Vector Information

 
vector_info#M3 (koga san)
The BamHI fragment of mKIAA0681 cDNA was inserted at the BamHI site of the 
pTRGmDHFR1 (Tet resistance; This vector was reconstructed from pTRG Target 
Plasmid, http://www.stratagene.com/products/displayProduct.aspx?pid=52) 
with sequences including attB1 and attB2 sites [EcoRI site (GAATTC)-
ATGACAAGTTTGTACAAAAAAGCAGGCTCA-SmaI/BamHI sites 
(CCCGGGATCC)-GAACCCAGCTTTCTTGTACAAAGTGGTCA-(G4S)2 linker 
(GGAGGCGGTGGGTCTGGTGGCGGAGGGTCAG-XhoI site (CTCGAG)-320bp 
fragment of mDHFR1 gene- XhoI site (CTCGAG)].