Vector Information
vector_info#M3 (koga san)
The BamHI fragment of mKIAA0681 cDNA was inserted at the BamHI site of the
pTRGmDHFR1 (Tet resistance; This vector was reconstructed from pTRG Target
Plasmid, http://www.stratagene.com/products/displayProduct.aspx?pid=52)
with sequences including attB1 and attB2 sites [EcoRI site (GAATTC)-
ATGACAAGTTTGTACAAAAAAGCAGGCTCA-SmaI/BamHI sites
(CCCGGGATCC)-GAACCCAGCTTTCTTGTACAAAGTGGTCA-(G4S)2 linker
(GGAGGCGGTGGGTCTGGTGGCGGAGGGTCAG-XhoI site (CTCGAG)-320bp
fragment of mDHFR1 gene- XhoI site (CTCGAG)].