Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0012b.5
         (815 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC229857 similar to UP|Q94544 (Q94544) Phosphatidylinositol 4-ph...   643  0.0
TC220682 similar to UP|Q94544 (Q94544) Phosphatidylinositol 4-ph...   325  5e-89
TC222035 similar to UP|O04095 (O04095) Phosphatidylinositol-4-ph...   308  5e-84
BE805831                                                              241  7e-64
TC230442 similar to UP|O04095 (O04095) Phosphatidylinositol-4-ph...   237  2e-62
BQ613037                                                              191  9e-49
TC234608 similar to UP|Q70Z13 (Q70Z13) Phosphatidylinositol-4-ph...   158  1e-38
BI788236                                                              122  8e-28
TC233098 similar to UP|Q9M1K2 (Q9M1K2) Phosphatidylinositol-4-ph...    84  3e-16
TC221048                                                               82  1e-15
TC229966 similar to GB|AAF16539.1|6552740|AC013482 T26F17.15 {Ar...    81  2e-15
AW278716 weakly similar to GP|22775626|db 1-phosphatidylinositol...    77  4e-14
TC212252                                                               50  1e-11
TC217753 weakly similar to UP|FAB1_YEAST (P34756) 1-phosphatidyl...    67  2e-11
TC213097                                                               67  4e-11
TC205219 similar to GB|AAF16539.1|6552740|AC013482 T26F17.15 {Ar...    54  6e-11
TC218384 similar to GB|AAQ56792.1|34098819|BT010349 At1g71010 {A...    65  2e-10
TC227744 similar to UP|Q9LUM0 (Q9LUM0) Emb|CAB36798.1, partial (...    64  2e-10
TC227161 similar to UP|Q9LUM0 (Q9LUM0) Emb|CAB36798.1, partial (...    60  4e-09
BM886461                                                               55  2e-07

>TC229857 similar to UP|Q94544 (Q94544) Phosphatidylinositol 4-phosphate
           5-kinase , partial (6%)
          Length = 1296

 Score =  643 bits (1659), Expect = 0.0
 Identities = 321/388 (82%), Positives = 350/388 (89%)
 Frame = +3

Query: 424 KASFWMNFPREGSPLTPSHQSEDFKWKDYCPMVFRNLRELFKIDAADYMISICGNDALRE 483
           +ASFWMNFP+EGS LTP HQSE FKWKDYCPMVFRNLRELFKIDAADYM+SICGNDALRE
Sbjct: 3   RASFWMNFPKEGSQLTPPHQSEGFKWKDYCPMVFRNLRELFKIDAADYMMSICGNDALRE 182

Query: 484 LSSPGKSGSVFFLSQDDRFMIKTLRKSELKIFLRMLPDYHHHVKSYENTLITKFFGLHRI 543
           LSSPGKSGSVFFLSQDDRFMIKTLR+SE+K+ LRMLPDYHHHVK+Y+NTLITKFFGLHRI
Sbjct: 183 LSSPGKSGSVFFLSQDDRFMIKTLRRSEVKVLLRMLPDYHHHVKTYDNTLITKFFGLHRI 362

Query: 544 KPSSGQKFRFAVMGNMFCTELRIHRRFDLKGSSFGRSSDKIEIDENTTLKDLDLNYSFFL 603
            PSSGQKFRF VMGNMFCTELRIHRR+DLKGSS GRSSDKIEIDENTTLKDLDLNY F+L
Sbjct: 363 IPSSGQKFRFVVMGNMFCTELRIHRRYDLKGSSLGRSSDKIEIDENTTLKDLDLNYCFYL 542

Query: 604 EPSWRDSLLNQIKIDSKFLEAQHIMDYSLLLGVHYRAPQHVRPLMSYNQRSISTDGLAVL 663
           EPSW++SLL QI+IDSKFLE Q IMDYSLLLGVHYRAPQ + P   YNQ   + DGL +L
Sbjct: 543 EPSWQESLLKQIEIDSKFLELQQIMDYSLLLGVHYRAPQQLHP---YNQNR-NADGLPIL 710

Query: 664 AEEDPLEDEVFNYPQGLVLVPRGAGDNSVVVGSHMRGGRLRASSAGDEVVDLLLPGTARL 723
           AEEDPLEDE  NYPQGLVLVPRG  D+SVVVGSH++G +LRA  A DE VDLLLPGTARL
Sbjct: 711 AEEDPLEDEGSNYPQGLVLVPRGTDDDSVVVGSHIKGSQLRA--AVDEEVDLLLPGTARL 884

Query: 724 QIQLGVNMPSRAEQNQIIGKQEKQMFHEVNDVVLYLGIIDILQDYNMTKKLEHAYKSLQF 783
           QIQLGVNMP+RAE  QI GK+E QMFHE  DVVLYLGIIDILQ+YNMTKK+EHAYKS+QF
Sbjct: 885 QIQLGVNMPARAE--QIPGKEEVQMFHEAYDVVLYLGIIDILQEYNMTKKIEHAYKSIQF 1058

Query: 784 DSVSISVVDPAFYSRRFLEFIQKVFPPN 811
           DS+SIS VDP FYSRRFL+FIQK+FPP+
Sbjct: 1059DSLSISAVDPTFYSRRFLDFIQKMFPPH 1142


>TC220682 similar to UP|Q94544 (Q94544) Phosphatidylinositol 4-phosphate
           5-kinase , partial (7%)
          Length = 1280

 Score =  325 bits (833), Expect = 5e-89
 Identities = 182/355 (51%), Positives = 234/355 (65%), Gaps = 4/355 (1%)
 Frame = +1

Query: 458 RNLRELFKIDAADYMISICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSELKIFLR 517
           R LR+LF++D ADYM++ICG+DALRELSSPGKSGS F+L+QDDRFMIKT++KSE+K+ LR
Sbjct: 1   RQLRKLFQVDPADYMLAICGHDALRELSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLLR 180

Query: 518 MLPDYHHHVKSYENTLITKFFGLHRIKPSSGQKFRFAVMGNMFCTELRIHRRFDLKGSSF 577
           ML  Y+ HV  YEN+L+TKF+G+H +KP  GQK RF VMGN+FC+E +IHRRFDLKGSS 
Sbjct: 181 MLRSYYQHVSKYENSLVTKFYGVHCVKPIGGQKTRFIVMGNLFCSEYQIHRRFDLKGSSH 360

