BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0005.13
(396 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC215894 similar to UP|Q96569 (Q96569) L-lactate dehydrogenase ... 38 0.009
TC226467 weakly similar to UP|Q8L685 (Q8L685) Pherophorin-dz1 pr... 36 0.027
TC211106 similar to UP|RS2_HUMAN (P15880) 40S ribosomal protein ... 36 0.035
TC225560 similar to UP|Q9FUL8 (Q9FUL8) Arabinogalactan protein, ... 35 0.045
TC220043 weakly similar to UP|Q940C3 (Q940C3) AT3g27530/MMJ24_7,... 35 0.059
TC218285 weakly similar to UP|Q9SKM6 (Q9SKM6) En/Spm-like transp... 35 0.077
TC219582 similar to UP|Q93ZQ3 (Q93ZQ3) AT3g63200/F16M2_50, parti... 34 0.10
TC227925 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein ... 34 0.13
TC220200 weakly similar to UP|Q7M4A3 (Q7M4A3) Spermatid-specific... 34 0.13
TC225579 similar to UP|Q84KS7 (Q84KS7) AP2/ERF-domain protein, p... 34 0.13
TC220114 weakly similar to UP|PRT1_SEPOF (P80001) Spermatid-spec... 33 0.17
BI973751 33 0.23
TC220592 weakly similar to UP|Q9LUC0 (Q9LUC0) Gb|AAD10662.1, par... 33 0.23
TC226353 weakly similar to UP|Q9M510 (Q9M510) Dicyanin, partial ... 33 0.23
TC214748 homologue to UP|Q39882 (Q39882) Nodulin-26, partial (98%) 33 0.29
TC204808 weakly similar to UP|HQGT_RAUSE (Q9AR73) Hydroquinone g... 33 0.29
TC209651 weakly similar to UP|Q9C887 (Q9C887) Myosin-like protei... 33 0.29
TC203681 similar to UP|Q9XIV1 (Q9XIV1) Cucumis sativus mRNA expr... 33 0.29
BF068717 33 0.29
TC228623 similar to UP|Q9SA21 (Q9SA21) F3O9.2 protein, partial (... 32 0.38
>TC215894 similar to UP|Q96569 (Q96569) L-lactate dehydrogenase , complete
Length = 1675
Score = 37.7 bits (86), Expect = 0.009
Identities = 22/71 (30%), Positives = 27/71 (37%)
Frame = +1
Query: 170 TSSSDGDRAPVISAAFCGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLSAKGFANR 229
TSSS P SAA C TP P+ P S P S PP A+ A+
Sbjct: 346 TSSSSSTPTPTSSAARCSTSSTPPPSSPAPRSTPPPTPPSPPAPTSASSPPAPARSPASH 525
Query: 230 EPPYSLSPTPA 240
S +P+
Sbjct: 526 ASTSSRGTSPS 558
>TC226467 weakly similar to UP|Q8L685 (Q8L685) Pherophorin-dz1 protein
precursor, partial (6%)
Length = 1017
Score = 36.2 bits (82), Expect = 0.027
Identities = 27/96 (28%), Positives = 38/96 (39%)
Frame = +3
Query: 146 PQVSEGLLTVFEKKGNVSPHPVAATSSSDGDRAPVISAAFCGPEETPKPNQPGEPSLATP 205
P S+ V K V+P P + + +AP P +TP+P P + TP
Sbjct: 180 PTTSQPPAIVAPKPAPVTP-PAPKVAPASSPKAP--------PPQTPQPQPPKVSPVVTP 332
Query: 206 VSSPRAQDDSVDKPPLSAKGFANREPPYSLSPTPAQ 241
S P V PP PP ++PTPA+
Sbjct: 333 TSPPPIPPPPVSTPP--------PLPPPKIAPTPAK 416
