Vector Information
The cDNA fragment was inserted at the XhoI-SacI site of the attBpBC vector.
The attBpBC vector is the pBC SK+ vector (Cm resistance;
http://www.stratagene.com/vectors/selection/plasmid1.htm) with sequences
including attB1 and attB2 sites :
CTCGAGCAGCTGAAGCTTTCACAAGTTTGTACAAAAAAGCAGGCTCTTC-(5' end of cDNA),
(3' end of cDNA)-AAAAAAAAAAAAAAAAAAAAGCGGCCGCCCAGCTTTCTTGTACAAAGTGGTGAGAGCTC.
This cDNA clone mbj01002 has an irregular adapter sequence:
CTCGAGCAGCTGAAGCTTTCACAAGTTTGTACAAATAAGC-(5' end of cDNA)