Query: 578 GRSSDKI-EIDENTTLKDLDLNYSFFLEPSWRDSLLNQIKIDSKFLEAQHIMDYSLLLGV 636
           GR++DK  EIDE TTLKDLDLN+ F L+ +W    + QI+ D +FLEA+ IMDYSLL+G+
Sbjct: 361 GRTTDKTKEIDETTTLKDLDLNFVFRLQNNWFQDFIKQIERDCEFLEAEGIMDYSLLVGL 540

Query: 637 HYRAPQHVRPLMSYNQRSISTDGLAVLAEEDPLEDEVFNYPQGLVLVPRGAGDNSVVVGS 696
           H+R         +Y +  +S   L    + D  + E F                      
Sbjct: 541 HFRDDN------TYEKMGLSPFLLRT-GKWDSYQSEKF---------------------- 633

Query: 697 HMRGGRLRASSAGDEVVDLLLPGTARLQIQLGVNMPSRAEQ---NQIIGKQEKQMFHEVN 753
            MRG R   +   D   D +  G   L I+LG NMP+RAE+        +       E  
Sbjct: 634 -MRGYRFLEAELQDR--DRVKSGRKSL-IRLGANMPARAERMARRSDFDQYTPCCSGETY 801

Query: 754 DVVLYLGIIDILQDYNMTKKLEHAYKSLQFDSVSISVVDPAFYSRRFLEFIQKVF 808
           DVVLY GIIDILQDY+++KKLEHAYKSLQ D  SIS VDP  YS+RF +F+ ++F
Sbjct: 802 DVVLYCGIIDILQDYDISKKLEHAYKSLQVDPSSISAVDPKLYSKRFRDFVGRIF 966


>TC222035 similar to UP|O04095 (O04095) Phosphatidylinositol-4-phosphate
           5-kinase isolog; 89655-95590, partial (31%)
          Length = 908

 Score =  308 bits (790), Expect = 5e-84
 Identities = 166/306 (54%), Positives = 213/306 (69%), Gaps = 10/306 (3%)
 Frame = +3

Query: 514 IFLRMLPDYHHHVKSYENTLITKFFGLHRIKPSSGQKFRFAVMGNMFCTELRIHRRFDLK 573
           + L MLP Y+HHV SYENTLITKFFGLHRI    G+K RF VMGNMFCTEL IHRR+DLK
Sbjct: 3   VMLNMLPKYYHHVGSYENTLITKFFGLHRITLRGGKKVRFVVMGNMFCTELHIHRRYDLK 182

Query: 574 GSSFGRSSDKIEIDENTTLKDLDLNYSFFLEPSWRDSLLNQIKIDSKFLEAQHIMDYSLL 633
           GS+ GR + + +I+ NTTLKDLDL Y F ++   R+SL  QI +D  FLE+QHI+DYSLL
Sbjct: 183 GSTQGRYTKEDKINSNTTLKDLDLKYEFHMDKKLRESLFKQISLDCMFLESQHIIDYSLL 362

Query: 634 LGVHYRAPQHVRPLMSYNQRSISTDGLAVLAEEDPLEDEVFN------YPQGLVLVPRGA 687
           LG+H+RAP++++ L       +    L  L    P ED+          P+GL+LV    
Sbjct: 363 LGLHFRAPENLKAL-------VEPPRLMQLPHSLPSEDDAHKQGEQLIIPKGLLLVAHEP 521

Query: 688 GDNSVVVGSHMRGGRLRASSAGDEVVDLLLPGTARLQIQLGVNMPSRAE---QNQIIGKQ 744
              +   G H+RG  LRA S GD+ VDLLLPGTARL++QLGVNMP++A    Q   + + 
Sbjct: 522 SFVNTAPGPHIRGNTLRAYSIGDKQVDLLLPGTARLRVQLGVNMPAQATRKLQGDKVEES 701

Query: 745 EKQMFHEVNDVVLYLGIIDILQDYNMTKKLEHAYKSLQFDSVSISVVDPAFYSRRFLEFI 804
           E ++F EV DVVLY+GIIDILQ+Y + KKLEHA KSLQ+D ++ISVV+P  Y+ RF+ F+
Sbjct: 702 EVELF-EVYDVVLYMGIIDILQEYTVKKKLEHACKSLQYDPMTISVVEPKTYAERFINFM 878

Query: 805 Q-KVFP 809
           + KVFP
Sbjct: 879 EKKVFP 896


>BE805831 
          Length = 375

 Score =  241 bits (616), Expect = 7e-64
 Identities = 111/123 (90%), Positives = 119/123 (96%)
 Frame = +2

Query: 420 DFGSKASFWMNFPREGSPLTPSHQSEDFKWKDYCPMVFRNLRELFKIDAADYMISICGND 479
           DFG KASFWM+FP+EGS LTP+HQS+DFKWKDYCPMVFRNLRELFKIDAADYM+SICGND
Sbjct: 2   DFGPKASFWMDFPKEGSQLTPTHQSDDFKWKDYCPMVFRNLRELFKIDAADYMMSICGND 181

Query: 480 ALRELSSPGKSGSVFFLSQDDRFMIKTLRKSELKIFLRMLPDYHHHVKSYENTLITKFFG 539
            LRELSSPGKSGSVFFLSQDDRFMIKTLR+SE+K+ LRMLPDYHHHVKSYENTLITKFFG
Sbjct: 182 TLRELSSPGKSGSVFFLSQDDRFMIKTLRRSEVKVLLRMLPDYHHHVKSYENTLITKFFG 361

Query: 540 LHR 542
           LHR
Sbjct: 362 LHR 370


>TC230442 similar to UP|O04095 (O04095) Phosphatidylinositol-4-phosphate
           5-kinase isolog; 89655-95590, partial (24%)
          Length = 707

 Score =  237 bits (604), Expect = 2e-62
 Identities = 127/241 (52%), Positives = 170/241 (69%), Gaps = 5/241 (2%)
 Frame = +1

