BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0010.3
(172 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC224974 30 0.56
TC213853 similar to UP|Q7Z8E9 (Q7Z8E9) PAK kinase, partial (9%) 30 0.56
BF595056 30 0.56
TC227435 similar to UP|Q96332 (Q96332) Glyoxalase II, complete 29 0.96
AW458311 28 1.6
CD395996 28 2.1
TC227193 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alp... 28 2.8
TC220348 homologue to UP|SECA_PEA (Q41062) Preprotein translocas... 27 3.6
BG155795 weakly similar to GP|18139887|gb O-linked N-acetyl gluc... 27 3.6
CA819108 27 4.8
TC215498 homologue to UP|Q8VZC4 (Q8VZC4) Proteasome subunit, com... 27 6.2
TC215499 homologue to UP|PS32_ARATH (O81153) Proteasome subunit ... 27 6.2
TC210463 similar to UP|Q9LS38 (Q9LS38) Arabidopsis thaliana geno... 26 8.1
TC226613 similar to UP|Q8H0V8 (Q8H0V8) Spliceosome associated pr... 26 8.1
>TC224974
Length = 714
Score = 30.0 bits (66), Expect = 0.56
Identities = 23/74 (31%), Positives = 37/74 (49%), Gaps = 2/74 (2%)
Frame = +2
Query: 23 EEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNP--SGEALEVRFCTVLPNLLHAY 80
+EEEEEEE + +LPE + +S + P +G L +V N A
Sbjct: 143 DEEEEEEEIKLKQKKQRLKETLPEVLNRIASAILFQEPADAGSLLRRIKVSVADN---AT 313
Query: 81 VVPSSSRNNDREVI 94
++P +SRN+ R+V+
Sbjct: 314 LLPEASRNSARDVL 355
>TC213853 similar to UP|Q7Z8E9 (Q7Z8E9) PAK kinase, partial (9%)
Length = 600
Score = 30.0 bits (66), Expect = 0.56
Identities = 28/95 (29%), Positives = 43/95 (44%), Gaps = 12/95 (12%)
Frame = -2
Query: 54 TLTPNNPSGEALEVRFCT----VLPNLLHAYV--VPSSSRNNDREVIAVVKL------IS 101
T PN PSG A+ ++ CT L L + P +SRN + + + + IS
Sbjct: 290 TSGPNEPSGSAVWIKGCTRRS*ALTRLSGSTFSEKPKNSRNTGDKACSSLNVGVPLVTIS 111
Query: 102 HTARNFPGVFFLGKPSAVLPVLADILPSYADPLFR 136
TARN + + G PS + D++P+ FR
Sbjct: 110 QTARNRLSIRYGGSPSI---ISMDMIPNDQMSTFR 15
>BF595056
Length = 421
Score = 30.0 bits (66), Expect = 0.56
Identities = 22/67 (32%), Positives = 33/67 (48%)
Frame = -3
Query: 21 VREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAY 80
+R EEEEEEE FV + + + +P SSS LT E F T+L +++ A
Sbjct: 239 LRREEEEEEEGVFVFVFEFKMVMMP-CFMISSSILTEEEEEEEDKRPLF*TLLLSVIGAD 63
Query: 81 VVPSSSR 87
+ + R
Sbjct: 62 DITTQRR 42
>TC227435 similar to UP|Q96332 (Q96332) Glyoxalase II, complete
Length = 1220
Score = 29.3 bits (64), Expect = 0.96
Identities = 19/49 (38%), Positives = 24/49 (48%)
Frame = -2
Query: 20 RVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEVR 68
R + EEEEEE + NL L + IA + T T GEALE +
Sbjct: 250 RNKSLEEEEEEVEKEPICDLNLVGLLQTIALETVTETGVRGKGEALEAK 104
>AW458311
Length = 392
Score = 28.5 bits (62), Expect = 1.6
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = -1
Query: 39 ANLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNL 76
++++ LPER A+ S TLTP PS L + C +P L
Sbjct: 122 SDITWLPER*ASVSVTLTP--PSAATLFLSLCPAIPPL 15
>CD395996
Length = 638
Score = 28.1 bits (61), Expect = 2.1
Identities = 18/53 (33%), Positives = 25/53 (46%)
Frame = -3
Query: 12 TFPHSTLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEA 64
T P S ++R + EEEA F ++A A S P + S S + P G A
Sbjct: 594 TPPASLVLRFLDGLSTEEEAPFTALATALSKSWPSSSSVSESLSSITEPGGGA 436
>TC227193 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (41%)
Length = 557
Score = 27.7 bits (60), Expect = 2.8
Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
Frame = +1
Query: 35 SMAKANLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAYVVPSSSRNN---DR 91
+MAK S LP R ++SSSTL L F ++ L +PSSSR N
Sbjct: 70 TMAKPRYSRLPPRKSSSSSTLILT----LFLVFTFLVLILLALGILSIPSSSRGNLPKPN 237