>TC211106 similar to UP|RS2_HUMAN (P15880) 40S ribosomal protein S2 (S4)
(LLREP3 protein), partial (6%)
Length = 978
Score = 35.8 bits (81), Expect = 0.035
Identities = 20/53 (37%), Positives = 26/53 (48%)
Frame = +2
Query: 188 PEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLSAKGFANREPPYSLSPTPA 240
P+ PKP+ S + SS + +V PPL+A A PP S PTPA
Sbjct: 20 PDTRPKPSPKSAASTSPTSSSAVSTPPAVACPPLTAAAAAPSSPPGSSPPTPA 178
>TC225560 similar to UP|Q9FUL8 (Q9FUL8) Arabinogalactan protein, partial
(48%)
Length = 1030
Score = 35.4 bits (80), Expect = 0.045
Identities = 25/80 (31%), Positives = 36/80 (44%)
Frame = +2
Query: 163 SPHPVAATSSSDGDRAPVISAAFCGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLS 222
+P AA + + P A P+ +P + P +P+ ATP SSP A + P
Sbjct: 161 TPATPAAATPAQAPSTPNSPAPVASPKSSPPASSP-KPATATPASSPAASPTTTPAAPAP 337
Query: 223 AKGFANREPPYSLSPTPAQV 242
A +PP + SP PA V
Sbjct: 338 A-----TKPP-AASPPPAPV 379
>TC220043 weakly similar to UP|Q940C3 (Q940C3) AT3g27530/MMJ24_7, partial
(46%)
Length = 1029
Score = 35.0 bits (79), Expect = 0.059
Identities = 37/151 (24%), Positives = 65/151 (42%), Gaps = 5/151 (3%)
Frame = +1
Query: 242 VARMDEVVKQQGLDRVSEGAFATAFHAFHH-YKRSAQDAEVLRSEVARLRA---LNAGFV 297
+AR Q RVS + +A + +++ E+L++E A + + N
Sbjct: 367 LARTGGGSNSQSEQRVSGSSDKVQINALSRDLQETSKRLEMLKAEKAEVESEARKNRTLA 546
Query: 298 EERKAFLAQLKAKETSFAKELAEQSSRAEVSLEFRDRKISDLEKALGKLRQEAQEESEAK 357
E+ +A L L S + EQ + + DLE + R+EAQ+ESE +
Sbjct: 547 EKMEADLRSLSGAYNSLEQSNIEQEKQVKALKSGAPSTFLDLEAIKAEAREEAQKESEGE 726
Query: 358 V-DLVASKDQTLSSMETKLEHLRAELAKKDE 387
+ DL+ L ++K++ L A L + E
Sbjct: 727 LNDLLV----CLGQEQSKVDRLSARLLELGE 807
>TC218285 weakly similar to UP|Q9SKM6 (Q9SKM6) En/Spm-like transposon
protein, partial (18%)
Length = 765
Score = 34.7 bits (78), Expect = 0.077
Identities = 26/86 (30%), Positives = 37/86 (42%), Gaps = 7/86 (8%)
Frame = +1
Query: 181 ISAAFCGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLSAKGFANREPPY------- 233
I+A P TPKP+ P PS ++P S+P + + PP ++ PP
Sbjct: 181 IAAESPAPAPTPKPDLP--PS-SSPTSAPASNPSPANSPPSPTPAPSHHSPPAPSPGTNP 351
Query: 234 SLSPTPAQVARMDEVVKQQGLDRVSE 259
S SP PA + V G+ V E
Sbjct: 352 SPSPAPAPADDAHDGVNHAGMGGVDE 429
>TC219582 similar to UP|Q93ZQ3 (Q93ZQ3) AT3g63200/F16M2_50, partial (29%)
Length = 768
Score = 34.3 bits (77), Expect = 0.10
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +3
Query: 181 ISAAFCGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKP 219
+SA C P ETP + P + +T S+P D+VD P
Sbjct: 225 VSAGLCTPPETPSTSSPKTTANSTSRSAPACSADAVDSP 341