Query: 548 GQKFRFAVMGNMFCTELRIHRRFDLKGSSFGRSSDKIEIDENTTLKDLDLNYSFFLEPSW 607
           G+K RF VMGN+FCTEL+IHRR+DLKGS  GR ++  +I+ NTTLKDLDL Y F ++   
Sbjct: 1   GKKVRFVVMGNVFCTELQIHRRYDLKGSKQGRYTNNDKINCNTTLKDLDLKYEFHMDKKL 180

Query: 608 RDSLLNQIKIDSKFLEAQHIMDYSLLLGVHYRAPQHVRPLMSYNQRSISTDGLAVLAEED 667
           R+SLL QI +D KFLE+QHI+DYSLLLG+H+RAP++++  + +++   S      L   D
Sbjct: 181 RESLLKQISLDCKFLESQHIIDYSLLLGLHFRAPENMKAFVEHHE---SVQRQESLPSGD 351

Query: 668 PLEDEVFNYPQGLVLVPRGAGDNSVVVGSHMRGGRLRASSAGDEVVDLLLPGTARLQIQL 727
             E ++   P+GL+LV       +   G H+RG  LRA S GD  VDLLLPG ARL++QL
Sbjct: 352 --EGQLLILPKGLLLVSHEPSIVNTAPGPHIRGNTLRAYSMGDNEVDLLLPGIARLRVQL 525

Query: 728 GVNMPSRA-----EQNQIIGKQEKQMFHEVNDVVLYLGIIDILQDYNMTKKLEHAYKSLQ 782
           GVNMP++A     E    +  +E ++F EV DVVLY+G+IDILQ+YN+ KK+EHAYKSLQ
Sbjct: 526 GVNMPAQATRRLQEDKDKVEAKEVELF-EVYDVVLYMGVIDILQNYNLRKKIEHAYKSLQ 702

Query: 783 F 783
           F
Sbjct: 703 F 705


>BQ613037 
          Length = 456

 Score =  191 bits (486), Expect = 9e-49
 Identities = 96/119 (80%), Positives = 106/119 (88%)
 Frame = +1

Query: 697 HMRGGRLRASSAGDEVVDLLLPGTARLQIQLGVNMPSRAEQNQIIGKQEKQMFHEVNDVV 756
           H+RG RLRAS+AGDE VDLLLPGTARLQIQLGVNMP+RAEQ  I GK+E QMFHE  DVV
Sbjct: 1   HIRGSRLRASAAGDEEVDLLLPGTARLQIQLGVNMPARAEQ--IPGKEEMQMFHEAYDVV 174

Query: 757 LYLGIIDILQDYNMTKKLEHAYKSLQFDSVSISVVDPAFYSRRFLEFIQKVFPPNASTG 815
           LYLGIIDILQ+YNMTKK+EHAYKS+QFDS+SIS VDP FYSRRFL+FIQKVFPP+   G
Sbjct: 175 LYLGIIDILQEYNMTKKIEHAYKSIQFDSLSISAVDPTFYSRRFLDFIQKVFPPHEMAG 351


>TC234608 similar to UP|Q70Z13 (Q70Z13) Phosphatidylinositol-4-phosphate
           5-kinase  (Fragment) , partial (9%)
          Length = 467

 Score =  158 bits (399), Expect = 1e-38
 Identities = 79/92 (85%), Positives = 83/92 (89%)
 Frame = +2

Query: 724 QIQLGVNMPSRAEQNQIIGKQEKQMFHEVNDVVLYLGIIDILQDYNMTKKLEHAYKSLQF 783
           QIQLGVNMPSRAEQ     KQEKQMFHEV DVVLYLGIIDILQDYNMTK++EHAYKSLQF
Sbjct: 2   QIQLGVNMPSRAEQISGKQKQEKQMFHEVYDVVLYLGIIDILQDYNMTKRIEHAYKSLQF 181

Query: 784 DSVSISVVDPAFYSRRFLEFIQKVFPPNASTG 815
           DS+SIS VDP FYS RFLEFIQKVFPPNA+TG
Sbjct: 182 DSLSISAVDPTFYSHRFLEFIQKVFPPNATTG 277


>BI788236 
          Length = 425

 Score =  122 bits (305), Expect = 8e-28
 Identities = 62/103 (60%), Positives = 84/103 (81%), Gaps = 3/103 (2%)
 Frame = +1

Query: 710 DEVVDLLLPGTARLQIQLGVNMPSRAEQN--QIIGKQEKQMFHEVNDVVLYLGIIDILQD 767
           D  VDLLLPGTARL++QLGVNMP++A +   + +  +E ++F EV DVVLY+G+IDILQ+
Sbjct: 1   DNEVDLLLPGTARLRVQLGVNMPAQATRKVEEEVEAKEVELF-EVYDVVLYMGVIDILQN 177

Query: 768 YNMTKKLEHAYKSLQFDSVSISVVDPAFYSRRFLEFI-QKVFP 809
           YN+ KK+EHAYKSLQFD ++ISVV+P  Y  RF++F+ +KVFP
Sbjct: 178 YNLRKKIEHAYKSLQFDPMTISVVEPKMYDERFIKFLEEKVFP 306


>TC233098 similar to UP|Q9M1K2 (Q9M1K2) Phosphatidylinositol-4-phosphate
           5-kinase-like protein, partial (12%)
          Length = 781

 Score = 83.6 bits (205), Expect = 3e-16
 Identities = 45/92 (48%), Positives = 62/92 (66%), Gaps = 8/92 (8%)
 Frame = +1

Query: 725 IQLGVNMPSRAEQN--------QIIGKQEKQMFHEVNDVVLYLGIIDILQDYNMTKKLEH 776
           I LG+NMP++ E+         Q +G+   + ++ V    L  GIIDILQDY+++KKLEH
Sbjct: 136 IILGMNMPAKVERTIRRSGCELQFVGEPIGEFYNGV----LTFGIIDILQDYDISKKLEH 303

Query: 777 AYKSLQFDSVSISVVDPAFYSRRFLEFIQKVF 808
           AYKS+Q+D  SIS VDP  YSRRF +FI ++F
Sbjct: 304 AYKSIQYDPTSISAVDPIQYSRRFRDFIFRIF 399