Query: 92 EVIAVVKLISHTA 104
++ ++ + HT+
Sbjct: 238 DLASIARNTIHTS 276
>TC220348 homologue to UP|SECA_PEA (Q41062) Preprotein translocase secA
subunit, chloroplast precursor, partial (25%)
Length = 776
Score = 27.3 bits (59), Expect = 3.6
Identities = 27/87 (31%), Positives = 41/87 (47%), Gaps = 7/87 (8%)
Frame = -3
Query: 31 AYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEVRF-------CTVLPNLLHAYVVP 83
A V++ A L I+AS ST SG AL C+ +L VVP
Sbjct: 585 AILVAIVTAPRRPLCATISASLSTF-----SGFALSTSCGIPASFNCSDKESLCSTLVVP 421
Query: 84 SSSRNNDREVIAVVKLISHTARNFPGV 110
+S+ R V+ ++++ + TAR+FP V
Sbjct: 420 TST---GRPVLCILEISTTTARHFPEV 349
>BG155795 weakly similar to GP|18139887|gb O-linked N-acetyl glucosamine
transferase {Arabidopsis thaliana}, partial (13%)
Length = 571
Score = 27.3 bits (59), Expect = 3.6
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 8/70 (11%)
Frame = +2
Query: 61 SGEALEVRFCTVLPNLLHAYVVPSSSRNNDR--------EVIAVVKLISHTARNFPGVFF 112
SGE + VR+ V L H Y++ NND + +V +++H+ + V +
Sbjct: 110 SGETILVRYDAVQGVLPHHYILTDQGTNNDHIR*SC*AYSFLDIVFMLAHSTGAY--VLW 283
Query: 113 LGKPSAVLPV 122
+P LP+
Sbjct: 284 ASEPMVSLPL 313
>CA819108
Length = 474
Score = 26.9 bits (58), Expect = 4.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 47 RIAASSSTLTPNNPSGEALEVRFCT 71
RI AS TP +P+ LEV FC+
Sbjct: 194 RITASCPDSTPRSPATSLLEVAFCS 120
>TC215498 homologue to UP|Q8VZC4 (Q8VZC4) Proteasome subunit, complete
Length = 967
Score = 26.6 bits (57), Expect = 6.2
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -1
Query: 78 HAYVVPSSSRNNDREVIAVVKLISHT 103
H +V+PS S N+D ++IAV+ S T
Sbjct: 643 HRHVLPSHSNNHDLQMIAVLGRWSQT 566
>TC215499 homologue to UP|PS32_ARATH (O81153) Proteasome subunit beta type
3-2 (20S proteasome alpha subunit C2) , partial (43%)
Length = 520
Score = 26.6 bits (57), Expect = 6.2
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -2
Query: 78 HAYVVPSSSRNNDREVIAVVKLISHT 103
H +V+PS S N+D ++IAV+ S T
Sbjct: 213 HRHVLPSHSNNHDLQMIAVLGRWSQT 136
>TC210463 similar to UP|Q9LS38 (Q9LS38) Arabidopsis thaliana genomic DNA,
chromosome 3, P1 clone: MYF24, partial (6%)
Length = 770
Score = 26.2 bits (56), Expect = 8.1
Identities = 10/26 (38%), Positives = 17/26 (64%)
Frame = -2
Query: 102 HTARNFPGVFFLGKPSAVLPVLADIL 127
HT+ N P VFF+G SA++ + ++
Sbjct: 646 HTSLNLPQVFFVGAESALISHITQLV 569
>TC226613 similar to UP|Q8H0V8 (Q8H0V8) Spliceosome associated protein-like
(At4g21660), partial (59%)
Length = 1844
Score = 26.2 bits (56), Expect = 8.1
Identities = 24/87 (27%), Positives = 36/87 (40%), Gaps = 21/87 (24%)
Frame = +2
Query: 23 EEEEEEEEAYFVSMAKANLSSLPERIAASSSTLT-----------------PNNPSGEAL 65
EEEEEEEE M + L + + + + SST T P P + L
Sbjct: 878 EEEEEEEEEEEEEMEEEELEAGIQSVDSLSSTPTGVETPDVIDLRKQQRKEPERPLYQVL 1057
Query: 66 EVRFCTVLPNLL----HAYVVPSSSRN 88
E + + P L H YVV + +++
Sbjct: 1058EEKEEKIAPGTLLGTTHTYVVGTGTQD 1138
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.135 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,531,134
Number of Sequences: 63676
Number of extensions: 105250
Number of successful extensions: 781
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 701
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 762
length of query: 172
length of database: 12,639,632
effective HSP length: 91
effective length of query: 81
effective length of database: 6,845,116
effective search space: 554454396
effective search space used: 554454396
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0010.3