>TC227925 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein precursor,
partial (88%)
Length = 1035
Score = 33.9 bits (76), Expect = 0.13
Identities = 23/61 (37%), Positives = 29/61 (46%), Gaps = 3/61 (4%)
Frame = +2
Query: 186 CGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPL--SAKGFANREP-PYSLSPTPAQV 242
CGP +P P P P+ A+P S+P + S P +A G A P P SP PA
Sbjct: 134 CGPASSPTPATPSSPAAASP-SAPSPTNPSPSPTPTGPAASGPAPAAPTPAPPSPAPAAT 310
Query: 243 A 243
A
Sbjct: 311 A 313
>TC220200 weakly similar to UP|Q7M4A3 (Q7M4A3) Spermatid-specific protein T2
precursor, partial (40%)
Length = 506
Score = 33.9 bits (76), Expect = 0.13
Identities = 25/85 (29%), Positives = 35/85 (40%), Gaps = 7/85 (8%)
Frame = +2
Query: 178 APVISAAFCGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLSAKGFANREPPY---- 233
A + A P TP P+ P PS + P SP + + + PP ++ PP
Sbjct: 185 AATAATAAESPAPTPTPDFP--PSSSPPFPSPASNPSTANFPPSPTPAPSHHSPPAPSPS 358
Query: 234 ---SLSPTPAQVARMDEVVKQQGLD 255
S SP PA V + V G+D
Sbjct: 359 SNPSPSPAPAPVDDAHDGVSHAGVD 433
>TC225579 similar to UP|Q84KS7 (Q84KS7) AP2/ERF-domain protein, partial (31%)
Length = 1156
Score = 33.9 bits (76), Expect = 0.13
Identities = 21/68 (30%), Positives = 31/68 (44%), Gaps = 5/68 (7%)
Frame = +3
Query: 181 ISAAFCGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLSAKGFANREPP-----YSL 235
I A+ G +P P +P EP+ P +S + S +PPL R PP +L
Sbjct: 435 IRASASGSAPSPPPRKPPEPTTTPPSASAATRPSSTSRPPL-------RHPPKNNAASAL 593
Query: 236 SPTPAQVA 243
+P P + A
Sbjct: 594 TPPPNKAA 617
>TC220114 weakly similar to UP|PRT1_SEPOF (P80001) Spermatid-specific protein
T1 [Contains: Sperm protamine SP1], partial (76%)
Length = 674
Score = 33.5 bits (75), Expect = 0.17
Identities = 23/64 (35%), Positives = 29/64 (44%), Gaps = 15/64 (23%)
Frame = -1
Query: 163 SPHPVAATSSSDGDRA-PVISAAFCGPEETPKPNQ--------------PGEPSLATPVS 207
SP P A TSS G A P SAA P P++ PG P++A P++
Sbjct: 350 SPSPSARTSSPSG*PAPPTPSAAALASTSAPTPSRRSRRRSSDDRTPACPGSPTMAAPIA 171
Query: 208 SPRA 211
SP A
Sbjct: 170 SPAA 159
>BI973751
Length = 430
Score = 33.1 bits (74), Expect = 0.23
Identities = 20/55 (36%), Positives = 25/55 (45%), Gaps = 5/55 (9%)
Frame = +3
Query: 205 PVSSPRAQDDSVDKPPLSAKGFANREPPYSLS-----PTPAQVARMDEVVKQQGL 254
P+SS R D +DKP + G EP SL TP Q+ RM E + L
Sbjct: 72 PISSKRIVPDQIDKPDWAGDGTPKIEPNSSLQHTIEVKTPDQIERMRETCRVSSL 236
>TC220592 weakly similar to UP|Q9LUC0 (Q9LUC0) Gb|AAD10662.1, partial (14%)
Length = 1166
Score = 33.1 bits (74), Expect = 0.23
Identities = 30/122 (24%), Positives = 59/122 (47%), Gaps = 6/122 (4%)