>TC221048 
          Length = 528

 Score = 81.6 bits (200), Expect = 1e-15
 Identities = 34/63 (53%), Positives = 39/63 (60%)
 Frame = +1

Query: 45  ELLLSNGESYSGTLFGNTPEGHGTYVWSDRCVYEGGWRRGMRHGNGKIRWPYGAVYEGEF 104
           E  L NG+ Y G+  GN P G G Y+W D CVYEG W++G   G GK  WP GA YEGEF
Sbjct: 340 ERRLPNGDVYMGSFSGNAPSGSGKYLWRDGCVYEGEWKKGKACGKGKFSWPSGATYEGEF 519

Query: 105 SGG 107
             G
Sbjct: 520 KSG 528



 Score = 62.4 bits (150), Expect = 8e-10
 Identities = 27/59 (45%), Positives = 35/59 (58%)
 Frame = +1

Query: 141 PNGDILEGSWIQGTPEGPGKYTWTNGNVYVGNMKGGTMSGKGTLTWINGDSYEGSWLNG 199
           PNGD+  GS+    P G GKY W +G VY G  K G   GKG  +W +G +YEG + +G
Sbjct: 352 PNGDVYMGSFSGNAPSGSGKYLWRDGCVYEGEWKKGKACGKGKFSWPSGATYEGEFKSG 528



 Score = 54.3 bits (129), Expect = 2e-07
 Identities = 23/58 (39%), Positives = 30/58 (51%)
 Frame = +1

Query: 165 NGNVYVGNMKGGTMSGKGTLTWINGDSYEGSWLNGMMHGFGAYTWSGGGCYVGTWTCG 222
           NG+VY+G+  G   SG G   W +G  YEG W  G   G G ++W  G  Y G +  G
Sbjct: 355 NGDVYMGSFSGNAPSGSGKYLWRDGCVYEGEWKKGKACGKGKFSWPSGATYEGEFKSG 528



 Score = 50.4 bits (119), Expect = 3e-06
 Identities = 29/84 (34%), Positives = 37/84 (43%)
 Frame = +1

Query: 93  RWPYGAVYEGEFSGGYIQGTGKYIGSDHLTYKGRWRLNLKHGLGYQVYPNGDILEGSWIQ 152
           R P G VY G FSG    G+GKY+          WR             +G + EG W +
Sbjct: 346 RLPNGDVYMGSFSGNAPSGSGKYL----------WR-------------DGCVYEGEWKK 456

Query: 153 GTPEGPGKYTWTNGNVYVGNMKGG 176
           G   G GK++W +G  Y G  K G
Sbjct: 457 GKACGKGKFSWPSGATYEGEFKSG 528



 Score = 41.2 bits (95), Expect = 0.002
 Identities = 21/49 (42%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
 Frame = +1

Query: 188 NGDSYEGSWLNGMMHGFGAYTWSGGGCYVGTWTCGLKDGKGTF-YPKGS 235
           NGD Y GS+      G G Y W  G  Y G W  G   GKG F +P G+
Sbjct: 355 NGDVYMGSFSGNAPSGSGKYLWRDGCVYEGEWKKGKACGKGKFSWPSGA 501


>TC229966 similar to GB|AAF16539.1|6552740|AC013482 T26F17.15 {Arabidopsis
           thaliana;} , partial (44%)
          Length = 757

 Score = 80.9 bits (198), Expect = 2e-15
 Identities = 36/100 (36%), Positives = 50/100 (50%)
 Frame = +2

Query: 131 LKHGLGYQVYPNGDILEGSWIQGTPEGPGKYTWTNGNVYVGNMKGGTMSGKGTLTWINGD 190
           L+HG G   +  GD   G W  G   G G  T ++ + Y+G  K G   G G   + NGD
Sbjct: 8   LRHGYGVYRFYTGDSYSGEWCNGQSHGVGLQTCSDASCYIGQFKYGVKHGVGCYHFRNGD 187

Query: 191 SYEGSWLNGMMHGFGAYTWSGGGCYVGTWTCGLKDGKGTF 230
            Y G +    +HGFG Y ++ G CY G W  G + G G++
Sbjct: 188 RYAGEYFGDKIHGFGVYHFANGHCYEGAWHEGSRQGIGSY 307



 Score = 78.2 bits (191), Expect = 1e-14
 Identities = 41/114 (35%), Positives = 57/114 (49%)
 Frame = +2

Query: 83  RGMRHGNGKIRWPYGAVYEGEFSGGYIQGTGKYIGSDHLTYKGRWRLNLKHGLGYQVYPN 142
           +G+RHG G  R+  G  Y GE+  G   G G    SD   Y G+++  +KHG+G   + N
Sbjct: 2   QGLRHGYGVYRFYTGDSYSGEWCNGQSHGVGLQTCSDASCYIGQFKYGVKHGVGCYHFRN 181

Query: 143 GDILEGSWIQGTPEGPGKYTWTNGNVYVGNMKGGTMSGKGTLTWINGDSYEGSW 196
           GD   G +      G G Y + NG+ Y G    G+  G G+ T+ NGD   G W
Sbjct: 182 GDRYAGEYFGDKIHGFGVYHFANGHCYEGAWHEGSRQGIGSYTFRNGDRRSGGW 343



 Score = 65.5 bits (158), Expect = 9e-11
 Identities = 35/103 (33%), Positives = 48/103 (45%)
 Frame = +2

Query: 51  GESYSGTLFGNTPEGHGTYVWSDRCVYEGGWRRGMRHGNGKIRWPYGAVYEGEFSGGYIQ 110
           G+SYSG        G G    SD   Y G ++ G++HG G   +  G  Y GE+ G  I 
Sbjct: 44  GDSYSGEWCNGQSHGVGLQTCSDASCYIGQFKYGVKHGVGCYHFRNGDRYAGEYFGDKIH 223

Query: 111 GTGKYIGSDHLTYKGRWRLNLKHGLGYQVYPNGDILEGSWIQG 153
           G G Y  ++   Y+G W    + G+G   + NGD   G W  G
Sbjct: 224 GFGVYHFANGHCYEGAWHEGSRQGIGSYTFRNGDRRSGGWDAG 352



