLSSLS*PPASPPKTPRPGSSPSL 292
Query: 68 PVVAGAVPAAKVEAIQKSNASSPISNHSEIKKVESR 103
P A P A + K + P S S K+V R
Sbjct: 293 PPSATTTPTA-APSRSKHIPTKPPSKSSPSKRVPHR 397
>TC229353 similar to GB|AAP81806.1|32441262|BT009688 At5g46840 {Arabidopsis
thaliana;} , partial (46%)
Length = 1624
Score = 33.1 bits (74), Expect = 0.22
Identities = 17/51 (33%), Positives = 23/51 (44%)
Frame = +3
Query: 39 HAKNTSSSGAAPPPSNFDRRKKPGSRPEPPVVAGAVPAAKVEAIQKSNASS 89
HAK ++ P PS+ P RP P A + PA K +S +SS
Sbjct: 813 HAKADATPSKRPNPSSAHAPSTPAKRPNPSAHAPSTPAKKFSVASRSPSSS 965
>TC220737 weakly similar to UP|Q9FFU0 (Q9FFU0) Similarity to
polyadenylate-binding protein 5, partial (33%)
Length = 450
Score = 33.1 bits (74), Expect = 0.22
Identities = 25/85 (29%), Positives = 34/85 (39%), Gaps = 4/85 (4%)
Frame = +2
Query: 10 SNPNCPHASNPYHQCTQACSKKTSHRTKPHAKNTSSSGAAPPPSNFDRRKKPGSRPE--- 66
+ P AS P + + + S TS R P A +SSS P F R P +RP
Sbjct: 47 TRPT*ATASPPPARWSPSKSSATSSRASPRATASSSSSPTPQRKGFSRPTTPPTRPSGSI 226
Query: 67 -PPVVAGAVPAAKVEAIQKSNASSP 90
PP + + S+A SP
Sbjct: 227 GPPSASASAGPMPPWNTPSSSAISP 301
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.308 0.126 0.345
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,934,085
Number of Sequences: 63676
Number of extensions: 239108
Number of successful extensions: 2080
Number of sequences better than 10.0: 148
Number of HSP's better than 10.0 without gapping: 1941
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2037
length of query: 387
length of database: 12,639,632
effective HSP length: 99
effective length of query: 288
effective length of database: 6,335,708
effective search space: 1824683904
effective search space used: 1824683904
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0017.9