Frame = +1
Query: 272 YKRSAQ-DAEV-----LRSEVARLRALNAGFVEERKAFLAQLKAKETSFAKELAEQSSRA 325
Y++S + DAEV + +E+ R+R ER AQL A E+ AK AE
Sbjct: 334 YEKSLKVDAEVRAVAAISAELDRVRTDVQELAAERNELAAQLHAVESELAKARAEALFVP 513
Query: 326 EVSLEFRDRKISDLEKALGKLRQEAQEESEAKVDLVASKDQTLSSMETKLEHLRAELAKK 385
+ + + ++++ + E + + + ++ + D + M+ ++E LRAELA +
Sbjct: 514 AIKADIETMR-HEIQRGRNAIEFEKKTHA-SNLEHRRTMDNNMIIMDHEVEKLRAELANE 687
Query: 386 DE 387
++
Sbjct: 688 EK 693
>TC226353 weakly similar to UP|Q9M510 (Q9M510) Dicyanin, partial (26%)
Length = 1341
Score = 33.1 bits (74), Expect = 0.23
Identities = 20/58 (34%), Positives = 25/58 (42%)
Frame = +3
Query: 183 AAFCGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLSAKGFANREPPYSLSPTPA 240
A+ GP P+P G P A+PV + Q S PP SA P + S PA
Sbjct: 456 ASSTGPSPAPQPRGSGSPPRASPVPTQAPQASSPTPPPRSAPA-----PAFGPSSEPA 614
>TC214748 homologue to UP|Q39882 (Q39882) Nodulin-26, partial (98%)
Length = 1254
Score = 32.7 bits (73), Expect = 0.29
Identities = 24/74 (32%), Positives = 29/74 (38%)
Frame = +1
Query: 164 PHPVAATSSSDGDRAPVISAAFCGPEETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLSA 223
P P+ + SSS AP P T P P PS A +SP + S P SA
Sbjct: 313 PSPMLSPSSSPSPSAPT------SPAATSTPPSPSAPSWA--ATSPYSAASSTSSPSSSA 468
Query: 224 KGFANREPPYSLSP 237
+ P SL P
Sbjct: 469 PSWPPSSSPSSLPP 510
>TC204808 weakly similar to UP|HQGT_RAUSE (Q9AR73) Hydroquinone
glucosyltransferase (Arbutin synthase) , partial (25%)
Length = 431
Score = 32.7 bits (73), Expect = 0.29
Identities = 31/127 (24%), Positives = 49/127 (38%), Gaps = 1/127 (0%)
Frame = +3
Query: 164 PHPVAATSSSDGDRAPVISAAFCGPE-ETPKPNQPGEPSLATPVSSPRAQDDSVDKPPLS 222
P +T S +P S+ P T PN P+ +TP P + S PP +
Sbjct: 33 PSAKPSTPSPPHTPSPPSSSTSSPPTPSTSPPNSMPPPTSSTPPQPPSSPSPSTS-PPST 209
Query: 223 AKGFANREPPYSLSPTPAQVARMDEVVKQQGLDRVSEGAFATAFHAFHHYKRSAQDAEVL 282
++ A+ E + SP P + + + LD V E HH KR + ++
Sbjct: 210 SRSSASSETSRNRSPIPGCI----PLPVKDFLDPVLERTNEAYKWVLHHSKRYREAEGII 377
Query: 283 RSEVARL 289
+ A L
Sbjct: 378 ENSFAEL 398
>TC209651 weakly similar to UP|Q9C887 (Q9C887) Myosin-like protein;
97843-94399, partial (51%)
Length = 1013
Score = 32.7 bits (73), Expect = 0.29
Identities = 32/112 (28%), Positives = 53/112 (46%), Gaps = 6/112 (5%)
Frame = +1
Query: 281 VLRSEVARLRALNAGFVEERKAFLAQLKAKETSFAKELAEQSSRAEVS---LEFRDRKIS 337
+L++EV +LR+ E +K L LK + T + L + +R + + KI
Sbjct: 430 LLKNEVMQLRS------EVKK--LNNLKQELTGKVQTLTQDVARLQADNQQIPMMRAKID 585
Query: 338 DLEKALGKLRQEAQEESEAKVDLVA---SKDQTLSSMETKLEHLRAELAKKD 386