 Score = 64.3 bits (155), Expect = 2e-10
 Identities = 36/114 (31%), Positives = 51/114 (44%)
 Frame = +2

Query: 65  GHGTYVWSDRCVYEGGWRRGMRHGNGKIRWPYGAVYEGEFSGGYIQGTGKYIGSDHLTYK 124
           G+G Y +     Y G W  G  HG G       + Y G+F  G   G G Y   +   Y 
Sbjct: 17  GYGVYRFYTGDSYSGEWCNGQSHGVGLQTCSDASCYIGQFKYGVKHGVGCYHFRNGDRYA 196

Query: 125 GRWRLNLKHGLGYQVYPNGDILEGSWIQGTPEGPGKYTWTNGNVYVGNMKGGTM 178
           G +  +  HG G   + NG   EG+W +G+ +G G YT+ NG+   G    G +
Sbjct: 197 GEYFGDKIHGFGVYHFANGHCYEGAWHEGSRQGIGSYTFRNGDRRSGGWDAGNL 358



 Score = 55.1 bits (131), Expect = 1e-07
 Identities = 23/56 (41%), Positives = 29/56 (51%)
 Frame = +2

Query: 176 GTMSGKGTLTWINGDSYEGSWLNGMMHGFGAYTWSGGGCYVGTWTCGLKDGKGTFY 231
           G   G G   +  GDSY G W NG  HG G  T S   CY+G +  G+K G G ++
Sbjct: 5   GLRHGYGVYRFYTGDSYSGEWCNGQSHGVGLQTCSDASCYIGQFKYGVKHGVGCYH 172



 Score = 43.5 bits (101), Expect = 4e-04
 Identities = 25/85 (29%), Positives = 34/85 (39%)
 Frame = +2

Query: 152 QGTPEGPGKYTWTNGNVYVGNMKGGTMSGKGTLTWINGDSYEGSWLNGMMHGFGAYTWSG 211
           QG   G G Y +  G+ Y G    G   G G  T  +   Y G +  G+ HG G Y +  
Sbjct: 2   QGLRHGYGVYRFYTGDSYSGEWCNGQSHGVGLQTCSDASCYIGQFKYGVKHGVGCYHFRN 181

Query: 212 GGCYVGTWTCGLKDGKGTFYPKGSH 236
           G  Y G +      G G ++    H
Sbjct: 182 GDRYAGEYFGDKIHGFGVYHFANGH 256



 Score = 43.5 bits (101), Expect = 4e-04
 Identities = 22/68 (32%), Positives = 32/68 (46%)
 Frame = +2

Query: 43  VGELLLSNGESYSGTLFGNTPEGHGTYVWSDRCVYEGGWRRGMRHGNGKIRWPYGAVYEG 102
           VG     NG+ Y+G  FG+   G G Y +++   YEG W  G R G G   +  G    G
Sbjct: 158 VGCYHFRNGDRYAGEYFGDKIHGFGVYHFANGHCYEGAWHEGSRQGIGSYTFRNGDRRSG 337

Query: 103 EFSGGYIQ 110
            +  G ++
Sbjct: 338 GWDAGNLK 361



 Score = 35.4 bits (80), Expect = 0.10
 Identities = 29/97 (29%), Positives = 36/97 (36%), Gaps = 1/97 (1%)
 Frame = +2

Query: 40  SFSVGELLLSNGESYSGTLFGNTPEGHGTYVWSDRCVYEGGWRRGMRHGNGKIRWPYGAV 99
           S  VG    S+   Y G        G G Y + +   Y G +     HG G   +  G  
Sbjct: 80  SHGVGLQTCSDASCYIGQFKYGVKHGVGCYHFRNGDRYAGEYFGDKIHGFGVYHFANGHC 259

Query: 100 YEGEFSGGYIQGTGKYIGSDHLTYKGRWRL-NLKHGL 135
           YEG +  G  QG G Y   +     G W   NLK  L
Sbjct: 260 YEGAWHEGSRQGIGSYTFRNGDRRSGGWDAGNLKQSL 370


>AW278716 weakly similar to GP|22775626|db 1-phosphatidylinositol-4-phosphate
           kinase-like~contains ESTs AU030318(E50902)
           AU030317(E50902), partial (13%)
          Length = 397

 Score = 76.6 bits (187), Expect = 4e-14
 Identities = 41/100 (41%), Positives = 48/100 (48%)
 Frame = +2

Query: 50  NGESYSGTLFGNTPEGHGTYVWSDRCVYEGGWRRGMRHGNGKIRWPYGAVYEGEFSGGYI 109
           N + Y+G+   N P G   Y+W D CVYE  W  G   G  K  WP  A YEGEF    +
Sbjct: 77  NCDVYTGSFLVNAPSGSKKYLWRD*CVYESEWNIGKACGK*KYSWPCVATYEGEFKSCRM 256

Query: 110 QGTGKYIGSDHLTYKGRWRLNLKHGLGYQVYPNGDILEGS 149
            G G   GSD  TY      N K G     Y NGD+ +GS
Sbjct: 257 DGFGSCTGSDSDTYHVF*SSNKKLGYSQNRYTNGDLNDGS 376



 Score = 48.1 bits (113), Expect = 2e-05
 Identities = 26/75 (34%), Positives = 35/75 (46%)
 Frame = +2

Query: 141 PNGDILEGSWIQGTPEGPGKYTWTNGNVYVGNMKGGTMSGKGTLTWINGDSYEGSWLNGM 200
           PN D+  GS++   P G  KY W +  VY      G   GK   +W    +YEG + +  
Sbjct: 74  PNCDVYTGSFLVNAPSGSKKYLWRD*CVYESEWNIGKACGK*KYSWPCVATYEGEFKSCR 253

Query: 201 MHGFGAYTWSGGGCY 215
           M GFG+ T S    Y
Sbjct: 254 MDGFGSCTGSDSDTY 298



 Score = 34.7 bits (78), Expect = 0.17
 Identities = 20/70 (28%), Positives = 30/70 (42%)
 Frame = +2

Query: 123 YKGRWRLNLKHGLGYQVYPNGDILEGSWIQGTPEGPGKYTWTNGNVYVGNMKGGTMSGKG 182
           Y G + +N   G    ++ +  + E  W  G   G  KY+W     Y G  K   M G G
Sbjct: 89  YTGSFLVNAPSGSKKYLWRD*CVYESEWNIGKACGK*KYSWPCVATYEGEFKSCRMDGFG 268

Query: 183 TLTWINGDSY 192
           + T  + D+Y
Sbjct: 269 SCTGSDSDTY 298


>TC212252 
          Length = 931

 Score = 50.4 bits (119), Expect(2) = 1e-11
 Identities = 24/39 (61%), Positives = 32/39 (81%), Gaps = 1/39 (2%)
 Frame = +2

Query: 772 KKLEHAYKSLQFDSVSISVVDPAFYSRRFLEFIQ-KVFP 809
           KKLEHA KSLQ+D ++ISVV+P  Y+ RF+ F++ KVFP
Sbjct: 719 KKLEHACKSLQYDPMTISVVEPKTYAERFINFMEKKVFP 835



 Score = 37.7 bits (86), Expect(2) = 1e-11
 Identities = 17/25 (68%), Positives = 22/25 (88%)
 Frame = +1

Query: 743 KQEKQMFHEVNDVVLYLGIIDILQD 767
           K++K+ F EV DVVLY+GIIDILQ+
Sbjct: 631 KKKKKKFFEVYDVVLYMGIIDILQE 705


>TC217753 weakly similar to UP|FAB1_YEAST (P34756)
           1-phosphatidylinositol-3-phosphate 5-kinase FAB1
           (Phosphatidylinositol-3-phosphate 5-kinase) (Type III
           PIP kinase) , partial (3%)
          Length = 1144

 Score = 67.4 bits (163), Expect = 2e-11
 Identities = 65/214 (30%), Positives = 108/214 (50%), Gaps = 20/214 (9%)
 Frame = +3

Query: 457 FRNLRELFKIDAADYMISI--CGN-DALRELSSPGKSGSVFFLSQDDRFMIKTLRKSELK 513
           FR LR        D++ S+  C N DA       GKS S F  + DDRF+IK ++K+EL 
Sbjct: 54  FRELRNWCCPSELDFIASLSRCRNWDA-----KGGKSKSYFAKTLDDRFIIKEIKKTELD 218

Query: 514 IFLRMLPDYHHHVK-SYEN---TLITKFFGLHRIKP---SSGQKFRF---AVMGNMFCTE 563
            FL     Y  HV+ S+E+   T + K  G++++      SG++ ++    VM N+    
Sbjct: 219 SFLGFSSLYFKHVRESFESGSQTCLAKVLGIYQVTKRHVKSGKEVKYDPLMVMENLTYNR 398

Query: 564 LRIHRRFDLKGSSFGR------SSDKIEIDENTTLKDLDLNYS-FFLEPSWRDSLLNQIK 616
             I R++DLKG+ + R       +  + +D+N      D+N S  ++    +  L   + 
Sbjct: 399 -NITRQYDLKGALYARYNSAADGAGDVLLDQNFV---NDMNSSPLYVSLKAKRYLQRAVW 566

Query: 617 IDSKFLEAQHIMDYSLLLGVHYRAPQHVRPLMSY 650
            D+ FL + ++MDYSLL+GV  +  + V  ++ Y
Sbjct: 567 NDTSFLNSINVMDYSLLVGVDSQKSELVCGIIDY 668


>TC213097 
          Length = 429

 Score = 66.6 bits (161), Expect = 4e-11
 Identities = 46/148 (31%), Positives = 71/148 (47%), Gaps = 2/148 (1%)
 Frame = +3

Query: 205 GAYTWSGGGCYVGTWTCGLKDGKGTFYPKGSHLTSAQEIYL-NALRKRGLLPDLRKQNLV 263
           G Y +  GG Y+GTW  GLKDGKG FYP GS   S ++++        G L ++ KQ   
Sbjct: 6   GVYRFGDGGLYIGTWNKGLKDGKGVFYPAGSKHPSLKKLHSPRDSDHNGFLLNVEKQEAP 185

Query: 264 RG-VKVGENQRSNRVSSDKLAKGNLLNLEDSSRRNVSLERRWSLEVSIEKGIGHDSTLGS 322
           +  VK   ++    +S  K       +    S R  SL+       S +  + HDS+L  
Sbjct: 186 KARVKRSLSENMPVISRFK-------SFRQISHRTSSLKINLIRSDSAQDCVCHDSSLTL 344

Query: 323 AESVSQRKDKEANAKMPILEREYMQGVL 350
           + ++   + + +     + EREY+QGVL
Sbjct: 345 SNALDGDQSEASGESTLVYEREYVQGVL 428


>TC205219 similar to GB|AAF16539.1|6552740|AC013482 T26F17.15 {Arabidopsis
           thaliana;} , partial (43%)
          Length = 730

 Score = 54.3 bits (129), Expect(2) = 2e-09
 Identities = 27/75 (36%), Positives = 34/75 (45%)
 Frame = +2

Query: 148 GSWIQGTPEGPGKYTWTNGNVYVGNMKGGTMSGKGTLTWINGDSYEGSWLNGMMHGFGAY 207
           G +  G   G G Y + NG+ Y G   G  + G G   + NG  YEGSW  G   G+G Y
Sbjct: 143 GEFKFGVKHGLGCYHFRNGDRYAGEYFGDKIHGFGVYHFANGHYYEGSWHEGRRQGYGMY 322

Query: 208 TWSGGGCYVGTWTCG 222
            +  G    G W  G
Sbjct: 323 MFRNGDGRCGEWDAG 367



 Score = 53.5 bits (127), Expect(2) = 6e-11
 Identities = 24/62 (38%), Positives = 32/62 (50%)
 Frame = +2

Query: 169 YVGNMKGGTMSGKGTLTWINGDSYEGSWLNGMMHGFGAYTWSGGGCYVGTWTCGLKDGKG 228
           YVG  K G   G G   + NGD Y G +    +HGFG Y ++ G  Y G+W  G + G G
Sbjct: 137 YVGEFKFGVKHGLGCYHFRNGDRYAGEYFGDKIHGFGVYHFANGHYYEGSWHEGRRQGYG 316