L + L R E +A ++ + S ++ L SM ++E LRAELA D
Sbjct: 586 GLHQELMHARTMVDYEKKANMEFMEQRQSMEKNLVSMAREVEKLRAELASAD 741
>TC203681 similar to UP|Q9XIV1 (Q9XIV1) Cucumis sativus mRNA expressed in
cucumber hypocotyls, complete cds (Arabinogalactan
protein), partial (40%)
Length = 913
Score = 32.7 bits (73), Expect = 0.29
Identities = 26/89 (29%), Positives = 38/89 (42%), Gaps = 5/89 (5%)
Frame = +3
Query: 157 EKKGNVSPHPVAATSSSDGDRAPVISAAFCGPEETPKP-----NQPGEPSLATPVSSPRA 211
++K +S +A ++ G ++P S P T P P +P PV+SP +
Sbjct: 15 DRKSLLSLAIIAIVVAAVGGQSPAASPTTSPPAATTTPFASPATAPSKPKSPAPVASPTS 194
Query: 212 QDDSVDKPPLSAKGFANREPPYSLSPTPA 240
PP S+ A PP S SPT A
Sbjct: 195 SS-----PPASSPNAATATPPAS-SPTVA 263
>BF068717
Length = 436
Score = 32.7 bits (73), Expect = 0.29
Identities = 19/43 (44%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = +1
Query: 192 PKPNQPGEPSLATPVSS-PRAQDDSVDKPPLSAKGFANREPPY 233
P PG PS ATP + P Q DS KP K N +PPY
Sbjct: 10 PSTQHPGTPSQATPKNKRPHHQADSTPKPQRGPKP-GNPQPPY 135
>TC228623 similar to UP|Q9SA21 (Q9SA21) F3O9.2 protein, partial (85%)
Length = 1082
Score = 32.3 bits (72), Expect = 0.38
Identities = 26/75 (34%), Positives = 32/75 (42%), Gaps = 2/75 (2%)
Frame = -3
Query: 172 SSDGDRAPVISAAFCGPEETPKPNQPGEP--SLATPVSSPRAQDDSVDKPPLSAKGFANR 229
SS AP SA+ P P P+ PG S + P SS + S PP A + R
Sbjct: 468 SSQPPPAPPPSASSAPP---PSPSAPGSTPVSPSCPPSSSTSPAPSPPAPPTVAPPLSAR 298
Query: 230 EPPYSLSPTPAQVAR 244
PP S P P +R
Sbjct: 297 LPPPSFRPLPPSSSR 253
Score = 30.0 bits (66), Expect = 1.9
Identities = 20/82 (24%), Positives = 37/82 (44%), Gaps = 4/82 (4%)
Frame = -3
Query: 163 SPHPVAATSSSDGDRAPVIS--AAFCGPEET--PKPNQPGEPSLATPVSSPRAQDDSVDK 218
+P P A+++ AP + + C P + P P+ P P++A P+S+
Sbjct: 450 APPPSASSAPPPSPSAPGSTPVSPSCPPSSSTSPAPSPPAPPTVAPPLSARLPPPSFRPL 271
Query: 219 PPLSAKGFANREPPYSLSPTPA 240
PP S++ PP + + P+
Sbjct: 270 PPSSSRRVTLNPPPLASTGPPS 205
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.315 0.130 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,844,362
Number of Sequences: 63676
Number of extensions: 276268
Number of successful extensions: 2064
Number of sequences better than 10.0: 140
Number of HSP's better than 10.0 without gapping: 1900
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2032
length of query: 396
length of database: 12,639,632
effective HSP length: 99
effective length of query: 297
effective length of database: 6,335,708
effective search space: 1881705276
effective search space used: 1881705276
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0005.13