Query: 229 TF 230
            +
Sbjct: 317 MY 322



 Score = 49.7 bits (117), Expect = 5e-06
 Identities = 26/77 (33%), Positives = 38/77 (48%)
 Frame = +2

Query: 77  YEGGWRRGMRHGNGKIRWPYGAVYEGEFSGGYIQGTGKYIGSDHLTYKGRWRLNLKHGLG 136
           Y G ++ G++HG G   +  G  Y GE+ G  I G G Y  ++   Y+G W    + G G
Sbjct: 137 YVGEFKFGVKHGLGCYHFRNGDRYAGEYFGDKIHGFGVYHFANGHYYEGSWHEGRRQGYG 316

Query: 137 YQVYPNGDILEGSWIQG 153
             ++ NGD   G W  G
Sbjct: 317 MYMFRNGDGRCGEWDAG 367



 Score = 48.9 bits (115), Expect(2) = 9e-07
 Identities = 27/79 (34%), Positives = 36/79 (45%)
 Frame = +2

Query: 100 YEGEFSGGYIQGTGKYIGSDHLTYKGRWRLNLKHGLGYQVYPNGDILEGSWIQGTPEGPG 159
           Y GEF  G   G G Y   +   Y G +  +  HG G   + NG   EGSW +G  +G G
Sbjct: 137 YVGEFKFGVKHGLGCYHFRNGDRYAGEYFGDKIHGFGVYHFANGHYYEGSWHEGRRQGYG 316

Query: 160 KYTWTNGNVYVGNMKGGTM 178
            Y + NG+   G    G +
Sbjct: 317 MYMFRNGDGRCGEWDAGNL 373



 Score = 44.3 bits (103), Expect = 2e-04
 Identities = 21/61 (34%), Positives = 31/61 (50%)
 Frame = +2

Query: 50  NGESYSGTLFGNTPEGHGTYVWSDRCVYEGGWRRGMRHGNGKIRWPYGAVYEGEFSGGYI 109
           NG+ Y+G  FG+   G G Y +++   YEG W  G R G G   +  G    GE+  G +
Sbjct: 194 NGDRYAGEYFGDKIHGFGVYHFANGHYYEGSWHEGRRQGYGMYMFRNGDGRCGEWDAGNL 373

Query: 110 Q 110
           +
Sbjct: 374 K 376



 Score = 39.7 bits (91), Expect = 0.005
 Identities = 19/52 (36%), Positives = 23/52 (43%)
 Frame = +3

Query: 171 GNMKGGTMSGKGTLTWINGDSYEGSWLNGMMHGFGAYTWSGGGCYVGTWTCG 222
           G  K G   G G   +  GDSY G W NG  HG G    S     +G+ + G
Sbjct: 6   GFYKQGLRHGFGVYKFYTGDSYAGEWCNGQSHGVGVQACSDAVAMLGSLSLG 161



 Score = 35.8 bits (81), Expect = 0.078
 Identities = 15/36 (41%), Positives = 18/36 (49%)
 Frame = +3

Query: 193 EGSWLNGMMHGFGAYTWSGGGCYVGTWTCGLKDGKG 228
           EG +  G+ HGFG Y +  G  Y G W  G   G G
Sbjct: 3   EGFYKQGLRHGFGVYKFYTGDSYAGEWCNGQSHGVG 110



 Score = 32.3 bits (72), Expect(2) = 6e-11
 Identities = 14/54 (25%), Positives = 25/54 (45%)
 Frame = +3

Query: 124 KGRWRLNLKHGLGYQVYPNGDILEGSWIQGTPEGPGKYTWTNGNVYVGNMKGGT 177
           +G ++  L+HG G   +  GD   G W  G   G G    ++    +G++  G+
Sbjct: 3   EGFYKQGLRHGFGVYKFYTGDSYAGEWCNGQSHGVGVQACSDAVAMLGSLSLGS 164



 Score = 26.6 bits (57), Expect(2) = 2e-09
 Identities = 19/77 (24%), Positives = 31/77 (39%)
 Frame = +3

Query: 78  EGGWRRGMRHGNGKIRWPYGAVYEGEFSGGYIQGTGKYIGSDHLTYKGRWRLNLKHGLGY 137
           EG +++G+RHG G  ++     Y G+                  +Y G W     HG+G 
Sbjct: 3   EGFYKQGLRHGFGVYKF-----YTGD------------------SYAGEWCNGQSHGVGV 113

Query: 138 QVYPNGDILEGSWIQGT 154
           Q   +   + GS   G+
Sbjct: 114 QACSDAVAMLGSLSLGS 164



 Score = 22.7 bits (47), Expect(2) = 9e-07
 Identities = 10/26 (38%), Positives = 11/26 (41%)
 Frame = +3

Query: 65 GHGTYVWSDRCVYEGGWRRGMRHGNG 90
          G G Y +     Y G W  G  HG G
Sbjct: 33 GFGVYKFYTGDSYAGEWCNGQSHGVG 110


>TC218384 similar to GB|AAQ56792.1|34098819|BT010349 At1g71010 {Arabidopsis
           thaliana;} , partial (30%)
          Length = 857

 Score = 64.7 bits (156), Expect = 2e-10
 Identities = 53/164 (32%), Positives = 81/164 (49%), Gaps = 15/164 (9%)
 Frame = +3

Query: 488 GKSGSVFFLSQDDRFMIKTLRKSELKIFLRMLPDYHHHVKSYEN----TLITKFFGLHRI 543
           GKS   F  S D+RF+IK + K+EL+ F    P Y  ++    N    T + K  G++++
Sbjct: 18  GKSNVYFAKSLDERFIIKQVTKTELESFEEFAPQYFKYLMDALNSGGPTCLAKILGIYQV 197

Query: 544 K---PSSGQ--KFRFAVMGNMFCTELRIHRRFDLKGSSFGR------SSDKIEIDENTTL 592
               P  G+  K    VM N+F  + +I R +DLKGS   R       ++K+ +D N  L
Sbjct: 198 TVKYPKGGKETKIDLMVMENLF-YKRKISRIYDLKGSERSRYNPDTTGTNKVMLDMN--L 368

Query: 593 KDLDLNYSFFLEPSWRDSLLNQIKIDSKFLEAQHIMDYSLLLGV 636
            +       FL    +  L   +  D+ FL +  +MDYSLL+GV
Sbjct: 369 LETLRTKPIFLGSRAKRILERAVWNDTSFLASVDVMDYSLLVGV 500


>TC227744 similar to UP|Q9LUM0 (Q9LUM0) Emb|CAB36798.1, partial (19%)
          Length = 1450

 Score = 64.3 bits (155), Expect = 2e-10
 Identities = 51/164 (31%), Positives = 80/164 (48%), Gaps = 15/164 (9%)
 Frame = +2

Query: 488 GKSGSVFFLSQDDRFMIKTLRKSELKIFLRMLPDYHHH----VKSYENTLITKFFGLHRI 543
           GKS   F  + DDRF+IK + K+EL+ F +  P Y  +    + +   T + K  G++++
Sbjct: 311 GKSNVFFAKTLDDRFIIKQVTKTELESFTKFAPAYFKYLSESISTGSPTCLAKILGIYQV 490

Query: 544 -----KPSSGQKFRFAVMGNMFCTELRIHRRFDLKGSSFGR------SSDKIEIDENTTL 592
                K     K    VM N+      I R +DLKGSS  R       S+K+ +D+N  L
Sbjct: 491 TSKHLKGGKETKMDVLVMENLLYRR-NIRRLYDLKGSSRSRYNPDTSGSNKVLLDQN--L 661

Query: 593 KDLDLNYSFFLEPSWRDSLLNQIKIDSKFLEAQHIMDYSLLLGV 636
            +       F+    +  L   +  D+ FL + ++MDYSLL+GV
Sbjct: 662 IEAMPTSPIFVGNKAKRLLERAVWNDTAFLASIYVMDYSLLVGV 793


>TC227161 similar to UP|Q9LUM0 (Q9LUM0) Emb|CAB36798.1, partial (23%)
          Length = 1726

 Score = 60.1 bits (144), Expect = 4e-09
 Identities = 48/164 (29%), Positives = 80/164 (48%), Gaps = 15/164 (9%)
 Frame = +3

Query: 488 GKSGSVFFLSQDDRFMIKTLRKSELKIFLRMLPDYHHH----VKSYENTLITKFFGLHRI 543
           GKS   F  + DDRF+IK + K+EL+ F++  P+Y  +    + +   T + K  G++++
Sbjct: 621 GKSNVFFAKTLDDRFIIKQVTKTELESFIKFGPEYFKYLSESIGTGSPTCLAKILGIYQV 800

Query: 544 -----KPSSGQKFRFAVMGNMFCTELRIHRRFDLKGSSFGR------SSDKIEIDENTTL 592
                K     +    VM N+      + R +DLKGSS  R        +K+ +D+N  L
Sbjct: 801 TSKHLKGGKESRMDVLVMENLLFRR-TVTRLYDLKGSSRSRYNADSTGKNKVLLDQN--L 971

Query: 593 KDLDLNYSFFLEPSWRDSLLNQIKIDSKFLEAQHIMDYSLLLGV 636
            +       F+    +  L   +  D+ FL +  +MDYSLL+GV
Sbjct: 972 IEAMPTSPIFVGNKAKRLLERAVWNDTGFLASVDVMDYSLLVGV 1103


>BM886461 
          Length = 422

 Score = 54.7 bits (130), Expect = 2e-07
 Identities = 23/57 (40%), Positives = 31/57 (54%)
 Frame = +2

Query: 163 WTNGNVYVGNMKGGTMSGKGTLTWINGDSYEGSWLNGMMHGFGAYTWSGGGCYVGTW 219
           + NG+VY G +KGG   G G   +     YEG W++G   GFG  TW+ G  Y G +
Sbjct: 251 YANGDVYEGELKGGKCCGSGVYYYSMSGRYEGDWVDGKYDGFGVETWARGSRYRGQY 421



 Score = 41.2 bits (95), Expect = 0.002
 Identities = 21/56 (37%), Positives = 27/56 (47%)
 Frame = +2

Query: 49  SNGESYSGTLFGNTPEGHGTYVWSDRCVYEGGWRRGMRHGNGKIRWPYGAVYEGEF 104
           +NG+ Y G L G    G G Y +S    YEG W  G   G G   W  G+ Y G++
Sbjct: 254 ANGDVYEGELKGGKCCGSGVYYYSMSGRYEGDWVDGKYDGFGVETWARGSRYRGQY 421



 Score = 38.5 bits (88), Expect = 0.012
 Identities = 19/43 (44%), Positives = 21/43 (48%)
 Frame = +2

Query: 186 WINGDSYEGSWLNGMMHGFGAYTWSGGGCYVGTWTCGLKDGKG 228
           + NGD YEG    G   G G Y +S  G Y G W  G  DG G
Sbjct: 251 YANGDVYEGELKGGKCCGSGVYYYSMSGRYEGDWVDGKYDGFG 379



 Score = 38.5 bits (88), Expect = 0.012
 Identities = 26/79 (32%), Positives = 33/79 (40%), Gaps = 2/79 (2%)
 Frame = +2

Query: 95  PY--GAVYEGEFSGGYIQGTGKYIGSDHLTYKGRWRLNLKHGLGYQVYPNGDILEGSWIQ 152
           PY  G VYEGE  GG   G+G Y    + +  GR+                   EG W+ 
Sbjct: 248 PYANGDVYEGELKGGKCCGSGVY----YYSMSGRY-------------------EGDWVD 358

Query: 153 GTPEGPGKYTWTNGNVYVG 171
           G  +G G  TW  G+ Y G
Sbjct: 359 GKYDGFGVETWARGSRYRG 415


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.318    0.137    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,913,340
Number of Sequences: 63676
Number of extensions: 530440
Number of successful extensions: 2580
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 2457
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2558
length of query: 815
length of database: 12,639,632
effective HSP length: 105
effective length of query: 710
effective length of database: 5,953,652
effective search space: 4227092920
effective search space used: 4227092920
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)


Lotus: description of TM0012b.5